2008年

The starting point of this experiment is to study the structure of soap-film. By changing the height of the triangular prisms, cuboids and pentagonal prisms, I observed the patterns set by the soap within the frameworks. It is surprised that when the proportion of prism is in a specific range, the phase in the middle of the structure will overturn 90 degree and then transmitted into another kind of balance pattern. I named this process “phase transition”. According to the experiment ,we can conclude the change of film patterns within variable prisms are all applied to this regular cycle:： We know the soap films are forever attempting to minimize their energy. It stands to reason that surface tension tend to set up the film in its minimal surface. From the point of Mathematic, each structure should have only one single balance pattern, which is set up on the base of Fermat point and this pattern should stand to the minimize of it’s energy. However, we discovered that in some specific cases, one structure can allowed two kinds of balance films-patterns to exist. In these cases, any small vibration can cause the happening of “phase transition”. To sum up, I presume some structures have two different types of balance film-patterns: one of which stands to the local minimum (in this condition the pattern’s surface area isn’t the smallest); the other stands to the absolute minimum (in this condition the pattern’s surface area is the smallest). There is an energy valley separate local minimum from absolute minimum. The second pattern (local minimum) will appear when the structure is blocked from attaining its absolute minimum, but surface intention is not powerful enough to support the film jumping over the energy valley. In this condition, if we works on the structure (such as blowing), which would provide the film of energy to cross the valley, and then phase transition take place. Vice versa, we can also force the film to jump from absolute minimum to local minimum and phase transition will occur as well. In a word, phase transition can happen in each two way, which connects the two types of balance pattern. This report lays stress to find out the condition of phase transition. We also analyze the structure of soap-film by its included angles and surface area in hope to go deep into the science of soap-film. 我們實驗的出發點在於研究泡膜的立體結構。藉由改變正立方柱的高，觀察其平衡薄膜形式，意外的發現當正立方柱的邊長比在某個範圍時，泡膜結構中央會瞬間90 度翻轉，形成另一種平衡型式，我們將這個過程命名為面轉變(Phase Transition)。為了進一步了解面轉變發生的相關因素，我們設計了一連串的實驗，針對正三角柱、正四角柱、正五角柱、正六角柱發生面轉變的時機和條件分析討論。此外，我們還分析了泡膜結構中膜與膜夾角的特性、最小表面積和表面能之間的相關性，對於泡膜的立體結構做了一系列深入的探討。

在實驗中學2007 年校內科展，參展作品《三角形中的切圓》的研究中，研究三角形內的切圓時，發現連續切圓的圓心與拋物線的軌跡有關。於是去查資料，在偶然的情況下，翻閱《平面幾何中的小花》時，接觸了「六圓定理」。因為覺得這問題非常有趣，於是便著手證明（見報告內文）。 又發現，當移動六個圓中的起始圓時，總是在某種情況下，六個圓會重合成三個圓。繼續研究其重合的狀況，發現了馬爾法蒂問題（Malfatti's Problem）的一種代數解法。 當我試著推廣六圓定理至多邊形時，發現奇數邊的多邊形似乎也有如六圓定理般圓循環的狀況，於是著手證明，但目前尚未證明成功。而偶數邊的多邊形則無類似的結果。 ;In 2007 National Experimental High School Science Exhibition, one of the exhibit works, "Inscribed Circles in Triangles", shows that the centers of the consecutive inscribed circles has something to do with the parabola's trajectory. To learn more about inscribed circles and parabolas, I referred to literature. By accident, I am faced with the problem on six circles theorem, in the book The Small Flower of Plane Geometry(平面幾何中的小花). Out of my interest in this problem, I tried to prove it. The other results are as follows: With the initial circle of six circles moved, in certain circumstances, the six circles merge into three. Further in studying this coincidence leads to an algebraic method to solve the Malfatti's Problem. Applying six circles theorem to the odd-number-sided polygons exists the same characteristic. It indicates that the inscribed circles will form a cycle. However, it hasn’t been successfully proven. The even-number-sided polygons show no similar results.

流體旋轉時，外圍及底部流體，因槽壁及槽底摩擦力的影響，流速較慢，相對的壓力也較大，導致外圍的水流會轉入中心。發現本實驗的渦流為強迫與自由漩渦組成。實驗中，探討f(轉動器的頻率)、H(總水深)、y(?入深度)、R(轉盤半徑)四者與角形數間的關係。若y、R 愈大、H 越小，隨著f 的增大，可觀察到的形狀邊數越多；反之，若y、R 愈小、H 越大，則f 愈高，所形成的圖形半徑愈大，易超過轉盤，不易觀察。依白努利方程式，外層水流的流速較慢，而內層水流的流速較快，故外層壓力大而內層壓力小，水會由外往內流，而此渦動流於轉動液面產生的剪力，可能為產生N 邊形漩渦的主要原因之一。流體旋轉系統中，因轉動而產生流體離心力與內外層壓力差交互作用下，於某特定相關的因素條件下，形成特定角形數漩渦，是本實驗的重要發現。When fluids are in rotation, fictitious force given by the container brings about the relative decrease of speed of the bottom and outer layer of water, which causes its pressure to increase, and water to spin inward, resulting in a vortex motion with N-corner polygons formed at the surface of the rotating plate. During this experiment, we discover that the vortices consisted of free and forced vortex and the polygons vary as control parameters f(rotation frequency), H(height of fluid), y(depth of the plate), and R(radius of the plate) change. The larger y and R are,the smaller H is, the more corners show up as f increases. On the contrary, the smaller y and R are,the larger H is, few polygons are identified since the rotating radius of polygons are larger than the plate. According to Bernoulli’s principle, smaller velocity of the outer-layer water causes water pressure to increase and water to spin inward. During this process, shear force is developed at the surface of the rotating fluid, which we believe is the main cause of N-corner polygons. In a rotating system, the interaction of centrifugal force and differential pressure causing a certain Ncorner polygon to be formed under different controlled parameters is our main discovery.

RNA 病毒在複製過程中容易產生錯誤，導致其族群具中有很大的遺傳歧異度，累積的錯誤再加上選汰的壓力造成往後之變異。由於RNA 基因體之病毒變異較大，使得RNA 病毒在單一寄主上具有quasispecies 的特性，提供病毒產生新基因體的機會以適應環境或演化成新病毒。例如流行性感冒病毒與之前造成恐慌的嚴重急性呼吸道症候群病毒(severe acute respiratorysyndrome,SARS)以及禽流感病毒 (avain influenza virus) 皆為RNA 病毒，意味著RNA 病毒知不穩定性，並容易造成一些目前我們無法及時反應的危害。大部分的植物病毒又為RNA 病毒，本研究將以竹嵌紋病毒 ( Bamboo mosaic virus , BaMV )及其衛星核酸 (satellite RNA, satBaMV)為材料，進一步探討核?酸序列之變異對其族群在複製競爭上的影響。

本研究探討運用磁場來達到非接觸煞車的功能，本實驗採用兩種方式來探討磁煞車力，分別為馬達有外加電流及沒有外加電流的情況。首先本實驗提供一穩定的電源使鋁盤轉動，觀察加上磁場及把電源切掉後鋁盤轉速的變化。實驗發現，當馬達沒有外加電流時，磁煞車力與轉速及磁場平方皆成正比；馬達有外加電流時，電流差會與轉速平方差成正比。探討磁煞車力與厚度及介質的關係，實驗結果發現，渦電流常數與厚度成正相關，且當兩片鋁片中夾有介質時，渦電流常數較小。 This experiment is based on the magnetic brake’s practical uses and braking forces. We want to calculate the braking force, and also examine the factors that cause the braking force to differ.We attached a metal disk to a motor to make the disk rotate, then we control the distance between the magnet and the metal disk, therefore measuring the relativity of the distance and the rotational speed. We discovered that when the metal disk received a large quantity of the magnetic field (close distance), the breaking force and the rotational speed increased. On the other hand, when the metal disk received a small amount of the magnetic field (far distance), the breaking force and the rotational speed decreased. The magnetic braking force will convert into kinetic energy, thus, by using this connection and also by increasing the electric current to measure the resistance, we calculated the magnitude of the magnetic braking force. Hence we perceived an inverse ratio between distance and the braking force, that is to say, the closer the distance, the stronger the magnetic braking force; the further the distance, the weaker the magnetic braking force.

Two soldiers walk on a checkerboard. They can only walk one step once a time and two directions, front and left, are decided randomly. The gunshot is the column and row where a soldier is located, and one will die if he enters the gunshot area of the other. To treat the probability of winning, we first study the cases of 1×n, 2×n, 3×n, and 4×n rectangles iterately. Then we establish a general form of the probability of winning in a general n×k rectangle by using recurrence technique and generating function, respectively. Finally, we extend to the general n×m×k cuboid case to obtain the first soldier’s probability of winning.在一個長方形的棋盤中，兩士兵行走，每一次只走一步，而且上和左兩個方向是隨機的，射程範圍是所在的此行和此列，而進入他人射程範圍則死亡。探討其獲勝機率，從1×n 、2×n、3×n、4×n 矩形的情形逐步研究，並分別運用遞迴式的技巧及生成函數，導出 n×k 矩形中先走士兵獲勝機率的一般式。更進一步地，我們也獲得了n×m×k 立體空間先走士兵的獲勝機率。

This Project is inspired by the situation incurred by pedestrians, which for the most part are students who need a crossway in order to obtain public transportation or to get to the school; the difficulties that are faced by the personnel to exit the parking lot as well as the students who have a vehicle and to help those parents who drop and pick up their children at the school. At the same time, we would like to reduce the amount of contaminated gas emissions that are emanated into our environment. As consequence of the emission of toxic substances, the air contamination can cause side effects such as the burning of eyes or ears, throat irritation and itching and or respiratory problems. Under determined circumstances, some chemical substances that are found in the contaminated air can produce cancer, congenital malformation, brain damage and disorders to the nervous system, as well as, pulmonary damage and harm to the respiratory tract. For the present investigation it has been suggested as a primary goal: The development of a device, in this case a traffic light, which has the objective to reduce the previously mentioned traffic/security problems that arise upon entering and exiting the institution. The secondary goal is to have a friendly ecological impact within our community. This device was built and tested during a month to obtain figures and demonstrate benefits reported. The device should be low maintenance, it should have a long lifetime and, be simple enough to be operated by those who use it. Among the benefits found, the safety of the students, the prevention of accidents such as: car crashes and run overs, etc. Our studies indicate that per week it is consumed an average of 2,020.16 liters of gasoline, in schedules of 13 hours (from 7:00 AM to 8:00 PM) to lessen this figure would have a good ecological impact since all the hydrocarbon emission are harmful to health.

機械加工過程中往往遇到形狀複雜工件，無法用一般虎鉗夾持進行加工。若需加工複雜工件時，需使用V 形槽、壓枕……等等夾具加以輔助，但有些夾具根本無法夾持。若用特殊夾具需拆除原有之虎鉗，而且還必須校正，工作繁雜又浪費很多時間。 本設計之優點為不需更換虎鉗，直接放在虎鉗鉗口即可夾持不規則的物體，利用正向力的作用夾持而不打滑，輕易達到夾持時之穩定和足夠之夾持力，以達迅速、不需使用特殊夾具、不需再校正、可當平行塊之多功能夾具，使複雜形狀之工件加工簡單化、迅速化之設計。;When handling workpieces in complicated and irregular shape in the mechanical process, users are unable to make it with ordinary vises. V-block and clamping block might help, while some others do not work at all. In such cases, the user has to tear the vise apart and then do some correction, which is complicated and time-wasting. The strength of this design is that there is no need to replace the vise. The user just puts this device on the vise clamp to clamp the irregular object. The vertical clamping force makes the piece at work stable and allows no slipping. With this device, no special fixture or further correction is needed. It can also be used for a parallel block if necessary. In other words, as a fixture of multiple functions, the device makes the processing work simpler and more efficient than ever.

Adducin蛋白在細胞骨架的調節上扮演著重要的角色。然而，近來有許多研究指出，骨架蛋白也會出現在細胞核並參與轉錄調控，因此本研究的目的即在探討adducin蛋白是否會進入細胞核中，並參與轉錄調控或具有其他功能。在本研究中，我們將綠色螢光蛋白(GFP)標示的adducin質體DNA，利用轉染技術送入老鼠纖維母細胞株NIH3T3中表現。NIH3T3細胞原本並無adducin蛋白的表現，在共軛焦顯微鏡下觀察，野生型的GFP-adducin蛋白會表現於細胞核與細胞質中。由於adducin蛋白尾端序列攜有可能往核內運輸的訊號，於是將位在此一訊號中的離胺酸718及離胺酸719進行突變，結果發現此一突變株只能在細胞質中表現。此外，蛋白磷酸脢C(protein kinase C)已知能磷酸化adducin蛋白在絲胺酸716及絲胺酸726的位置，於是假設其磷酸化是否與其在細胞內的分布有關。將adducin的絲胺酸726置換成丙胺酸，並不影響其在細胞內的分布。然而將絲胺酸716置換成丙胺酸後，則完全只在細胞核中表現。由於adducin可分布於細胞核，因此我們懷疑adducin蛋白可能與細胞分裂有關，於是本研究利用流式細胞儀分析adducin轉染後NIH3T3細胞的細胞週期。流式細胞儀的分析結果顯示，攜有GFP-adducin或其突變株的細胞與未經轉染的NIH3T3細胞的細胞週期並沒有顯著差異。其次，為了避免因轉染的效率不高而造成統計上的誤差，我們利用顯微鏡追蹤技術觀察攜有GFP-adducin的細胞株，結果顯示攜有adducin突變株的NIH3T3細胞株仍能正常分裂。再者，因為adducin能與細胞骨架中的肌動蛋白結合，所以adducin不同的分布位置可能影響細胞附著與細胞展延的效率。細胞展延試驗的結果顯示，adducin及其突變株對細胞附著與細胞展延的效率並無明顯的影響。本研究的結果證明，adducin的確帶有往核內運輸的訊號，其在細胞質中的分布可能也同時受到絲胺酸716磷酸化的影響。然而adducin的功用似乎與纖維母細胞的分裂與展延無明顯的關聯性。Adducin, an actin binding protein, is known to play an important role in the regulation of the membrane cortical cytoskeleton. More and more evidence indicates that proteins involved in the cytoskeletal regulation could also reside in the nucleus and participate in gene regulation. Thus, the goal of this study is to examine whether adducin is expressed in the nucleus and involved in certain nuclear events. In this study, adducin and its various mutants were fused with green fluorescent protein (GFP) and transfected into mouse NIH3T3 fibroblasts which do not have endogenous adducin for monitoring their subcellular distribution under a laser scanning confocal microscope. The wild-type GFP-adducin was found to be present both in the nucleus and in the cytoplasm. The COOH-tail of adducin contains a motif analogous to the nuclear localization signal (NLS). Mutation of two lysine residues (lysine 718 and lysine 719) located within this motif abolished the nuclear localization of adducin. Moreover, adducin is known to be phosphorylated by protein kinase C at serine 716 and 726. Substitution of adducin serine 726 with alanine had no effect on its subcellular localization. In contrast, substitution of adducin serine 716 with alanine led to only nuclear expression. Nuclear localization of adducin renders it possible that adducin may be involved in the regulation of cell division cycle. For cell cycle analysis, flow cytometry was applied. The results of flow cytometry indicated that expression of adducin and its mutants in NIH3T3 fibroblasts did not affect their cell cycle progression. To further examine the effect of adducin on cell division, NIH3T3 cells transiently transfected by adducin were monitored by time lapse video microscopy. The video clearly showed that the cells with GFP-adducin underwent cell division to generate two daughter cells. Since adducin is well known to bind to actin and thereby regulate microfilaments, we wondered that expression of adducin in NIH3T3 cells might affect their adhesion and spreading onto extracellular matrix proteins. The results of cell spreading assays showed that adducin appeared not to affect cell spreading. In conclusion, our results demonstrate that the subcellular distribution of adducin is likely regulated by two signals, one is the nuclear localization signal and the other is the phosphorylation status of the serine 716. However, enforced expression of exogenous adducin in fibroblasts such as NIH3T3 cells does not alter their cell cycle or cell spreading on fibronectin.

本研究首先確認培地茅根系具有碎形之基本特性，再進一步以方格覆蓋法計算之碎形維度來分析培地茅根系在不同時間及環境因素下的生長。主要探討碎形維度與抓地力之關係，並設計以實際根系模型來加以模擬，並發展出一可描述抓地力與碎形維度及深度關係的方程式。我們的結論為：（1） 經由方格覆蓋法之計算，培地茅此種植物，不管是整個根系或單枝根，均具有碎形基本特性，適合進一步實驗研究。（2） 碎形維度會隨著培地茅生長時間增長而增加，並且在自然光照及30℃左右會有較大值，而種植於土壤中根系發展較廣，其碎形維度比種植於沙耕中來的高。（3） 實驗結果顯示，抓地力受碎形維度及根系深度兩因素影響，而培地茅根系對土壤有較強的抓地力，推測是因為兩者根系皆又深又長，土中培地茅根碎形維度較大，接觸面積較廣，而又進一步以矽膠模型做實驗驗證。（4） 矽膠模型之目的在於減少難控制之自然變因，實驗之前，測量了根系模型與洋菜凍之基本性質，實驗結果顯示抓地力與碎形維度及根系深度皆呈正向關係，可用數學方程式加以描述。This project is mainly a research into the fractal dimension of the vetiver root system. First, we confirm the vetiver root system has the basic fractal structure by checking its self-similarity, then using box-counting method to calculate fractal dimension. We begin with a fundamental investigation into the relation between different time and environmental factors and fractal dimension. Then we move to our main point: the relation between fractal dimension and its pull-out resistance. In the next step, we make a fundamental silicon model, simulating the vetiver root system, to continue our experiments. In the end, we develop a formula that can describe the relation between its pull-out resistance, roots depth and fractal dimension. Here are our conclusions: （1） After using box-counting method to calculate fractal dimension, we discover that not only the whole vetiver root system but also a single vetiver root has the basic fractal structure. （2） Fractal dimension increases when time goes on. Also the value of fractal dimension is larger in natural sunlight and the temperature at about 30℃.The vetiver root system grows more widely in soil than those in sand. That’s why it has larger fractal dimension. （3） Data shows that its pull-out resistance is influenced by both fractal dimension and the depth of the roots. The vetiver roots, in the meantime, show greater pull-out resistance than some other plants. Thus we draw the assumption that the vetiver root system grows deep and wide, and in natural soil its fractural dimension is greater and reaches greater area. Therefore, a silicon model is constructed to further confirm the findings of the experiment.（4） The design of the silicon model is to reduce the uncontrollable variables in nature. Before starting the experiment, we measured some basic characteristics of the silicon model, including density and angle of repose. Furthermore, the experiment demonstrates that pull-out resistance and fractural dimension have a commensurate mutual relation: the stronger the pull-out resistance, the wider the fractural dimension and the deeper the root system. Thus we derive a math formula to describe this relation.