利用自製頻譜儀研究蜜蜂的發聲系統
本研究利用麥克風與相關電腦設備，結合成自製頻譜儀用以觀測多種情況下蜜蜂的聲音頻率。若將蜜蜂的翅膀加以修剪，可測得有不同的頻率，解析頻率發現「翅膀為主要發聲點，但去除翅膀仍有高頻的發聲，且有三種不同的頻率。」將蜜蜂置於不同溫度下，解析頻率得知「一定溫度範圍內，溫度越高蜜蜂發聲頻率越高，反之亦然。」幼期在胸部塗顏料使絨毛無法生長，去除雙翅後，仍有頻率相近的發聲，得知「胸部絨毛不是造成高頻的原因。」靜置５分鐘，待蜜蜂停止發聲後，剪去腳、挑弄蜜蜂會發出高頻，得知「情緒是引起高頻的原因。」將蜜蜂的翅膀加以修剪，分別放回蜂窩口，發現「同一族群蜜蜂可用發聲頻率來辨別同伴。」比較義大利蜂及中華蜜蜂，得知「在多種情況下中華蜜蜂發聲頻率皆較義大利蜂高約７０Hz。」因此本實驗之結論並不受蜂種影響。The study, capitalizing on a hand-made frequency divider, the microphone and computerized equipment, observes a variety of frequency of sound given off by bees. We read different frequencies from the apparatus when the bee’s wings were trimmed. Analyzing it, we discover that the bee’s winds are major source of its sound, but it still gives out high-frequency sound when the wings were completely cut off.” After analyzing the frequency, we discover that within a certain temperature range the higher the temperature is, the higher the frequency is, and vice versa. In one experiment, we painted the thorax at its pupal stage to stop the bee from growing fine hairs. Even though the wings had been removed, it still gave out high-frequency sound. We, therefore, conclude that fine hairs on the thorax have nothing to do with the making of the sound. In another experiment, bees were placed in an undisturbed environment until they are completely silent. Then, some of the bee’s legs were cut off, while others were provoked. And all the bees make high-frequency sound in the process. We make a hypothesis that emotion could be the cause of bees’ sound-making. The bees with different trimmed wings were put back to the beehive; the bees can still recognize one another by the different sound frequencies. If we compare A. m. ligustica with A. c. cerana under different conditions, we find that the frequency from the latter is about 70 Hz higher than that form the former.
旋光性介質對電磁波影響的分析與討論
This experiment mainly aims at three kinds of solution - Dextrose, Saccharose, and Fructose. By changing its temperature, density, length of tube, as well as different wave length factor of polarized light, we observe the influence of the direction of polarization by those factors. The experimental result showed as follow. The Dextrose and the Saccharose can cause the polarized light with the rotary direction of clockwise, so both are ‘dextrorotatory’. The Fructose can cause the polarized light with the direction of counterclockwise, so it is the ‘laevorotatory’. For the Dextrose, when the\r temperature is lower than 20℃, the direction of polarization has changed observably, but doesn’t have any rule. When the temperature is higher than 20℃, the direction of polarization increase slowly. For those three kinds of solution, when\r density increased, the polarization increased observably. When the polarized light passed through the solution with longer path, the direction of polarization has more change. When the wave length of the polarized light changed, the direction of polarization has been changed observably. When the wave length of the polarized light is shorter, the direction of polarization change increased.本實驗主要針對葡萄糖、蔗糖、及果糖等三種旋光性溶液，改變其溫度、濃度、容器管長、以及不同波長的偏振光等因子，觀察這些因素對偏振方向所造成的影響。實驗結果顯示：葡萄糖與蔗糖會使得偏振光的偏振方向以順時針旋轉，屬右旋性之光學異構物；果糖會使得偏振光的偏振方向以逆時針旋轉，屬左旋性之光學異構物。若溶液為葡萄糖，當溫度低於20℃時，偏振光的偏振方向會有明顯的改變，但無規則可尋；當溫度大於20℃時，偏振方向旋轉角位移則以非常緩慢的方式增加。當此三種溶液之濃度增加時，偏振光的偏振方向有明顯遞增的現象。此外，當容器長度越長（即偏振光在介質中的行程越長）時，偏振方向的改變亦越明顯。當偏振光的波長改變時，偏振光的偏振方向有明顯的變化，且當偏振光的波長越短，偏振方向的改變越大，似乎與波長呈反比，但此結果與理論值（即旋光度與波長平方成反比）仍有一些差距。
鉤盲蛇(Ramphotyphlops braminus)捕獵四部曲
Feeding behavior is the action which animals depend on maintain livelihood. Snakes usually use the three following ways to catch their preys: winding, venom-releasing and pressing their game to death. However, previews study is rare about the feeding behavior of Ramphotyphlops braminus. This puzzles us, prompting us to do in-depth research on it. When performing an experiment, we will use the camera with infrared function to record entire experiment and the obtained data will transform the graph. Our result shows the feeding behavior of R.braminus is a new way to catch their game, and the minute process of this special way is also written down in our report. We hope that the result can let everyone be clear about Ramphotyphlops braminus of soil ecosystem status, and it is an essential contribution for building the archive of Family Typhlopidae. 攝食行為是動物賴以維生的行為。在蛇類中，常見的捕捉方式有：纏繞、 使用毒液、壓斃等三種類型。但，文獻中卻沒有任何有關於鉤盲蛇(Ramphotyphlops braminus)的捕食行為。這使我們感到疑惑，並想深入探討。在實驗進行當中，我們使用紅外線攝影機將實驗全程錄製下來，並將所得的數據轉化成圖表。而其結果顯示鉤盲蛇(Ramphotyphlops braminus)的捕食行為是一種全新的模式，這種模式的詳細過程也被我們全部收錄到報告中。我們希望做出來的結果能讓大家對鉤盲蛇(Ramphotyphlops braminus)在土壤生態系中的地位更加了解，而且對於建立盲蛇科(Typhlopidae)資料庫有實質的貢獻。
外觀數列
The Look and Say sequence is produced by describing the appearance of the previous row. For example, start with “1,” which can be described as “one 1,” and therefore the second row is “11,” which is "two 1s," making the third row “21,” the fourth row “1211,”and so on. The main goal of this study is to work out the exact formula for this sequence, which means given the row number n, we can know at once what the n-th row is without having to start from the first row and doing the look-and-say iteration for n-1 times. Some of the methods used include dividing groups, repetition and cracks. The formula we derived speeds up the calculation and gives us a better understanding of the look and say sequence.「外觀數列」為依照外觀產生下一列的數列，第一列為「1」，第二列描述第一列「1 個1」而為「11」，第三列則描述第二列「2 個1」而為「21」，第四列「1211」，依此類推。本研究針對外觀數列的各項數學性質作研究探討，並由此推導出外觀數列的一般式，即給定第n 列就可知道該列的內容。我們運用了分組、重複性以及裂縫的方法分析數列，最後得到了其一般式，此一般式有助於運算速度的加快以及我們對數列性質的了解。
抑制水果黑色素形成之新理論及研發美白保養品之新概念
PPO 是一種含銅的多酚氧化? (E. C. 1. 14. 18. 1)，主要是將酚類 (phenol) 氧化成二酚類(diphenol)，更近一步的變成quinones，後者是一種不溶於水的褐色聚合物，在植物中造成褐化最主要原因，然而在人類皮膚則產生黑色素 (melanin)。我們提出有一種揮發性的抑制劑存在動植物體內，此種抑制劑會抑制生物體內PPO 的活性。植物在收成後，揮發性的抑制劑逐漸消失，導致內生性的PPO 活性逐漸上升，因此植物便會產生深褐色的斑點。在本研究中，我們以玉蘭花作為例子，以生化酵素動力實驗、部分純化黑色素抑制劑來解釋揮發性抑制劑在植物體內的存在及其作用，更進一步對水果快速褐化提出一種新的理論。研究發現PPO 的褐化反應就像是人體內酪氨酸? (tyrosinase) 的催化反應，酪氨酸?可以使人體產生黑色素而累積在皮膚上形成黑斑，利用〝人工皮膚〞模擬揮發性黑色素抑制物的作用，證明，防止PPO 抑制劑之揮發可能在未來美白保養品工業裡扮演一極具潛力的角色。Polyphenol oxidase (PPO) or tyrosinase (E.C. 1.14.18.1) is an important and ubiquitous enzyme responsible for browning in plants and melanization in animals. PPO is a copper-containing enzyme that catalyzes the chain-oxidation from monophenol or polyphenols to o-diphenols and subsequent o-quinones. The resulting quinones are large wate-insoluble polymers with dark brown color. We proposed that volatile inhibitors are associated with the plant PPO and block the PPO activity in vivo. While post-harvesting the volatile inhibitors evaporate, the endogenous PPO is then activated and therefore instantly produces dark quinone pigment. In the present study using magnolia flowers as an example, we show the presence of a potent volatile inhibitor(s) for PPO in plant. The novel finding clarifies the mechanism involved in the browning phenomenon of post-harvesting for most fruits. Since the PPO is also know present in human as tyrosinase responsible for the formation of “darkening spots” on skin, the finding of evaporation of potent PPO inhibitor may be potentially used as a strategy in developing a novel cosmetic product.
線鋁之情-以陽極氧化鋁模板製作氧化亞銅奈米線
我們使用陽極氧化鋁(AAO)模板來製備銅及其氧化物的奈米線。以硫酸銅和乳酸配製電鍍液，利用氫氧化鈉水溶液(NaOH)將其pH 值調整到12，供以不同電壓，可電鍍出銅及氧化亞銅奈米線。在較高電壓下可製備出銅奈米線，而在較低電壓下可製成氧化亞銅奈米線，若使用中間電壓則能製得銅及氧化亞銅的混合態。利用x 光繞射分析儀(XRD)來分析其結晶構造、使用場發射掃描式電子顯微鏡(SEM)以得知其表面形貌。電鍍出的奈米線直徑約60 nm。奈米線的長度可藉由調整電鍍時間或電壓來控制。在製作IC 內部導線方面，銅奈米線深具開發潛能；在提升太陽能電池的轉換效率、製作可見光光觸媒方面，氧化亞銅奈米線極具前瞻性。We electrodeposited copper and cuprous oxide (Cu2O) nanowires with anodic aluminum oxide (AAO) templates. Both Cu and Cu2O nanowires could be prepared with an alkaline cupric lactate solution, which was adjusted to pH 12 using a 6 M NaOH, when supplied with different electrolytic voltages. Cu nanowires could be prepared when a higher voltage was supplied, and Cu2O nanowires could be prepared with a lower voltage. A mixture of Cu and Cu2O nanowires could be prepared with a supply of a voltage in between. X-ray diffraction (XRD) is used to determine the phase composition, and scanning electron microscopy (SEM) is employed to characterize the morphology of the nanowires. The length of nanowires can be controlled by adjusting the time spent on electrodeposition and the voltage supplied. The resultant diameter of the nanowires was about 60 nm. Cu nanowires are promising materials for making the conductive wires in IC, and Cu2O nanowires hold great promise for improving the conversion efficiency of solar cells and manufacturing visible-light photocatalyst.
Vitamin C in Cold and Flu Drinks
This chemistry project was designed to measure the vitamin C levels within liquid cold and flu remedies, and see if they had been accurately stated on the package. A range of Cold and flu drinks was selected, including one made with only cold water (Lemsip Original, Lemsip Max, Effer-C-Cold Water, Relief and Lemting) .The procedure used to carry out the experiment was a redox titration, each trial required two titrations, with the first being a blank titration to determine a sodium thiosulfate concentration in the absence of vitamin C. The following Drinks had more Vitamin C than stated on the packaging – Lemsip Original (19.4%), Lemsip Max (22.1%), Effer-C (17%) and Relief (8.8%). Lemting was the only drink made to directions that had less Vitamin C than stated by 31.8%. Two conclusions can be drawn from these results, the first being that Lemting has the most inaccurately recorded mass of vitamin C and is also the only drink with less vitamin C than stated. The other interesting result is that Effer-C (the only drink made with cold water) had 170mg of extra vitamin C. Of the other drinks that had more vitamin C, Lemsip Max was next with an extra 22.8mg. Early research had showed that Vitamin C was affected by high temperatures (above approximately 70oC) and, as cold and flu drinks are commonly made with hot water the Vitamin C levels might change when they were made up. This poses a further possible research question which is, in making cold and flu remedies with hot water is some of the available Vitamin C being destroyed?
本土藥材金銀花的研究與分析
本實驗以薄膜色層層析（TLC）、高效能液相層析（HPLC）分析等化學方法，進行金銀花品種差異的鑑識；此外，配合生藥學的顯微鏡檢視，如中藥材組織鏡檢、藥材粉末鏡檢等比對，以期找出辨別金銀花品種的方法。研究至目前為止，由金銀花之薄膜色層分析的Rf值（0.225、0.425、0.7、0.85、0.95）可確認出金銀花藥材，並得知金銀花藥材中皆含有綠原酸的成分；以高效能液相層析的圖譜與成分峰的積分面積可用來判別金銀花的品種，並從質譜分析瞭解成分含量；進行生藥學的藥材粉末組織鏡檢，發現無法作為金銀花藥材的分類憑藉。未來將持續延伸實驗，朝向中藥奈米化與一般粉末在藥效、成分上差異的比較，並進行金銀花萃取液的抗菌作用試驗，瞭解不同品種之金銀花藥材在藥理作用的異同，接續著奈米化藥材的應用與實踐。Using thin layer chromatograph (TLC) and high performance liquid chromatography (HPLC), we can study how to differentiate the species variation of honeysuckle; beside, based on the observation of biopharmaceutical microscope, such as comparing the histology of Chinese herbs and its powder, we suggest that we could differentiate the species of honeysuckle. From the present, firstly, we could distinguish the honeysuckle from other herbs by the Rf value of TLC(0.225, 0.425, 0.7, 0.85, 0.95), from which we find that all honeysuckles contain the component of Chlorogenic acid. Secondly, we could tell the species of honeysuckle according to the map of HPLC and the peak area after integration, as well as the integrants of honeysuckle by way of LC-Mass analysis. Thirdly, while studying the histological analysis based on the observation of biopharmaceutical microscope, we found that it shows no difference between all the honeysuckles; thus, it fails to be a scientific method used to distinguish the herb honeysuckle. However, in the biochemical experiments of honeysuckles, we found honeysuckles from different sources and the place of origin shows difference in their antibiotic effect, showing the importance of local medicine. When it comes to my future work, in order to extend my experiments on honeysuckles, I would compare the nano-scale honeysuckle powder with normal-sized one in their clinical effects and components.
單細胞浮游藻類對紫外線防禦機制之探討
在先備知識中，我們知道在缺少營養鹽及紫外線傷害下，浮游藻類的葉綠體會因為過氧化物（R.O.S.）的增加而受到破壞，進而影響光合作用的進行，甚至導致死亡。所以確實了解常見浮游藻類生理狀態和環境的影響，以期待未來可利用大幅度提高浮游藻類生產力的方式有效降低溫室效應的影響為本實驗的主要目的。故實驗設計針對兩種常見的海洋種浮游藻類（Tetra、Ske），在不同紫外線光譜（UVAB、UVC）的照射下，觀察R.O.S.的產生量和T-T dimer的表現狀況，並對照兩者之間的關係。結果我們發現：綠藻（Tetra）和矽藻（Ske）在UVAB、UVC 的照射下皆會產生R.O.S.，且綠藻產生的量較少；但在UVC 照射下皆有DNA 損傷（產生T-T dimer）。故推估並不是綠藻（Tetra）擁有紫外線的特殊防禦機制，而是能較有效地代謝R.O.S.。As we know, under the condition of unorganized salt’s shortage and the harm of the ultraviolet ray, the phytoplankton’s chloroplast will be destroyed because of the increasing peroxide (R.O.S.). Furthermore, the ultraviolet ray will have an effect on the process of photosynthesis, and even result in the death of phytoplankton. So, we intend to promote the production of phytoplankton in order to lower the influence of greenhouse effect by probing into the environmental influence on the physiology of phytoplankton. The experimental is designed to observe two common marine phytoplankton: Tetra and Ske. By close observing Tetra and Ske exposed to different wavelength of ultraviolet way (UVAB and UVC ), we contrast the production of R.O.S. with the appearing of T-T dimer. We observe that both Tetra and Ske will produce R.O.S. after being exposed to UVAB and UVC , but Tetra produce less than Ske, and that UVC will do harm to both the DNA of Tetra and Ske (producing T-T dimer). Based on the result of the experiment we estimate that Tetra can catobolize R.O.S. efficiently instead of having a unique defensive mechanism against ultraviolet ray (UVAB and UVC under discussion in this experiment.)