2004年

動脈硬化是個致病率和致死率相當高的慢性發炎疾病，為台灣十大死因之一。在病理過程中血中濃度過高的膽固醇為動脈硬化的一大危險因子，其會誘發一連串的發炎反應驅使血管壁內皮細胞功能喪失，血壓上升，平滑肌細胞增生等。Statin 是臨床上十分有效的降血脂藥物，雖然其作用機制已知在於抑制膽固醇合成酵素 HMG-CoA reductase 而有降血脂功效，但近年來探討 statin 在抗發炎方面的作用也漸受重視。nitric oxide synthase (NOS) 代謝產物如 nitric oxide (NO)，cyclooxygenase (COX) 代謝產物如 prostaglandin (PGE?、PGI?)，及 heme oxygenase-1 (HO-1) 代謝產物如 carbon monoxide (CO)，均有文獻指出可以改善血流，而可能在動脈硬化上扮演保護角色。相反的，matrix metalloproteinase (MMP) 的表現會誘使更多的免疫細胞浸潤到血管壁，並增加動脈硬化斑破裂，引起栓塞和中風的發生。在此實驗中，我們利用培養的大鼠主動脈血管平滑肌細胞作為研究材料，發現了 statin (lovastatin 、pravastatin、atorvastatin 、fluvastatin) 具有一些和降血脂無關的直接保護血管壁能力。包括會增加 interleukin-1β (IL-1β) 所誘導 iNOS 蛋白的表現及NO 的產生; statin 本身會增加 COX-2 和 HO-1 蛋白的表現及 PGE? 和PGI? 的產生，及抑制 MMP-2 和 MMP-9 蛋白活性的表現。此外分析調控 iNOS 基因轉錄最為關鍵的基因轉錄因子 NF-κB，發現適量的 statin 會增加 IL-1β 活化NF-κB 的作用。值得一提的是雖然適量 NO 有維持血管恆定的功能，過量時則會造成血壓過低休克的現象，這就是細菌感染後因內毒素 lipopolysaccharide (LPS) 作用引發敗血性休克的主要原因之一。為更進一步釐清 statin 是否會影響受細菌感染病人的生命危險，我們也探討 statin 對LPS 作用的影響。結果發現 statin 反而會抑制 LPS 誘導大量 iNOS 蛋白的表現，NO 的產生及 NF-κB 的活化。這些新的實驗結果提供更多證據支持 statin 可以藉由維持血管舒張，減緩血管壁的發炎反應，穩定動脈硬化斑的作用，以有效控制動脈硬化各個病程的進展。這發現能讓我們更透徹明白 statin 的作用，且對將來研發 statin 在心血管疾病方面新的臨床治療用途是有所助益的。 ;The 3-hydroxy-3-methylglutaryl (HMG)-CoA reductase inhibitors, statins, are potent inhibitors of cholesterol synthesis and have wide therapeutic use in cardiovascular diseases. Recent evidence, however, suggests that the beneficial effects of statins may extend beyond their action on serum cholesterol levels. In this study, we investigated the effects of lovastatin, pravastatin, atorvastatin, and fluvastatin on cultured rat vascular smooth muscle cells. We found statins can inhibit LPS-induced iNOS expression and NO production, while they can potentiate IL-1β-elicited responses. Moreover, statins themselves can stimulate COX-2 expression, PGE?, PGI? formation, and HO-1 induction. In contrast, statins can inhibit basal and IL-1β-induced enzyme activities of MMP-9 and MMP-2. In studying the activity of NF-κB, which plays an important role for iNOS gene induction, we found that statin can increase IL-1β-induced NF-κB activity, while inhibit that induced by LPS. All these results suggest that stimulation of iNOS expression in the presence of IL-1β, togeth1er with the increased COX-2 and HO-1 expression might contribute to the beneficial effects of statins in atherosclerotic process in terms of vasodilation and inhibition of smooth muscle cell proliferation. The inhibition of MMP activity might enhance plaque stability and reduce the development of atherosclerosis. All these results strengthen the pleiotropic actions of statins in anti-inflammation and anti-atherosclerosis.

The aim of the work is inventing the way in which the power of the ICE is the same and consuming of the fuel decreases. The following methods of investigation were used: analysis of the experience of the improvement of ICE, modeling, the brainstorming, methods of Decition Theory of Invention’s Tasks (DTIT). In this work Ivan Semyonov based on a hypothesis that if the non-supporting combustion exhausts will be drawn with the vacuum from cylinder fuel for the same power it needed less. The practical meaning of this work is in the attempt of studying the question of improving the ICE for getting and making the more perfect ICE.

Now Artificial Neural Networks using on the basic math is fewer. This paper is to suggest the Linear equation of the basic math using the BP Artificial Neural Networks. The BP Neural Networks have power ability for learning and can approximate any function, and regularity can be found to solve the linear equation. A good sample is one of the important elements for learning of Artificial Neural Networks. Generally, the samples are a lot of amount for the resolution of Linear equation. This paper is to use the principle of two points decide one line for the samples. The experiment shows that this method curtails many samples. Furthermore we also use Artificial Neural Networks to solve the problem of point-slope form. The experiment result is very satisfactory, and it offers some idea for the basic math using Artificial Neural Networks.目前人工神經網路較少用於基礎數學方面的求解，本文針對基礎數學直線方程式提出BP 人工神經網路應用於求解直線方程式，運用其很強的學習能力、(輸入向量和其對應的目標向量來訓練網路、逼近函數)，尋求規律來求解直線方程式；而良好的樣本是人工神經網路學習的重要條件之一，一般解決直線方程式需要大量樣本，本文利用二點決定一直線的原理來解決樣本問題，實驗結果顯示，這一方法成功的縮短了可觀的學習樣本，此外我們也運用BP 人工神經網路來求解點斜式的直線方程式問題，實驗結果是可行的，並且為人工神經網路用於基礎數學提供了一些思考方向。

安非他命的濫用在台灣是非常嚴重的公眾健康及社會問題。安非他命會導致一連串的行為異常，包括在中腦紋狀體內釋放多巴胺及阻止多巴胺回收來增加使用者的活動力。由於安非他命會對腦細胞造成傷害，本研究的目的為探討低劑量、無立即毒性之安非他命(類似於人類使用習慣)長期施打下，是否會對C57BL6 小鼠大腦紋狀體內的蛋白質表現有影響。因此利用西方點墨法分析施打低劑量安非他命(2 到6 mg/kg) 約一星期之後，C57BL6 小鼠的大腦紋狀體中一些重要蛋白質(包括腺.酸受體A2A-R、第五亞型腺.酸環化.AC5、caspase-8 及PARP) 的表現是否有改變。實驗結果顯示，低劑量安非他命處理對這些蛋白質的表現並沒有明顯的差異。但利用二維電泳法可看到有少許蛋白質，在經過安非他命處理下有顯著的差別，如KIAA0193 homolog 、GOS-28、gammacrystallin A、malate dehydrogenase 和phosphoglycerate mutase isozyme B (PGAM-B)。這些蛋白質中，malate dehydrogenase 和PGAM-B 與代謝和產生ATP 有關，但前者是增加的，而後者減少，推測安非他命會影響神經細胞的能量代謝，因此長期施打安非他命對紋狀體造成的影響值得進一步探討。;The wide spreading use of amphetamine (AMPH) in Taiwan has become a serious public health and social problem. AMPH evokes a series of behavior abnormality including enhanced locomotor behavior by releasing dopamine and inhibiting dopamine-uptake in the striatum. Since AMPH is known to cause brain damage, the purpose of this study is to investigate the expression of several important proteins in the striatum of C57BL6 mice after chronic treatment with low and non-toxic dosages of AMPH (mimicking the common usage pattern of AMPH addict). C57BL6 mice were daily IP-injected with various dosages of AMPH (0 to 6 mg/kg) for one week. Expression levels of A2A adenosine receptor (A2A-R), adenylyl cyclase type V (AC5), caspase-8 and PARP in the striatum were analyzed by Western blotting analysis. Most proteins examined were not affected by this 1-week AMPH treatment. By the aid of two-dimensional gel electrophoresis, expressions of a few striatal proteins (such as KIAA0193 homolog, GOS-28, gammacrystallin A, malate dehydrogenase and phosphoglycerate mutase isozyme B (PGAM-B) in AMPH-treated mice were altered. Note that malate dehydrogenase and PGAM-B are two enzymes involved in energy metabolism and ATP generation. Interestingly, the former was increased and while the latter was decreased in AMPH-treated mice. Collectively AMPH may affect the energy metabolism in neuronal cells. These results suggest that the injury induced by long-term AMPH exposure warrants our further concerns and investigation.

在利用電腦螢幕來瀏覽圖形式資訊的時候，常常受限於螢幕的空間，沒有辦法在顯示\r 資訊整體結構的同時顯現細節部分的資料，目前的使用者介面所採用的方法有放大（zoom\r in）、捲動（scrolling）、開啟多個視窗（multiple view）等方法，這些方法雖然可以呈現出資\r 料的細節部分，但是仍有其個別的缺點存在，放大的方式會有遮蔽的情形；捲動的方式無\r 法同時地呈現整體結構；開啟多個視窗的方法使得使用者的眼睛必須在這些視窗間來回的\r 移動，造成麻煩。\r 魚眼鏡頭是一種短焦距、大視角的相機鏡頭，鏡頭成像的時候，越接近鏡頭中心的物\r 體會越放大而越遠的部分會越縮小，藉著發掘魚眼鏡頭的成像函數，我們發展出了一種新\r 的使用者介面，在瀏覽圖形式資訊的時候，能夠顯示整體的結構，並隨著滑鼠游標的移動，\r 以不開啟新視窗及無遮蔽的方式，即時地將想要觀察的部分局部放大以展現細部的資料，\r 這種使用者介面將具備現有方法的優點而無其缺點。\r Browsing the global structure of a large graph in limited screen space has the drawback that details\r are often too small to be seen. The most common solution provides a scrollable view. This shows full\r details at the region currently visible through the view, but hides the rest of the global structure.\r Alternatively, zooming into a part of the graph does show local details but misses the overall structure of\r the graph. The multiple views approach, one view of the entire graph and the other of a zoomed portion,\r has the advantage of seeing both local details and overall structure, but has the drawback that parts of the\r graph adjacent to the enlarged area are not visible at all in the enlarged views.\r A fisheye camera lens is a very wide angle lens that magnifies nearby objects while shrinking distant\r objects. It seems to be a tool for seeing local detail and global structure simultaneously. By means of\r exploring fisheye camera lens, we develop a user interface for browsing graphs using program analog of\r fisheye lens. Thus, our method seems to have all the advantages of the other approaches without suffering\r from any of the drawbacks.\r \r

本實驗是控制奈米碳管的成長條件，研究奈米碳管的成長機制、電子場發射特性。奈米碳管的物理性質會因捲曲度(helicity)、管壁厚度(單層或多層)、管徑所影響，而不同的捲曲度，會形成不同電性(金屬性或半導體性)的奈米碳管。由此可知，探討奈米碳管的成長機制，以控制奈米碳管的穩定成長，及如何長出電性或光學性質相近的奈米碳管，是極為重要之事。為了研究奈米碳管的成長機制，我們使用微波電漿輔助化學氣相沈積法 (Microwave plasma ehanced chemical vapor deposition) ，在市售的縫衣針針尖上，成長奈米碳管，這個技術能夠經由吸收微波達到局部加熱針尖的方式，再透過控制微波功率、通入氣體的種類、壓力的變化以及催化劑的使用，能夠達到有效控制奈米碳管的成長條件。我們改變三個參數：催化劑的使用、氣體種類(Ar+CH4 或N2+CH4)及壓力，之後利用SEM 觀察奈米碳管的成長情形；再測量場發射(測量電性)，討論不同成長的條件與場發射電流關係。

Our traditional bow is a kind of horn bow which appeared at the side wall in the sepulcher\r around A.D 600. A lot of traits of the horn bow have been handed down to us in an\r unwritten form. This is the reason why I would like to research and investigate the several\r potential capacities in the field of Physics, such as structures, materials and efficiency.

這是一系列關於水蒸氣冷凝為極細微水珠的實驗。其中可以歸納為三大部分，第一部分是基礎實驗，將水蒸氣導引至親水性介面上，觀察冷凝水珠的結構。雖然看似簡單平常，但是卻發現：不同溫度的水蒸氣，其冷凝最初始的細微珠粒，尺寸相同；爾後溫度高者，堆疊速率較大，以至於最後同時呈現的水珠大小不一，尺寸不同！ 第二部分，是針對冷凝水珠自我組裝機制的探討。實驗是將水蒸氣導引至密度小於1的高分子溶液上，並藉由揮發性溶劑快速揮發，將水珠粒「分層保留」以便更深入了解「解構」後的水珠群聚機制。在這組實驗中得到兩張有趣的圖片： 在討論時，我是從對流機制切入，嘗試解構上面兩張圖。 第三部分的實驗，是將水蒸氣導引到磁場及靜電場上，觀察冷凝的機構。這部分呈現出來的結果，推翻了一般「水分子為電中性應該在電場與磁場中不受影響？」刻板觀念，實驗呈現水分子：不但在電磁場上不易長大同時也有固定的散佈模式(assembly pattern)。同時也觀察到：水分子在正電場形成的凝結水珠較為均勻，在負電場則表現出較大親水性特質。這部分的實驗對日後研究細胞膜上水分子通道應有助益。 I have tried to ask a famous math professor if he can create a formula describing the ordered array of water droplets. “Then, I should study Physics first!” He said. Condensation is the thing we live with, being found everywhere, passing without notice. But we never know when it dose start? By coalescence, water droplets grow bigger, but are not round again. We used the polymer film as template and designed the solution lighter than water, so the minute droplets will sink to the bottom and layer by layer. After seconds we may have multilayers of ordered array. This experiment presented here is actually the diary of the growth of water droplets through condensation, upon volatile fluid, magnetic field and electric field. Through convection, it discusses the self assembly mechanism of water droplets and peep into the uniformity of the size of water droplets. In this experiment, convection and magneto-electric force did play important roles in the self assembly mechanism of water droplets. The topic is mostly concerned as we are surrounded by magneto-electric waves in today’s world. This is the first step in discovering the homogeneous state of water droplets, providing insights into the self assembly mechanism of water droplets with nano sizes.

此研究是探討在目前全球因聖嬰現象後北極暖化，造成溫度持續在三十年內以每年上升攝氏零點五度，目前多數資料以顯示，對於地球內的生物生態產生了微妙的變化，在本文中將引述著名雜誌─科學人雜誌所刊登之關於全球暖化造成的生態環境影響；然而早在三億五千萬年前就已存在地球上的古老生物─蟑螂，順利的度過了多次的大滅絕，走過冥霜與煉獄。但是否會因為暖化作用而造成其生態影響呢？他又會不會成為少數存留並且大量繁殖的征服者呢？所以我們開始查詢白堊紀之資料，在研究、討論並製作改造完成實驗室氣溫控制冷熱溫差調節器，並從專業研究蟑螂生態的業界專業實驗室取得同一時期的實驗蟑螂物種，以期待本實驗更能具有更高的正確可信度；於特殊自行改造的觀察箱內進行整個實驗，已改變溫度並測量其進食狀況，瞭解蟑螂在溫度變化下的生態狀況。\r \r It’s easy to find the cockroach at any corner, such as school or house. They also hide in the refrigerator and stove.Thus,we are curious why they have durable vitality. This is the reason why we want to uncover the mysterious veil. According to data, we are curious about the environment of the cockroach and the temperature.Therefore,we want to imitate the situation of the ecosystem temperature at that time and inquire into its mystery. The purpose of Research is to make the violent changes, then discussing the meal which has attained its biggest existence rate. This uncontrolled experiment will influence the accuracy by factors.Therefore,we go to visit the laboratory personally and obtain some species of cockroach. Through the professional explanation and introductions, we make sure the direction of this experiment further. In this experiment we measure their appetite and the controls of the temperature everyday. They almost can crawl quickly along any material. We adopt the professional suggestion to measure with CO2 and O2.When the cockroach inhales CO2,we can observe the construction of the each part carefully. Through long-term observation, the food of the cockroach decreases, when the temperature rises to 20.6℃ or declines to 16.3℃,and it will stop moving when the temperature rises to 31.8℃ or declines to 8.7℃. When the temperature rises rapidly or reduce more than 15℃,the cockroach will look for shelter. Besides they easily get fainted when cockroach inhales CO2 without soil. The dinosaur were all buried underground, but why can the cockroach survive up to now? Probably, large land is their savior! Our conclusion is (1)The temperature that cockroach can exist from 49℃ to 3℃.(2)The suitable environment of cockroach growth is between 28.5℃ and 25℃.(3)The cockroach maintains their existence by eating under the low temperature 20℃ to 15 ℃.(4)Above 32℃ and under 7℃ the antenna is close to ground, its life is weaker.(5)The cockroach almost can live at any dilemma. But it can’t keep the prosperous life when it’s short of water.(6)From the above cockroach will be king of the world forever.

This study tries to reveal how the velocity of flow influents the orientations and scattering patterns of the two-valve shells (we use Pecten in this study) during depositing processes by comparing the laboratory studies with the field observations. We learn from the laboratory experiments that the shells deposit at random directions when they are too crowded and are oriented to a certain direction when they are more scattered. We also find out that the shell fossils whose dorsal sides towards downstream with upward concaves are most likely to move. On the contrary, the shells whose dorsal sides towards upstream with downward concave are the most reluctant to move Examinations of the outcrop of the bedding of Pecten fossils, which are located near Long Dong, the northeastern tip of Taiwan, show that a lot of the shells settled along a certain direction and most of their concaves are upward. However, some of the shells with obvious orientation have downward concaves, which implies the depositional environments were under high current velocity. And the numbers of the shells whose dorsal sides are oriented to Northern East are about equal to those whose dorsal sides are oriented to Southern West. This result coincides with the results of the laboratory study. However, the experimental results can apply to the field analysis under certain assumptions.本研究就影響貝殼排列的水速作試驗，尋找水流和貝殼排列的關係，並和地層中實際的貝類化石做比較，藉以回推古老沉積環境。結果發現大量貝殼集中的情況下，貝殼間彼此的影響力會大於單純的水流沖擊；當貝殼較分散時，可以看出貝殼的頭部皆會一致朝向水流的來向作排列。而最適合移動的型態是凹口向上且頭部不朝水流來向的貝殼，而最穩定的型態是頭部朝水流來向的凹口向下貝殼。觀察實際地層，貝類化石大致上也有方向性的排列且正反面貝殼都有，可以推得當時沉積的水流大，故有足夠的動能讓貝殼重新排列。但由於試驗環境定和自然環境有所差異，仍有限制存在。