利用 Verapamil 引發斑馬魚胚胎心衰竭模式並探討臨床心衰竭用藥 Dapagliflozin 和 Valsartan 之成效與機制
本研究利用 Verapamil 誘導斑馬魚胚胎心衰竭模式,並探討 Dapagliflozin 對斑馬魚胚胎表皮離子細胞的調控機制,以加深對 SGLT2 inhibitors 機制的了解。受精後第四天的斑馬魚在暴露於Verapamil 24小時後,除了抑制卵黃囊吸收以及造成心包膜水腫以外,對心臟整體功能(HR, EDV,ESV, SV, EF, CO)具負面影響。以粒線體染劑標記離子細胞,發現Verapamil使其密度上升,使用掃描式電子顯微鏡觀察,則可看到離子細胞頂端開口有明顯的萎縮,影響到正常功能。以抗體標記染色的方式檢測不同離子細胞亞型,顯示 Dapagliflozin 使富含 Na⁺-K⁺ ATPase 的 HR 細胞和富含 H⁺-ATPase 的 NaR 細胞密度上升。同時,心臟功能診斷標誌物的 mRNA 水平(naap, nppb,gata4, vmhc)暴露於Verapamil後上升,促進離子細胞代償性上調。
Mattress Ventilation System
One of the worldwide hidden problems with lack of attention is Bedsores. Simply, These are ulcers, that happen on the areas of the skin that are under pressure while lying on the bed for a prolonged time. This can be infected to anyone in the world. The common group who face these bed sores are..... •Elderly patients, •Spinal cord injury patients, •Stroke patients, •Coma patients, •People who have faced accidents Bed sores develop when the blood supply is cut off for more than 2-3 hours to the skin, (Position changing time depends on the patient's condition) The continuous pressure is the cause for that and also the temperature generated between the skin and the bed surface increases the metabolism of the tissues. When the skin temperature increases by 1°C, the risk of bed sores increases approximately 14 times. So, The skin temperature in the range of 29.4°C to 37.1°C is correlated with the tissue damage score. Not only that shearing and moisture are the other considerable factors for this problem. This infection has 4 stages. Sometimes in the 4th stage of the bed sores can cause death.