全國中小學科展

2024年

軒嵐諾颱風2022與卡努颱風2023冷水坑成因之能量通量分析

本研究首先觀察 2022年軒嵐諾颱風與 2023年卡努颱風表面的海水溫度較低的區域,即冷水坑,以及颱風中心附近 200公里的平均風速。進而以表面海水溫度較低之區域訂定為冷水坑研究的空間範圍,計算冷水坑在六個方向(東、西、南、北、上、下)的能量收支,包括分析:水平與垂直的傳導、可感熱、水平與垂直的溫度通量。 各個能量途經在颱風滯留時全部顯示冷水坑在向外界吸收能量,同時從能量的收支量判斷出海水主要以水平溫度通量、熱傳導和可感熱進行能量交換,而東方與北方為能量向外流出的區域,且冷水坑的能量吸收在颱風滯留的第一天達到高峰,並且高峰持續至颱風滯留的最後一天,在颱風離開冷水坑後,向外匯出的能量不斷減少,約在滯留後兩天才轉為能量流入。

利用氧化應激法結合膠原蛋白微島嶼誘導人類臍帶間質幹細胞分化

再生醫學透過控制幹細胞分化以應用於修復受損或是建構新組織。目前誘導幹細胞分化的方法多為添加成長因子的化學誘導法,然而此方法具有高成本和費時等缺點。在本篇研究中,我們以人類臍帶間質幹細胞,作為誘導分化對象,使其貼附於具最適化圖形的膠原蛋白微島嶼,結合大氣電漿及 H2O2 二種氧化應激法刺激細胞,探討誘導 WJ-MSC 幹細胞分化的最佳條件。在本研究中,我們發現單獨使用大氣電漿處理可使成骨分化率增加,而大氣電漿處理再加上星形微島嶼培養細胞時,則更顯著的提高成骨分化率。未來嘗試以 H2O2 誘導幹細胞分化, 並進一步探討大氣電漿和 H2O2 提高脂肪分化率的條件、影響分化的機制,期望能此新技術取代傳統化學誘導法,對組織工程做出貢獻。

以限制酶酵素切位多樣性檢驗臺灣入侵紅火蟻基因體重組事件

入侵紅火蟻( Solenopsis invicta,以下簡稱紅火蟻) 起源於南美洲巴拉那河流域,其對於農業、畜牧業、甚至對人的健康都具有高度危險性及破壞性。由於國際貿易地興 起,紅火蟻目前已於包含台灣在內多個國家中被發現並在當地造成危害。為了對抗紅 火蟻造成的影響,瞭解紅火蟻的基因將是個有效的方向。故希望從決定紅火蟻的性別 決定基因之候選位置中探索其重組率以更精準地定位性別決定基因的所在位置。本篇 研究選用紅火蟻其中兩條等位基因序列,分別標記為 H04、H07。對於紅火蟻來說,正常情況下單套個體將發育成雄蟻、雙套且為異型合子的個體將發育成為雌蟻(職蟻或處 女蟻后),然而同型合子個體則會發育為不孕的雙套體雄蟻。本實驗利用限制酶酵素切 位多樣性( Restriction Fragment Length Polymorphism, RFLP )技術,使用 MEGA 軟體將H04、H07 序列之同源序列做排序後,使用 Ape 軟體找出其候選酵素切位並篩選出於不同等位基因序列上具有多樣性之切位後,利用聚合酶鏈鎖反應( polymerase chain reaction, PCR )、酵素酶切割及電泳凝膠技術,找出理應為異型合子之雌蟻中只具有 H04 或 H07 序列其中一個訊號之個體,即代表該區域曾發生過基因體重組事件而應被排除出性別決定基因的候選位置。我們成功確認 H07、H04 的 90 個樣本中,同型合子共有12 個、異型合子共有 78 個,並且雄蟻皆只偵測到一個訊號,符合預設。未來繼續縮短範圍,直至找尋到決定紅火蟻重要基因序列。

代謝物 2'O Methyluridine 對乳癌細胞株的影響及作用機制探討

在近期的臨床觀察中,我們發現腫瘤微環境有許多共存微生物,本研究透過生物資訊學發現微生物代謝物 2'O Methyluridine 可能有促進乳癌細胞生長的功效, 便設計實驗進一步解析兩者之間的作用機制。 本研究探討 2'O Methyluridine 對 MCF-7、T47-D 兩種常見乳癌細胞株的作用機制,發現 2'O Methyluridine 對 T47-D 的生長與細胞群落形成具有促進效果,而 MCF-7 的生長則是受到抑制,且 2'O Methyluridine 可能造成乳癌細胞中編碼粒線體 ATP 合酶亞基的基因之表現量降低。 本實驗的研究結果可提供乳癌治療的新方向,若微生物代謝物對於不同種類的癌症細胞株有不同的反應,我們可以針對患者的腫瘤細胞預先進行分析,並同時針對微生物與癌細胞進行治療,或許可達到更佳的療效果,減輕化療的副作用。

Raising the Microscopic Guardians from the Ocean-Pioneering A Natural Solution for Vibrio Control in Mariculture Using Marine Bacterivorous Ciliates Euplotes sp.

水產養殖在應對傳統漁業過度捕撈和資源下降所帶來的挑戰中扮演著重要的角色。隨著水產養殖作為全球食品安全和經濟發展的關鍵組成部分不斷嶄露頭角,它面臨著與細菌感染相關的挑戰,以及對抗生素過度使用的日益關注,這既對環境又對健康構成風險。我們的研究深入探討了海洋食微生物對生態系統的重要性,特別關注了在水產養殖背景下的 Euplotes sp.。為此,研究致力於建立 Euplotes sp. 的標準化培養方法,並評估其作為水產養殖中對抗細菌感染的生物防治的潛力。進行的實驗探討了 Euplotes sp. 在水質凈化方面的有效性以及對海洋細菌生長的影響。研究結果為水產養殖和廣泛的水產品生產領域發展環保和可持續實踐提供了希望。

利用水凝膠進行亞硝酸鹽的檢測

亞硝酸鹽與對胺基苯甲酸 (4-Aminobenzoicacid, PABA)反應生成重氮化合物,並與 N-(1- 萘基) 乙二胺二鹽酸鹽 (N-(1-Naphthyl)-1,2-ethanediamine dihydrochloride, NED) 進行偶聯反應,進一步生成紫色、褐色的化合物,使水凝膠的表面從無色轉變成化合物的顏色。隨著亞硝酸鹽濃度的改變,水凝膠成色的效果也會有所不同,這也導致了其表面灰階的變化。重氮化偶聯反應可用於亞硝酸鹽的比色測定,亦被證明可用於實際水樣本中的亞硝酸鹽檢測。

Wibrazz

"Blindness keeps you from things, deafness keeps you from people" (Helen Keller) Wibrazz is a communication tool that can be placed inside sportswear. Two versions have been developed. The simpler one allows hearing-impaired footballers to compete in the league with other athletes. The referee is given an additional device to give a signal when he blows his whistle. The hearing-impaired footballer then senses the signal from the device he is wearing and knows that he must pay attention to the referee. The complex version speeds up communication between the coach and the players during training sessions. It allows the coach to send simple messages to his players using his smart device. The athlete senses the signal from the device and acts on what has been previously discussed (e.g. a long signal means, "Everyone come to me!") With over 70 million deaf people worldwide, and 2-4 out of 1000 people in the United States who are functionally deaf, this can affect an individual's mental and physical well-being, and it is therefore a pressing issue to provide these athletes with the means to develop their talents in a traditional team environment. In addition to the organisations within countries, the ICSD is present on the international stage. Their importance is demonstrated by the fact that the 2023 Deaf Football World Cup featured teams from countries such as the United States, Germany, England and Japan.

Quantitative environmental DNA metabarcoding for the enumeration of Pacific salmon (Oncorhynchus spp.)

Understanding species abundance is critical to managing and conserving planetary biodiversity. Pacific salmon (Oncorhynchus spp.) are keystone species of cultural, economic, and ecological importance in Alaska and especially Southwest Alaska. Traditional methods of enumerating salmon such as weirs and visual surveys are often costly, time-intensive, and reliant on taxonomic expertise. Environmental DNA (eDNA), which identifies and quantifies species based on DNA they shed in their habitats, is a potential cost- and time- saving alternative. The relative ease of collecting eDNA samples also enables citizen scientist involvement, expanding research coverage. Currently, more research is required to define eDNA’s potential and limits. This project investigates whether quantitative eDNA metabarcoding can accurately quantify the abundances of six fish species: the five Pacific salmon species plus rainbow trout. Water samples were collected from eight creeks in the Wood River watershed of Southwest Alaska. eDNA metabarcoding and subsequent bioinformatics processing produced a read count for each species. These were compared to visual survey counts, taken to be the true counts for the purposes of this study. Data analyses showed a positive, linear relationship between visual survey count and eDNA count for sockeye salmon. The regressions were significant for both the early (p = 0.089) and late (p = 0.030) sampling dates when 𝛼 = 0.10. eDNA detections of non-sockeye species generally corresponded to visual survey observations of species presence or absence. Overall, the results of this study support eDNA’s potential to be an alternative or supplement to standard methods for the enumeration of fish species.

Development of Electrical Shielding system for auto dust removal for solar PV modules

Currently, Large-scale solar PV systems installations are taking place in the desert environment and space to harness abundant solar energy effectively. Dust accumulation on the surface of photovoltaic panels (PV) is the most stignant problem for photovoltaic panels, as dust deposition reduces PV problem energy generation efficiency significantly. Thus, the removal of dust from the PV panels has become very important to increase the rate of energy efficiency by the PV panels. The dust particles could be reduced using traditional dust cleaning techniques. But, in the harsh and hostile desert environment, these approaches which requires a lot of water, complicated mechanical instruments, enormous logistics, and personnel. Electrodynamic dust shielding (EDS) system has been developed at our laboratory to remove dust particles from the surface of the PV panels. The EDS system has been successfully tested for dust mitigation. The unique features of our EDS System is that it is cost effective, easy to install and no manpower required as it is an unmanned system. The design and construction of a single-phase electrodynamic dust repulsion device built locally in KSA is demonstrated in this study. This work showed that the optimized electrode design and electrical parameters, such as AC source voltage and frequency, maximized the EDS system's dust mitigation effectiveness. A perfect balance was achieved between the geometrical and electrical parameters of the EDS system, resulting in a dust removal efficiency of up to 92±1 ℅.

Whose feather is that? A cross-views between a naturalist and a molecular biologist

Identifyingthespeciesorsexofabirdbasedonafeatherfoundinnatureisoftenchallenging,evenwith the help of reference books. However, determining the presence of a rare species in a habitat using an indirectpresenceindicator,suchasafeather,canhelpinimplementingspecificmeasuresforpreserving the species. The aim of this study is to investigate whether DNAgenotyping is better than specialized books when identifying bird feathers. Toanswerthisquestion,Icollectedfeathersinthewildand,withthehelpoftwobooks,triedtoidentify theirspeciesandsex.Then,assistedbyDrGwenaëlJacob(UNIFR),Iisolatedtwogenesinnineselected feathers. The investigated genes were the CHD gene for sexing and the COI gene for species identification.Todothis,theDNAwasfirstextractedfromthefeathers,purified,andamplifiedbyPCR. Subsequently,anelectrophoresis wasperformedtosexthe samples andcheckthatthe PCRamplification hadworkedproperly.Finally,thesamplesweresequencedbytheMicrosynthlaboratory(St-Gall),and the obtained sequences were entered into the NCBI database. Acomparisonoftheresultsobtainedwitheachofthetwodifferentmethodsshowsthattheidentification with specialized books was fairly successful. 56% of the species identification made with the books were indeed confirmed by genotyping. DNAanalysis provided a different result only for feather #16. However,33%ofgeneticidentificationfailed,eitherduetogeneticmaterialqualityorlaboratoryerrors. Asitwaspossibletoidentifythesexofonlyonesample(feather#14)withthebooks,itwasnotpossible tomakeatruecomparisonofthetwoapproaches.However,asgeneticsexingworkedwell(onefailure, feather #28), it can be inferred that genetic sexing is more effective than using books. This work demonstrated that DNAis not infallible and that sometimes books are equally effective in identifyingbirdspeciesfromafeather.However,insexingbird,DNAremainsmoreefficient.Thus,one can conclude that DNAgenotyping is not superior but rather complementary to specialized books for identifying bird feathers.