臺灣

日照影響花芽分化及生長試植物生理學上的重要課題,根據相關文獻指出,蛋白質類型的變化是影響開化的重要關鍵,大豆是短日照植物,分別以長日照(LD18hr/DAY)和短日照(SD6hr/DAY)處理,抽取葉片中的蛋白質以SDS-PAGE.2D-SDS-PAGE分析,SDS-PAGE的實驗姐果顯示出在分子量25.2 32.9 38.6 20.6 64.0 100.6 kDa處中的蛋白質SD比LD的量多,但是在分子量16.8kDa以下的蛋白質LD含有高濃度而自SD只有一點,我們使用2D-SDS-PAGE做進一步的分析,SD與LD分為三個區塊討論(SD1-3 LD1-3)有許多蛋白自LD1.LD3出現,但在SD1 SD3中未出現,可能有抑制花芽分化的作用,而在SD2中有多個高濃度蛋白出現,可能在花芽分化上扮演重角色,其中編號P1 P2的蛋白質有較明顯的變化,根據NCBI網站上的資料顯示,P1蛋白質與光敏素C有關,而P2蛋白質則沒有找到分子量等電點類似的蛋白質;The effects of photoeriod on floral initation and develoment is an imporant phyiological subject and have studed ve he past 70 years. Accrding to the reference, polypeptide partten plays an important rolein photoperiodic induction. Soybean s a SD(short day)plant. So we planted them with 6-h(short day,SD)and 18-h(long day,LD)photoperiods. Total proteins n leaves of soyban were extracted from the vegetable,SD and LD. And using SDS-PAGE and 2-D eloctrophoresis to analyze. 25.2,32.9,38.6,50.6,64.0,and 100.6kDa polypeptides in SDS-PAGE were always present in leavess at SD but only a little bands f the polypeptides appeared at LD. And many polypeptides whose molecular weight(Mw)is below 16.8 kDa had higher concentration at LD ad Reduced at SD. We found three different areas(SD1~3,LD1~3) and analyze them. Many proteins were found n LD1,LD3 and reduced in SD2,SD5. Possibly, these polypeptides found in LD1 and LD3 play an important role in restraining floral iniiation in soybean. Many proeins had more concentration in SD2. Probably, they play an important role in photoperiodic induction and floral initiation. We found a protein labeled P1 in SD2 and it is with molecular weight(Mw) of 36kDa at isoelectric point(pI) of5.2, and it may be related to cytochromeC. We also found protein labeled P2 in SD2 and it is with molecular weight(Mw) of 40.2kDa at isoelectric point(pI)of 5.4, and we couldn't find any protein that has the similary Mw and pI in potein database about flowering and daylength.

中文摘要：\r 本文利用溶膠－凝膠法（Sol－Gel method）製備二氧化鈦奈米粒子（Anatase\r 晶相），測定廷得耳效應判斷其是否為奈米粒子，並以二氧化鈦奈米粒子加入水\r 中，以紫外光照射測試其光催化分解水反應的活性，並測試其分解南崁溪河川廢\r 水的能力。結果顯示自製二氧化鈦奈米粒子具有明顯的廷得耳效應，光催化分解\r 水反應因紫外光源功率過低未能呈現，有待改進；分解河川廢水的能力仍在進行\r 中。\r Abstract\r This experiment produces Titanium dioxide nano-particles (Anatase crystal\r phase)by using Sol-Gel method. We prove the particles as nano-order by the Tyndall\r effect And adds Titanium Dioxide into water, illuminate it with special wavelength\r ultraviolet radiation. The activity of its light catalysis reaction to water was tested.\r The capability of the treatment of wastewater in Nancan River by nano-titanium\r particles was also studied. The results show that the powder prepared by this\r experiment has apparently Tyndall effect，the photo-catalysis reaction of water is\r failed due to the weak power of UV light sources. The test of wastewater treatment is\r still processed.

以隨手可得拍攝影像的工具（如智慧型手機、個人數位助理、網路攝影機等）及網際網路的便利性，讓使用者能隨時透過書籍條碼影像向遠端資料庫查詢書籍的相關訊息。本研究以「ISBN書籍條碼」為例，經過條碼影像的擷取、偵測並分割出影像中的條碼、歪斜校正以及辨識後，透過網際網路，從ISBN書籍資料庫中查詢出該書籍的相關資訊，讓我們體驗行動通訊科技所帶來的便利。在程式的實作上，除了使用現有影像處理技術中的霍夫轉換與邊緣偵測外，也搭配上自行設計的變化量分配圖，使得ISBN條碼的辨識能力大為提升。此外，為了將使用者查詢時等待回應的時間降至最低，採用了記憶體直接存取的方法來處理影像外，也針對程式執行時的瓶頸部分，以平行處理的機制來加速，經過在Intel i5 M560四核心處理器上的實際測試，一幅900×675像素的影像，平均只要花費1.08秒即可辨識出ISBN條碼，相當的迅速。

本研究利用麥克風與相關電腦設備，結合成自製頻譜儀用以觀測多種情況下蜜蜂的聲音頻率。若將蜜蜂的翅膀加以修剪，可測得有不同的頻率，解析頻率發現「翅膀為主要發聲點，但去除翅膀仍有高頻的發聲，且有三種不同的頻率。」將蜜蜂置於不同溫度下，解析頻率得知「一定溫度範圍內，溫度越高蜜蜂發聲頻率越高，反之亦然。」幼期在胸部塗顏料使絨毛無法生長，去除雙翅後，仍有頻率相近的發聲，得知「胸部絨毛不是造成高頻的原因。」靜置５分鐘，待蜜蜂停止發聲後，剪去腳、挑弄蜜蜂會發出高頻，得知「情緒是引起高頻的原因。」將蜜蜂的翅膀加以修剪，分別放回蜂窩口，發現「同一族群蜜蜂可用發聲頻率來辨別同伴。」比較義大利蜂及中華蜜蜂，得知「在多種情況下中華蜜蜂發聲頻率皆較義大利蜂高約７０Hz。」因此本實驗之結論並不受蜂種影響。The study, capitalizing on a hand-made frequency divider, the microphone and computerized equipment, observes a variety of frequency of sound given off by bees. We read different frequencies from the apparatus when the bee’s wings were trimmed. Analyzing it, we discover that the bee’s winds are major source of its sound, but it still gives out high-frequency sound when the wings were completely cut off.” After analyzing the frequency, we discover that within a certain temperature range the higher the temperature is, the higher the frequency is, and vice versa. In one experiment, we painted the thorax at its pupal stage to stop the bee from growing fine hairs. Even though the wings had been removed, it still gave out high-frequency sound. We, therefore, conclude that fine hairs on the thorax have nothing to do with the making of the sound. In another experiment, bees were placed in an undisturbed environment until they are completely silent. Then, some of the bee’s legs were cut off, while others were provoked. And all the bees make high-frequency sound in the process. We make a hypothesis that emotion could be the cause of bees’ sound-making. The bees with different trimmed wings were put back to the beehive; the bees can still recognize one another by the different sound frequencies. If we compare A. m. ligustica with A. c. cerana under different conditions, we find that the frequency from the latter is about 70 Hz higher than that form the former.

我們使用陽極氧化鋁(AAO)模板來製備銅及其氧化物的奈米線。以硫酸銅和乳酸配製電鍍液，利用氫氧化鈉水溶液(NaOH)將其pH 值調整到12，供以不同電壓，可電鍍出銅及氧化亞銅奈米線。在較高電壓下可製備出銅奈米線，而在較低電壓下可製成氧化亞銅奈米線，若使用中間電壓則能製得銅及氧化亞銅的混合態。利用x 光繞射分析儀(XRD)來分析其結晶構造、使用場發射掃描式電子顯微鏡(SEM)以得知其表面形貌。電鍍出的奈米線直徑約60 nm。奈米線的長度可藉由調整電鍍時間或電壓來控制。在製作IC 內部導線方面，銅奈米線深具開發潛能；在提升太陽能電池的轉換效率、製作可見光光觸媒方面，氧化亞銅奈米線極具前瞻性。We electrodeposited copper and cuprous oxide (Cu2O) nanowires with anodic aluminum oxide (AAO) templates. Both Cu and Cu2O nanowires could be prepared with an alkaline cupric lactate solution, which was adjusted to pH 12 using a 6 M NaOH, when supplied with different electrolytic voltages. Cu nanowires could be prepared when a higher voltage was supplied, and Cu2O nanowires could be prepared with a lower voltage. A mixture of Cu and Cu2O nanowires could be prepared with a supply of a voltage in between. X-ray diffraction (XRD) is used to determine the phase composition, and scanning electron microscopy (SEM) is employed to characterize the morphology of the nanowires. The length of nanowires can be controlled by adjusting the time spent on electrodeposition and the voltage supplied. The resultant diameter of the nanowires was about 60 nm. Cu nanowires are promising materials for making the conductive wires in IC, and Cu2O nanowires hold great promise for improving the conversion efficiency of solar cells and manufacturing visible-light photocatalyst.

The purpose of the research was to find the relationship between the fruit fly’s attraction to light (or heliotaxis) and their genes. Fruit flies, because of their short life span of approximately two weeks, and their simple requirements for survival, are ideal candidates for the study. With a specially designed device "Mi Gong" for the experiments, fruit flies could be separated and cultivated in accordance to their preference to specific wavelengths of light. They received cross-breeding and were studied to learn the ratio of the resulting offspring in their preference to the light. It was observed that the majority of the fruit flies were more attracted to violet light. It was also found that the next generation of the fruit fly inherited higher sensitivity to the light than their parents. Knowing that there could be potential margin of errors in the experiments, the results of the study demonstrated that light, as an external source, had limited impact to heliotaxis of fruit fly. The study suggested that insects with heliotaxis, including fruit fly, inherited heliotaxis in their genes. The study also pointed out the potential benefits of employing heliotaxis in many areas especially in the agricultural development. Furthermore, heliotaxis can be utilized to study the difference in the behavior of nightlife insects before and after the invention of manmade light source.這項研究，主要是在探討果蠅的趨光性和遺傳的關係。很多種昆蟲都具備趨光性，我們之所以以果蠅作實驗，主要是因為其生活週期短，大約兩週，且易於培養。實驗內容是以「迷宮器材」來鑑別果蠅對某一特殊色光的偏好，將該群果蠅分離出來培養，令其繁衍後代，觀察其後代對此一色光偏好的比率，我們所用的果蠅大致上對綠色光較敏感，而其子代的敏感度較親代高。因此，我們推論趨光性會遺傳，且受光影響，但並非全由光影響，故這可能是實驗中的誤差成因。雖然此實驗是以果蠅為對象，但亦可假設其它具趨光性的昆蟲，也可能是因遺傳而導致其子代有趨光性，而我們可利用其趨光的一些特性，減少農作物因害蟲類的果蠅而損失，並且希望可以得知,在人造光源還未出現在世上時，夜行性昆蟲是否有差異。

台灣溫泉資源豐富，不同的溫泉泉質適合不同的溫泉藻類生長。溫泉藻屬於極端生物，其應用資源極待研究開發。本研究根據細胞型態、藻膽蛋白種類分析以及rbcL 基因定序等結果推測北投溫泉藻屬於一種溫泉紅藻，其種類近似於Galdieria sp. (Rhodophyta)。此外，本研究利用葉綠素螢光分析儀於野外實測北投溫泉紅藻於不同光強度下，光合作用能力的差異，發現適應於不同光強度的溫泉紅藻，其電子傳遞速率並無顯著的差異。推測其原因可能與北投溫泉紅藻的phycocyanin 含量會隨著光量有所調整，藉此達到最佳的光合作用能力有關。另外，本研究發現隨著光強度的上升，溫泉藻的NPQ 值有上升的趨勢，然而低光區的溫泉藻有較高的NPQ 值，此與一般理論不符。利用HPLC 分析北投溫泉紅藻的類胡蘿蔔素種類組成，其種類分別為Lutein、α-carotein、β-carotein 與Zeaxanthin，未發現Violanxanthin 與Antheraxanthin，北投溫泉紅藻並不俱有葉黃素迴圈。因此根據研究結果，我們認為NPQ 值與色素的關係仍有討論的空間Taiwan is resourceful in hot springs. Various hot spring algae are adapted to different types of hot springs. Hot spring algae applications are still under developing. In this work, based upon the morphology, analysis of phycobiliproteins, and rbcL sequences, the hot spring algae of Peitou is found to be Galdieria maxima (Rhodophyta). In addition, the dependence of the photosynthesis of Galdieria maxima on the light intensity was measured by Diving-PAM. The results show that the light energy availability efficiency of Galdieria maxima adapting to different light intensity exhibited different, although the electron circulate rate differed insignificantly. This suggests that the concentration of phycocyanin in Galdieria maxima may vary to attain optimal photosynthesis. Furthermore, the NPQ of Galdieria maxima increase with the light intensity. However, contradict to the theory; even under the same light intensity, the NPQ of Galdieria maxima was higher at low light zone. The carotene composition of Galdieria maxima was analyzed using HPLC and found lutein, α-carotene, β-carotene, and zeaxanthin. Violaxanthin and antheraxanthin were not present. Therefore, Galdieria maxima do not exhibit xanthophyll. Based on the results of this study, the correlation between NPQ and pigment still needs to be investigated.

在這篇報告中，我們探索了「將錯就錯的Knuth 河內塔問題」。傳統河內塔問題在電腦科學上佔有重要的地位，是一個極具內涵的模型。由於這個模型的深厚數學內涵，使其和巴斯卡三角形建立了緊密的連結，且利用這個緊密的數學連結，設計出復原任意起始狀態的良好演算法。Knuth 河內塔起因於數學家Knuth 在論文[3]中，描述傳統的河內塔問題時所發生的一次筆誤。在這個新的規則之下，我們意外發現Knuth 河內塔存在著一個和傳統河內塔平行的模型，此模型在電腦科學及數學上有著完全不同於傳統河內塔的內涵。我們的研究主要如下：(分別為內文中的四大段)(一) 結構分析。移動環所需要的次數，如何移動環並分析每一次動作所動的環，及每個環何時被動到並給出演算法。(二) 正整數的分割。所有的移動步驟將正整數做了一個新的分割(Partition)；此分割模k之後有良好的循環性質。(三) 費波那契真分數的排序。這個正整數的分割形成一張表，這張表恰好就是分子分母皆為費波那契真分數之排序。(四) 隨意亂排的Knuth 河內塔復原演算法。在Knuth 河內塔的規定下將起始狀態改變，找出良好的復原演算法，並分析。 In this project we study the "Knuth Hanoi Tower", which is motivated by a typo in a paper of Knuth. This inadvertently typo leads to a new rule of moving the discs on the Hanoi Tower (see introduction below for definition). Although seemingly similar to the traditional Hanoi-Tower problem, it turns out that under this rule the "Knuth Hanoi Tower" problem consists of amazing properties, and is totally different from the traditional one. Our study focuses on the following directions: (1) Structure analyzing: We analysis the sequences recording the disc moving and offer enumeration results and recurrsive/non-recurrsive algorithms. (2) Partition of N: The moving sequence forms a partition (a table) of N, which has an amazing congruence property. (3) The order of Fibonacci proper fraction: The row/column of the partition table is, even more amazing, exactly the order when sorting the Fibonacci proper fraction with fixed denominator/numerator. (4) The Restoration of an arbitrary initial state: We offer an efficient algorithm for restoring any initial state of discs. We hope that our study on the "Knuth Hanoi Tower" offers a simple, neat, and new example on the theory of Algorithm, Number theory and Combinatorics.

The paper is to research the method how to prevent the torque down under the higher rotation of the single cylinder with two stroke’s engine and increasing the engine’s torque oppositely. By the process of adjusting （1）changing the weight and the position of the balance block（2） adjusting the exhausting time of the outlet valve（3）to decrease the weight and structure of the crank itself adequately. it is find that the engine’s torque was increased at the situation of higher rotational engine. After the adjusting of the process we have the following result：（1）at the higher revolution zone of the engine. the torque can be maintained and increased（2）the higher revolute efficiently value can be obtained by the correct adjusting of the exhaust time of the outlet valve without increasing the fuel.本研究主要是探討單汽缸二行程高轉速引擎在高轉速時扭力能延遲下降，使扭力增加。我們是利用二行程引擎高效率的結構和現有空間來提升引擎效能。經(1)改變曲軸配重塊之重量及位子移動 (2)調整引擎排氣時間 (3)適度減輕曲軸重量及結構等三項，改良後證明，確定能使扭力提升，和延伸到較高的轉速區。經過調整程序，可獲下列重要結果：(1)在引擎高轉速時，可增加較大的扭力值 (2)藉由適當的排氣時間調整，在不增加燃油供給條件下，可獲得較高的迴轉效率。

超音波應用於臨床已行之有年，而以穿透式超音波打入腦部的想法也已提出，本實驗目的即為測試低強度間歇性超音波達到腦部受傷後治療的可能。本實驗採用星狀細胞培養模式來探討，首先以腫瘤壞死因子α(Tumor Necrosis Factor-α, TNF-α)模擬中風缺氧的狀態，並利用低強度間歇性超音波刺激星狀細胞，然後檢測其培養液中一氧化氮(nitric oxide, NO)的濃度變化，以及大腦衍生神經營養因子(Brain-derived Neurotrophic Factor, BDNF)、神經膠質衍生神經營養因子(Glial-derived Neurotrophic Factor, GDNF)之mRNA 表現的情形。由實驗結果顯示，在TNF-α存在狀況下則能使NO 濃度上升，施打低強度間歇性超音波可促使NO 增加，並提升上述BDNF、GDNF 兩種蛋白質之mRNA 表現，BDNF 可幫助支持神經元生存，促使新神經元和突觸的發展及分化；GDNF 可有效提昇許多類型神經元生存率；而適當之NO 濃度可以調節神經傳遞物質釋放和腦血流，乃至於影響到記憶的產生。我們初步的結果也顯示在TNF-α存在下，超音波可以增加BDNF 及GDNF 蛋白之產生，因此本研究成果顯示低強度間歇性超音波可提供有利於神經元的環境。The ultrasound technology has been widely applied in the clinical practice for many years, such as monitoring of fetus, lithotripsy, rehabilitation after fracture, as well as the ultrasound stimulation of brain tissue, etc. The purpose of this study is to investigate the therapeutic effect of low intensity pulsed ultrasound (LIPUS) on the injured brain. In this study, we adopted the in vitro model of astrocyte cultures. The tumor necrosis factor- α (TNF-α) was used to simulate the condition of ischemic stroke. We used LIPUS to stimulate the cultured astrocytes, then we ssessed the concentration of nitric oride (NO) in the culture medium. We also assessed the expression of messenger RNA (mRNA) of both brain-derived neurotrophic factor (BDNF) and glial cell-derived neurotrophic factor (GDNF). The results showed that NO increased after the addition of TNF- α. In addition, treatment of LIPUS increased the expression of NO, as well as the mRNA of both BDNF and GDNF. The BDNF is able to support the survival of neuron, as well as stimulate differentiation of neurons and synapses. GDNF can also increase the survival of various kinds of neurons. Furthermore, NO may regulate the secretion of neurotransmitters, increase the blood flow in the brain, as well as enhance the processing of memory. Our preliminary results also demonstrated that both BDNF and GDNF protein increased in response to LIPUS in the presence of TNF-α. Therefore, treatment with LIPUS implicated an environment favoring the protection of neurons.