臺灣

Drought and inundation are two unusual natural disasters in Taiwan. The two natural disasters\r have some relation of abnormal rainfall become more and more in Taiwan. So it let me think about\r can climate vicissitudes make the chance of abnormal rainfall become more?\r The study have researched the chance of abnormal rainfall by "rainfall duration" and "total\r rainfall". It collect the day by day total rainfall from 1960 to July 2002, collect locals are Taipei,\r Taichung, Kaohsiung and Hualien. Than enter the data into the computer, let computer calculation\r total rainfall, rainfall days, heavy rain days, pouring rain days and torrential rain days. Then\r analysis the tendency of long-term change.\r According to the analysis, the chance of abnormal rainfall happened become more in Taipei,\r Taichung, Kaohsiung and Hualien. The ratio of Hualien and Kaohsiung is the most obviously. It's\r also find that there temperature and total evaporate became higher, the total sunshine duration\r became lower. Then El Nino have some influence in abnormal rainfal. In El Nino year, total rainfall\r will become lower. When La Nina year, the total rainfall will become more in Taipei and Hualien.\r Then the long influence is clearly in Taipei.\r 乾旱與水災是台灣地區相當常見的二項天災，這二項災害的發生都與異常降水有直接的\r 關係。近年來台灣地區因異常降水造成的天然災害，似乎有逐年增加的趨勢。因此讓人聯想\r 到氣候變遷是否會導致異常降水頻率增加。\r 本研究主要由「降雨時數」與「降雨量」二方面探討異常降水發生頻率。先收集台北、\r 台中、高雄、花蓮四地自1960 年至2002 年七月三十一日之逐日雨量資料，將資料輸入電腦\r 後，統計各站歷年降雨量、降雨日數、大雨、豪雨、暴雨日數，並分析長期變化趨勢。\r 分析結果，台北、台中、高雄、花蓮四地異常降水發生機率，有增加的情形；其中以花\r 蓮及高雄變化的比例最高。再與其他各地氣象要素比較可發現，可能與氣溫及蒸發量數上升，\r 以及日照時數縮短有關。另外聖嬰現象也可能對異常降水有長期性的影響。一般而言聖嬰年\r 雨量減少，反聖嬰年台北、花蓮地區雨量反而會增加。而長期性的影響，以台北地區最顯著。

目的：紫色桿菌（Chromobacterium sp.）是一種生活在土壤及水域環境的革蘭氏陰性菌，它會產生紫黑色的紫色桿菌素（Violacein），因而使菌體與培養液皆呈紫色。Violacein是一種由兩個L-色胺酸（L-tryptophan）分子所聚合成的五環化合物，已被證實具有抗菌以及抑制腫瘤細胞生長的活性。本實驗的目的在探討紫色桿菌是否會利用不同官能基的L-色胺酸，合成不同的Violacein衍生物，並分析這些Violacein衍生物的生物活性。方法：將紫色桿菌培養於液態LB培養基中，分別加入不同濃度的L-tryptophan、1-CH3-L-tryptophan與5-OH-L-tryptophan，於室溫下培養48小時，再用乙酸乙酯萃取Violacein及其衍生物，將萃取出來的紫色桿菌素，用紫外光/可見光分光光譜計和質譜儀進行分析。最後，再將這些萃取物打入斑馬魚受精卵做胚胎毒性測試，並進行試管外DNA剪切能力分析。結果：加入不同官能基的L-色胺酸所合成的產物都是紫色，且在紫外光/可見光的光譜中的最大吸收波長皆相同，質譜儀分析結果則顯示，L-tryptophan 、與5-OH-L-tryptophan的產物圖譜不同、而1-CH3-L-tryptophan的產物則無法分析。斑馬魚胚胎毒性測試結果顯示，Violaecein和1-CH3-Violacein 不會對胚胎發育造成影響，但是5-OH-Violacein則會造成胚胎發育異常。DNA 剪切能力測試也顯示，只有5-OH-Violacein具有剪切DNA的能力，其他則否。重要性：本實驗成功地利用紫色桿菌合成Violacein及其衍生物，並發現了5-OH-Violacein比Violacein 具有更高的生物活性。;Aims: Chromobacterium sp. is a Gram negative bacterium which inhabit in soil and water environments. Chromobacterium produce a purple color compound named Violacein. This colorful compound makes Chromobacterium and its culture mediumin purple color. Violacein is a five rings compound synthesized from two L-tryptophan molecules. Studies have shown that Violacein has anti-bacteria and anti-tumor activities. The purpose of this study is to investigate whether Violacein can be synthesized from different tryptophan analogues, and whether these Violacein derivatives have different bioactivities. Materials and Methods: Chromobacterium sp. was cultured in liquid medium containing different concentrations of L-tryptophan, 1-CH3-L-tryptophan, and 5-OH-L-tryptophan. After 48 hours incubation at room temperature, Violacein and its derivatives were extracted by EA, analyzed by UV/visible spectrophotometer and MS. Violacein and its derivatives were also tested for their embryo toxicity and DNA cleavage activity in vitro. Results: The compounds synthesized from different tryptophan analogues were all purple and have the same maxima absorption wave length in the UV/visible spectra. However, MS spectra of compounds synthesized from L-tryptophan and 5-OH-L-tryptophan were different. The results of zebrafish embryo toxicity tests indicated that violaecin and 1-CH3-Violacein had no effect on embryo development, but 5-OH-Violacein caused development defects. DNA cleavage tests also showed that only 5-OH-Violacein could digest DNA in vitro. Violaecin and 1-CH3-Violacein could not digest DNA. Significances: Results from this study indicate that Chromobacterium sp. can be used to synthesize Violacein derivatives from different tryptophan analogues, and revealed that 5-OH-Violacein is a higher bioactivity compound than violaecein.

This research is mainly in observation with Caenorhabditis elegans ’s genetic mutation caused via nervous system abnormal character. In the study, I the sample have been cultivated purified and add some chemical material EMS to speed up C.elegans mutation. Then based on the character to further analysis what causeof gene deal with mutation and observe the effects in heredity. The research has two stages, on the first stage of study the mainly target is to both search and purify the mutation of C.elegans. The second stage is based on the exploration of mutation’s searching and purifying. Because the certain mutation bodies aren’t easy to find out, the project is still on progress at the beginning of second stage, and we conclude some heredity special cases in preliminary of study. 這個實驗主要是觀察並針對線蟲因為基因的突變所產生的神經系統異常的變異性狀，在實驗中我先將樣品線蟲培養並純化至一定數量，並加入適當藥劑EMS造成其突變，經篩選並分析此性狀，進而找出造成其突變之基因，以及觀察此性狀對遺傳表現所造成的影響。該計畫分成兩階段，第一階段的實驗重點是在突變株的搜尋以及純化上，第二階段則是在突變基因的探討上，由於特定突變株的搜尋並非容易，所以目前計畫只進展至第二階段的遺傳實驗初期，對於其遺傳特徵與突變形式上已有了初步的分析，但尚未定位出該基因的位置。

安非他命的濫用在台灣是非常嚴重的公眾健康及社會問題。安非他命會導致一連串的行\r 為異常，包括在中腦紋狀體內釋放多巴胺及阻止多巴胺回收來增加使用者的活動力。由於安\r 非他命會對腦細胞造成傷害，本研究的目的為探討低劑量、無立即毒性之安非他命(類似於人\r 類使用習慣)於短期內是否會對老鼠大腦紋狀體內的蛋白質表現有影響。因此利用西方點墨法\r 分析施打低劑量安非他命(2 到6 mg/kg)約一星期之後，C57BL6 小鼠的大腦紋狀體中一些重\r 要蛋白質[包括腺?酸受體A2A-R、第五亞型腺?酸環化?AC5、caspase-8、PARP、NF-κB\r 及血紅素加氧?-1(HO-1)]的表現是否有改變。實驗結果顯示，低劑量安非他命處理對大部分\r 蛋白質的表現並沒有明顯的差異，但在施打安非他命老鼠之大腦紋狀體中，HO-1 有些微但明\r 顯的增加，顯示安非他命可能對腦組織產生氧化性傷害。因此長期使用安非他命對中腦紋狀\r 體是否造成傷害是值得關心及繼續探討的課題。The wide spreading use of amphetamine (AMPH) in Taiwan has become a serious\r public health and social problem. AMPH evokes a series of behavior abnormality including\r enhanced locomotor behavior by releasing dopamine and inhibiting dopamine-uptake in the\r striatum. Since AMPH is known to cause brain damage, the purpose of this study is to\r investigate the expression of several important proteins in the mouse striatum after\r treatment with low and non-toxic dosages of AMPH for a short period (mimicking the\r common usage pattern of humans). C57BL6 mice were daily IP-injected with various doses\r of AMPH (0 to 6 mg/kg) for one week. Expression levels of adenosine receptor A2A-R,\r adenyl cyclase type 5,caspase-8, PARP, NF-κB and heme oxygenase-1 (HO-1) in the\r striatum were analyzed by Western blotting technique. Most proteins examined were not\r affected by the 1-week AMPH treatment, except HO-1. A slight but significant increase of\r HO-1 by AMPH treatment indicated that AMPH may cause oxidative damage in brain.\r These results suggest that the injury induced by long-term AMPH exposure warrants our\r further concerns and investigation.

k(2αβ ,α2 ? β2,α2 + β2 )是大家熟悉畢氏定理的通式解，且一般書籍的証明大都採用代數的手法證明。以國中生而言，上述的代數方對國中生來說不夠直接且較無推展的實用性。因此幾何觀點出發發展另一種思考方式，利用角平線的性質給予畢氏定理比例解另一種全新的詮釋，並賦予比例解中的參數α 、β 在幾何的意義。在推理的過程中，我們得到一個相當有用的對應關係：一個有理數對應到一個直角三角形、兩個有理數對應到海倫三角形，再將此對應關係運用到各種幾何圖形上面，即可證明出他們所對應的通式解。最後我的興趣鎖定在海倫三角形、完美海倫多邊形與超完美海倫多邊形上的做圖方法上，善用我們所發展的對應關係，上述的問題皆可迎刃而解。k(2αβ ,α2 ? β2,α2 + β2 ) is a popular formula in Pythagoras Theory, often proved in algebra approach among books. Nevertheless, in light of junior high students, the aforementioned algebra method is neither direct nor practical. Hence, a different thinking method is derived from geometry perspective, using the straight line concept to reinterpret Pythagoras Theory and define the geometric meanings of α andβ . In the process of logical development, a useful correlation emerges: a rational number correlates with a straight-angled triangle, and two rational numbers correlate with Heron Triangle. This correlation can be applied to all kinds of geometrical diagrams to prove the correlated homogenous solution. Ultimately, my interest lies in the diagram methods of Heron Triangle, Perfect Heron Polygon, and Super Perfect Heron Polygon in order to apply our developed correlations to solve the above mentioned problems.

植物合成澱粉?抑制劑可以對抗動物的取食，國外實驗證明數種澱粉?抑制劑對害蟲防 治具有顯著效果，其中以腰豆(Phaseolus vulgaris)研究最多。我們利用5% T.C.A.進行粗萃，從台灣常見豆類中篩選出四季豆(與腰豆同種不同品系)與菜豆，對麗蠅的澱粉?具有明顯的抑制效果，對豬胰臟與黃豆澱粉?的抑制效果則小或無，此種抑制特異性深具害蟲防治的潛力。經由溫度與pH 的試驗發現粗萃中的澱粉?抑制劑成分應為蛋白質。我們以四季豆作為繼續研究的對象，將粗萃進一步純化，經由陰離子交換與膠體過濾層析，分離出單一蛋白質，經蛋 白質定序比對確認其可能為國外發表的腰豆澱粉?抑制劑—αAI-1。經由測試發現此抑制劑在 85℃時仍能抑制果蠅澱粉?，為一相當穩定的蛋白質；且抑制劑的作用受pH 值影響很大，在偏酸性環境下的效果最好，與昆蟲分泌澱粉?的部位亦為酸性環境有相當密切的關聯；且其 抑制作用具特異性，可明顯抑制果蠅、入侵紅火蟻、白蟻、蟑螂及麵包蟲等昆蟲的澱粉?活性，對人類唾液、豬胰臟、四季豆本身及黃豆澱粉?的抑制效果很小或無，值得繼續深入研究。 Plant amylase inhibitors can fight against predation from plant-eating animals. It has been reported that several amylase inhibitors have an obvious effect on pest control; among them that from Phaseolus vulgaris got the most surveyed. 5% T.C.A was employed to make crude extracts. We have screened the amylase inhibitor activities from crude extract among beans common in Taiwan. The inhibitors from both string beans (the different strain of Phaseolus vulgaris) and cowpea notably inhibited the amylases in Chrysomia megacephala, but little or no inhibition in porcine pancreas and soy bean. This specific inhibition behavior suggested strong potential in pest control. Its activity can be affected by temperature and pH suggested that amylase inhibitors in crude extracts should be proteins. String beans were chosen to be further purified from the crude extracts. A single protein was isolated after ion exchange and gel filtration chromatography. Through protein sequencing, the partial amino acid sequences were highly homologous to that ofαAI-1 from Phaseolus vulgaris, indicating it might beαAI-1. The purified protein still can inhibit the amylase from Drosophila melanogaster at 85℃, suggesting it is thermal-stable. Its activity was affected by pH and reached the peak in weak acidic environment, which might be related to the fact that amylases are secreted in acidic environment of insect’s midgut. It obviously inhibited the amylases from D. melanogaster,Solenopsis invicta, Odontotermes formosanus, Periplaneta Americana Linnaeus, and Alphitobius sp., while not to human saliva, porcine pancreas, soy bean and string beans itself. The unique pattern of inhibition activities of the purified amylase inhibitor was worthy of further anlysis.

本實驗針對隔音材質的特性，如各孔板之孔徑、開孔率，並以夾層做不同孔板配置進行研究。本主題共進行三階段的實驗。第一階段探討不同材質的影響，因材質加工問題，實驗誤差過大；第二階段改以PP 塑膠板探討，因儀器誤差，數據難以解析；第三階段仍以PP 塑膠板探討，並修正實驗步驟，以降低實驗誤差。本報告乃以第三階段實驗結果做探討。為了提高實驗準確度，將整個實驗分成若干組，在實驗前後以空白測試，以減低實驗誤差。利用Excel 軟體，進行各組實驗數據噪音傳送損失之修正與分析。本試驗以125Hz、250Hz、500Hz、1000Hz、2000Hz 及4000Hz 六段週波頻率做測試。由分析結果顯示，孔徑與開孔率、同心圓的排列方式，對噪音傳送損失有顯著的影響。;This experiment is to focus on the properties of sound barriers, such as hole diameter, open areas, and the layout of holing boards. We have performed experiments in three steps. In the first step, we discussed the differences between different kinds of material but found many errors caused from faults in the process of making boards. So in the second step, we chose the ploy-propene board but still found errors from instruments. And in the final step, we used the ploy-propene board again and modified the experiment procedure to lower the error from instruments. The report is based on the final step data. With a view to raise the precision of the experiment, we divided the experiment into several parts with the blank test. We modified and transferred the original data to the noisy transfer loss (TL) in the same intensity level, and analyzed the data with Microsoft Excel. In experiment, we detected the noise-pollution level (dB) in the frequency of 125Hz, 250Hz, 500Hz, 1000Hz, 2000Hz and 4000Hz. According to the result, the hole diameter, open area and the layout of concentric circle have a significant influence on TL.

在酯化反應中，經由實驗結果，我們發現離子液體對於此反應有催化的效果。離子液體 是在室溫下呈現液態的離子化合物，將醇類與酸酐放入離子液體中有助於酯化反應的進行， 基於這個新的發現，我們開始尋找使用不同種類的離子液體做實驗，選出適當的離子液體， 並且測試離子液體在不同環境下的催化效果，以及適合的使用計量；更進一步，我們找出離 子液體在催化反應之後，將離子液體回收的方法：利用有機溶劑將離子液體和產物分層並萃 取出產物，把離子液體回收再利用，符合現代推動綠色化學的趨勢。接下來我們探討離子液 體對催化反應的擴展性與應用，先由不同結構的一級醇反應到醯胺鍵的生成，最後推展到合 成阿斯匹靈，實驗結果說明，用離子液體做催化劑，也可以成功的合成阿斯匹靈。 We have established for the first time that ionic liquids, which possess the property of Lewis acid, can facilitate acylation of alcohols with anhydrides to form esters with photo-excitation. With the initial finding, we then screen through different types of ionic liquids with varying counter anions, loading, and external light or heat sources to sort out the best reaction conditions. To gain insights into the working mechanism, the dynamic profile of the catalytic reaction was monitored by analyzing the reaction mixture by using ‘H NMR spectroscopy. The ionic liquids can be recovered by extractive separation from the acylation product, which meets the major theme of green chemistry. To extend the substrate scope and applications of the new catalytic process, different functional primary alcohols and amines were further examined. More importantly, we have utilized the new catalytic protocol for the acetylating of salicylic acid, leading to aspirin with high efficiency.

因為在我們高中所學到的細胞中，粒線體是提供細胞能量的重要胞器，所以細胞凋亡時，粒線體一定也參其中。 \r 本研究發現200μM過氧化氫(H2O2)作用6小時後存活率就低於50%，而誘發細胞死亡的過程中，我們發現細胞質內的粒線體有變的越來越稀疏情況，且集中在細胞核四周的粒線體也減少許多，其結構也變的鬆散不完整。最後在細胞核出現DNA被切成小片段時，粒線體的型態及結構完全消失，粒線體的量減少到極微量，這致使細胞完全沒有能量供給而死亡。 \r 結論告訴我們細胞的死亡跟粒線體的功能喪失及形態破壞有極大的關係。

本實驗主要是探究雙圈圈簡單飛機的飛行原理，歸納圈圈結構對飛行距離、升力的影響，以及氣流流經機體時發生的作用。研究結果如下：一、實際發射，歸納影響滑行距離的變因。1. 前後圈直徑比值約為0.8 時滑行距離為最大。2. 前後圈寬度比值越接近1 時，滑行距離越遠，但影響不大。3. 圈圈間隔在21cm 時，滑行距離最大。二、設置風洞，模擬飛機飛行，測量升力1. 圈圈寬度越大，升力越大。2. 升力最大值出現在圈圈仰角25 度左右，風速越快，升力越大。3. 鋁片仰角在20°時升力最大，升力與角度的關係式為 F = 5×10?7θ4 + 4×10?5θ3 ? 0.0083θ2 + 0.2615θ + 0.13744. 風速越快，升力越大，在仰角20°時升力與風速的關係大約為F = 0.4579V2 - 0.9231V +1.4772 。5. 鋁片面寬每增加1cm，升力也增加0.1513gw。前後長每增加1cm，升力即增加0.1263gw。三、設置蒸汽氣流，觀察簡單飛機的氣流場1. 蒸汽流通過圈圈時，會發生附壁現象，而且簡單飛機使氣流往下偏折，飛機得到升力。四、理論演繹︰1. 以康達效應的理論推算出升力，與實際測量得的升力約相等，驗證升力確實由康達理論造成。2. 墊高簡單飛機前圈，使得軸線提高，確實影響了飛行距離，墊高1cm 以內，飛行距離均增加了，以實際的改進證實升力確實是康達效應。This experiment mainly discusses the flying principle of the simple plane which is made up of a straw with two paper circles, one bigger than the other, stuck on both two ends of it. We first launched the simple plane actually and concluded the factors which influenced the sliding distance of the plane, including the distance between two circles, diameter and width of the two circles. Second, we set up a simple wind-tunnel and simulated the flight, in order to measure the strength of lift. Third, we set up the steam air flow and observed the change of the air current in the steam flow while flowing through the plane. The Phenomenon of Wall Enclosing happened and made the flows downward, and the plane gained the lift at the same time. Finally, we deduced that there are two sources of lift and Benoulli's law is not suitable for it. The Coanda Effect can be applied to figure out 54 percent of lift. And the current, blocked by the plane, also offers some lift. In order to prove that the Coanda Effect does effect, we padded the first circle to enlarge the angle of elevation of the axis of the two circles. It really affected the sliding distance of the plane. While the first circle is padded up within 1 cm, the sliding distance of the plane increases. Practical improvement proves that Coanda Effect accounts for the lift.