研究傳統降火氣食品是否具有抗發炎效果
在炎炎夏日總希望來點可以「降火氣」的食品,依據古籍記載某些中藥材具有降火氣的效應,而中醫所謂的降火氣與西醫的抗發炎反應密切相關。本實驗利用巨噬細胞株受到LPS (Lipopolysaccharide,革蘭氏陰性菌細胞壁表面所含有的脂多醣體)刺激後,會分泌發炎介質的現象,再分別加入各項傳統認為可降火氣(以菊花、薏仁、仙草作為樣品)或上火氣(選用龍眼作為樣品)的中藥食材,來評估中藥材對巨噬細胞分泌發炎介質能力的影響。經由實驗,我們發現市售的『降火氣』中藥食材,在適當的濃度下,確實具有降低IL-6、TNF-α 分泌量的能力,但濃度過高時卻會造成細胞凋亡。另外,傳統以水熬煮的方式比冷凍乾燥取得的樣品有更好的降低IL-6、TNF-α 分泌量的能力。 我們期望以此一系列的實驗,來建立篩選『降火氣』食材的抗發炎效用的研究模式,並在後續實驗中可用以篩選出更多具抗發炎效應的中藥材。In summer, people always consider eating some special things to refresh themselves. According to ancient books, there are some Chinese herbal medicines that were recorded to have the function of cooling us down, which is related to anti-inflammation in the Western. Macrophage cell lines can be stimulated by LPS (Lipopolysaccharide) and secrete proinflammatory mediators. Thus, it is often used to evaluate the anti-inflammatory effects of different herbs. In our experiment, we also use macrophage cell lines to test different anti-inflammatory effects between chrysanthemum, Job’s tears and grass jelly. In addition, some Chinese herbs such as longan that can parch us are also tested in our experiment. In our results, we found these herbs that are available indeed have ability to anti-inflame in appropriate consistency; however, high dose does harm to cells. In addition, the samples that are poached in ancient ways have better ability to show the low dose of IL-6 and TNF-α. Finally, we would set up a model to test the anti-inflammatory ability of different herbs that were said to be able to cool us down, and then we could test more herbs in the future.
魚洗水跳現象的探討
In the previous year study, we assumed that the fish basin as a column-shape model in order to exam and explain how the water spouting. This year, we established a reality-like situation of the fish basin to construct our theory base. At this point, round-waves and up-down waves are found as the beginning point of the water spouting. The sound from the basin is the frequency of the vibrations. Meanwhile, this process enables us to understand the causes of four, six, and eight waves. In addition, we tried to change the basin's positions, the capacities of water, the densities of liquids, and the sizes of the containers to demonstrate the theory. Furthermore, we used similar containers, such as high-heel cup and big magnetic bowl to experiment. The results are also corresponded with the theory. These results can also be used to explain the Chinese aeolian bell, clock, shaking bell, etc. That is a major innovation for us. Finally, we obtain better understanding of the abstractive theory though the simulation of the computerized photographs. 魚洗,也稱龍洗,傳說是中國唐宋時代皇帝洗臉的臉盆,內底四條魚紋,麟尾畢具。洗內盛水後,用手摩擦其兩個雙耳,立即發出響亮的嗡鳴聲,並出現美麗了漣漪,水珠四濺,摩擦越快,聲音越響,波浪翻騰,水珠越烈。對此在上一年度的研究中,是將洗盆視為圓柱狀,然後由圓柱狀所建立的模型去解釋水跳的原因,初步瞭解了水跳的原因。今年我們以洗盆真實的情況建立理論模型,在殼體產生共振的圓形駐波及上下駐波,此為水跳產生點,此時殼體發出的聲音就是共振的頻率。也瞭解了有四個、六個、八個、十個波腹的原因。並以改變殼體不同位置、水量、液體密度及洗盆大小等變因應證模型,結果與理論相符合。繼而以高腳杯、大磁碗等類似魚洗盆構造的容器,加以實驗應證,也能符合此理論。甚可推到中國古時候的編鐘、搖鈴、風鈴等器材。對於我們總認為碰撞就產生聲音的觀念實是一大突破。最後經由電腦動畫的模擬,讓此抽象的理論更容易瞭解。
誰能比我更善變???~ 釩鎵磷酸鹽的合成及其結構鑑定
This research tries to find out how changes of weight or proportion of a reactor would affect the type and structure of a crystal. In the experiment, the Hydrothermal reaction was adopted to conduct the syntheses of Vanadium-substituted Gallium Phosphates and try to pick up the single crystal to collect data with SXRD for further illustrating the crystal structure with diamond software to know well its nature. The research result finds out a new type of crystal unseen in the existing literature produced because of the weight changes of C3H10N2, therefore the weight change of a reactor may affect the structure of crystal significantly. In the future, it is expectation that comparison among structures of other Vanadium-Substituted Gallium Phosphates can be made, and the properties of this compound can be measured, and try to find out a brand new type of structure for new exploration by changing the initial reagent.
本研究係透過改變化合物所添加之反應物的比例,以了解反應物比例的改變對晶體的型態與結構的影響。實驗採用中溫水熱反應來從事金屬釩鎵磷酸鹽的合成。並挑取單晶,以X-ray 繞射儀進行數據收集,再以diamond 軟體繪出晶體的結構圖,且進一步得知其性質。研究結果發現,經改變丙二胺的量後,產生不同於文獻上的新化合物,可知改變所加反應物的比例,對於晶體結構有相當大的影響。未來可朝向與其他的釩鎵磷酸鹽做結構上的比較、對此化合物做更進一步的性質測量、改變起始試劑測試,進而得到更新穎的結構形式等部分進行探究。
化學中的數學與程式設計
When we were learning about organic compounds at school ,there was a unit discussing the isomers of alkane .Our teacher made us practice drawing all the structural formula of the isomers from hexane to nonane .We were much interested in the subject .However ,we often missed or duplicated some isomers .Thus , we began to think if it is possible to find a way by developing programs to let the computer calculate the exact number of the isomers of alkane . After discussion ,we set up a complete coding system .We numbered the isomers in the way that computers could decode and then wrote them in C language. Through computer execution ,the numbers of the isomers from C1 to C20 all match those on the reference website. According to the same concept , we also find a way to calculate the number of alkane with one substituted group . In the future,our goal will be focused on the research of multi- substituted alkane and cyclokane. In addition , the ionic crystal accumulation model are so variable. Take the double face-centered accumulation of NaCl for example, when the ion pairs are extended to the infinity , the potential energy of attractive field will approach a constant which is named as the Madelung Constant. We also managed to write a computer program with C language to approach this convergence with three models, including cube , octahedron , and sphere . The result turned out to be that the data of the sphere was less stable . In the other two models , when “n” is up to 43 layers , the data is identical with that on the reference website to the eight decimal point . 在學校裡學習有機化合物有關烷類異構物這個單元,老師讓我們練習畫出己烷~壬烷的所有異構物結構式,這引起我們極大的興趣!但常一不小心就漏掉或多出幾個,我們開始思考:能不能找到一個方法並設計成程式,讓電腦執行以找出烷類異構物?經過討論,我們建立了一套完整的編碼系統,將各異構物以電腦可解讀的方式編號,並以C 語言寫成程式。透過電腦執行,各碳數化合物自C1至C20都與參考網站吻合。依相同觀念,我們也設計出烷類含一個取代基的異構物數目。將來努力的目標為:多取代基及環烷類之研究!另外,離子晶體堆積模型變化多端,以NaCl 雙面心堆積為例,其引力場位能,當離子對延伸至無限大時,這個值將趨近於一個常數,又稱為馬德隆常數。我們嘗試以C 語言設計電腦程式,用三種模型(正立方體、正八面體、圓球)來逼近並求得這個收斂值。執行結果是:圓球數據較不穩定;而另二種模型到n=43 層以上,其數值大小與參考網路上的數值,在小數點以下8 位完全相同。
Molecular and Cellular Responses under Hypoxic Stress among Rice Cultivars with Different Flooding T
全球暖化造成水災頻繁,嚴重威脅植物生存。看似耐淹水的水稻,在完全淹水下亦有其生存危機。水稻FR13A 因耐水性極佳而常用於分子育種,IR64 產量高卻不耐水。是那些特質使稻種間有不同耐水機制?我們觀察其幼苗淹水24 小時後生長情形、通氣組織 (aerenchyma) 變化及應用即時反轉錄聚合?鏈反應 (real time RT-PCR),研究酒精醱酵的主要蛋白質:乙醇脫氫? (alcohol dehydrogenase, ADH1, 2) 及丙酮酸脫羧? (pyruvate decarboxylase, PDC2) 基因之表現量。兩種水稻的胚鞘及根都因淹水減緩生長,以FR13A 減緩最明顯。通氣組織在淹水期間都有增加,FR13A 中的形成近似於對照組,IR64 則明顯較差。FR13A 中ADH1 及ADH2 在淹水一小時後迅速增加60 至100 倍,IR64 僅增加10 至20 倍。PDC2 在IR64 中表現量的增加幅度較大,但最大值仍小於FR13A 之基礎表現量。由此可知,FR13A 在完全淹水時成長減緩而原有通氣組織則持續生長,且酒精醱酵中的基因有獨特誘導反應,因此耐水性較佳。藉由此研究揭開水稻細胞及分子生物學上的耐水反應策略,將可更精準地改良稻作使其對抗淹水逆境,解決未來因環境造成的糧食危機。Global warming increases the frequency of flooding, which drastically reduces the growth and survival of plants. Although rice (Oryza sativa) appears well-adapted to flooding of roots as it is often farmed in paddies, problems arise when the whole plant is submerged in water. I am interested in the structural and molecular responses that result in different submergence tolerances in rice cultivars. Indica rice FR13A is submergence-tolerant and frequently used in molecular breeding for this trait, while IR64 is a high-yield but submergence-intolerant cultivar. In this study, I monitored the growth rate, aerenchyma formation, and gene expressions of the carbohydrate metabolism in FR13A and IR64 seedlings subjected to submergence for 24 hours, by means of real time RT-PCR and microarray. FR13A had prominently inhibited coleoptile growth and sustained levels of aerenchyma formation whereas IR64 did not. The mRNA levels of alcohol dehydrogenase 1 (ADH1) in FR13A was induced prominently, while ADH2 was induced in IR64 during early hours of submergence. The induction of pyruvate decarboxylase 2 in FR13A was stronger than IR64. The expression of sucrose synthase was similar in both strains. Expressions of the genes involved in anaerobic carbohydrate metabolism were also studied by analyses of microarray data. My findings demonstrate that elongation quiescence, persistent aerenchyma formation and shifts in anaerobic carbohydrate metabolism gene expressions are beneficial strategies of FR13A towards submergence. Through elucidating the molecular basis of coordinating submergence tolerance genes as this study provided, it will be possible to discover multiple traits associated; hence crop improvement for flooding tolerance could be achieved.
一些Moire patterns 的數學性質研究
Moire 為法文,其英譯為watered, 是古代織布技術的一種應用;將印有規律條?的透明薄片重疊時,稍微移動或轉動其中的一片,會形成極大的圖形變化,稱為moire pattern本作品針對三個moire pattern 的數學式加以推導:(一)、兩張透明片各印有等間隔平行線,轉動其中一片使兩線的夾角θ,亮紋垂直距離和暗?垂直距離的比值為tanθ/2tanθ 。(二)、兩張透明片各印有輻射線,重疊後行成圓系,可由代數或幾何加以證明,利用三角函數可推導出此圓系方程式為:x2+{y-rtan[π/2-(θ-?)]}2)]}={rsec[π/2-(θ-?)]}2)]}\r \r (三)、透明片A 印有等間隔平行線,B 印有符合高斯曲線的平行線,AB 重疊時,形成一系列的高斯曲線,AB 的夾角減少時,會增大曲線的曲率,我們進一步討論曲線的曲率和平行線斜率的關係。Moire is the French word “watered” and refers to an ancient technique employed in cloth making. The moire occurs whenever two or more transparent sheets with periodic strips on them are superposed. The characteristic of moire patterns is the fact that a slight shift of sheets will create dramatic alternations in the observed patterns. In the present report, We derive the equations of three different moire patterns. First of all, take a sheet with equal spaced straight lines and placed it on top of another identical sheet. They are made to intersect and form an angle of θ. As the angle changes slightly, it produces huge changes in the spacing of moire fringes. We can derive a formula related to the interfringe distance. The ratio of bright fringes and dark fringes is tanθ/2tanθ.Secondly, two transparent sheets with radial lines on them are overlapped, forming a pattern similar to the lines of force between point charges. We can find that the pattern is a series of circle by means of algebraic and geometric proofs. And proven by trigonometric functions, we canconclude that they satisfy the equation :x2+{y-rtan[π/2-(θ-?)]}2)]}={rsec[π/2-(θ-?)]}2)]}\r Thirdly, a set of lines of equal spacing is overlapped with a second set of lines whose spacing are derived from a Gaussian curve. A series of Gaussian curves is reproduced in a moire pattern. Reducing the angle of intersection between the two figures steepen the curvature. We discussed the relation between the curvature and the slope of inclined lines.
聚球藻 RF 一 1 品系生物時鐘之特性
我們成功的用溶氣計偵測到了聚球藻 RF-1 在 28℃下光合作用的概日韻律。和傳統的研究方法比起來,這個方法具有連續偵測的優點,減少因不斷取樣所造成的影響,此實驗可觀察到聚球藻 RF-1 溶氣量之變化圖與一般藻類(如單殼縫藻)不同.在光 /暗條件下 RF-1 之溶氣量的增加與減少均呈週期性變化,而且此變化現象在進入連續照光後仍然可以維持兩個循環以上,這些結果顯示以溶氣計連續偵測聚球藻 RF -1之概日韻律是可行的,而且所得到的變化圖形遠比傳統方法(於不同時間取樣)所得者自然。本實驗同時發現含聚球藻之培養液的溫度,在進入黑暗週期時會有明顯的上升,由於其變化程度比其他藻類明顯,如加以探討應有助於對此藻以及其韻律特性之瞭解。We successfully detected the photosynthesis circadian rhythm of the prokaryote Synechococcus RF-1 under 28℃ by a DO (Dissolved-Oxygen) meter. The advantage of this method, comparing with the traditional methods, is that it can detect signals continuously, reduce the influence of discrete sampling. The DO curves of the Synechococcus RF-1 are different from that of other algae. Under Light/Dark conditions, the DO values of RF-i increased and decreased periodically. The periodic phenomena progressed over two cycles under constant lighting conditions. These results revealed the feasibility of using DO meter to continuous detect the circadian rhythm of the Synechococcus RF-1 The detected DO curves looked more natural than those obtained in the traditional discrete-sampling method. We also found that the temperature of the culture increased in dark cycle. Since the variation is clearer than that of other algae, further investigation will benefit the understanding of the Synechcoccus RF-i and its circadian rhythm.
敬"屑"不敏 --- 皮屑芽孢菌生長之探討
由於本身有頭皮屑的煩惱,在使用各種洗髮精之後,一方面覺得大部分的洗髮精效果不是很好,另一方面也擔心抗屑洗髮精的成分對人體有害。所以有了研究頭皮屑的念頭,希望能找出減少頭皮屑的方法。\r 在與幾位皮膚科專門醫師討論後,醫師們都不建議在直接人體上進行減少頭皮屑的實驗。於是我們將研究的重點放在〝皮屑芽孢菌生長之探討〞,希望對皮屑芽孢菌有更深入的瞭解。除了洗髮精之外,我們也將研究各種廣為流傳的民間偏方是否能有效抑制皮屑芽孢菌生長。在這些民間偏方的蒐集可兩大類:一類是在日常生活中可以很容易取得的物質,如鹽、醋、大蒜、生薑、洋蔥等;另一類則是台灣特有的物質,如無患子、茶籽粉、竹醋液等。\r 我們希望透過研究方法能瞭解\r (一) 溫度、濕度、酸鹼度、油脂對皮屑芽孢菌生長的影響\r (二) 哪些物質可以抑制皮屑芽孢菌的生長\r (三) 不同稀釋濃度的物質抑制皮屑芽孢菌的生長的效果\r 為了避免繁複的數量計算(如菌數計算),我們採用較多的對照比較(如抑制圈大小)。同時,我們設計了一個簡易的觀察與測量方法(稀釋法與抑菌指數),來比較各種民間偏方\r 的抑菌效果。\r 研究與實驗分為四大項進行:\r (一) 基本生長環境之探討:溫度、濕度、酸鹼度、油脂對皮屑芽孢菌生長的影響\r (二) 民間偏方抑制效果之探討:鹽、醋、大蒜、生薑、洋蔥是否能抑制抑制皮屑芽孢菌的生長\r (三) 民間偏方抑制效果之比較(抑制圈):比較各種物質的抑制圈大小\r (四) 民間偏方抑制效果之比較(稀釋法):利用抑菌指數來比較各種物質的抑菌能力\r 經過研究,我們所蒐集到的民間偏方都有某種程度的抑菌效果,大致可以分為強效(蒜液、竹醋液、茶樹精油)、有效(食鹽水、食用醋、洋蔥液、檜木芬多精、茶籽粉)及普通(薑汁、無患子)三種效果。\r \r Since I have the serious dandruff problem, I try to seek, except the shampoo, the natural materials to decrease the dandruff. After consulting with some dermatologists, they suggest I can focus my research on the “Malassezia Furfur”, which is the major bacteria causing the dandruff.\r This project is designed for finding the materials which have the most powerful inhibition capability for the “Malassezia Furfur”. I collect some folk prescriptions from the internet that salt, vinegar, garlic and onion can decrease the dandruff. The problem is “Do they have good inhibition capability for the Malassezia Furfur too?”\r The goals of the research are as followed.\r (1) The exploration of the basic environmental factors for the growth of Malassezia Furfur, such as temperature, humidity, acidity & alkalinity.\r (2) What kinds of material have the good inhibition capability?\r (3) The comparison of inhibition capability of the different material with different consistency.\r We especially design a method called “Dilution metering and index” to compare the inhibition capability of the different material. By using very simple observation, this method will transfer the simple “Qualitative Analysis” to the result of complicated “Quantitative Analysis”. The “Dilution metering and index” is the major achievement of this project, and it is also the good way for the projects which are not equipped with the precision instrument for bacteria inhibition capability measurement.