全國中小學科展

一等獎

果蠅單眼的發育調控基因之篩選

在果蠅成蟲的頭頂上方三顆可以感光的單眼(ocelli),其構造和複眼有顯著的差異,但單眼及複眼在果蠅幼蟲時期,皆從一片特別的組織eye-antenna disc發育而來。而且目前已知會表現在單眼的一些特定基因,也是會調控複眼發育的基因,如sine oculis (so),eyes absent (eya) 等。但到底是哪些特定的基因決定了單眼與複眼的差異,目前並不是很清楚,因此我想探討果蠅單眼細胞在發育過程中,有哪些特定的基因會調控果蠅單眼的生成與變化。 本實驗主要是利用果蠅研究上常使用的基因表現系統(UAS-GAL4 system)進行研究,我們挑選許多和複眼發育相關的基因、胚胎生長發育相關基因進行表現,觀察其單眼性狀有無變化,篩選出會影響單眼發育的相關基因後,再進一步研究此基因的可能作用機制。 從實驗的結果中發現當表現ras及Egfr的顯性抑制型 (rasDN, EgfrDN)時,在掃描式電子顯微鏡下可觀察到三顆單眼會明顯變小。而在幼蟲時期的eye-antenna disc,利用免疫螢光染色以及雷射共軛焦顯微鏡觀察到當表現rasDN、EgfrDN時, 單眼標記基因(marker gene) Eyes absent (Eya)表現的強度顯著降低。再加上已知ras為Egfr的下游基因,綜合這些結果,我們認為Egfr-Ras 以及其下游基因的訊號傳遞對於單眼發育是很重要的。 另外在視網膜電流圖(electroretinogram)的實驗當中,發現Egfr, ras訊息傳遞被抑制的果蠅單眼在對光線刺激的反應強度比起正常的果蠅下降許多,因此可推論Egfr, ras的訊息傳遞不僅會影響單眼的發育,也會影響單眼的正常感光的功能。

倍位元灰度影像產生器

本研究設計一新型的影像投射系統,可將影像顯示的灰度位元加倍,例如,顯示面板只需用4位元,即可顯示8位元的影像;亦能充分利用光路光源,增加光源使用率。此系統使用兩片相同灰度位元的顯示面板,此兩面板所顯示的影像經過灰度的重新處理,且各經由不同光源強度比值的光路合成後,其灰度分佈將可增為原來的平方倍。經模擬與實驗顯示,此種系統很輕易就能獲得預期目標。無論使用穿透式或反射式皆可應用於目前單片液晶面板之投影系統中;未來可望利用網板來表現灰度,應用於紅外線景物投射系統中,作為紅外線影像式尋標器靜態模擬時所需的高強度動態範圍與高解析度之影像產生器。In this study, a novel image generator utilized in a projecting system has been proposed; it can double the bits of gray-level for image display and enhance the efficiency of illumination of lamp in the optical path. With this system, a 4-bit display panel can achieve an 8-bit image display. Two display panels with same gray-level bits is adopted, images on them will be processed, and then go through different path with a proper intensity ratio. The gray level distribution of image displayed which the two images combined afterward, will be the square of that of original one. The results of simulations and experiments have approved to meet the requirements. No matter transmitting or reflective types can be applied to current projecting systems with single LCD panel. It is expected that a halftone-gray-level pattern will be suitable for this system to form an infrared scene projector, and to act as an image generator with high dynamic range and resolution for static simulation of infrared imaging seeker.

對平面結構的石墨烯修飾並應用於直接甲醇燃料電池

此研究希望找到適當的材料和製備方式,提升直接甲醇燃料電池 (DMFC)的工 作效能。 使用石墨烯為基材,將純度99.999%的石墨在高溫下以過錳酸鉀進行氧化,經 剝離後得片狀級氧化石墨烯(GO),然後以氯鉑酸(H2PtCl6)提供正四價的鉑(Pt),還原 在此樣品上成零價鉑金屬做為催化劑的使用。使此樣品(G-Pt)於三電極系統下進行循 環伏安法,分別對硫酸與甲醇進行催化量測此新型材料的氧化電位。對硫酸進行催 化,可得氫的脫附面積;對甲醇進行催化,則是觀察此新型材料的運用成效之優劣。 本實驗進一步於氧化石墨烯(GO)加入苯甲醯氯(Benzoyl chloride)做修飾,合成經 氧化修飾的石墨烯(GO-B),同樣地還原觸媒鉑在此材料上(G-B-Pt)。經電化學分析 後比較是否因修飾後接上苯甲醯氯而有比較好的性能表現。 結果發現,經苯甲醯氯修飾的材料,氫的脫附及甲醇的氧化電位數值表現上高 於未修飾者,說明在三電極系統下,本實驗加入的修飾藥品有助於提升直接甲醇燃 料電池的工作效能,將來可被運用於燃料電池上。

將錯就錯的knuth 河內塔

在這篇報告中,我們探索了「將錯就錯的Knuth 河內塔問題」。傳統河內塔問題在電腦科學上佔有重要的地位,是一個極具內涵的模型。由於這個模型的深厚數學內涵,使其和巴斯卡三角形建立了緊密的連結,且利用這個緊密的數學連結,設計出復原任意起始狀態的良好演算法。Knuth 河內塔起因於數學家Knuth 在論文[3]中,描述傳統的河內塔問題時所發生的一次筆誤。在這個新的規則之下,我們意外發現Knuth 河內塔存在著一個和傳統河內塔平行的模型,此模型在電腦科學及數學上有著完全不同於傳統河內塔的內涵。我們的研究主要如下:(分別為內文中的四大段)(一) 結構分析。移動環所需要的次數,如何移動環並分析每一次動作所動的環,及每個環何時被動到並給出演算法。(二) 正整數的分割。所有的移動步驟將正整數做了一個新的分割(Partition);此分割模k之後有良好的循環性質。(三) 費波那契真分數的排序。這個正整數的分割形成一張表,這張表恰好就是分子分母皆為費波那契真分數之排序。(四) 隨意亂排的Knuth 河內塔復原演算法。在Knuth 河內塔的規定下將起始狀態改變,找出良好的復原演算法,並分析。 In this project we study the "Knuth Hanoi Tower", which is motivated by a typo in a paper of Knuth. This inadvertently typo leads to a new rule of moving the discs on the Hanoi Tower (see introduction below for definition). Although seemingly similar to the traditional Hanoi-Tower problem, it turns out that under this rule the "Knuth Hanoi Tower" problem consists of amazing properties, and is totally different from the traditional one. Our study focuses on the following directions: (1) Structure analyzing: We analysis the sequences recording the disc moving and offer enumeration results and recurrsive/non-recurrsive algorithms. (2) Partition of N: The moving sequence forms a partition (a table) of N, which has an amazing congruence property. (3) The order of Fibonacci proper fraction: The row/column of the partition table is, even more amazing, exactly the order when sorting the Fibonacci proper fraction with fixed denominator/numerator. (4) The Restoration of an arbitrary initial state: We offer an efficient algorithm for restoring any initial state of discs. We hope that our study on the "Knuth Hanoi Tower" offers a simple, neat, and new example on the theory of Algorithm, Number theory and Combinatorics.

植物miRNA的特殊長度─生成與演化

基因靜默為各種生物普遍擁有的調控機制,是以microRNA (miRNA)和Dicer-Like (DCL)與Argonaute (AGO)等數個蛋白質來完成。在植物,miRNA大多是由DCL1截切前驅物所產生,因DCL1具有21-nt 分子尺,所以絕大多數miRNA的長度為21nt。然而,其他特殊長度卻也有一定比例的存在,且可能有特殊的功能。最新研究發現22nt的miRNA可誘發更多的小片段干擾核酸來造成大量而強烈的基因靜默;然而,一般21nt的 miRNA卻無此功能。本研究以生物資訊的方法分析大量資料庫,並以分子生物學實驗佐證,探討特殊長度miRNA的生成、功能與演化。結果發現,20nt的miRNA可藉由對稱性與非對稱性縮短產生,這是RNA結構研究的新發現。功能上,20nt的miRNA與生長發育有關,22nt的miRNA則多與逆境反應有關;在演化上,長度20nt的miRNA呈現高保守性,而大部分22nt的miRNA則呈現高度物種專一性,為演化快速的特殊長度。

半屏山之簷下姬鬼蛛的研究

The spiders, Neoscona nautica, often appear in groups, but individuals have their own sense of territory.They usually spin webs among branches during 6:00~ 8:00 in the evening. When building webs, they will first start with bridges and then spin Y-shaped spokes. Next, they spin meshe of net, silk frame, spokes, spirals and free-zone in order. After finishing webs, they will wait for prey on the free-zone or meshe of net. If they find something inanimate on the web, they will break the spiral attached with the inanimate object that is later removed. If the meshe of net is broken, they will fix it immediately. For them, the time to take webs back is during 2:30~ 6:00 in the morning. Most time they use the first pair and the second pair of legs to take webs back and swallow the webs. Sometimes, they break the spirals by the last pair of legs. The sequence to take webs back is : lower right section, lower middle section, lower left section, upper left section, and upper right section. At last, one thread of bridge will be left. Every early mornings they take webs back and swallow them. The next evening they rebuild webs. Possibly there are two reasons to explain why spiders eat their webs: (1).They swallow webs to get protein. (2).The web threads are easily polluted by dust and humidity and reduce stickiness. The web may also reduce the probability of capturing prey. The body length of them is not related to effective web dimensions. However, the web sizes depend on the width of web-building location. The study shows linear relation among body length, meshe of net and dimensions of free-zone. The linear relation represents that the meshe of net and free-zone have ecological or survival meaning for them. We expect that this study of Neoscona nautica can be helpful to build spider ecological database in Taiwan.簷下姬鬼蛛常成群出現,但個體卻有很強的領域性;常於下午6:00 至8:00 結網於樹枝間,結網時,先以橋絲為出發,織出一Y 形的縱絲,再由此依序織出中空網眼、絲框、縱絲、橫絲、棲息圈,網結好後,簷下姬鬼蛛則在棲息圈或網眼靜候獵物,若發現網上有非生物之異物,則將黏住異物的橫絲弄斷,再把網上的異物丟棄;若網眼被破壞,則會立即修補。收網時間為凌晨2:30 至凌晨6:00,收網時,大部分由第一、二對步足進行收網,偶爾會用最後一對步足將橫絲弄斷,一邊收網一邊將網吞食,收網的順序為:右下、中下、左下、左上、右上,最後留下一條橋絲。簷下姬鬼蛛每天清晨都會收網,並將網吃掉,翌日傍晚再重新結網,其可能原因有兩點:(1)將網吃掉以補充蛋白質。(2)蛛絲容易受灰塵、水氣之污染而減小黏性,降低獵捕功效。簷下姬鬼蛛體長與有效網面積無關,但網的大小視其結網地點寬敞程度而定。體長與網眼、棲息圈面積呈線性關係,表示網眼和棲息圈對簷下姬鬼蛛具有生態或生存意義。我們對簷下姬鬼蛛生態調查之結果,希望能幫助台灣的蜘蛛生態資料庫之建立。

音材施教-音高與音色辨識之探討

我們使用C++撰寫了一個音準練習程式:使用者輸入聲音後,經由音頻辨識方法求出其頻譜中最高能量之頻率,以之為基頻,再將其與目標音高相較,得到誤差率及走音程度。此外程式還可秀出所唱的樂譜,和發出對應的鋼琴或正弦波的聲音,方便使用者校音。而為了做音高辨識,我們也收集了許多聲音檔案,觀察其特色,研究不同音色的頻譜或是波形特性,並利用其特徵完成一個音色自動辨識程式。\r 首先我們測試了各種演算法,並且選用了快速傅利葉轉換作為主要製作的演算方法;接著我們利用Microsoft Visual C++撰寫我們的程式。這個程式主要可分為錄音、辨識以及樂譜繪製三大部分,皆會在此份報告中詳細說明。\r 文中將說明音頻及音色辨識的方法,一些關於音樂的基本知識,微軟公司的wave檔格式,此系統之應用,以及音色的波形頻譜分析。我們使用FFT 求得聲音的頻譜,且將針對此部分演算法做簡單的說明,並探討如何達到所需之頻率準確度,如何以較高效率辨識,及如何找出不同音色的特性。

外觀數列

The Look and Say sequence is produced by describing the appearance of the previous row. For example, start with “1,” which can be described as “one 1,” and therefore the second row is “11,” which is "two 1s," making the third row “21,” the fourth row “1211,”and so on. The main goal of this study is to work out the exact formula for this sequence, which means given the row number n, we can know at once what the n-th row is without having to start from the first row and doing the look-and-say iteration for n-1 times. Some of the methods used include dividing groups, repetition and cracks. The formula we derived speeds up the calculation and gives us a better understanding of the look and say sequence.「外觀數列」為依照外觀產生下一列的數列,第一列為「1」,第二列描述第一列「1 個1」而為「11」,第三列則描述第二列「2 個1」而為「21」,第四列「1211」,依此類推。本研究針對外觀數列的各項數學性質作研究探討,並由此推導出外觀數列的一般式,即給定第n 列就可知道該列的內容。我們運用了分組、重複性以及裂縫的方法分析數列,最後得到了其一般式,此一般式有助於運算速度的加快以及我們對數列性質的了解。

聚對苯二甲酸乙二醇與聚乳酸共混材料之降解研究

本實驗目的在於嘗試降解傳統塑膠材料。實驗中選用極為常見的傳統塑膠,聚對苯二甲酸乙二醇ploy ethylene terephthalate (PET),與生物可分解材料,聚乳酸poly lactic acid (PLA),將兩者以不同比例物理性混摻,並以豬胰線脂肪酵素Lipase from porcine pancreas, TypeⅡ (PPL)進行降解,期望藉由生分解材料可被完全分解的特性牽引傳統塑膠材料進行降解。\r 本實驗共分兩階段:第一階段實驗中,將共溶劑揮發製膜進行降解,但由於溶劑法所留下的孔洞,影響了降解速率;因此,在第二階段實驗中,改以熱壓的方式製膜解決孔洞問題,並於降解後以儀器分析降解情形。結果發現:混合薄膜經降解後熔點有下降的趨勢,此可佐證確實可以生分解材料PLA牽引傳統塑膠料PET進行降解,且降解速率和結晶情形和分子鏈段纏繞程度有關,在PET與PLA濃度同為3%時降解情形最佳。

Wind Power

My school requires year 13 students to complete a year long project of a topic of their choice, culminating in the presentation of a thesis, a display and speech to a public audience. Many different topics appealed, but in the end I decided to build a micro wind-turbine. I have always been fascinated with mechanics, mathematics, engineering, aerodynamics and electricity. A wind turbine is a mixture of these technologies, with the overall goal of electricity production. In a world that is starting to see the true costs of fossil fuels, renewable energy seems to be increasingly popular and the demand for electricity is always growing.\r I was aware that building a wind-turbine from the foundations up wouldn’t be easy. Many of the experts I contacted in the early days cautioned me against trying such a complex thing in one year, at the same time as completing a full Year 13 course. There were, however, people prepared to support me. Michael Lawley, who builds micro wind turbines in New Plymouth was very helpful, just full of priceless knowledge and gave me a few basic parts to start with. The knowledge gained from Doug Clark, who also builds his own 11 kW wind turbines, was such an inspiration. Later I had practical help from Wilson Springford and Darron Matthews.\r I investigated and documented the history of and current state of wind technology, as well as my own experience and learning in the design, construction and testing process. I thought it would be interesting to find out how the electrical and mechanical side works.\r The generator, a washing machine motor, needed to be completely rewired, and converted to DC (direct current) from AC (alternating current). I built my own 3-phase AC to DC converter.\r Other parts like the disc brakes and bearings had to be found. The rest was hand-made and every part, to a certain extent, had to be modified. Probably more than twenty braking system design attempts led to the final decision to incorporate the wind-activated hydraulic disc brake where the wind paddle starts to ease the brake on over a certain wind speed.\r I studied the dynamics of wind turbine blades, their shape, the material they were made from and how this affected their performance. The decision to make my own blades helped me gain a great sense of achievement and knowledge of blade design. I found some New Zealand made 100% recycled plastic pipe, an added bonus because I wanted to have minimal environmental impact. I designed the turbine with three blades to give better starting torque along with a lower top speed, perfect for how I wired the generator.\r I designed the swivel, the part of the wind turbine that enables the power cables to get from the turbine down the tower without twisting up and has the job of carrying the whole turbine, which is mostly made from recycled aluminium. The steel and bearings used to create the swivel were all second-hand parts and materials. The power from the turbine passes through the swivel into the cables and down the tower. The main mast of the tower is a little over 4.7 metres and pivots on two shorter supporting poles which go down around 2.6 metres to the bottom of the reinforced concrete foundation.\r I managed to, design and construct an operational prototype micro wind-turbine, incorporating recycled and recyclable materials as much as possible, with the end result surpassing all expectations.