全國中小學科展

一等獎

南太平洋在北半球冰川擴張事件時物理海洋與碳埋藏速率變化研究

北半球冰川擴張是過去三百萬年來全球主要的氣候變遷事件之一,地球從溫暖的上新世轉變為較冷的更新世。本研究利用國際大洋發現計畫位於南太平洋 U1541 號岩芯中的有孔蟲群落分析和沉積物總碳變化,探討海水物理性質及碳埋藏速率變化受到此事件的影響。本研究發現在 2.8-2.5 百萬年前(Ma) 海水層化現象較強,2.5-2.4 Ma 受到北半球冰川擴張事件影響,海冰擴張,Neogloboquadrina pachyderma 相對豐度逐漸上升。冰期時南大洋海冰擴張後,導致換氣率下降,使得有機碳保存度高,讓總有機碳含量增加,此機制亦可能使冰期時南大洋深部碳儲藏庫開始擴張,使得全球冰川體積在第四紀時持續增長。本研究發現首次證實南大洋在北半球冰川擴張事件中扮演可能的二氧化碳儲存庫。

利用氧化應激法結合膠原蛋白微島嶼誘導人類臍帶間質幹細胞分化

再生醫學透過控制幹細胞分化以應用於修復受損或是建構新組織。目前誘導幹細胞分化的方法多為添加成長因子的化學誘導法,然而此方法具有高成本和費時等缺點。在本篇研究中,我們以人類臍帶間質幹細胞,作為誘導分化對象,使其貼附於具最適化圖形的膠原蛋白微島嶼,結合大氣電漿及 H2O2 二種氧化應激法刺激細胞,探討誘導 WJ-MSC 幹細胞分化的最佳條件。在本研究中,我們發現單獨使用大氣電漿處理可使成骨分化率增加,而大氣電漿處理再加上星形微島嶼培養細胞時,則更顯著的提高成骨分化率。未來嘗試以 H2O2 誘導幹細胞分化, 並進一步探討大氣電漿和 H2O2 提高脂肪分化率的條件、影響分化的機制,期望能此新技術取代傳統化學誘導法,對組織工程做出貢獻。

以廢棄香灰與陶瓷3D列印製成磚瓦於改善環境酸雨與碳捕捉應用功效之研究The Study of the Utilization of Waste Ash for Ceramic 3D Printing Bricks to Improve Acid Rain Issues and Carbon Capture Applications

廢棄香灰處理不易且對環境具危害經定性分析知有 47.74% 鹼性物質 CaO。本研究以廢棄香灰製成屋瓦及地磚,探討與酸雨酸鹼中和之可行性,經歷一年多且進行多次屋瓦逕流及浸泡酸雨實驗,觀察到香灰試片與酸雨中和效率和內含香灰比例呈現正相關,結果顯示摻入5%~20%香灰所製成的屋瓦的酸鹼中和能力皆適合臺灣環境使用且經一年放置於自然環境實驗仍具有良好的酸雨中和能力;若欲同時具有酸雨酸鹼中和與碳捕捉能力,則以含 10%~20%香灰為佳。摻入超過 20%香灰製品則降低陶製品之黏性導致無法成型。香灰製品相較於傳統屋瓦有製程價格低、高耐用性和酸鹼中和效率高等特性,可作為屋瓦材料或地磚的新發展。製作地磚部分因水泥會包覆香灰陶粒,因此採孔洞較大的陶瓷摻雜香灰進行 3D 列印,可快速製作不同環境應用之地磚,亦可同時具有碳捕捉與酸雨酸鹼中和之功效。

惡意程式無所遁形—以自然語言處理模型實現惡意程式之識別

本研究旨在運用自然語言處理技術,建立辨識惡意程式的模型。首先在資料集方面,分為 PE資料集與 ELF資料集,均包含良性及惡意執行檔,且蒐集惡意程式時採用多種不同之惡意程式家族。接著對資料集進行反組譯及前處理。使用反組譯後的組合語言檔作為文本,訓練模型以區分良性和惡意程式。研究比較詞袋模型、序列模型、BERT 以及不同 n-gram對模型的影響。 研究結果顯示。詞袋模型以使用multi-hot編碼表現最佳,PE資料集之F1-score為96.87%;序列模型則是有位置編碼的 Transformerencoder 表現最優。在不同 n-gram 的比較,multi-hot詞袋模型與 TF-IDF 詞袋模型,分別在 2-gram 及 5-gram 有最高的 F1-score。

探討一種新穎腺苷調控藥物對於思覺失調症及焦慮症的治療潛力-以藥物及壓力引發之疾病小鼠為模式 Investigating the therapeutic potential of a novel adenosine modulator(NAM) on the treatment of schizophrenia and anxiety disorders using mice as a model

思覺失調症與焦慮症皆是嚴重損害病人健康且造成社會極大負擔的精神疾病,急需研發有效治療藥物。本研究以藥物及壓力引發之小鼠異常行為為模式,探討新研發的新穎腺苷調控藥物(Novel Adenosine Modulator)對於這些疾病的治療潛力。實驗一以藥物 MK-801 引發成年雄性小鼠類思覺失調症症狀,給予新穎腺苷調控藥物可緩解曠野測驗中 MK-801 引發的過度活動量,且不會造成小鼠產生失樂症狀或體重改變。但對於 MK-801 引發的感覺動作門閾過濾反應缺損則無顯著療效。實驗二以不可預測長期輕微壓力(unpredictable chronic mild stress)引發成年雄性小鼠類焦慮症與類憂鬱之行為異常,新穎腺苷調控藥物可以改善小鼠的失樂症狀。這些結果顯示這個新藥具有潛力值得後續繼續研究。

開發組織蛋白酶B之抑制劑前驅藥物及其效能探究

在某些癌症或癌前病變時,存在於溶酶體的組織蛋白酶 B的表現量會增加,目前雖然已有Ac-LVK-CHO藥物可有效抑制組織蛋白酶 B,然而此藥物無法充分進入溶酶體,精準發揮藥效作用。本研究透過自行合成 Ac-LVK-CHO,且在其結構上進行化學修飾,製得前驅藥物以提升藥物效能。此研究自行設計合成方法與修飾基團,已成功在Ac-LVK-CHO 的胺基修飾上引導基團 morpholine,使其更易進入溶酶體,同時也在醛基修飾上光解性保護基團 2- nitrobenzyl alcohol,使此前驅藥物在溶酶體內可經調控發揮活性。實驗已驗證前驅藥物的光解性質,以HPLC 圖譜與質譜分析確認此藥物照光 30 秒可完全被釋放,且光解過程為一級反應。後續將統整前驅藥物富集於溶酶體的定量結果,完成此合成前驅藥物的全面性效能評估。

New Concept of Intelligent Wound Dressing

Substance losses, burns and injuries arising from various causes represent a constant problem encountered by homo sapiens throughout its existence. Rudimentary treatments, relatively effective and less effective, have left their mark on the way we perceive the presence and treatment of wounds caused by various factors. Searching through medical archives, we can trace the specific protocols for these medical conditions back to 2200 BC, when they were structured in three steps: 1) cleaning the skin lesion, 2) applying a dressing (from glue to various preparations) and 3 ) bandaging the skin lesion. Currently, the appearance of wounds on the skin is caused both by accidents in the performance of various activities and by certain diseases that manifest themselves through skin rashes or skin lesions. Their frequency is in the thousands, according to the latest statistics, affecting the majority of the population non-selectively. It is vital that skin lesions receive the necessary care and attention, commensurate with their severity. Being open wounds on the surface of the skin, it is essential that the treatment be meticulous and appropriate to their type, as skin lesions represent a threat to the patient's life. From infections to hydroelectrolytic imbalances specific to burns, the multitude of factors that influence healing highlight the need for a dressing that can be easily customized according to the specificity of the wound, the needs of the patient and that is affordable both from the point of view of production cost as well as its use, making death from skin lesions easily avoidable through an intelligent approach. One of the most complex biological processes and indispensable to humans is the healing of skin lesions. Healing involves a carefully regulated series of biochemical and cellular activities in tandem. Traditional therapies and substances of natural origin have been used to facilitate the regeneration process and accelerate the wound healing process, being applied with encouraging results. Despite the fact that these generally present a low cost, they can be more expensive than contemporary treatments and can be influenced by regional, seasonal factors, showing fluctuations from batch to batch, which could lead to unpredictable allergic reactions, side effects and inconsistent clinical findings. Currently, the standard of care for skin lesions is to clean the wound with antiseptic solutions to prevent infection, apply a dressing followed by bandaging to keep the dressing in place, and if necessary excision of the tissue that has become non-viable. In the case of diabetic ulcers, it is necessary to excise the tissue that has become non-viable and to maintain control over the level of glucose in the body.

Anti-forma Chitogel

Formaldehyde is an air-borne, carcinogenic indoor pollutant. It may cause adverse effects on human health such as irritation of eyes and respiratory system. Shells of hermetia illucens, Black Soldier Flies (BSF) are leftovers when the insects mature from pupae to adults. BSF shells are rich in chitin which can be converted into chitosan by demineralisation and deacetylation. Chitosan and its ammonium salt (chitogel) can remove formaldehyde via condensation of water. In this investigation, the efficiency of removal of formaldehyde by different substrates were compared including shells of BSF before and after demineralization, deacetylation and action of vinegar; and common commercial products and Anti-Forma Chitogels made from shells of BSF and some crustaceans. Anti-Forma Chitogel of BSF was found to be effective in removing (91.2%) formaldehyde (1:20 by mass) among shells of BSF with different treatments and its efficiency was better than all commercial products tested. Concentration of formaldehyde in the container with deacetylated Anti-Forma Chitogel is 0.54 mg/m3. It removed 74.8% of formaldehyde compared to the control (2.14 mg/m3). Concentration of formaldehyde in the container with Anti-Forma Chitogel without deacetylation is 0.76 mg/m3 . It removed 64.5% of formaldehyde compared to the control (2.14 mg/m3). The Anti-forma Chitogel of BSF was found to be eco-friendly with high formaldehyde removal efficiency when placed in a drawer (removal of 54.8% of in 24 hours), the chamber of a newly renovated room (removal of 84.9% in 30 minutes reducing the conc. of formaldehyde from 0.53 mg/m3 to 0.08 mg/m3; cf. the safety limit of formaldehyde <0.125mg/m3) and drawers of a new wardrobe (removal of 83.7% at 20.2oC in 1 day reducing the conc. of formaldehyde from 0.49 mg/m3 to 0.08 mg/m3 & kept the conc. of drawers below 0.125mg/m3 most of the time over a month when temperature was below 21oC). Conc. of formaldehyde in air-tight boxes (5g of construction adhesive in 9.3 dm3) with air purifiers with and without Anti-forma Chitogel as filter before and after 3 hours was reduced by 44.5% (from 6.25mg/m3 to 3.47mg/m3 ) and 27.7% respectively showing that Anti-forma Chitogel as filter in air purifier outperformed that without by 160%. Besides, anti-forma Chitogel is antibacterial, so it would also kill bacteria when used in air purifiers. [1] proving that Anti-forma Chitogel is effective in removal of formaldehyde on the spot and can be applied to households. It can also help achieve Target 3.9 and 12.5 of the Sustainable Development Goals of the United Nations.

Vitas: Digital Therapy Based on a Combination of Binaural Beats and Autonomous Sensory Meridian Response (ASMR) to Reduce Stress in Gen Z Students

Vitas is a combination of binaural beats (BB) with background white noise (ASMR) as accompanying music without reducing the quality of brain stimulation and is able to stimulate the left and right brain while synchronizing brain waves so that it can be used as a media for coping with stress for Gen Z students. To find out the effect Vitas in reducing stress levels, research was conducted on high school students belonging to Gen Z. This research aims to measure the stress index of students belonging to Gen Z, then find the effect of the combination of binaural beats (BB) and ASMR on students'stress levels, and finding the best audio combination to reduce stress levels as a coping mechanism. This research uses a quantitative approach with a quasi-experimental design using a one group pre-test-post-test design scheme. The sample in this study consisted of 105 students who were divided into seven treatment groups to listen to a combination of BB and ASMR with different bandwidths. Stress levels were measured using the DASS 42 stress section questionnaire which has been tested for reliability and validity. The results of the student stress index were 16.26 in the mild stress category. There was a significant difference between the negative group, the alpha + ASMR group and the positive group with a T-Paired test result of less than 0.05. Based on the results of the Post Hoc LSD test, it was found that the combination of binaural beats alpha (12hz) + ASMR was the best combination for reducing stress in students.These findings were developed into a digital therapy application product called "Vitas" as an alternative media for coping with stress for Gen Z.

Resolving the Phosphate Fertilizer Dilemma through Progressive Wastewater Treatment

The study aimed to resolve global phosphate scarcity by developing a cost-effective method for phosphorus recovery from industrial wastewater. In existing wastewater treatment, oxidizing phosphite ions (PHO32-) posed a significant challenge. However, our research aimed to develop an effective method for this oxidation process, crucial for phosphorus recovery in industrial wastewater treatment. By utilizing low-cost iron compounds and innovative catalysts, such as iodine obtained from seawater and copper, we achieved remarkable success. Our method demonstrated the ability to oxidize over 80% of PHO32- into phosphate ions (PO43-) within 120 minutes, overcoming the limitations of existing costly methods involving palladium catalysts or high-voltage conditioned ozone (O3). Moreover, the process exhibited profitability, with a gross profit of $1.84 per kilogram of phosphorus, presenting a drastic reduction in cost compared to conventional methods using palladium catalysts. This breakthrough not only offers sustainable wastewater purification but also promises a pathway for resource recovery. Additionally, our future prospects involve refining this method into a device capable of purifying industrial wastewater and recovering phosphorus, emphasizing sustainability and reduced power consumption through innovative techniques like using iron and carbon plates forming a battery. This novel technology represents a sustainable solution utilizing abundant resources such as iodine from seawater, iron, and calcium, paving the way for sustainable phosphorus resource recovery.