一等獎

在植物中，對於外在威脅能有效進行防禦一直是個非常重要的課題，而此實驗在探討植物彼此間經由根部傳遞的防禦訊息與生理防禦機制。 利用鹽刺激使其根部分泌出AtCAPE1胜肽進行防禦訊息的傳遞，以證實阿拉伯芥防禦訊息確實可經由根部進行彼此的溝通，且可利用擁有正常基因的植株提升突變株的防禦能力。 結合AtCAPE此種植物體內少有被發現的胜肽進行實驗，並提供了許多延續實驗，為植物防禦系統闢出一條大道。

The main goal of this study was to address a variety of topics concerning the role of the Ih current in HCN channels of SHANK Wild-Type and Knock-Out Thalamus Neurons (as described further below). This research explored the cellular effects of sedation (like Dexmedetomidine) and laser light stimulations on the Ih current of neurons, as well as discovering novel biomarkers for detecting Autism Spectrum Disorder. This study also showed that methods (like utilizing laser therapy with and without various photosensitizers) have the potential in raising depressed Ih currents of SHANK Knock-Out neurons.

隨著大眾對於衛生要求的上升，許多抗菌及消毒成分被廣泛應用於水質處理中，其中次氯酸作為消毒殺菌劑大量使用於泳池及自來水的水質淨化中，然而現行標準方法測定水中有效氯所使用具危害的毒化物且步驟繁雜不利普及民生使用，發展簡便快速且靈敏的偵測方法勢在必行。本研究利用牛血清白蛋白（Bovine serum albumin, BSA）、不同的硫醇分子及金離子合成具螢光特性之硫醇修飾金奈米團簇 （Thiol ligand assists BSA capped gold nanoclusters, BSA/RSH-Au NCs），探討添加不同硫醇分子對所合成之金奈米團簇於不同pH值及常見離子對螢光強度之影響，並利用具有最佳螢光穩定性之2-巰基苯甲酸修飾金奈米團簇（Thiosalicylic acid assists BSA capped gold nanoclusters, BSA/TA-Au NCs），透析後進行次氯酸根檢測，其檢測線性範圍為0.98μM-1000μM，涵蓋法規規定游泳池水及自來水中次氯酸根之容許殘留濃度，最後此方法成功於游泳池水及自來水基質中檢測次氯酸根，分析樣品的回收率介於94.4%-95.6%。此外，在紙上添加金奈米團簇，並加入不同濃度的次氯酸根，觀察其螢光強度的變化，期望此方法未來應用於快篩試紙塗布材料快速檢測水質中次氯酸根濃度。

本研究以添加界面活性劑、二氧化矽及矽油的電流變液作為研究對象，探討矽油的黏度以及添加界面活性劑對電流變液的影響。並希望引入此現象於以高電壓驅動動態摺紙的DLZ(dielectrorphoetic liquid zipping)結構中，增進其性能。 研究發現不同黏度的矽油會對電流變效果造成影響。以低黏度(10cst)的矽油製成的電流變液，黏度變化於6kV時可達原本黏度的2.4倍，而以高黏度(350cst)的矽油製成的電流變液，黏度變化則可達原本黏度的9.7倍。在實驗時也發現，穿透裝有電流變液比色管的光線在施加電壓後減少，黏度越大，減少的幅度越大，並與界面活性劑的存在有關。因此推論: 黏度大的矽油因分子長度較長，藉由界面活性劑作用，更容易和二氧化矽粒子形成整齊結構，因為單一矽油分子可連接更多二氧化矽顆粒。這讓電流變液的黏度改變；同時整齊的結構會使電流變液的散射能力減弱，使接收到的散射光減少。 未來將以顯微鏡直接觀察電流變液的內部結構，並以水及酒精取代界面活性劑，以了解在矽油分子長度改變以及有無添加界面活性劑下電流變液黏度變化的機制，並應用在DLZ結構中，完善原本在單純液體中只有考慮介電常數的理論，增加黏度與液體內部結構的討論。

Alzheimer’s disease (AD) is a neurodegenerative disease in which current diagnostic tools are invasive and lack the ability to diagnose early-onset dementia. Current antibody-based diagnostic tests for neurodegenerative diseases require invasive measures such as a lumbar puncture, and lack specificity to biomarkers that are found in both healthy individuals and patients with AD. In this project, a design for a carbon dot(CD)-bound bispecific antibody is developed for the minimally- invasive diagnosis of AD. The molecular probe can be easily synthesized with a specificity to amyloid- beta (Aβ) oligomers as it distribution and abundance in the brain suggest they are better predictors of disease progression and are present in the early-onset of the dementia. The bispecific antibody conjugated to the CD displays a low affinity to transferrin receptors (TfRs) which allows the probe to cross the blood-brain barrier via receptor mediated transcytosis leading to a minimally invasive diagnosis. A synthesis technique was developed to conjugate the bispecific antibody to the CD. As a proof of concept, this technique was used to couple bovine serum albumin (BSA) to CDs. The structural and optical properties of the CDs were observed. By synthesizing a novel carbon dot conjugated specific antibody that emits light at a specific wavelength in the near-infra red region, the molecular probe displays optical properties suitable for the minimally-invasive diagnosis using fNIR- spectroscopy.

臺灣通過《實驗教育三法》後，許多原住民族實驗教育學校如雨後春筍般成立。鑑於世界美國、加拿大、紐西蘭等國，對於原住民族教育政策提倡「文化回應課程與教學」之理念，本研究以「等差數列與級數」為核心概念，設計「鄒族文化內涵數學文本」與「漢民族文化內涵數學文本」，透過眼動儀技術及數學解題，邀請「都市原住民族學生」、「偏鄉原住民族學生」、「都市漢民族學生」、「偏鄉漢民族學生」等四群學生進行受試。實驗中，每一群學生均須完成「鄒族文化內涵數學文本」與「漢民族文化內涵數學文本」閱讀與解題。受試完畢後，本研究再針對學生所撰寫的數學解題文本與眼動儀相關數據，進行「整體之眼動指標」、「歷程分析之眼動指標」以及「數學問題的解題情形」等三類面向之分析。期望本研究所獲得的結果，也能夠回饋給未來原住民族實驗教育政策的制定或後續原住民族的相關研究上。

本研究旨在探討分散式網路爬蟲瀏覽時間及覆蓋率之最佳化問題原理。藉由相異物排列所形成的循環組關係式進行一系列的探討。在n個相異元素的簡單排列中，不存在任意元素個數為k (k≤n)的子集對應到自己本身所成集合，我們稱此型態的排列方式為k-錯排。換言之，假如n個相異元素進行簡單排列，排列後每個元素都不在原來的位置上，此時這樣的排列稱為一般的錯排列，也就是1-錯排。本研究從分散網路爬蟲搜尋網址中進行相關發想，發現它的本質是遍歷完所有的頂點且沒有重複經過，即所謂「哈密頓路徑(Hamiltonian path)問題」中一筆畫的NP-hard問題，即圖遍歷問題的一種。因此本研究由k-錯排遞迴之性質來探討分散式網路爬蟲最佳化問題。最後透過電腦模擬及組合數學分析推導，本研究將提出改善以k-錯排應用至分散網路爬蟲的最佳化方式。

台灣淡水三角渦蟲 (Girardia tigrina) 主要以水生小動物為食，且會捕食蚊幼蟲，具生物防治潛能 (王與郭，2016)。根據投放前的評估結果，渦蟲對食物無記憶，為機會主義捕食者且會搶食，有利於評估未來投放的數量；野外防治實驗中發現渦蟲可有效抑制模擬水耕地景中蚊幼蟲，且生存水質與蚊幼蟲相似。未來可大範圍投放渦蟲，評估是否能有效抑制蚊幼蟲。本研究另一目標為確認渦蟲消化蚊幼蟲機制。由薄層分析法發現渦蟲黏液需長時間才能水解少量的幾丁質，推測其主要行體外物理消化蚊幼蟲，化學消化則為協助的角色。以石蠟切片發現5 %福馬林加30 %蔗糖之固定液可切出完整的渦蟲咽部組織。未來將持續探討渦蟲消化蚊幼蟲機制，如確認渦蟲捕食蚊幼蟲時咽部肌肉組織變化，以及利用冷凍切片固定渦蟲捕食蚊幼蟲時當下組織，再進行染色觀察。

This underwater remote operating vehicle (ROV) is designed with and without tethered operation. The operator can control the ROV from the real time first-person view in graphical user interface combined with sonar and object detection function when the tether is attached to perform search and rescue. The control tether with fiber optic lighting cable establishes a guided link medium between the possible search victim location and the rescue team. When the tether is detached, rapid deployment by a predefined set of instruction to achieve further operation range. The intelligent technologies of signal processing were used for object recognition, collision detection and sonar scanning data to enhance underwater operation. Autonomous driving is based on software development with limited capability to run in unrestricted open areas. We have achieved the design intent and confirmed the performance data in the laboratory boundary conditions.

My thesis focuses on studying Cretaceous microfossil specimens from the excavation of former brickworks in Štíty, especially foraminifera. In the theoretical part, I have covered the structure of the Bohemian Cretaceous Basin area, especially Bystřice Lithofacial Development. I have also processed previous paleontological researches from the locality. Emphasis was placed on field research and subsequently on laboratory research of the site. I have examined the present state of the location and gathered samples of silt clay containing a wide variety of fossils. I have acquired the microfossils, determined them, and ordered them systematically. The most important part of the thesis is the systematic and palaeoecological processing of the collection of microfossils from the locality. The thesis continues the research of the last year of SOČ, where I have gathered a collection of fossil macrofauna, flora, and ichnofauna. My collection is supplemented mainly by benthic and planktonic foraminifers. I have confirmed that the specimens found are typical representatives of marine fauna belonging to the Upper Cretaceous Coniacian. The paleoecological characteristics of the locality correspond to a nutrient-rich shallow-water environment, occasionally disturbed by storm waves.