全國中小學科展

一等獎

不飽和脂肪酸omega-3對膽管癌的影響

膽管癌是原發性惡性肝腫瘤,其存活率不高且症狀不明顯,診斷出癌症通常已無藥可醫,因此研發抑制的方法極為迫切。許多研究指出不飽和脂肪酸omega-3和抑制發炎反應有密切關聯,發炎反應會使細胞增生,進而有機會導致癌症。本研究使用omega-3,探討其是否能應用於抑制膽管癌。 我們使用兩種對象,一為人類膽管癌細胞株HuCCT1,二為轉基因斑馬魚。我們觀察人類膽管癌細胞,探討omega-3是否抑制其生長情形,並進行即時聚合酶鏈鎖反應測試發炎、細胞週期與細胞轉移基因。在斑馬魚上則研究omega-3能否降低斑馬魚發炎基因的表現量。結果顯示發炎、細胞週期、細胞轉移基因在添加魚油後的膽管癌細胞株中表現量降低。動物實驗中,患有能夠導致膽管癌且有較高比例omega-3的三轉基因魚,其中發炎基因表現量和只具有能夠導致膽管癌的斑馬魚相較有降低。

利用線蟲模式研究飲食對於神經老化的影響及其分子機制

飲食是否影響老化一直是個有趣的議題。秀麗隱桿線蟲有透明體腔、月餘的生命週期,為研究老化的極佳模式生物,和E. coli OP50相比,Comamonas DA1877會加速線蟲生長並縮短壽命。本研究旨在探討DA1877飲食是否造成線蟲早衰及其分子機制。實驗中先確認DA1877加速線蟲生長,並發現食用此飲食的線蟲具有較嚴重的頭部損害且擺尾速度減緩的老化特徵,說明此飲食使線蟲早衰。也採用Pmec-7::mRFP線蟲比較不同飲食下感覺神經老化狀況,更使用aldicarb藥物探討線蟲的癱瘓速率,發現DA1877不僅使線蟲早衰也改變感覺神經型態與運動神經功能。為了探討此現象的分子機制,採用與氧化壓力相關的核心轉錄因子daf-16 基轉種Pdaf-16:DAF-16a/b::GFP比較不同飲食下細胞核螢光顯現量,得知 DA1877使DAF-16入核表現,推測此飲食對線蟲而言為氧化壓力。已知OP50和DA1877在S-adenosyl methionine (SAM)含量上有顯著差異,未來希望以缺乏SAM合成酶之sams-1突變種進行實驗,了解DA1877使線蟲早衰的路徑。

金山神祕海岸浮石事件解密

浮石是一種多孔的火山碎屑岩,氣孔佔總體積很高的比例,因此能浮在水上。本研究針對臺灣新北市金山區神祕海岸所出露沉積層中的浮石進行探討,藉由閱讀文獻、實地考察、採樣、實驗分析等方式,來瞭解浮石的化學組成、礦物種類及可能形成機制與搬運途徑。依照化學成份分析及礦物種類比較結果顯示神祕海岸的浮石與日本、菲律賓的浮石較無關聯,最可能是來自台灣地區並由台灣北部大屯火山系或觀音山噴發所造成。至於其搬運方式,沉積物粒徑大小分析的結果顯示浮石是經由力量強大的海洋搬運作用攜至當地沉積。綜合上述,我們認為金山神祕海岸的浮石最有可能的生成方式為:1867年,由與大屯火山有相同岩漿系統的海底火山噴發出,接著此火山爆發所引發的海嘯將浮石帶至神祕海岸進行沉積。

可重複使用之新型固體酸在藥物合成與生質能的催化應用

生質能源是最佳的能源之一,而固體酸觸媒最能符合綠色化學,因此本研究要開發一種可以水解纖維素得到生質能的固體酸觸媒。將芳香族X與3-氯丙基三甲氧基矽烷反應,產物嫁接至中孔洞SBA-15-p上,再進行磺酸化,得到固體酸TPS-SBA-15-p,將其應用在催化酯化反應及阿斯匹靈的合成,催化效果優於已發表的固體酸觸媒。將TPS-SBA-15-p應用在催化水解纖維素,2 g纖維素與0.5 g觸媒在不鏽鋼高壓反應鍋中加熱至150°C反應7天,轉化率達75.7%,主產物為乙醯丙酸及癸烷,兩者碳數佔全部產物碳數的64%,乙醯丙酸的用途很廣,而癸烷是汽柴油的成分。另外,TPS-SBA-15-p具有兩種催化活性位置,此兩種催化活性官能基具有協同催化作用,因此很容易催化水解二噁烷(dioxane)及苯甲醚,也就具有催化水解纖維素的能力。

Neolema ogloblini- An agent in the biological control of Tradescantia

Tradescantia (Tradescantia fluminensis) is the worst weed in New Zealand. By smothering and shading out seedlings, Tradescantia prevents forest regeneration. Current control methods are ineffective and simultaneously cause harm to native forest. In 2011 Neolema ogloblini, a Brazilian beetle was introduced into New Zealand as a biological control for Tradescantia. To be successful in New Zealand, a country with different environmental factors, the beetles’ ranges of preference (temperature and light intensity) had to be investigated. A gender specific trait also identified, to enable desired sex ratios within founding populations to be selected. [18] This would ensure that the beetles are not released in areas of physiological stress, and can be optimised to have the greatest impact on Tradscantia. To establish how the intensity of light affects the distribution and amount of Tradescantia eaten by N.ogloblini a choice chamber investigation was conducted. Different layers of shade cloth provided a range of light intensities 150-3450Lux (likely to be found under forest canopy where Tradescantia is problematic). Thirty beetles of a range of sizes and approximately same maturity were randomly distributed through the chambers. Each chamber contained a shoot of Tradescantia with 5 leaves. After a 24hour period the number of beetles in each chamber were counted and the amount of surface area of the leaves eaten measured. The effect of temperature on the amount of leaf surface area eaten was investigated by selecting 90 beetles of a range of sizes and withholding food for 24hours. Five beetles were placed in each of three containers containing two leaves. Each trial container was precooled/warmed to the test temperature before the beetles were added. Leaves of a similar size, shape, mass and maturity were used. All leaves were genetically identical and collected from the same location. Sets of three containers were held in the dark at the following temperatures for 24hours: 9°C, 15°C, 20°C, 25°C, 30°C and 35°C. The surface area of leaf eaten at each temperature (mm2) was calculated. Lastly, microscopic dissections were conducted, using 32 beetles ranging in size, to establish if length (measured from the top of the head to the base of the abdomen) could be used as a phenotypic marker to identify beetle gender. While only a very weak positive relationship between increasing light intensity and the number of beetles was found a significantly higher area of leaf was eaten at a light intensity of 3450Lux compared to 150Lux. The amount of leaf area eaten is significantly reduced at temperatures of 15˚C and below, and significantly increased at 35˚C. There is no significant difference in the amount of leaf area eaten when comparing temperatures between 20-30˚C. Females have on average a larger body length (median=4.92mm) than the males (median=4.215mm). Therefore, sites with warmer temperatures in dappled light conditions (3450Lux) should be prioritised for the release of N.ogloblini, as this is the location in New Zealand at which their use as a biological control will be optimised. Beetle length can be confidently used to select desired gender ratios.

Development of a compact, self-stabilizing handheld camera mount

My purpose of the Research was build a small, compact device for the GoPro, which minimizes shocks that are typically caused by running and makes the video stable. The device must fit into a backpack and must be designed for the GoPro. This scenario raised two central questions for me: Which laws of physics are used for the stabilization of the camera? How can those laws of physics constructively be transformed into a working device? I have built four prototypes. To know which prototype was the best, I compared them and gained data into diagrams. The result with the last prototype was very impressive and encouraging, whether your are walking, running or skiing, the videos were no longer shaky. My final prototype consisted of a hollow tube which extended vertically. My conclusions: First the handheld camera mount mustn't be too light, as the inertia of the device is too low. Second it mustn't be too heavy, because you normally have to hold it over a certain time interval. Inertia as well as lever principal were essential to produce a smooth video. The most difficult part to build was the fully gimbaled suspension because it requires as little friction as possible and it must be precise and solid.

Automated Illustration of Text to Improve Semantic Comprehension

Millions of people worldwide suffer from aphasia, a disorder that severely inhibits language comprehension. Medical professionals suggest that individuals with aphasia have a noticeably greater understanding of pictures than of the written or spoken word. Accordingly, we design a text-to-image converter that augments lingual communication, overcoming the highly constrained input strings and predefined output templates of previous work. This project offers four primary contributions. First, we develop an image processing algorithm that finds a simple graphical representation for each noun in the input text by analyzing Hu mo-ments of contours in images from The Noun Project and Bing Images. Next, we construct a da-taset of 700 human-centric action verbs annotated with corresponding body positions. We train support vector machines to match verbs outside the dataset with appropriate body positions. Our system illustrates body positions and emotions with a generic human representation created using iOS’s Core Animation framework. Third, we design an algorithm that maps abstract nouns to concrete ones that can be illustrated easily. To accomplish this, we use spectral clustering to iden-tify 175 abstract noun classes and annotate these classes with representative concrete nouns. Fi-nally, our system parses two datasets of pre-segmented and pre-captioned real-world images (Im-ageClef and Microsoft COCO) to identify graphical patterns that accurately represent semantic relationships between the words in a sentence. Our tests on human subjects establish the system’s effectiveness in communicating text using im-ages. Beyond people with aphasia, our system can assist individuals with Alzheimer’s or Parkin-son’s, travelers located in foreign countries, and children learning how to read.

Development of Biomimetic Skins

The objective of the project is to develop a novel biomimetic membrane and/or a scaffold for the said membrane. The approach of the project is to use animal skin from the domesticated pig or fish as a scaffold material for the adherence and growth of human skin fibroblasts to create a biomimetic membrane that can be used in medical applications as an alternative to today’s gold standards of Xenograft, Allograft and Autograft procedures. The biomimetic skin membrane can be used to treat victims of burns or scarring with a natural material that would be eliminated via natural bodily functions while eliminating the side effects and drawbacks such as scarring, secondary infections and tissue damage resulting from the current gold standard graft procedures on donor sites. Pig and fish skins were treated with ethanol and dehydrated followed by perfusion with Phosphate buffer solution and Cell culture media. Human skin fibroblasts (NF3 cells) were seeded on the animal skin scaffold. The human skin fibroblasts were then observed to determine their morphology and membrane formation properties. It was observed that the human skin fibroblasts were able to adhere to the non-human skin scaffolding and proliferate. More research is needed to determine their viability as a biomimetic membrane.

First photochromic diarylethenes with cyclohexenone ethene "bridge"

Photochromism is determined as reversible transformation between two chemical species, induced by action of light [1]. Herewith, initial form and photoinduced isomer have different properties, first of all, spectral. The phenomenon is attractive for the design of hi-tech materials for different applications, including optical memory elements and molecular switches. Diarylethenes are the most promising class of organic photochromic compounds due to outstanding thermal stability of both isomers and high photostability [2, 3]. Photochromism of diarylethenes explained by reversible electrocyclic reaction of hexatriene system, provoked by UV light, back reaction is induced by visible light. In this work we have proposed a new class of photochromic diarylethenes with cyclohexenone ethene “bridge” 4. The key stage of the synthesis is “one-pot” reaction of ketoesters 1 and chalkones 2 in ethanol in the presence of sodium ethoxide that includes Michael reaction and subsequent intramolecular condensation of the resulting product. The final decarboxylation of semi-product 3 results in target diarylethenes 4. We have prepared a wide range of photochromic diarylethenes with thiophene, oxazole, imidazole and benzene derivatives as aryl moieties. The spectral characteristics of compounds obtained have also been discussed.

Fabrication of Hydrophobic Coatings Using the Sol-Gel Method

The aim of our research is to produce superhydrophobic coatings on both glass and cloth substrates in order to achieve high contact angles and low sliding angles for self-cleaning. In addition, we aim to modify these coatings to be as transparent as possible so as not to interfere with the aesthetics of the objects which will be coated. To achieve this goal, we synthesised a solution using 1H, 1H, 2H, 2H-perfluorooctyltriethoxysilane (a type of FAS), silica nanoparticles (SiO2), tetraethyl orthosilicate (TEOS), (3-glycidyloxypropyl) trimethoxysilane (Glymo) and deionised water. Using the convenient sol-gel method, coatings of 20% and 30% by weight of FAS-SiO2 nanoparticles were prepared on glass and cotton substrates. It was found that coatings containing 30% by weight of FAS-modified SiO2 nanoparticles on glass slide produced coatings with water contact angle as high as 162.8° and sliding angle as low as 4°. It can also be seen that for glass substrates, the hydrophobicity increased with an increase in percentage of FAS-modified SiO2 nanoparticles. Although the highest percentage transmittance was about 30%, texts and pictures beneath the coated glass slides were clearly readable. The cotton substrates also exhibited excellent hydrophobicity, with a water contact angle of 150° and sliding angle of 22°. Furthermore, the substrates showed good retention of colour and durability after simulated washing and 72 hours of ultraviolet (UV) weathering chamber test. These results show that the effects of washing and UV on the important properties of the cloth were insignificant.