全國中小學科展

二等獎

Chlorella vulgaris chlorophyll a fluorescence as a potential indicator for zinc and nickel detection

Heavy metals contaminate many bodies of water, posing a health risk to not only organisms that live and use the water in these areas, but also to the humans that live nearby. Chlorella vulgaris, a microalga, is one organism whose chlorophyll a fluorescence can indicate the presence of these substances, detecting any changes in concentrations using fluorescence microscopy and other fluorescence devices. The study explores the sensitivity of C. vulgaris to the heavy metal zinc where the algae was exposed to five concentrations of zinc: 0 ppm, 5 ppm, 10 ppm, 50 ppm, and 100 ppm. The fluorescence of the samples was observed with a fluorescence microscope on days 0, 4, 7, and 12, where the algal samples were adapted to the dark for 5 minutes, then exposed to light for 90 seconds. The values of the minimal and maximal fluorescence of the samples in the dark were noted. There is a significant difference in the values of the minimal fluorescence, maximal fluorescence, and maximum quantum yield, a value derived from the minimal and maximal fluorescence, at the highest concentration, 100 ppm, from the other treatments for the entirety of the experiment. The significantly low values at 100 ppm and the calculated EC50 of 75.70 ppm indicate that C. vulgaris is indeed a viable indicator for zinc detection at this and higher concentrations of zinc.

合成抗體徵召分子以提升免疫細胞對細菌辨識力之研究

自古以來,致病細菌便是人類免疫系統的強敵,加上近年來抗生素的濫用使細菌在具有抗藥性後更加棘手,相對的人體免疫系統愈顯衰弱。本研究成功合成了一個可以連結細菌和免疫細胞的複合分子,此複合分子是由萬古黴素、連接單元和生物素組成,稱之為複合分子VLB(Vancomycin-Linker-Biotin)。其中萬古黴素用來辨識並結合細菌細胞壁上的肽聚醣,生物素則可以與抗體結合並徵召免疫細胞。 我們以螢光基團及抗體等,實際測試複合分子VLB對細菌的辨識力,確認其能在萬古黴素端與革蘭氏陽性菌連結,並在生物素端與抗體結合。我們藉由複合分子VLB增加細菌及免疫系統之間的親和力,以提升免疫細胞辨識細菌的能力,使其更快更有效地對抗細菌,解決多重抗藥性細菌難以擊敗的問題。利用本研究合成抗體徵召分子的方法,大幅提升免疫細胞對致病細菌的辨識力,將來可望有效地應用在臨床醫學上。

藥物骨架Chromeno [4, 3-b] pyrrolidine的合成方法之探討

Chromene(色烯或苯並吡喃)或chromane(色原烷或2.3-二氫苯并哌喃)及pyrrolidine(吡咯烷)的衍生物是許多藥物和具有生理活性天然物的重要結構單元。含有chromene或chromane的化合物在消炎、抗真菌的研究中表現出良好的活性,而pyrrolidine出現於尼古丁等化合物中。本研究利用亞胺葉立德前驅物與米氏酸衍生物在鹼性催化劑的作用下,篩選出溶劑、催化劑、當量數,找出合成Chromeno [4,3-b] pyrrolidine產率最高的條件。利用此優化條件,改變不同的受質,在苯環上改變不同取代基,探討其反應性,增加其未來應用的多樣性,嘗試利用掌性催化劑,合成出具有光學活性的產物,並建立資料庫。

Co-movement!秋颱共伴效應之深入探討

共伴效應發生的颱風時常帶來嚴重災情,促使我們投入對秋颱共伴效應的分析。利用地面風場、輻合輻散場、衛星雲圖、測站氣壓雨量等統計資料以及颱風路徑等資料分析後,顯示在中央氣象局分類的五號、六號、九號路徑的颱風,發生共伴效應的比例較其他路經之颱風高。在風場與輻合輻散場之比較得知共伴會隨著颱風環流與東北季風相對位置的接近而產生增強的現象,再用地面天氣圖與雨量統計資料確認高壓靠海導致東北季風含水氣量的增加也會加強共伴雲帶降水。利用數十個颱風與六個測站雨量與風向的變化結果相互比較後,得知地形風向效應也會導致當地瞬時及總降雨量增減,而風向與地形走向之夾角越趨近於垂直影響雨量的效果則越顯著,而颱風路徑與共伴地形效應也有極大關聯性。最後利用高空比濕場驗證垂直上空氣舉升效應,確認風向輻合確實會造成氣塊舉升。

Automated Inflation and Pressure Regulation for Recreational and Professional Cyclists

Cycling is a very popular mode of transport as well as a famous sport around the world. Many people enjoy this sport either professionally or recreationally. Cycling in the UK alone has grown up to 200% since lockdown in 2020. (Chandler, 2020) Cyclists make use of a broad selection of products to enhance their performance. Those products range from wireless gear shifting, advanced geometry, smart suspension. This project is aimed to indicate the importance of tire pressure and to introduce a product which will be able to adjust tire pressure while cycling. This product will give cyclist an advantage on different terrains as well as eliminate some common problems amongst cyclists. Flat tires are one of these problems. It occurs commonly amongst cyclists and can happen due to a variety of reasons. Another problem is wrongly inflated tires. This causes unnecessary loss in a cyclist’s power and speeds due to the high rolling resistance between the tires and the surface. This then results in losing time whether racing or commuting. In an article published in 2014 in Velonews.com, Lennard Zinn states: “Whether on tarmac or singletrack, a tire with lower rolling resistance reduces the power required to move forward while also providing a better quality ride. The tire absorbs small bumps by not transferring them into the bicycle and rider, resulting in a smoother ride, faster speeds, and better cornering." (Zinn, 2014) Taking this in consideration it becomes clear that it is important to develop a system which is able to control tire pressure.

探討粒線體如何參與調控細胞內鈣離子訊息傳遞

鈣池調控鈣離子內流(store-operated calcium entry, SOCE)是非興奮性細胞中最主要的鈣離子通道。當內質網缺乏鈣離子時,位在內質網上的STIM1(Stromal interaction molecule 1)便會改變構型與在細胞膜上的ORAI1(Calcium relaease-activated calcium channel protein1)接合,激活SOCE的路徑。近來的研究顯示,粒線體會影響SOCE的活性。已知平均有5%-20%的粒線體藉由連繫蛋白與內質網相連。又已知內職網在缺乏鈣離子時會移動至細胞膜附近,故我們認為粒線體有很大的機率藉由連繫蛋白與內質網一同移動至細胞膜並透過吸收鈣離子的機制來調控SOCE的活性。 在使細胞表現特定螢光蛋白的前提下,我們透過活體細胞攝影來觀察特定對象(粒線體、粒線體內鈣離子)的動態變化。 從實驗結果中我們發現:當SOCE被激活後,粒線體會移動至SOCE發生處且較靠近STIM1。又絕大部分移動至SOCE發生處的粒線體同時也會吸收鈣離子。 過去的研究已證實,當粒線體與內質網之間缺乏鈣離子時,SOCE的活性會降低,且當粒線體內膜的主要鈣離子通道MCU(Mitochondria calcium uniporter)缺乏時,亦會導致相同的結果。又從我們的實驗可知當SOCE被激活時,粒線體會移動至SOCE發生處並吸收鈣離子。綜合上述,我們可以推論以下機制,當細胞內的SOCE被激活時,粒線體會藉由連繫蛋白與內質網一同移動至SOCE發生處,同時以吸收鈣離子的方式來調控SOCE的活性以及細胞內的鈣離子濃度。

Crossing Number of Join Product of Some Graphs

A drawing of a graph G is a representation of G on a plane, with its vertices represented by distinct points, and its edges by arcs connecting the corresponding points. The crossing number of G is the minimum number of intersections between arcs across all possible drawings of G.

A Person Re-identification based Misidentification-proof Person Following Service Robot

Two years ago, I attended a robot contest, in which one of the missions required the robot to follow the pedestrian to complete the task. At that time, I used their demo program to complete the task. Not long after, I found two main issues: 1. The program follows the closest point read by the depth camera, which if I walk close to a wall next to, the robot may likely ‘follow’ the wall. 2. Not to mention if another pedestrian crosses between the robot and the target. Regarding these two issues, I decided to improve it. We’ve designed a procedure of using YOLO Object Detection and Person re-identification to re-identify the target for continuous following.

超立方體最小控制集建構方式的探討

本研究將至多8維的超立方體(hypercube)Qn最小控制集(minimum dominating set)MDS(Qn)建構方式一般化,並藉由同構(isomorphic)的分類討論提出的建構模式之唯一性與否。由文獻得出的各超立方體最小控制集大小γ(Qn)以及已知的控制集形式,並從控制集重複控制的次數R(MDS(Qn )),我們得出Qn的平方圖中最小控制集形成的子圖Qn2 [MDS(Qn )]可能的連通分量(component)數,最後透過Qn層狀圖(layered graph)中各層控制點數的運算,篩選得出可行的建構方式。 研究得出MDS(Q1 )、MDS(Q2)、MDS(Q3)、MDS(Q5)、MDS(Q7)只有一種同構;MDS(Q4)、MDS(Q6)有兩種同構,同時我們發現MDS(Q5)與MDS(Q6)構造上的關聯;Q8的情況較為複雜,我們先是證明了γ(Q8 )=32,並討論MDS(Q8)與MDS(Q7)構造上的關聯,提出了建構MDS(Q8)之方式。

A Person Re-identification based Misidentification-proof Person Following Service Robot

Two years ago, I attended a robot contest, in which one of the missions required the robot to follow the pedestrian to complete the task. At that time, I used their demo program to complete the task. Not long after, I found two main issues: 1. The program follows the closest point read by the depth camera, which if I walk close to a wall next to, the robot may likely ‘follow’ the wall. 2. Not to mention if another pedestrian crosses between the robot and the target. Regarding these two issues, I decided to improve it. We’ve designed a procedure of using YOLO Object Detection and Person re-identification to re-identify the target for continuous following.