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三等獎

探討rVP1在肺癌細胞上Integrin路徑及Wnt/β-catenin路徑的作用機制

經基因重組技術純化的口蹄疫病毒鞘蛋白rVP1被證實在癌細胞內會經由Integrin路徑對癌細胞的轉移及凋亡產生影響。本研究所探討的是rVP1對肺癌幹細胞H1299及肺癌細胞A549中Integrin路徑與Wnt/β-catenin路徑的作用機制,目的除了要了解兩路徑在肺癌細胞中的影響,也希望能找出兩路徑的交互作用機制。 本研究發現肺癌幹細胞H1299中β-catenin會因加入rVP1而表現量下降,與肺癌細胞不同;肺癌細胞A549中Wnt/β-catenin路徑上游的膜上受體Frizzled-8因rVP1作用而表現量下降;而不論在肺癌幹細胞H1299及肺癌細胞A549中Grb2都不會因rVP1作用而表現量有所改變;Wnt/β-catenin路徑下游產物MIG-7則會因加入Wnt3aligand而表現量下降。 由實驗結果推測癌症幹細胞本身與癌細胞特性不同,可能使得Wnt/β-catenin路徑產生的效果不同;Grb2的變化量則需要更多實驗求證。Integrin路徑會影響到Wnt/β-catenin路徑的上游生成,兩者確實有相互影響。

跳線減距問題探討

線段上距離最遠兩點的距離,稱為此圖形的直徑。如果將線段上某兩點用一條跳線連接起來,使得行走路徑能經由跳線快速移動到另一點,以縮短這個線段的直徑。則跳線要加在哪裡,才能使直徑變得最短?會變成多少?將線段想像成一條可彎曲的繩子,假設跳線將兩跳點的距離縮短為0,就好像把繩子上的兩個點「黏」起來,變成一個黏合圖形。藉由黏合圖形,我們研究跳線設置的最佳位置(能讓直徑變的最短)以及最短直徑,得出在線段上加1條、2條和3條跳線時,最短直徑分別為1/2、1/3和1/4,在線段上加k條跳線、黏合圖形中一個和二個節點時,最短直徑皆為1/(k+1)。

Considering Fukushima’s contaminated water treatment system using algae ~ To protect the oceans from radioactive emissions

Nine years ago, the Great East 日本 Earthquake caused the spread of a large amount of radioactive materials. Even now, the amount of contaminated water is increasing at a rate of 180 tons per day, and it is said that the storage tanks for the contaminated water will run out of space in the next two years (Fig. 1). If the contaminated water is discharged into the ocean, it will cause reputational damage to the fishing industry, and the environmental pollution. We are conducting to research to prevent it from happening. In the wake of the nuclear accident, the senior started water quality surveys at Chaya Marsh near the school. During the survey, they found (Chara braunii, Fig. 2), (Nitella axilliformis, Fig. 3), Closterium moniliferum (Fig. 4), and (Nostoc commune, Fig. 5).

Fabrication and Characterization of Biological Electrospinning Nanofiber Scaffold Based on Cellulose Diacetate-Gelatin-Green Tea for Tissue Engineering Applications

Tissue engineering has developed novel therapies such as many types of wound dressings, bio-pads, scaffolds and bandages, in order to reduce the effects of deep and extensive skin wounds. Here, we have produced an electrospun nanofiber scaffold, based on biodegradable materials such as gelatin (as a natural and hydrophilic polymer) and cellulose diacetate (with optimal biodegradability), in order to increase wound healing using nanotechnology. We also used green tea extract for its anti-oxidant and anti-bacterial effect, to improve the biological properties of the scaffold. In the fabrication process, two polymer solutions: 1. Gelatin (with acetic acid solvent) and 2. Cellulose Diacetate (with acetone solvent) mixed with green tea extract, were prepared. Then they were spun using a two-nozzle electrospinner to produce a hybrid nanofiber scaffold. SEM images showed enough finesse and uniformity of the produced scaffold to simulate the extracellular matrix. Further, measuring the contact angle of water droplet and the web surface, indicated optimal hydrophilicity of the nanofiber scaffold, which controls the level of scaffold degradability and cell adhesion. Also, the results of antibacterial tests for two bacterial strains (E. coli and S. aureus) showed the antibacterial characteristics of the extract-containing scaffold. In addition to previous tests, evaluation of fibroblast morphology on the nanofiber scaffolds, indicated appropriate cell adhesion and expansion, that confirms the biocompatibility of this produced scaffold.

自組裝紅色螢光有機分子之合成與其奈米微結構之操控

高科技產業的發展日新月異,創造不同特性的功能材料常扮演推進科技的關鍵角色,若單一材料能以簡易的方式進行奈米尺度下的結構轉換,便可能增加該材料的應用彈性。本研究設計並合成具放紅色螢光性質的雙尿素共軛分子TPDF-Bisurea,並將其引進多孔性陽極氧化鋁(AAO)模板,透過分子兩側雙尿素基團交互辨識,在模板內自組裝形成奈米管。再將奈米管置入充滿THF蒸汽的密閉環境,藉蒸汽微擾分子間作用力,使其由一維管狀轉為零維球狀結構,達到以自組裝行為在分子不同維度間轉換之目的。分析實驗所得的一維與零維奈米材料之基本及光物理性質,期望將兩者應用在光電元件中。其中空結構之特點亦可作為輸送藥物或基因的載體,並藉由螢光性質追蹤其進入目標體後的所在位置。

從「圓」「點」出發—過定點的圓內接多邊形之研究

此研究在探討「給定一圓及多個已知點,求作各邊或其延長線上恰含有前述已知點中之一點的圓內接多邊形」之作圖方法及圖形的性質。 研究當中,以反演的幾何變換結合代數方程式並透過遞迴關係,除了找到該問題之解的個數與條件外,並求得有唯一解之點的軌跡方程式,從中發現其軌跡圖形為一圓錐曲線,且隨著給定點在圓外個數的奇偶性,會有或為橢圓或為雙曲線之不同的圖形變化。同時也發現給定點本身使得滿足條件的圓內接多邊形存在之個數將決定該軌跡圖形與給定圓間之切點個數等的有趣性質。

超高疏水表面水珠碰撞之探討

本研究中探討的是,超高疏水表面上微小水珠結合後並發生向上彈跳的物理現象。此現象近年來被視為能夠應用在眾多領域上,包括增加導熱效率、anti-icing surface等等。本研究從實驗和數值模擬的層面對此現象進行研究,探討水珠在結合中的的表面能的振盪行為以及結合過程中受到的作用力,並提出了一套力學振盪模型來解釋水珠的複雜運動,並且將利用模型討論的結果與模擬結果比較。 在模型驗證之中,我將建立模型中使用的假設給予理論上的誤差,並從兩種觀點進行和實驗數據比較,其中以受力觀點討論水珠碰撞,我所建立的模型誤差在2%以內。最後,對於水珠碰撞在增加導熱效率的應用,我以實驗結果推論並提出了在未來實際應用時應注意的細節。

Dependence of Alloy Composition in Color Change of Brass Foil by Oxide Thin Layer Formation

It is known that copper foil undergoes a color change in heating by oxide thin layer formation. Therefore, we focused on the color change by the oxidation of brass foil. Brass foil (Akaguchi (Cu87%Zn13% alloy) and Aoguchi (Cu85%Zn15% alloy)) also undergoes color change by oxidation, and it shows heating time and temperature dependence. Brass foil need longer heating time to appear color change than copper foil, and we can visually confirm that the brass has corrosion resistant. In addition, color change of brass foil depends on the percentage of copper in the brass, and Aoguchi shows rapidly color change in same heating condition. We show that brass has different physical properties than copper, even with a high percentage of copper in brass, and this was verified through comparison using diffusion length and RGB data in Aoguchi and Akaguchi. We demonstrate these colored brass foils are used as art materials, and our results expanded material using possibility of brass foil.

運用GAN實現字體風格轉換

本研究以實作字體風格轉換的生成對抗網路模型為動機,將Conditional GAN當作模型的基礎,探討pix2pix模型及其他研究的一些方法對模型會產生甚麼影響,以得出能最優化預測成效的深度學習模型。 首先進行的是前處理的步驟,將字體的truetype檔案轉換成模型輸入的jpeg檔,再以生成器(Generator)和判別器(Discriminator)建立Condional GAN的基礎模型,然後探討加入U-Net、Category Embedding等方法,以及訓練資料集大小對模型造成的影響,最後實作整合的pix2pix模型和CycleGAN模型進行比較。 經過實驗後發現,U-Net和Category Embedding都對模型的預測成果有所幫助。另外,對相似的字體而言,pix2pix的效果較好,而對兩種風格差異較大的字體則需用Category Embedding的方式,融入更多字體進行訓練以達到更好的成效。

台灣西南平原表面波現象探討

台灣西南平原的測站紀錄中時常會出現明顯的短周期表面波,此類型表面波常常是因為震波在沖積層共振而產生。本研究利用表面波的低頻、長持續時間的特徵,量化分析2016年美濃地震、2010年甲仙地震與2012年霧台地震中發育的短週期表面波,並與相關圖資套疊,找出與場址特性的關聯。 本研究分析後發現,此三地震皆在西南平原發育出表面波,又以台南七股地區發育得最完整,討論後認為這與此區的地質構造與沖積層厚度有關。量化分析中,時頻圖與頻譜得到的主頻由東到西遞減,與表面波發育情形有近似的分布,持續時間則不明顯,但持續時間的分析因為測站資料不足而有困難,這部分仍待解決。而套疊圖資的結果中,沖積層等深線圖較能符合量化分析的趨勢,土壤液化潛勢圖則否,經確認後發現這與牽涉表面波的地層深度有關。根據本次研究強地動的結果,是能運用在快速掃描沉積地層,較各站鑽探快速且普及。