Utilizing Flavonoids From the Invasive Species Pilea Melastomoides and Daucus Carota as Well as the Protein PTK-2 to Create a Skin Gel Aimed for Burn Wound Healing.
Burns are a major global health concern especially in developing countries like 印尼, where southeast asian women experience the highest burn incidents globally. Burns can cause severe physical and psychological impacts, with treatments that are critical to reduce complications. This study focuses on the development of organic, cost-effective burn gels using flavonoid compounds which are Quercetin and Myrecetin which are taken from pilea melastomoides leaves, a wild 印尼n plant and carrot (Daucus Carota). These skin extracts aim to accelerate wound healing, minimize pain and prevent infection. The gel formation involves extracting active compounds using 96% ethanol as it has been effectively used for extracting a wide range of bioactive compounds to preserve their quality by preventing microbial contamination, and ensures a high yield of active ingredients suitable for topical applications. Then it goes through a process of Phytochemical screening to confirm the presence of flavonoids by using the Shinoda test. The formulation process included dissolving the HPC-m (Hydroxypropyl Cellulose) as a gelling agent, then adding plant extracts (pilea melastomoides leaves and carrot), as well as combining other ingredients such as propylene glycol, sodium benzoate, sodium metabisulfite, and disodium EDTA. The gel was stirred thoroughly to ensure uniformity and left at room temperature for 48 hours to attain the required consistency. The gel that was formatted went under various quality assessments, first being organoleptic testing. This test is used to evaluate its physical characteristics which includes color aroma, and consistency which confirms a stable dark green appearance and a natural strong scent from the plant extracts. The homogeneity test is used to verify the uniformity distribution of active compounds across the gel, to ensure a consistent efficacy. The pH test showed the gel’s acidity level which remained the safe range for skin application. Additionally, the spreading ability test demonstrated the gel’s excellent application properties, with consistent results across trials. Subsequently, the in silico analysis was conducted to predict the behaviour of specific flavonoid compounds used which is the myricetin and quercetin, highlighting their potential anti-inflammatory and antimicrobial activities. Further bacterial contamination tests confirmed the gel’s antimicrobial efficacy, reducing the risk of infection in wounds. This study demonstrates that the gel, formulated with pilea melastomoides leaves and carrot skin extracts, effectively utilizes flavonoids and other phytochemicals to reduce inflammation, promote tissue regeneration and retain moisture, which fosters an optimal condition for wound healing. This organic and sustainable burn treatment utilizes locally sourced ingredients, providing a natural solution that speeds up recovery, reduces pain and prevents infections. The results highlight its significant potential for wider healthcare use, especially in resource-limited environments.
In Silico Carotenoid Compound with Protein in Durian (Durio zibethinus Murr.) Seed Waste and Hedonic Test Innovation in Making Healthy Cereal Organic (HCO) (Nutrient-rich Functional Food Alternative)
Durians’ seeds have potential as a food source due to their content and nutrients. Durians’ seeds contain fiber, minerals, vitamins A, B1, B2, C, carbohydrates, folate, potassium and copper. Nutrients are needed for the body's health and growth and development process. Durians’ seeds have the potential as a nutrient-rich food alternative. Researchers made an innovation in the form of cereal, Healthy Cereal Organic (HCO). Analysis of durians’ seed content through two stages. First, wet lab examination and second, in silico method. The wet lab examination shows the results that durians’ seeds contain 10.17 Kcal of fat energy, 4.09% ash content, 11.25% water content, 72.79% carbohydrates, 1.13% total fat and 10.74% protein and the in silico method shows the content of carotenoid compounds (vitamin A, quercetin, beta-carotene, zeaxanthin) as a drug delivery system which means that this compound is able to be absorbed by the body with the help of albumin as a carrier that maintains stability and increases its activity. Feasibility analysis based on toxicity tests, Durians’ seed compounds show inactive (non-toxic) results. Allergenicity test showed non-allergen durians’ seed content. Hedonic test was conducted on 20 panelists dominant to the HCO1 sample for aroma by 60%, texture 90%, taste 40%. It can be concluded that durians’seeds can be used as a basic ingredient for making nutrient-rich Healthy Cereal Organic (HCO).