免疫治療的新展望：從一個疾病的動物模式，探討樹突狀細胞的培養與分析
胰島素依賴型糖尿病(insulin-dependent diabetes mellitus; IDDM)是一種胰島素無法正常分泌的自體免疫疾病;而NOD老鼠(non-obese diabetic mouse，NOD)的病徵與其非常相似。藉由觀察NOD老鼠發病前後外顯行為及疑導組織切片的差異，我們認為胰島素依賴型糖尿病的致病機轉是因為T細胞工及胰島組織中製造胰島素的β細胞，使胰島素分泌不足而引起糖尿病；而樹突狀細胞(dendritic cell，DC)是調控淋巴細胞反映的重要調節細胞，未來可望利用樹突狀細胞進行胰島素依賴型糖尿病的免疫調控治療。本實驗即是利用IL-4、GM-CSF使NOD老鼠的骨隨幹細胞分化樹突狀細胞，並藉由控制NOD老鼠的年紀與的數突狀細胞培養天數，希望取得較多的數突狀細胞，以利未來免疫治療之用。Insulin-dependent diabetes mellitus (IDDM) is a spontaneously occurring autoimmune disease in which cellular immune components mediate destruction of the insulin-producing βcells of the pancreas. It begins with an asymptomatic stage during theβcells are gradually destroyed. These patients have to depend on injecting insulin to lower their blood glucose, facing the dander of being infected. So we want to research into the cause of IDDM by model animal- NOD mouse (non-obese diabetic mouse). We observe the differences of exterior behavior and sections of pancreas organization between NOD mice and normal ones. It has been shown that the immunophological mechanism of IDDM is T cells destroy βcells of genetically predisposed individuals and result in insufficiency of insulin-producing. Dendritic cells(DC), having great Ag-presenting ability, are related to IDDM. We cultivate bone marrow stem cells of 5-week-old,8-week-old, and 21-week-old NOD mice treated with IL-4,GM-CSF and make them differentiate into dendritic cells. The result shows that using 8-week-old NOD mice to cultivate will get the largest amount of dendritic cells. We also compare the percentage of differentiated DCs for 6 days’ culture with 9 days’,and we find that 9 days’is better. Dendritic cells are the effect Antigen-presenting cells which can be used for immunotherapy of IDDM , though , its complicated mechanism still needs further researching and developing. We hope in the future IDDM patients could get rid of the suffering of injecting insulin in their whole life.
網路監控與家庭自動化系統之研究
The paper presents a new technique for the Internet monition and control system. The user not only can “watch” the current operation of remote home appliances from the client computer, but also directly control the behavior of remote home appliances through the Internet network. In the mean while, concerning the interface between users and appliances, the virtual reality is combined into the system so that users operate the system easily. First, virtual reality, CGI , HEML . I/O control code, 8255 I/O card and CCD are applied in the server computer to control and “watch” home appliances. Next, the client computer is connected to the sever computer through the Internet WWW(World Wide Web) network. Then, the user can control remote home appliances directly through the homepage. Finally, the experiment results are presented and discussed.本研究提出一種高效率且易維護的網路監控技術，從遠端確實“看”到監控系統現場動作，並下達控制指令。同時顧慮到使用者操作介面，結合虛擬實境，讓使用者可更輕鬆操作。首先，伺服端結合VR(虛擬實境)、CGI技術、基本的HTML、I/O控制指令、8255 I/O介面卡及CCD(網路攝影機)，應用於家用電氣，接著用戶端透過WWW全球資訊網連上伺服端首頁，然後依首頁之畫面，來達到遠端監控與家庭自動化之目的。
繪身繪影-正三角形磁磚設計方法與碎形密舖之研究
本研究主要以正三角形作為基本單元，透過窮舉討論得到正三角形邊的作用方式只有五種，再經由排列組合歸納出11 種正三角形密鋪磁磚設計方法。進一步，運用我們的研究結果，配合數學簡報系統製圖，創作新圖樣，也彌補了Escher 在手繪時所造成的誤差，達到完全密鋪的效果。碎形磁磚的部份，我們也依據其背後的數學理論創作幾套結構圖，利用結構解析，碎形密鋪磁磚將變得十分容易，學習者將可輕鬆製作富有創意的新圖樣。 ;This research mainly takes the regular triangle as the basic unit. Through the enumeration, we obtain that there are only five operations for edge of the regular triangle, and then 11 kinds of regular triangle design methods are induced. Even more, utilizing our findings and Mathematical Presentation System (Math PS), we created the new pattern which makes up Escher’s errors and achieves the tiling. As to Fractal Tiling, we create several sets of structure drawings according to its mathematics theory. Using structure analysis, the Fractal Tiling will become extremely simple, and the learner can make the rich creative new pattern easily.
溫度與光週期對淡黃蝶的影響
為了了解淡黃蝶Catopsilia pomona無紋型crocale-like及銀紋型pomona-like中間受到環境因子的差異。先比對兩型的粒線體ＤＮＡ，之後模擬夏季和冬季自然環境進行實驗。得知兩型為同種。另一方面進行溫度和光週期的實驗，顯示淡黃蝶幼蟲和成蟲雌雄個體各部位會受到此兩環境因子的影響。In order to realize if Catopsilia Pomona and Catosilia crocale are the same species, we analyzed and compared the DNA sequences of Mitochondria, and the result revealed they are indeed the same species. Then we observed the developmental process of the butterfly, and inspected the effects of different factors: photoperiod and temperature were shown to affect the phenotype of the butterfly; lower temperature and shorter day resulted in phenotypic shift from crocale-like to pomona-like, and vice versa. Also, the conflicting factors produced intermediated form. (e.g. lower temperature with longer day) Not only changed the phenotypes of adult with photoperiod and temperature, those of larvae also did. However, the mechanism how photoperiod and temperature affect the phenotype of the butterfly is unknown.
台北盆地的熱島效應及其對環境的影響
本研究主要是以台北市中心的氣溫觀測站，以及市區外圍東邊的汐止，南邊的屈尺，以及西邊的山佳等三個氣溫觀測站，從1998 年至2004 年的七年期間的氣溫資料中，來探討台北地區的熱島效應現象。這個研究的結果發現，台北市區與周遭鄰近地區確實會因為熱島效應的影響而產生1~2℃的溫差。這個溫差在白天時不甚明顯，在中午過後，便由台北市中心逐漸向郊區遞減，形成類似同心圓狀的分布。溫差最大值發生在夜晚，使得台北地區晚間宛如一座夜晚增溫的城市，同時夏季的熱島效應又較冬季顯著，氣溫又逐年遞升，造成夏季台北市區的高溫屢創新高，將是未來都市發展的危機。This study is focused on the urban heat island effect of Taipei Basin, northern Taiwan. The hourly temperatures of Taipei meteorological station and three rural sites eastern Hsi-Chih, southern Cyu-Chih and western San-Chia, were compared from 1998 through 2004 to illustrate the temperature differences between city center and surrounding country areas. The results show that a difference of 1~2℃ exists between city center and surrounding country areas, indicating the extent of urban heat island effect in the Taipei Basin. The daily temperature contours show a clear high-temperature bull’s eye at the city center during the midnight, implying the high latent heat trapped by the dense and high rise buildings. This phenomenon is not sharp in the high noon due to the reduction of temperature difference between city center and surrounding country areas. The heat island effect is most prominent in the summer than that of the winter. Along with the increasing temperature-difference trend through years, the summer times often experience record-breaking heat waves and pose great risks for the city development in the future.
直接乙醇燃料電池之觸媒層研究
直接乙醇燃料電池以酒精與氧氣透過氧化還原反應產生電能，但化學反應緩慢，需利用觸媒以增加其速率。本實驗目的在於盡可能找出一個表現最佳的觸媒。本實驗利用活性碳粉作為觸媒（鉑、錫）的載體，以酸性（HNO3）與鹼性（NaOH）環境分別處理碳粉，再以含浸法與多元醇含浸法將觸媒還原。我們得到以HNO3 處理的碳粉無法保有原碳粉的型態，較利用NaOH 處理為差。在觸媒製備方面，多元醇含浸法還原效果比含浸法可得到較小的觸媒尺寸，在本實驗中，溶液中鉑與加入的碳粉重量比為3:7 時，可得到最大的反應面積。此外，當鉑與錫原子數比為4:1 時，可得到最大的乙醇氧化電流。Direct ethanol fuel cell is a kind of power source which generates electrical power by a redox reaction involving ethanol fuel and oxygen. However, this reaction takes place slowly; therefore, catalyst is needed to improve its activity. The goal of this project is to get an optimize catalysts ratio to obtain the best catalyst activity. Activated carbon is used as the support of catalyst (platinum and tin) particles in this project, which is pre-treated in acid (HNO3) and alkaline (NaOH) solutions respectively. Then, the precursor is reduced by impregnation and EG-impregnation. We learned that activated carbon pre-treated with NaOH activates better than which pre-treated with HNO3 because the latter bear less resemblance than the former. As for the catalyst, the results of EG-impregnation show smaller size of catalyst particles than those of impregnation. In this project, when the ratio of the weight of platinum and activated carbon added into the solution is 3:7, we can get the largest surface area. In addition, when the ratio of the amount of platinum and tin atoms is 4:1, we can get the largest current of ethanol oxidation.
魚洗水跳現象的探討
In the previous year study, we assumed that the fish basin as a column-shape model in order to exam and explain how the water spouting. This year, we established a reality-like situation of the fish basin to construct our theory base. At this point, round-waves and up-down waves are found as the beginning point of the water spouting. The sound from the basin is the frequency of the vibrations. Meanwhile, this process enables us to understand the causes of four, six, and eight waves. In addition, we tried to change the basin's positions, the capacities of water, the densities of liquids, and the sizes of the containers to demonstrate the theory. Furthermore, we used similar containers, such as high-heel cup and big magnetic bowl to experiment. The results are also corresponded with the theory. These results can also be used to explain the Chinese aeolian bell, clock, shaking bell, etc. That is a major innovation for us. Finally, we obtain better understanding of the abstractive theory though the simulation of the computerized photographs. 魚洗，也稱龍洗，傳說是中國唐宋時代皇帝洗臉的臉盆，內底四條魚紋，麟尾畢具。洗內盛水後，用手摩擦其兩個雙耳，立即發出響亮的嗡鳴聲，並出現美麗了漣漪，水珠四濺，摩擦越快，聲音越響，波浪翻騰，水珠越烈。對此在上一年度的研究中，是將洗盆視為圓柱狀，然後由圓柱狀所建立的模型去解釋水跳的原因，初步瞭解了水跳的原因。今年我們以洗盆真實的情況建立理論模型，在殼體產生共振的圓形駐波及上下駐波，此為水跳產生點，此時殼體發出的聲音就是共振的頻率。也瞭解了有四個、六個、八個、十個波腹的原因。並以改變殼體不同位置、水量、液體密度及洗盆大小等變因應證模型，結果與理論相符合。繼而以高腳杯、大磁碗等類似魚洗盆構造的容器，加以實驗應證，也能符合此理論。甚可推到中國古時候的編鐘、搖鈴、風鈴等器材。對於我們總認為碰撞就產生聲音的觀念實是一大突破。最後經由電腦動畫的模擬，讓此抽象的理論更容易瞭解。
倒車攝影機影像之變形校正
與傳統的鏡頭比較起來，廣角鏡頭可取得較大範圍的影像，所以廣泛地運用在很多地方，例如倒車攝影機、內視鏡、防盜警眼等，但是其影像會有很大的變形失真，容易造成人類眼睛的錯覺與誤判。本篇研究以倒車攝影機為例，使用了多項式函數來校正其影像的變形與失真，從程式實作的校正結果看來，我們的方法之效果還不錯，相信同樣的模式可用來校正其他使用廣角鏡頭的儀器設備之變形影像。 Compared with the traditional lens, wide-angle lens can make images of larger range, so they are widely used in a lot of places, such as move backward cine-cameras, laparoscopes, and burglarproof eyes. The drawback is that the images distort a lot and may cause the illusion to human eyes and lead to erroneous judgments. This reserch takes the backward cine-camera as example and utilizes multinomial function to correct the deformation and distortion of the image. The results show that the method we use are quite effective and the model can be applied to other equipment with wide-angle lens.
彩色蠶繭之研究
近幾年來，蠶繭彩色化已引起廣泛的注意，日本與中國大陸紛紛投入此研究領域。我們用食用色素、酸性染料和活體染色劑中性紅等色素水溶液，以沾附於桑葉餵食、浸泡蠶體、注射入蠶體血腔等方法，使白色繭品系的家蠶生產出多種顏色的彩色蠶繭，其中以附於桑葉餵食最有效率，我們就此法找出投與色素的有效期間，可以比日本、中國的方法更節省色素。同法處理黃色品系的家蠶則產生黃色與所加色素的混合色蠶繭。由於這樣得到的彩色蠶繭放久了都會褪色，我們試用奈米色素餵食家蠶所得彩色蠶繭，與一般食用色素所製成的彩色蠶繭比較，發現對各種光照、清潔劑清洗等處理，用奈米色素所得蠶繭明顯較用一般食用色素所得蠶繭不易褪色。這樣用奈米色素生產的彩色蠶繭，因為解決了褪色的問題而更具有潛在的產業價值。To make silkworm cocoons with different colors has received a great attention recently. Japan and China have invested great resources in this field of the study. In order to let white cocoon silkworms produce cocoons of different colors, we used the aqueous solutions of food dyes, acid dyes and neutral red, and fed the worms with mulberry leaves immersed with such aqueous solutions, or directly soaked or injected them with the solutions. We found that using mulberry leaves immersed in the dye solutions was the best approach. We improved this approach by finding a critical, effective time of applying dyes. It saved the dyes and labor than those of Japan and China. We also found that yellow cocoon silkworms produced yellow and mixing colored cocoons by the mulberry leaf feeding method with the same dyes. Because all colors of the cocoons mentioned above faded easily, we furthermore tested nano-dye and found that colors of the cocoons had better resistant to fading away in washing with detergents under various types and intensities of light illumination. This result suggested that nano-dye has a potential in solving the fading problem of the colored cocoons.