全國中小學科展

四等獎

AUTONOMIC HEATING GLOVES

In today's world, medicine is very advanced, thanks to which many diseases that were previously considered incurable, are now treated almost all over the world. But, unfortunately, some diseases are still incurable and can only facilitate their course. One such disease is Raynaud's disease or Raynaud's syndrome. Statistics show that worldwide the percentage of patients with this disease is 3-4%. Raynaud's disease is a paroxysmal spasm of the arteries of the fingers of the hand, rarely the feet when cooling the extremities. As mentioned earlier, this disease is incurable. That is why the creation of a device that can help people overcome many inconveniences due to the inability to stay in the cold without gloves or the problem of discomfort in heated gloves is relevant. And one of the solutions to this problem is to create special heated gloves. This work is also relevant, because even existing treatments, such as medication and conservative, do not completely solve the problem of reducing the sensitivity of the hands when cooled or even the slightest moisture on the palms. Also, these methods are very expensive, so our device will be cheaper and more affordable than existing ones. When using our gloves together with the two already mentioned methods, the treatment will be more effective. Unfortunately, medical and conservative treatments will lead to complications over time, so we not only maintain sensitivity in the hands, but also prevent further amputation of the upper extremities and the emergence of human health problems associated with the effects of drugs on the whole body. Nowadays, people work hard to be able to live well, but it is difficult for people with Raynaud's phenomenon to do so, as the sensitivity of the upper extremities decreases during the exacerbation of the disease. It is important for us to maintain the sensitivity of the hands by normalizing the thermal balance of the hands, which leads to the elimination of spasms of the atria of the hand. The aim of the work is to create a simple and effective means to normalize and maintain the thermal balance of the upper extremities, in order to reduce the loss of sensitivity of the hands in patients, as well as reduce the likelihood of spasms of the arteries of the fingers. The subject of the study is the course of Raynaud's disease and the current treatments for it. The aim of the study is the creation of special gloves that can stop spasms of arteries and maintain blood flow in them by balancing the heat balance in the hand, and depriving patients of the disease during their wearing During the work the following tasks were set: - to theoretically investigate the peculiarities of Raynaud's disease; - to analyze the existing clothes on the market with heating; - to develop and improve its own design of heated gloves, which will be affordable and easy to use. - calculate the cost of gloves taking into account all factors

簡易方法測量電遷移率

氯化鈉水溶液滴上染料,外加互相垂直的電場和磁場後,離子受到和電流方向垂直的勞侖茲力而移動,因黏滯力作用而形成漩渦偶極子;由漩渦偶極子的位置隨時間變化,可得到漩渦偶極子的運動動量通量,進而算出鈉離子和氯離子的電遷移率和(μ++μ-)。 用氫氧化鈉水溶液實驗時,因正離子的電遷移率小於負離子,漩渦偶極子一面移動一面偏轉,由移動的位置隨時間的變化可求得鈉離子和氫氧離子的電遷移率和(μ++μ-); 由偏轉量可求得兩種離子的電遷移率差(μ--μ+)。用氯化氫水溶液實驗時,漩渦偶極子偏轉方向和前者相反,由偏轉量可求得兩種離子的電遷移率差(μ+-μ-)。 霍爾效應實驗可求得離子的電遷移率差,用氯化鈉、氫氧化鈉、氯化氫、氮氣奈米氣泡、和氧氣奈米氣泡水溶液作實驗,和漩渦偶極子法對照比較。

MafF對LRH-1調控代謝機制之探討

已知 liver receptor homolog-1(LRH-1)掌控著多種生理功能,在肝臟代謝中扮演著相當重要的角色,我們利用酵母菌雙雜交技術(Yeast two-hybrid)找出數種可能跟LRH-1產生交互作用的蛋白質,並從其中選擇v-maf avian musculoaponeurotic fibrosarcoma oncogene homolog F (MafF)作為主要研究對象,探討其與LRH-1之間的作用對代謝造成的影響。本研究中,我們利用免疫沉澱法證實MafF可與LRH-1形成複合體,而MafF並不影響LRH-1蛋白的表現量。隨後以啟動子活性檢測MafF-LRH-1複合體在細胞內之功能,發現MafF能促進由LRH-1所調控的small heterodimer partner (SHP)、glucokinase IV (Gck)及side chain cleavage enzyme (SCC)等代謝相關啟動子之活性。此外我們以GST-pull down發現MafF與LRH-1結合區域位於LRH-1的DNA 鍵結位(DBD)。LRH-1則是僅與具有完整basic region (BR)以及leucine zipper (LZ) 兩個domain的MafF產生交互作用,單獨的BR以及LZ無法與LRH-1結合。另外,在肝臟細胞株HepG2中過度表現Myc-MafF,Western blot結果發現GCK有減少的趨勢,SHP則是有增加的現象,而LRH-1亦呈現增多的趨勢。Real-time PCR結果,比對Myc以及Myc-MafF,發現MafF存在時會使LRH-1以及GCK mRNA含量較控制組Myc有減少的趨勢,對SHP mRNA 則是沒有顯著影響。此外,我們在肝臟細胞株HepG2中加入胰島素,發現二者表現量皆有明顯的增加,說明胰島素刺激之下,MafF以及LRH-1蛋白質增加能夠增強肝臟能量代謝。

以二階段電壓/電流步進陽極處理法探討TiO2奈米管製備及應用

本實驗以二階段電壓/電流步進陽極處理法生長二氧化鈦奈米管,並找出最佳生長參數並能有效降解有機物。在實驗過程中,我們發現以氟化銨及有機醇類在形成二氧化鈦奈米管,有著較長管徑的效果。本驗以20 x 20 mm2大小的鈦片,透過鈦片的前處理,不同程序的氧化步驟及不同的醇類來探討二氧化鈦奈米管進行降解亞甲藍的效果,以及二氧化鈦奈米管其生長情形。結果發現以化學處理及陽極氧化處理過的鈦片,在0.6w%的氟化銨2ml的水及98%的乙二醇當作溶劑,以每分鐘調整1V直至50V在恆壓一小時的電壓步進法進行陽極處理,最後以450度進行退火,最後可得其二氧化鈦奈米管有最佳的降解效果。

熱休克蛋白Hspb7基因表現調控之探討

小分子熱休克蛋白 B7 (HSPB7,Heat shock protein B7),又稱心血管熱休克蛋白 (cvHsp)。HSPB7表現於心肌細胞當中也表現在骨骼肌當中。我們可以從再生的肌肉細胞中看到熱休克蛋白B7大量的表現,可見其對於損傷的肌肉細胞至關重要。此外,Mef2a (Myocyte-specific enhancer factor 2A) 被發現對於Hspb7基因表現有所影響且、屬於AP-1 (Activated Protein 1) 家族的c-Jun (Jun proto-oncogene) 和Fra-2 (Fos-related antigen 2) 被發現影響了肌肉發育和參與了骨骼肌衛星細胞群的調控。 本實驗在HEK-293T細胞當中將克隆出的Mef2a、c-Jun、Fra-2與Hspb7上游不同長度且帶有螢火蟲螢光素基因之啟動子進行轉染實驗,並藉由螢光素酶檢測法觀察彼此調控關係。本研究發現,c-Jun、Fra-2對於Hspb7基因表現有抑制的成效而Mef2a有增加基因表現的成效。

Schaubcylindrichnus生痕化石化學分析與生成環境探討

根據在北海岸地質考察所觀察到生痕化石種類分布,大部分生痕化石生成於濱海帶,可以推測大寮層及南港層在尚未抬升時,沉積於濱海帶的砂層沉積。另外,石底層雖然同為海相,但屬於海退時期,生物活動區域相對於海進時期較為深,因此生痕化石數量較少。將地質考察採集到Schaubcylindrichnus進行初步的外形觀察,推測其為S.coronus型態以及S.formosus型態,並將Schaubcylindrichnus與Ophiomorpha製成薄片進行比較,發現Schaubcylindrichnus與Ophiomorpha都是由石英與有機物組成,但Schaubcylindrichnus的顆粒比Ophiomorpha小且較為密集,而在Schaubcylindrichnus與Ophiomorpha中,管壁中與管壁內外的有機物含量有明顯差異,Schaubcylindrichnus管壁中的有機物較管壁內外少,而Ophiomorpha管壁中比較多,且可以發現大塊有機物包裹數塊石英的壁襯結構。

太陽軌跡在車輛儲能之創新研究

本研究探討太陽軌跡在車輛儲能之創新應用,使得太陽電池以簡單的方法得到最大的吸收能量。首先,利用Stellarium免費天文軟體,完成一個太陽電池最佳方位角與仰角的計算公式,基於光學與鏡面反射原理之瞭解,提出全新的鏡面最佳轉角定理,使鏡面反射之轉角得以迅速精確計算。本文引入GPS衛星定位功能,將這個太陽電池能量追蹤方法,由靜態的的地面應用,推展到動態的車輛應用。最後,由本實驗結果顯示,利用本文提出的創新研究,與單純平放太陽電池之儲能作法比較,若將反射鏡或者太陽電池隨時對準最佳方位角與仰角,可以獲得多出20 %〜52 % 的免費能量!

以直向、橫向、斜向磁磚鋪滿mxn矩形的研究

我們的主題是研究使用直向、橫向與斜向磁磚不交疊地鋪滿m × n的長方形的方法總數。磁磚總共有如圖A中間所列出的四種(其中著上藍色和綠色兩者統稱為「斜向的」磁磚),並且斜向磁磚不能夠有圖所顯示的交疊的情形。在1961 年的論文中給出了只使用直向、橫向磁磚鋪滿2m × 2n 的長方形的方法數的一個漂亮的公式,而2001 年的論文以一個較簡單的方法證明了這個結果。(1)(2)(3) 而我們先從固定 m 尋找遞迴式的方法出發,接著用m 的結果推出m + 1,最後期望能找出與論文一樣的一個漂亮的一般通式。

利用水稻Near Isogenic Line篩選調控磷酸吸收相關之基因

因應人口增加,提升作物產量同時維持農業永續經營成為全球發展的重要課題,其中如何減少肥料使用以降低對環境衝擊為一重要關鍵;磷肥為作物生長必要元素,過度使用不僅加速礦產資源耗竭且造成環境汙染,若能提高作物磷吸收效率將可望減輕上述問題。水稻為世界三大主食之一,本實驗目的在於了解水稻吸收磷的調控機制,作為未來孕育高吸收效率水稻的基礎。選用稉稻(台農67號)及秈稻(台中在來1號)來檢測長期缺磷後再回復磷供給的狀況下這二個品種的生長狀況及地上部磷累積情形,結果顯示台中在來1號的根明顯較短,而台農67號第二葉磷酸累積較高,接著利用兩品種雜交所得之190個Near Isogenic Line進一步篩選磷酸吸收效率表現良好之品系,於養分充足下培養,或及在長期低磷培養後回復供給4天,比較各品系株高及幼葉和老葉磷酸累積量,未來將利用已知的基因多型性圖譜剖析造成差異之基因座,以了解水稻調控磷酸吸收的分子機制。

Synthesis of Biodegradable Plastic From Food Waste

Based on NEA Waste Statistics and Overall Recycling Rate for 2017, 809,800 tonnes of food waste and 815,200 tonnes of plastic waste was generated. Both food waste and plastic waste account for more than 10% of the total waste generated in Singapore in 2017 respectively. However only 16% of the food waste and 6% of plastic waste was recycled, the rest of it was disposed at the incineration plants and then the landfill. Such action will eventually lead to 2 major environmental issues that Singapore will face in near future: 1)Semakau landfill is our only landfill left and it is expected to run out of space in near future 2)The burning of food waste results in the release of methane (CH4), a greenhouse gas that has over 25 times the impact in trapping excess heat in the atmosphere as compared to Carbon Dioxide (CO2). This will increase carbon footprint and contribute to greenhouse effect and global warming in due course. According to the Sustainable Singapore Blueprint 2015, Singapore is working towards becoming a Zero Waste Nation by reducing our consumption, reusing and recycling all materials. A national recycling rate target of 70% has been set for 2030 with an aim to increase domestic recycling rate from 20% in 2013 to 30% by 2030 and non-domestic recycling rate from 77% in 2013 to 81% by 2030. As part of our total commitment towards waste management and sustainability effort, the purpose of doing this research project is to investigate whether food waste can be recycled and made into biodegradable plastics. First of all, chitosan will be derived from shrimp shells and be dissolved in acetic acid and lactic acid produced by probiotic fermentation of fruit and/ or vegetable waste for synthesis of biodegradable plastics.