防鏽小尖兵(分子自組薄膜的探索)
自組薄膜(SAMs, self-assembled monolayers)乃是具有特定官能基的化合物在不需外力作用 下自動吸附到基質表面上而自行排列成有自序規則之結構。SAMs技術因製備容易且穩定，在 應用上深具潛力。目前此方法中，以硫醇接在Au上的研究最為廣泛。本作品希望藉著SAMs 方法將硫醇分子吸附於鐵片或其他金屬表面上，以達成防鏽與抗酸目的。我們分別透過接觸 角量測研究正十二硫醇在鐵片上形成分子薄膜的可能性；利用酸與金屬產生氣體的速率研究 分子薄膜抗酸蝕的情況；測試分子薄膜的耐熱性；根據鐵片生銹時pH值變化、重量變化與[Fe2+] 含量差異來探究分子薄膜能否防鏽；最後找出適合形成分子薄膜的濃度與溫度效應。由實驗 結果我們發現SAMs薄膜確實能吸附於鐵(及鎳、銅、鋅等金屬)的表面上並增加抗酸與防鏽蝕 能力，薄膜對熱的穩定性極佳，35℃時結合效果較佳，而濃度的提高有助於SAMs的效用。 Self-assembled monolayers(SAMs) are elements which have specific functions.SAMs,ordered molecular aggregates can automatically adhere to the surface of substrate without any force.The applcation of SAMs’technique has high potential not only because ther are easy to make but also because they are stable.Exposing molecules such as alkyl thiols to an Au(0) surface is now in widespread use. In this work,we apply the thiols chemisorb onto the Fe or other metal surface to make it rust-resistant and acid-resistant.We study the following issues to find the appropriate conditions of forming monolayers in varying concentration and temperture: I. The possibility of forming n-dodecanthiol molecular monolayers on Fe surface by measuring the contact angles. II. The ability of antiacid corrodibility by comparing the rates of producing gas from acid and metal. III. The heat-resistant of molecular monolayers. IV. Whether it’s antirust by detecting the changes of pH,weight,and the concentrations of Fe2+ during the iron rust. According to the results,we conclude SAMs do adhere to the surface of Fe(and other matal like Ni,Cu,Zn), which increases the ability of antiacid and antirust.Besides they are stable to heat, have good combining effect at 35℃, and it is beneficial to the effect of SAMs through raising their concentration.
愛的教育，鐵的紀律-果蠅的學習與記憶
The main purpose of this study is to explore whether fruit flies (Drosophila melanogasters which background is 2U) can distinguish and memorize different colors and smells by learning. Futhermore, to find out the relation between age and learning ability, and analyze the influence of repeated training on how long the memory would last. According to our experiment, in smell training, the fruit flies are able to distinguish and memorize different smells, and young fruit flies have better memory than old flies. In color training, the fruit flies can distinguish and memorize different colors, but the effect was less than that of smell training. In intensive learning, proper times of learning increased the memory ability, but excessive learning exhibited an opposite effect. In the labyrinth experiment, we select and nurture the fruit flies having the best color memory in order to further understand the gene correlated with the memory learning. In addition, in one-time training, fruit flies are able to remember the smells and colors in four minutes, and young ones have the better ability. In six-time training, fruit flies have the ability to remember the smells and colors in twelve minutes. In the end, we hope you can know more about the learning and memory ability of fruit flies through our report. 本實驗主要探討果蠅是否可經由學習分辨、記憶顏色與氣味，並探討年齡與學習能力的相關性、分析多次訓練對記憶時間的影響。由實驗結果得知，氣味方面，果蠅有辨識記憶氣味的能力，以年輕果蠅的效果較佳；顏色訓練方面，果蠅亦可分辨記憶顏色；密集學習方面，適當學習次數內，有助於增加果蠅的記憶能力，但過度的學習刺激會降低學習效果。並且，我們選顏色訓練中記憶較佳的一組進行迷宮實驗，經三次的岔路選擇，選出對顏色記憶最佳的果蠅，希望能探討訓練過的果蠅子代能否有較優良的記憶。至於記憶時間方面，一次氣味學習，果蠅在四分鐘內皆有記憶，且年輕果蠅記憶效果較強，達六分鐘；另外，多次氣味學習，果蠅於第四次訓練效果最佳，且記憶能力可達十二分鐘。希望藉本實驗報告，以期更瞭解果蠅學習與記憶的資料。
應用生物特徵及視覺密碼學防治車輛失竊和酒醉駕車
車輛遭竊以及酒醉駕車對社會安全造成的危害普遍存在於世界各先進國家。以目前看來，此問題並非嚴刑峻罰就能解決的。若本研究計畫將來普及應用於大眾，必定可以同時減少車輛遭竊之機會和預先防範酒醉者開車駕駛。 生物特徵泛指指紋、虹膜、臉型、聲紋甚至DNA 等等，它的特性即每個人均具有此獨一無二的性徵，加以應用就如同無法被仿造或偷竊的身分證明。若車主能利用自己的生物特徵為車輛上鎖，即可防止車輛遭竊。 視覺密碼學是屬於資訊隱藏技術的一種，藉由兩張以上的影像疊合後呈現其中隱藏之機密資料。雖然視覺密碼學原先純粹是為了加密資料，但我們將之加以修改，設計出複雜且無意義的偽裝影像，使其不易被酒醉後神智不清的人疊合成功而讀出其中的加密資料。 結合生物特徵及視覺密碼學，即可設計出一套可安裝於車上的系統，不僅防止車輛失竊又能防止酒醉駕車事故的發生。 ;The damage which is caused to social security by car stealing and drunk driving universally exist in others developed countries. As things are, these problems can not be solved by severe law. If our project can be applied in the masses extensively, it must be able to prevent both car stealing and drunk driving. Roughly speaking, biological characteristics mean fingerprint, iris, face, pronunciation and DNA. Their uniqueness is a special feature that everyone has. If we can apply it more, the characteristics are identification that can not be imitated or stolen. If the car owner can use his or her biological characteristics to lock the car, the car stealing could be prevented. Visual cryptography belongs to a kind of information hiding. By stacking two or more images, user can recognize the secret information hiding in the stacking. Although visual cryptography is merely created for encryption at first, we modify it and design a complex as well as meaningless stego-image. That makes the unconscious driver who is drunk fail to both stack the images and recognize the secret information hiding inside. We combine biological characteristics with visual cryptography and design a suit of system that is able to install in the car. This system can prevent not only car stealing but also drunk driving.
以Geobacillus thermoleovorans T4 菌株轉化農業廢棄纖維素為葡萄糖以發展生質酒精
我們得知從高雄糖廠及堆肥中篩出的嗜高溫好氧菌Geobacillus thermoleovorans T4，是本種細菌首先被報導具有纖維素分解能力的菌株，但目前僅止於學術研究階段，尚未實際應用於廢棄纖維素的分解上。本研究以廢棄農作物纖維素取代學術研究所用的羧甲基纖維素(Carboxymethyl Cellulose，CMC) ，將T4菌株置於稻稈與米糠培養基內，進行分解效能比較，再利用酵母菌的發酵作用進一步將葡萄糖轉化為酒精。本研究發現，在60℃的環境中，活化的T4 菌株可在CMC、稻稈及米糠培養基中生長繁殖且發揮其分解纖維素為葡萄糖的能力，尤其在米糠培養基中的分解效果最快也最好。此外，將生產的葡萄糖加入酵母菌之後，初步發現也能成功地進行發酵作用產生酒精，生質酒精的產出指日可待！;The Research on Using Geobacillus thermoleovorans T4 to Turn the Deserted Cellulose in Agriculture into glucose in Order to Produce Bio-Renewable Energy It has been found that Geobacillus thermoleovorans T4, a thermophilic aerobic bacterial strain isolated from a sugar refinery wastewater （55-60℃）in Kaohsing, Taiwan, can secrete thermostable endocellulase and hydrolyze carboxymethylcellulose （CMC）in some academic research, but it is stillunknown whether T4 hydrolyzes deserted cellulose in Agriculture. The aim of this study is to investigate the best conditions of T4 cellulase activity after mixing with deserted cellulose (such as rice bran and rice straw) by measuring the glucose concentration and bacteria number, and to produce the ethanol by activated yeast. T4 was added rice bran and rice straw medium, and cultured in 60℃ for 10 hours. The number of T4 and the concentration of glucose were measured every two hours. The best conditions were examined by comparing the hydrolyzation efficiency of T4 in different cellulose medium. We observed that T4 grew efficiently in different cellulose medium and hydrolyzed cellulose into glucose, especially in rice bran medium. The yeast also converted glucose into ethanol. Our research may shed light to the development of bio-renewable energy!
安培植物假說
在「植物的秘密生命」這本書中提到植物在電場或磁場中會生長的更好，我最近正好學到了電磁場如何產生，以及一些產生電磁場的方法，所以本研究即利用螺形管線圈來產生均勻的電磁場，並用運用安培右手定則來控制磁場產生的方向，來探討電磁場在不同條件下對實驗植物－綠豆生長的影響。本實驗使用銅質線圈纏繞鐵碗的方式，且通入不同大小電流來改變磁場的強度，以探討對實驗植物－綠豆生長的影響。本研究所得到的結果顯示：(1)綠豆在通入電流50mA 時所產生的電磁場可促使綠豆達到最好的生長效果；(2)栽培綠豆環境中所架設的電磁場較高，可使綠豆表現出較為明顯的生長速率；(3)促進綠豆的生長並不需要二十四小時都通入電流；(4)綠豆在N 極朝上的電磁場中生長的速率較S 極朝上的磁場中要快；(5)在較低溫度環境中，電磁場促進綠豆生長的效應較為顯著。;It has been mentioned that the plants will grow faster in the electronic or magnetic field in the book of “the secret life of plants”, I learned about the knowledge and methods of how to produce the electromagnetic field just now, I use the screw wire to produce electromagnetic field and control the direction by the Amplifier’s rule to investigate the growing speed of green beans under electromagnetic field in those different temperature. In this experiment, I use the wire to screwed around the ironed bowl, and make different electric current to generate different strength of electromagnetic field, the results indicate that (1) The faster growing speed will only exist in some strength of electromagnetic field, (2) The higher ironed bowls that full of screwed wire will result in the faster growing speed, (3) It will not need electromagnetic field all days, (4) The green beans will grow fast in those north magnetic pole than those in south magnetic pole, (5)The effect of electromagnetic field to increase growing speed will be significant in higher room temperature.
螺旋狀剝皮對樹木影響之初步研究
本研究的目的在於探討螺旋狀剝皮對植物生存以及芭樂果實的影響。實驗的設計是將選擇的植株或其枝幹分成四組，分別施予環狀剝皮、螺旋狀剝皮一圈、螺旋狀剝皮三圈及不剝皮等處理。 研究結果顯示，螺旋狀剝皮不會導致植株死亡，且於處理部位下方會長出新的枝葉。芭樂果實經100 天的生長之後，不剝皮處理之枝幹長出的芭樂重量都在 300g 以下，而螺旋狀剝皮一圈之枝幹長出的芭樂有重達300-400g(5%)及 400-500g(5%)，最重的達 490g；螺旋狀剝皮三圈之枝幹長出的芭樂也有重達 300-400g(占 7.7%)。此外，與不剝皮處理者比較之，螺旋狀剝皮也有助於高甜度芭樂比例的提升。 本研究成果若能成功應用在其他果樹上，有助於提高果農產收的經濟價值。 The purpose of our study is to examine spiral bark-stripping’s effects on trees, and observe what will happen with this treatment, especially in the survival of trees and fruit of Guava. The experimental design is as below. First, we divided tree samples or branches into 4 groups randomly, and then treated each group differently with girdling, spiral bark-stripping a circle, spiral bark-stripping 3 circles, or non-stripping on the trunks or branches. As a result, spiral bark-stripping did not cause death of trees. Instead, new green leaves grew below treated area. After 100 days of growth, the fruit of Guava treated with non-stripping weighed below 300 grams, while some fruit weighing above 300-400 grams(5%) and 400-500 grams(5%) grew on those trees treated with spiral bark-stripping a circle, with the heaviest of 490 grams. Besides, there are some fruit weighing 300-400 grams(7.7%) growing on those trees treated with piral bark-stripping 3 circles. The result shows that spiral bark-stripping, compared to non-stripping, promoted the proportion of high-sugar fruit. This study provides a possible way to increase the economic value of fruit harvest if applied to other kinds of fruit trees.
STATIN類降血脂藥物對血管平滑肌細胞之作用
動脈硬化是個致病率和致死率相當高的慢性發炎疾病，為台灣十大死因之一。在病理過程中血中濃度過高的膽固醇為動脈硬化的一大危險因子，其會誘發一連串的發炎反應驅使血管壁內皮細胞功能喪失，血壓上升，平滑肌細胞增生等。Statin 是臨床上十分有效的降血脂藥物，雖然其作用機制已知在於抑制膽固醇合成酵素 HMG-CoA reductase 而有降血脂功效，但近年來探討 statin 在抗發炎方面的作用也漸受重視。nitric oxide synthase (NOS) 代謝產物如 nitric oxide (NO)，cyclooxygenase (COX) 代謝產物如 prostaglandin (PGE?、PGI?)，及 heme oxygenase-1 (HO-1) 代謝產物如 carbon monoxide (CO)，均有文獻指出可以改善血流，而可能在動脈硬化上扮演保護角色。相反的，matrix metalloproteinase (MMP) 的表現會誘使更多的免疫細胞浸潤到血管壁，並增加動脈硬化斑破裂，引起栓塞和中風的發生。在此實驗中，我們利用培養的大鼠主動脈血管平滑肌細胞作為研究材料，發現了 statin (lovastatin 、pravastatin、atorvastatin 、fluvastatin) 具有一些和降血脂無關的直接保護血管壁能力。包括會增加 interleukin-1β (IL-1β) 所誘導 iNOS 蛋白的表現及NO 的產生; statin 本身會增加 COX-2 和 HO-1 蛋白的表現及 PGE? 和PGI? 的產生，及抑制 MMP-2 和 MMP-9 蛋白活性的表現。此外分析調控 iNOS 基因轉錄最為關鍵的基因轉錄因子 NF-κB，發現適量的 statin 會增加 IL-1β 活化NF-κB 的作用。值得一提的是雖然適量 NO 有維持血管恆定的功能，過量時則會造成血壓過低休克的現象，這就是細菌感染後因內毒素 lipopolysaccharide (LPS) 作用引發敗血性休克的主要原因之一。為更進一步釐清 statin 是否會影響受細菌感染病人的生命危險，我們也探討 statin 對LPS 作用的影響。結果發現 statin 反而會抑制 LPS 誘導大量 iNOS 蛋白的表現，NO 的產生及 NF-κB 的活化。這些新的實驗結果提供更多證據支持 statin 可以藉由維持血管舒張，減緩血管壁的發炎反應，穩定動脈硬化斑的作用，以有效控制動脈硬化各個病程的進展。這發現能讓我們更透徹明白 statin 的作用，且對將來研發 statin 在心血管疾病方面新的臨床治療用途是有所助益的。 ;The 3-hydroxy-3-methylglutaryl (HMG)-CoA reductase inhibitors, statins, are potent inhibitors of cholesterol synthesis and have wide therapeutic use in cardiovascular diseases. Recent evidence, however, suggests that the beneficial effects of statins may extend beyond their action on serum cholesterol levels. In this study, we investigated the effects of lovastatin, pravastatin, atorvastatin, and fluvastatin on cultured rat vascular smooth muscle cells. We found statins can inhibit LPS-induced iNOS expression and NO production, while they can potentiate IL-1β-elicited responses. Moreover, statins themselves can stimulate COX-2 expression, PGE?, PGI? formation, and HO-1 induction. In contrast, statins can inhibit basal and IL-1β-induced enzyme activities of MMP-9 and MMP-2. In studying the activity of NF-κB, which plays an important role for iNOS gene induction, we found that statin can increase IL-1β-induced NF-κB activity, while inhibit that induced by LPS. All these results suggest that stimulation of iNOS expression in the presence of IL-1β, togeth1er with the increased COX-2 and HO-1 expression might contribute to the beneficial effects of statins in atherosclerotic process in terms of vasodilation and inhibition of smooth muscle cell proliferation. The inhibition of MMP activity might enhance plaque stability and reduce the development of atherosclerosis. All these results strengthen the pleiotropic actions of statins in anti-inflammation and anti-atherosclerosis.