台北市內湖金面山區兩棲爬行動物資源調查之研究
本研究之目的主要為調查臺北市內湖金面山區附近兩棲、爬行動物之物種概況，建立內湖地區的兩棲爬行動物基本生態資料庫，期望藉此研究可推行高中科學教育研究的基礎，並可充實自然生態保育與愛護鄉土心靈教育的實際教材。 調查研究工作是由2003年4月開始，至2004年5月為止，十四個月期間，總計85次的夜間野外觀察，已確認記錄兩棲爬行動物的種類共計有37種(分屬2綱3目13科24屬)，蛙類：4科13種，蜥蜴類：4科9種，蛇類：4科13種，龜鱉類1科2種。 各物種總出現隻次方面，蛙類以拉都希氏蛙的1547隻次為最多，最少的是長腳赤蛙及斯文豪氏赤蛙，僅6隻次。蜥蜴類以黃口攀蜥的1995隻次最多，最少的為麗紋石龍子，僅4隻次；蛇類及龜鱉類以龜殼花最多，共計85隻次，而以梭德氏游蛇、大頭蛇、白梅花蛇、赤背松柏根、南蛇、盲蛇、食蛇龜及材棺龜等均只有1隻次為最少。 出現頻度方面，十四個月份中，蛙類的拉都希氏蛙及古氏赤蛙皆有出現，在85次的調查期間，則是以拉都希氏蛙的97.65％為最高，最少的為長腳赤蛙，只有3.53％；蜥蜴類的黃口攀蜥於十四個月份皆有出現，而在85次的調查期間，則有75次、97.65％最高出現頻度，麗紋石龍子於14個月份及85次的調查，出現頻度均是最低，各是21.43％及3.53％；蛇類及龜鱉類物種中，以龜殼花14個月及61次的出現記錄為最高，出現頻度分別是100％及71.76％；而食蛇龜及材棺龜均只有出現一個月及一次，故其出現頻度為7.14％及1.18％。 分佈廣度而言，於二十二個調查樣區中，蛙類以拉都希氏蛙的95.45％為最大，最小的為長腳赤蛙及斯文豪氏赤蛙的18.18％；蜥蜴類以黃口攀蜥的100％為最大，，最小的是無疣蜥虎的9.09％；蛇類則以龜殼花的86.36％為最大；龜鱉類物種皆僅4.55％的分佈廣度。 各樣區出現物種數方面，蛙類以Ａ區的11種為最多，蜥蜴類以二期校區的7種為最多，蛇類是以ＡＢ區的9種為多；各樣區中，則是以ＡＢ區所發現的兩棲爬行動物物種數最多，合計共有22種物種。 由研究結果得知，調查樣區內的兩棲爬行動物物種歧異度大，其中蛙類的優勢物種為：拉都希氏蛙，蜥蜴的優勢物種為：黃口攀蜥，而龜殼花則為蛇類的優勢物種。而環境溫度的變化，與調查樣區內兩棲爬行動物出現活動的總物種數有顯著之相關性。 ;The goal of this research paper is mainly to investigate Taipei city, Neihu District, nearby Jin-Mian mountain’s amphibious and reptiles for the establishment of the lake area amphibious and reptile basic ecology information bank. It is hoped that this research will provide a foundation to further promulgate the high school science education and research, and may enrich the natural ecology protection and education materials for the loving care of local environment. The investigation started from April, 2003 to May, 2004, or for 14 months period and amounted to 85 times of field observations at night. It was confirmed that there were 37 species of amphibious and reptile ( 2 classes 3 aria 13 families 24genus),amphibious species: 4 families 13 species, lizard: 4 families 9 species, snake: 4 families 13 species, turtle: 1 family 2 species. In regard to the number of times of appearances, amphibious species of Rana latouchii had the most appearances with 1,547 times, and the least appearances was the Rana longicrus and the Rana swinhoana with only 6 times; for the lizards, Japalura polygonata xanthostoma had the most appearances with 1,995 times, and the least appearances was Eumeces elegans with only 4 times; for the snake and turtle, Trimeresurus mucrosquamatus had the most appearances with 85 times; for the Amphiesma sauteri sauteri, Boiga kraepelini, Lycodon ruhstrati ruhstrati, Oligodon formosanus, Ptyas mucosus, Ramphotyphlops braminus , Cistoclemmys flavomarginatn and Mauremys mutica , each had appeared only 1 time. On the appearance frequency, within the 14 months, amphibious species Rana latouchii and Rana kuhlii, Rana latouchii appreared 97.65%, the highest within the 85 investigations period, and the least was the Rana longicrus with only 3.53%; lizard Japalura polygonata xanthostoma had appeared within these 14 months, but in 85 investigations period, their frequency was 75 times, or 97.65% at the highest frequency. Eumeces elegans there upon within the 14 months and 85 investigations, had the lowest frequency each at 21.43% and 3.53%; for the snake and the turtle species, Trimeresurus mucrosquamatus within the 14 months and 61 appearances had the highest frequency record at 100% and 71.76%; but Cistoclemmys flavomarginata and Mauremys mutica only appeared once in a month and, therefore its frequency was at 7.14% and 1.18%. For the distribution breadth, in 22 investigation sample areas, amphibious species take Rana latouchii of the amphibious kind had the biggest record at 95.45%, and the smallest was Rana longicrus and Rana swinhoana at 18.18%; for the lizards, Japalura polygonata xanthostoma was 100% as the biggest, and the smallest was Hemidactylus bowringii at 9.09%; for the snake, the Trimeresurus mucrosquamatus was at 86.36% as the biggest; the turtle species had only 4.55% distribution breadths. For the number of appearance in each area, amphibious species in area A had the most with 11 species, lizard of second period school area had the most with 7 species, the snake of area AB had the most with 9 species. In all other areas, the amphibious reptile species number in area AB had the most with 22 kind of species. From the results of the research, the biodiversity of amphibious and reptile species within the investigation areas is very high, Rana latouchi is the codominant of Frog; Japalura polygonata xanthostoma is the codominant of Lizard, and Trimeresurus mucrosquamatus is the codominant of Snake.
酒杯發出之音符
When you draw a wet finger around the edge of a half filled wine glass, a sweet musical sound comes forth. The pitch of this sound is directly correlated to the amount of liquid in the glass- the higher the height of the liquid is, the lower the frequency is. It means that the shorter the air column in the glass is, the lower the frequency is. This phenomenon differs from the variance in pitch in a wind instrument. In a wind instrument such as a flute, the shorter the air column in its chamber is, the higher the resulting pitches are. In order to study the wine glass phenomenon, we used a piezoelectric crystal loudspeaker connected to an oscilloscope. We recorded the resulting data by using a digital video recorder to capture the images of the waveform of sound, and than analyzed the waveform by using the computer. Our conclusions are as follows: 1. The frequency of sound thus produced was the same whether we draw our finger around the rim, or we strike the glass rim. The higher the height of the liquid is, the lower the frequency is. But the frequencies vary when we strike the glass and when we blow on the edge. 2. When we used a glass without liquid in it, the frequency emitted when we drew our finger around the edge, this frequency varied inversely as the cube root of their weights. 3. In a glass with liquid, the emitted frequency did not have any correlation to the weight of the contents. By taking two identically filled glasses and placing in each a solid object of the same size but different weight, we were able to see that there was no change in the frequency emitted between the two glasses as long as the height of the liquid remained constant. 4. According to “The Flying Circus of Physics”, if we tap the side of a glass of beer, because of the air bubbles in the beer, the frequency emitted will be lower than that from a glass of pure water. This is according to the book, because the speed of sound is lower in air than in water, therefore the speed of sound in an air-water mixture would be lower than in pure water. The resonant frequencies of the mixture will also be lower. However, in our experiment, we discovered that\r when the glass contained air bubbles, the frequency emitted higher. Our explanation is that the sound emitted since the rim of the glass oscillated transversely, the frequency depends only on the retard of the rim and that the frequency is independent of the speed of sound. The intention of this research is to clarify the many misconceptions of this interesting phenomenon.以溼的手指在玻璃酒杯邊緣摩擦，會有悅耳的聲音，而且頻率會隨著內裝液體減少（空氣柱變長）而變高，這種變化與管樂器隨空氣柱的變長而音調變低不同，為了研究它的原因，我們利用壓電晶片喇叭連接到示波器上，並且利用數位錄影機錄下示波器上的訊號，再以電腦分析出瞬間的頻率，結果發現：一、摩玻璃杯與敲玻璃杯，杯所發出之頻率相同，都是所裝液體愈多發出之頻率愈低。但敲玻璃管與吹玻璃管所發出之頻率不同。二、不裝液體之高腳杯，摩擦時所發出之頻率與重量之立方根成反比。（與鐘相同）\r 三、裝液體之高腳杯發出之頻率，不再與總重量有關，而是與液體之高度有關，保持液體高度不變，即使在杯子中央加入不同重量之固體，杯子振動頻率還是不變。若改裝不同密度之液體，則密度愈大頻率愈低。四、在“The Flying Circus of Physics”書中提到輕敲裝有啤酒之杯時，會因杯中含有氣泡而聽到較低之音調，書中解釋是”空氣中之音速低於水中之音速，混有空氣之水中音速變低，其共振頻率也會降低。”但我們的實驗結果是有氣泡時頻率反而高。我們的解釋是杯子所發出之聲音是由於杯面之振動也就是杯壁的橫向振盪，振盪頻率與液體對杯壁之阻尼有關，但與液中聲速無關，密度愈大之液體阻尼愈大。有氣泡時接觸杯壁之液體變少，阻尼較少所以頻率高。希望本研究能使大多數人對這有趣之現象不再有誤解。
吸〝氣〞大法-QCM 對有機氣體之吸附與偵測
中文摘要：\r 化學實驗中，常常會使用到各種具有揮發性的有機溶劑，如醇類、酸類、醛類及酮類等。\r 當吸入過量的揮發性有機溶劑時，將會對人體造成嚴重的傷害，故使用時格外需要留意。有\r 鑑於此，我們開始著手研究如何偵測氣相中的有機氣體分子。\r 本研究中，我們利用聚苯胺薄膜吸附氣體分子的特性並結合對質量變化極靈敏的石英晶\r 體微天平，自行設計了一套簡單、藥品用量少且不需昂貴儀器的實驗系統。藉由此系統，我\r 們有效的針對各種揮發性有機溶劑進行偵測，並進一步地探討不同氣體分子對於聚苯胺薄膜\r 的吸附現象。\r \r 英文摘要：\r Students usually use volatile organic solvent in laboratorys such as alcohols, carboxylic acids,\r aldehydes, ketones, etc. It will injure our body when we breathe in too much volatile organic\r solvent. For this purpose, we begin to study how to detect volatile organic compound in air.\r In this work, we combine polyaniline membrane which can adsorb gas molecules and quartz\r crystal microbalance which is highly sensitive to mass changes to design a simple and inexpensive\r system which needs only little chemicals. With this system, we can effectively detect different\r volatile organic compounds. Furthermore, we can also study the adsorption of polyaniline\r membrance for different volatile organic compounds.
大氣層厚度光學測量法之研究及創新
這個專題研究的目的是要發展出一套簡單可靠的方法和廉價自製的器材，在地面上即能有效估測大氣層的厚度。我們小組研究光學中雷氏(Rayleigh)散射的原理，針對空氣分子對光線散射作用和特定方向之偏極效應，利用一已知散射長度之路徑，測量其偏極光的強度，同時比對由大氣層散射而來，在同一偏極面上的散射光強度，即能估算大氣層的厚度，方法簡單新穎，自製器材經實際測量和改進，有發展和推廣的價值。\r The main idea of the experiment is to set a system in order to effectively estimate the thickness of the atmosphere. On the theory of “Rayleigh Scattering” (small air molecules sizing about 10-4μm), we developed an equipment that has two tubes. The tubes lead the scattered lights from two paths. One is called “air light” scattered in the ground air, and the other “sky light” is scattered in the sky and reflected by a beam splitter. The two paths are on the same plane; the scattered lights are perpendicular to the direction of sunlight and 100% polarized. We could adjust and measure the distance “d” of the air light path. We simultaneously observe and compare the intensity of the lights from the two paths with the electronic instrument made by ourselves. By using the known distance “d” and the reflection “x” of the beam splitter, we can calculate the thickness of the atmosphere. The experiment is simple, novel and easy to do in an extensive field at school. Researchers don’t have to use a bloom, radar or satellite to discover the atmosphere, but you could use a simple equipment to observe the features of it.
阿拉伯芥AtYAK1 基因5'UTR 中的開放讀序框(uORFs)對基因表現調控之探討
在模式植物──阿拉伯芥（Arabidopsis thaliana）中，AtYAK1（Arabidopsis thaliana Yak1-related protein kinase）是目前發現唯一屬於DYRK（Dual specificity Yak1-Related protein Kinase）的蛋白激?。雖然之前研究已證明，不同物種之DYRKs 和細胞的生長與發育過程有關。然而，其在植物中的生理功能卻尚未被明確地研究報導過。在先前的研究中，為瞭解AtYAK1 在阿拉伯芥內作用之位置，前人選取AtYAK1 基因ATG 上游約2.5 kb 的序列（Upstream Element, 2.5KUSE）建構至一含有GUS（β-glucuronidase）報告基因的質體中，並轉形至阿拉伯芥，進行GUS 組織染色分析。但在初步結果中，並沒有在轉殖株觀察到明顯的GUS 表現。進一步分析，我們發現在2.5KUSE 序列末端約0.5 kb 的5’非轉譯區（5’untranslated region, 5’UTR）中，有四組開放讀序框（Upstream Open Reading Frame, uORF）。有趣的是，許多研究也顯示，uORFs 會影響轉譯過程中的再起始（re-initiation）作用而調控該基因的表現。另一方面，前人亦透過構築好的2KUSE 轉殖株（即不含有5’UTR）進行上述GUS 實驗。結果發現，此2KUSE 轉殖株的GUS 表現非常顯著。本實驗即要瞭解AtYAK1 的uORFs 是否也會影響其蛋白質的合成。首先，我們以點突變的方式將四組uORFs 中之ATG 換成TTG，目的為構築不含有uORFs 之5’UTR（mutated uORFs, ΔuORFs）。在進行原生質體短暫表現分析法（protoplast transient assay）及GUS 組織染色分析後，將結果與含有uORFs 的結果作比較：當缺乏uORFs 後，其3’端報告基因的表現量確實比原來顯著。綜合以上，我們認為此uORFs 對於AtYAK1 蛋白質之表現佔有相當重要的影響地位。最後，我們對5’非轉譯區是否存在開放讀序框進行阿拉伯芥全基因組分析，相關結果亦於本研究報告中分析討論。AtYAK1（Arabidopsis thaliana Yak1-related protein kinase）is the first DYRK（Dual specificity Yak1-Related protein Kinase ） family member identified in the model plant ─ Arabidopsis thaliana and exists as one copy gene in Arabidopsis. Previous studies showed that many eukaryotic DYRKs are involved in regulating the growth and development of cells. However, the study of AtYAK1 in Arabidopsis is lacking to date. In order to understand where AtYAK1 expresses and functions in plants, a 2.5 kb fragment which is located upstream from the major ATG of AtYAK1（termed Upstream Element, 2.5KUSE）was previously constructed to drive the expression of a reporter gene, GUS（β-glucuronidase）, in transgenic Arabidopsis. Much to our surprise, no GUS expression signal could be detected in such transgenic plants. When further analyses were performed, we found that there are four upstream open reading frames （uORFs） in the 5’untranslated region ( 5’UTR ) within the 2.5KUSE. Many studies indicating that the uORFs can regulate the translation of downstream ORF encoding the major gene product through the procedure of translation re-initiation. This action represents a mode of translational regulation for gene expression. Indeed, GUS activity could be readily detected in transgenic plants expression 2KUSE::GUS, a construct lacking the 5’UTR of AtYAK1. In this study, I have tried to elucidate whether the uORFs of AtYAK1 can regulate the translation of the downstream major ORF. First, in order to construct a 5’UTR fragment of which uORFs have been mutated（ΔuORFs）, we apply site-directed mutagenesis to substitute ATG with TTG for the four uORFs and examine the expression of GUS driven by this mutated 2.5KUSE. After analyzing the results in both Arabidopsis protoplast transient assay and transgenic Arabidopsis, stronger expression of reporter genes in both systems were observed when the four uORFs were mutated. We have also confirmed that, in transient expression system, the increase of reporter gene activity was not due to the excess accumulation of the corresponding mRNAs. Rather, it is the four uORFs which play an important role in negatively regulating the translation of AtYAK1, possibly via inhibiting the translation re-initiation of major ORF. A genome-wide examination of uORFs in all Arabidopsis genes was also performed to assess the possible contribution of uORF in regulating gene expression.
「金」枝「玉」葉—金奈米與葉綠素的交互作用
本實驗在探討，當金奈米粒子和植物中的葉綠素產生鍵結作用力時，能量轉移的結果是否能幫助葉綠素激發電子。經由兩者混合後光譜的變化,發現兩者之間發生能量轉移。為探討此轉移現象和濃度的關係，我們將大小不同的金奈米和不同毫升數的葉綠素作用，並將其結果和金奈米與葉綠素的吸收強度和作比較，使用正規化的計算方法算出比值，由此看出兩者之間能量轉移的效率。當金顆粒約大於30nm時，正規化的數值隨的葉綠素濃度的增加而變大；而當金奈米顆粒約小於30nm時，則隨著葉綠素的增加而變小。Much attention is currently focused on chromophoric molecules because they can not only mimic natural antenna systems but also exhibit unique optical and physical properties. Chlorophyll , produced by extracting from green leaves, has electrostatic interactions with Au nanoparticles through carboxyl groups. Herein, we report the charge transfer between chlorophyll and Au nanoparticles using UV-vis electronic absorption spectroscopy. The efficiency of charge transfer from chlorophyll to Au nanoparticles was estimated by the normalization of Q-ban absorption intensity. From the observation of absorption intensity versus concentration of chlorophyll curves, we find that the efficiency of charge transfer is increased while the size of Au-particle is larger than 30nm, but decreased while the size smaller than 30nm.