澱粉?抑制劑之研究
植物合成澱粉?抑制劑可以對抗動物的取食，國外實驗證明數種澱粉?抑制劑對害蟲防 治具有顯著效果，其中以腰豆(Phaseolus vulgaris)研究最多。我們利用5% T.C.A.進行粗萃，從台灣常見豆類中篩選出四季豆(與腰豆同種不同品系)與菜豆，對麗蠅的澱粉?具有明顯的抑制效果，對豬胰臟與黃豆澱粉?的抑制效果則小或無，此種抑制特異性深具害蟲防治的潛力。經由溫度與pH 的試驗發現粗萃中的澱粉?抑制劑成分應為蛋白質。我們以四季豆作為繼續研究的對象，將粗萃進一步純化，經由陰離子交換與膠體過濾層析，分離出單一蛋白質，經蛋 白質定序比對確認其可能為國外發表的腰豆澱粉?抑制劑—αAI-1。經由測試發現此抑制劑在 85℃時仍能抑制果蠅澱粉?，為一相當穩定的蛋白質；且抑制劑的作用受pH 值影響很大，在偏酸性環境下的效果最好，與昆蟲分泌澱粉?的部位亦為酸性環境有相當密切的關聯；且其 抑制作用具特異性，可明顯抑制果蠅、入侵紅火蟻、白蟻、蟑螂及麵包蟲等昆蟲的澱粉?活性，對人類唾液、豬胰臟、四季豆本身及黃豆澱粉?的抑制效果很小或無，值得繼續深入研究。 Plant amylase inhibitors can fight against predation from plant-eating animals. It has been reported that several amylase inhibitors have an obvious effect on pest control; among them that from Phaseolus vulgaris got the most surveyed. 5% T.C.A was employed to make crude extracts. We have screened the amylase inhibitor activities from crude extract among beans common in Taiwan. The inhibitors from both string beans (the different strain of Phaseolus vulgaris) and cowpea notably inhibited the amylases in Chrysomia megacephala, but little or no inhibition in porcine pancreas and soy bean. This specific inhibition behavior suggested strong potential in pest control. Its activity can be affected by temperature and pH suggested that amylase inhibitors in crude extracts should be proteins. String beans were chosen to be further purified from the crude extracts. A single protein was isolated after ion exchange and gel filtration chromatography. Through protein sequencing, the partial amino acid sequences were highly homologous to that ofαAI-1 from Phaseolus vulgaris, indicating it might beαAI-1. The purified protein still can inhibit the amylase from Drosophila melanogaster at 85℃, suggesting it is thermal-stable. Its activity was affected by pH and reached the peak in weak acidic environment, which might be related to the fact that amylases are secreted in acidic environment of insect’s midgut. It obviously inhibited the amylases from D. melanogaster,Solenopsis invicta, Odontotermes formosanus, Periplaneta Americana Linnaeus, and Alphitobius sp., while not to human saliva, porcine pancreas, soy bean and string beans itself. The unique pattern of inhibition activities of the purified amylase inhibitor was worthy of further anlysis.
這裡真安靜-隔音材質的探討
本實驗針對隔音材質的特性，如各孔板之孔徑、開孔率，並以夾層做不同孔板配置進行研究。本主題共進行三階段的實驗。第一階段探討不同材質的影響，因材質加工問題，實驗誤差過大；第二階段改以PP 塑膠板探討，因儀器誤差，數據難以解析；第三階段仍以PP 塑膠板探討，並修正實驗步驟，以降低實驗誤差。本報告乃以第三階段實驗結果做探討。為了提高實驗準確度，將整個實驗分成若干組，在實驗前後以空白測試，以減低實驗誤差。利用Excel 軟體，進行各組實驗數據噪音傳送損失之修正與分析。本試驗以125Hz、250Hz、500Hz、1000Hz、2000Hz 及4000Hz 六段週波頻率做測試。由分析結果顯示，孔徑與開孔率、同心圓的排列方式，對噪音傳送損失有顯著的影響。;This experiment is to focus on the properties of sound barriers, such as hole diameter, open areas, and the layout of holing boards. We have performed experiments in three steps. In the first step, we discussed the differences between different kinds of material but found many errors caused from faults in the process of making boards. So in the second step, we chose the ploy-propene board but still found errors from instruments. And in the final step, we used the ploy-propene board again and modified the experiment procedure to lower the error from instruments. The report is based on the final step data. With a view to raise the precision of the experiment, we divided the experiment into several parts with the blank test. We modified and transferred the original data to the noisy transfer loss (TL) in the same intensity level, and analyzed the data with Microsoft Excel. In experiment, we detected the noise-pollution level (dB) in the frequency of 125Hz, 250Hz, 500Hz, 1000Hz, 2000Hz and 4000Hz. According to the result, the hole diameter, open area and the layout of concentric circle have a significant influence on TL.
乳酸桿菌及啤酒酵母之相互作用及其代謝產物對Clostridium difficile 生長之影響
市面上可以看到多種品牌紛紛推出了以“啤酒酵母”作為號召的乳製品，標榜健康取向\r 的“啤酒酵母優酪乳”也是其中的一件，我們蒐集了關於啤酒酵母以及乳酸桿菌的文獻，打\r 算研究之間的關係，乳酸菌屬於益生菌，主要的用途是製作優酪乳，可以協助維持體內正常\r 的腸道菌相，降低血清膽固醇以及預防骨質疏鬆症(Rolfe, 2000)；啤酒酵母最主要的用途為\r 啤酒的製作，具有解決失眠、消除疲勞、改善痢疾的功效。外國的克芙爾(kefir)也是屬於含\r 有乳酸桿菌及酵母菌菌相的食品，而且也對健康有益。因此我們提出了一個問題﹕啤酒酵母\r 與乳酸桿菌的代謝產物是否有著互利的功效呢？在拋出第一個問號後我們繼續尋找資料以\r 及著手我們的實驗。在文獻中(Gaon et al.,2003；Corthier et al.,1992,1986)也曾經提及啤酒酵\r 母與乳酸桿菌對於Clostridium difficile 所引起的偽膜性腸炎的關係，所以使我們又想問兩個\r 問題：究竟Ampicillin 對於乳酸桿菌、酵母菌、C. difficile 的影響有多大？而乳酸桿菌及酵\r 母菌對於C. difficile 的影響又是什麼？
溫差電池的熱力學研究與應用
溫差電池中若僅進行的反應，則其電池電壓與溫差成正比，且純粹是利用化學反應將熱能轉換成電能，我們稱之為「典型溫差電池」，由熱力學公式可推導出典型溫差電池的電動勢(ΔS = S(s)—S(aq)，S為絕對熵， n為得失電子數，1F = 96487 C )，且得到下列三項推論來說明溫差電池的特殊現象。 (1) 同一溫差電池，其電動勢與溫差成正比 (ε∝ ΔT)。(2) 不同的溫差電池，當溫差一定時，電壓ε 與ΔS 成正比，與得失電子數n 成反比。典型溫差電池中，電解液濃度越小，金屬離子濃度也愈小，會使得ΔS = (S(s)—S(aq))的絕對值變大，因此溫差電池的電壓也就愈大。(3) ΔS 值的正負決定電壓ε 的正負。Cu(NO3)2 及ZnSO4 溫差電池的ΔS 為正值，所以高溫杯為正極；AgNO3 溫差電池的ΔS 為負值，所以高溫杯為負極。因水溶液中陰、陽離子不能單獨存在，所以單一離子水溶液的絕對熵無法求得，但科學家把氫離子水溶液的標準絕對熵定為零，藉以求出其它離子的絕對熵，然而我們測得在一定溫差時典型溫差電池的電動勢ε，再查得金屬的標準絕對熵 S(s)，代入S(aq) = S(s) — nFε/ΔT，便可得到離子水溶液的絕對熵。Cu(NO3)2 溫差電池的電解液中若含有1M 或0.5M 的KNO3，電池電壓仍然與溫差成正比， 但卻可獲得較大的電流，我們稱此類溫差電池為「改良型溫差電池」。我們利用改良型溫差電池的原理，自製環保、節約能源、可重複使用的實用溫差電池，以PVC 水管當容器，上、下兩端開口用銅片封住當電極，管內裝海棉及0.125M Cu(NO3)與 1M KNO3 溶液，熱源加熱上層銅片形成溫差，當溫差維持在70℃，電壓約為70 mV，若串聯30 個實用溫差電池，電壓可達2 V 以上，就可以對鉛蓄電池充電。實用溫差電池的熱源可由回收冷氣機、工廠的廢熱，或直接利用太陽能來當熱源。
If the temperature difference cell only goes through the following reaction Then the potential created by the cell is proportional to the temperature difference, and such a reaction purely changes the thermal energy into electrical energy through chemical reaction, which we often name it “typical temperature difference cells”. We can come to the following formula for the typical temperature difference cells through a series of thermodynamic formula: ε= ΔT ． ΔS/ nF (ΔS = S(s)—S(aq), where S is the standard 3 entropy, and n is the number of electrons gained or lost, and 1F = 96487 C). We also provide the following three inferences to demonstrate the special phenomenon for the temperature difference cells: 1. Within the same temperature cell, the electromotive force (EMF) is proportional to the temperature difference. 2. When the temperature difference keeps constant, the electromotive force is proportional to the ΔS in different temperature cells, and is inversely proportional to the number of electrons gained or lost. Within the typical temperature difference cells, when the concentration of the electrolyte becomes more diluted, the concentration of the metal ions also proportionally become lower, which will make the absolute value of the following equation bigger, as a result, will make the electric potential of the temperature difference cells bigger: ΔS = (S(s)—S(aq)) 3. The value of ΔS decides the value of the electromotive force. The ΔS of the following temperature difference cells is positive value: Cu(NO3)2 and ZnSO4 . As a result, within the copper and zinc temperature difference cells, the higher temperature glass is the anode. On the other hand, the ΔS of the AgNO3 temperature difference cell is negative, which means that within the silver temperature difference cell, the higher temperature glass is the cathode. Meanwhile, because the cations and anions can not exist alone, therefore, it is not possible to find the standard entropy of the single ion solution. However, scientists define the standard entropy of the solution containing hydrogen ion to be zero, as a result, we only have to determine the electromotive force for a typical temperature difference cell, while keeping the temperature difference constant, followed by finding the standard entropy for the said metal S(s). Inserting it into the following equation to find the standard entropy for the ion solution. S(aq) = S(s) — nFε/ΔT If the electrolytes for the Cu(NO3)2 temperature difference cell contains 1M or 0.5M KNO3 , the electromotive force is still proportional to the temperature difference, and we can obtain bigger electric current. We call this kind of temperature difference cells “improved version of the typical temperature difference cells”. We try to make more environmental, energy saving, and recyclable temperature difference cell by applying the theory of the improved version of the typical temperature difference cells. We use PVC water pipe as the containers, both edges of the pipe sealed with copper metals, also work as the electrodes. Within the pipe filled with sponge and 0.125M Cu(NO3) and 1M KNO3 solution. The heat source keeps heating the upper copper metal to keep constant temperature difference. When the temperature difference is kept around 70℃, the electric potential is 70 mV. If we can connect 30 practical temperature difference cells in a series, the electric potential will reach 2V, which can then charge the lead rechargeable battery. The heat sources of the practical temperature difference cells can be supplied by the recycled air conditioners, heat waste from a factory, or directly comes from the solar power.
磁場中的離子轉速-探討離子遷移速度變因
帶電的離子受到垂直的磁場與電場作用，會因為受到洛倫茲力而產生有趣的轉動現象。我們利用上述原理設計簡易的裝置設備，探討電解質溶液不同濃度、不同離子電荷數，受到不同離子間靜電力，產生不同的離子移動速度。經由所測量的時間與圓周運動的距離，可計算電解質的絕對遷移速度。由實驗結果推論在固定電場下，當電解質濃度降低，正、負離子間的相互作用力降低，離子遷移速度(migration velocity)加快，莫耳電導率 Λ（mole conductance)也隨之增加。同濃度時，電解質2-1 價型硝酸銅與2-2 型硫酸銅離子強度(ionic strength)不同，2-2型硫酸銅離子強度較大，遷移速度較小，莫耳電導率Λ 也較小。Because of the effect of Lorentz force, charged ion will have interesting rotation under the vertical magnetic and electric field. We use the above principle to design a simple instrument or tool, in order to evaluate and study the formation of different ionic mirgration velocities. The velocity of the charged ion in the instrument is affected by differences in the electrolyte, the charge differences of the ion tested and the differences in electrostatic forces between ions. From the experiment we can deduct that at a fixed constant electric field, when the concentration of the electrolyte is reduced, the interaction of forces between positive and negative ions will be reduced. When the migration velocity of ions increase, the mole conductivity Λ (mole conductance ) will also increase. At the same concentration, the ionic strength between copper nitrate ( 2-1valency type ) and copper sulfate ( 2-2 valency type ) are not identical. Copper sulfate, a 2-2 valency type has higher ionic strength, the velocity is slower and the mole conductivity Λ is also smaller.
奈米複合材料與空氣分子的愛恨情仇-探討奈米碳管對空氣滲透率之影響
本實驗使用聚醚亞醯胺溶液製備基本薄膜，由於玻璃態高分子薄膜過於緻密，一直是高分子薄膜在應用上的一大限制，為了在薄膜上製造缺陷，又不會使薄膜之選擇性降低，因此選擇將酸化之奈米碳管(孔徑10~20nm) 加至聚醚亞醯胺薄膜中。本實驗主要為探討添加不同濃度的酸化奈米碳管對聚醚亞醯胺薄膜的滲透率與選擇率的影響，藉由添加0.5 wt%、1 wt%、1.5 wt%、3 wt%、4 wt%等不同濃度的酸化奈米碳管至15 wt% 的聚醚亞醯胺溶液中，並製作薄膜，測試其基本性質與五種氣體(H2、CO2、O2、N2、CH4)的滲透率及不同空氣分子之間的選擇率。我們總共測試了三種薄膜的性質，分別是表面特性、熱穩定性及結晶型與層間距，薄膜的表面性質，能觀察到奈米碳管在薄膜中製造奈米孔隙結構，增加氣體滲透的孔道，能有效增加氣體的滲透率。增加奈米碳管的量，能有效升高第一階段熱裂解的溫度，雖然熱裂解在本實驗中沒有很大的差異，但是還是可以從熱重分析圖中推測不同量的奈米碳管會影響熱穩定性。在X 光繞射實驗中，添加奈米碳管的薄膜與純聚醚亞醯胺薄膜，在結晶相上都屬非結晶型薄膜，添加了奈米碳管的高分子複合薄膜的層間距明顯增大。在氣體滲透實驗中，我們比較了不同氣體或濃度不同的奈米複合薄膜的氣體滲透率，在不同氣體時，氣體的滲透率會隨著奈米碳管濃度增加有明顯的提升，五種氣體滲透率大致依照H2>CO2>O2>N2>CH4 這個趨勢增減。奈米碳管對1.5%增加到3%或4%的奈米複合薄膜滲透率的影響卻減小，由此可以推斷奈米碳管對空氣滲透率並非無限制的增加，在1.5%以後就漸漸趨近最大值。H2為14.89barrer，CO2 為9.51barrer，O2為6.34barrer，N2為6.48barrer， CH4 為3.75barrer 。本研究總共比較了三組氣體的選擇率，分別是CO2/CH4，O2/N2，H2/CH4，分離率最高的是H2/CH4 的，兩分子的粒徑大小差對分離率有極大影響，差愈大，其分離率也愈高。奈米碳管的量改變並不會使薄膜的氣體選擇率明顯增加或減少，但是加入太多奈米碳管其選擇率會變低。在五片薄膜中，1.5%的薄膜有最好的選擇率，奈米碳管的添加量超過1.5%選擇率就會開始下降。綜合滲透率及選擇率可以分析出，添加1.5%奈米碳管的高分子奈米複合薄膜有較高的滲透率，又不會降低選擇率，在利用上比其他濃度的奈米複合薄膜在有害氣體過濾及空氣的分離回收方面產生更好的效果。;This experiment uses Polyetherimide polymers solution to make basic membranes. Because glassy polymer membranes are too dense for gas permeations, it is one of the limitations in their applications. To increase gas permeability and maintain air selectivity, I made some nanogaps on the surface of the membranes by an acidification multi-wall carbon nanotubes (MWNTs, kinetic diameter 10~20nm) in the PEI membranes. We mainly want to find if it has some influence between the consistency of acidification MWNTs and gas permeability or selectivity. We mixed 0.5wt% 、1wt%、1.5wt%、3wt%、4wt% acidification Carbon nanotube in 15wt% PEI solution, made membranes and tested the character, five kinds of gas permeability (H2、CO2、O2、N2、CH4) and the selectivity between different gases. We have tested the three nature of membranes, including surface characteristic, TGA and XRD. We can see some nanogapes made by carbon nanotube in the membranes. It could availably increase gas permeability. Mixing more carbon nanotube in the membranes could increase the temperature of the first heat-decomposition. Though the heat-decomposition in this experiment didn’t change a lot, we could say that different percent nanotube would affect the membranes’ heat-decomposition. By the experiment of XRD, the membranes with carbon nanotube and the pure PEI membranes attach to amorphous membranes. Nanocomposite’s de-spacing is bigger than pure membranes. In the experiment of air permeability, we compared different kinds of gas or different percent carbon nanotube of nanocomposite if they have some change of permeability. The conclusion is that air permeability increase as the quantity of nanotube increase. The five kinds of permeability the direction：H2>CO2>O2>N2>CH4.The influence of permeability will decrease when the quantity of carbon nanotube increase from 1.5% to 3% or 4%. We can get the conclusion that the increment of gas permeability isn’t limitary. It drifts towards maximal about 1.5%. H2 is 14.89barrer. CO2 is 9.51barrer. O2 is 6.34barrer. N2 is 6.48barrer. CH4 is 3.75 barrer.This experiment totally compared three groups of air selectivity. They ’re CO2/CH4, O2/N2 and H2/CH4. The maximum of selectivity is H2/CH4. The difference of kinetic diameter affects air selectivity a lot. The quantity of nanotube doesn’t associate with the air selectivity, but mixing too much nanotube will decrease air selectivity. The 1.5% nanocomposite has the highest selectivity. If the consistency of the membranes is higher than 1.5%, the air selectivity will decrease. Depend on the gas permeability and the air selectivity, the 1.5% nanocomposite has higher permeability and constant air selectivity. That shows the 1.5% nanocomposite has a better effect on air selectivity and recycling.