全國中小學科展

動物學

蚊幼蟲與搖蚊幼蟲在水中分布與其血紅蛋白基因表現之關係

蚊蟲呼吸透過氣管系統,本研究假設底棲孑孓與搖蚊幼蟲具備血紅蛋白系統以擷取氧氣。戶外採集8種孑孓和1種搖蚊幼蟲,錄影觀察白腹叢蚊、竹生翠蚊和鹽埕搖蚊幼蟲長時間停留底層;家蚊、斑蚊則反覆上下至水面呼吸。顯微鏡下發現白腹叢蚊幼蟲體壁內側充滿紅點,色澤隨著富氧或缺氧而變化;然二種斑蚊則不明顯。PCR增殖6種蚊蟲和1種搖蚊血紅蛋白基因片段並建構親緣關係樹。定量RT-PCR顯示,白腹叢蚊與鹽埕搖蚊在缺氧條件下,血紅蛋白相對表現量分別增加5倍和150倍,上層埃及斑蚊和白線斑蚊則增加3.9倍和1.5倍。西方墨點法證實孑孓缺氧條件下,17-kDa血紅蛋白皆有大量表現。本研究首次確定6種蚊蟲具血紅蛋白基因,證明孑孓血紅蛋白基因在缺氧下的表現量增加,包括mRNA與蛋白質。此等反應氣候變遷,暖化導致水溫升高與低溶氧條件下,蚊蟲可能有因應環境改變的呼吸機制。未來將進一步標幟血紅蛋白在蚊幼蟲體內組織之表現與其他環境變因之影響。

The Reproduction success of the Cyprinidae and a Claridae fish species and its impact on small- scale fisheries

To investigate the reproduction success and natural recruitment of several Cyprinidae fish and Claridae fish species in the Allemanskraal Dam. The purpose of the project included investigating whether each individual fish species studied has a successful 2020/2021 spawning season in comparison with each other. Sections of the seine net were measured along with a distance of 10 along the shoreline. The ends of the seine nets were attached to one foot and the top of the net was held by hand. Both volunteers moved in unison while covering the 10m. The volunteer in the “deep end” moved towards the shoreline creating a semi-circle while the other volunteer remained stationary. The two ends of the net were then pulled onto the shore and the fish were collected. The results found that the Labeo Umbratus and Cyprinus carpio had the most successful spawning seasons with the highest recorded numbers. These high numbers of the Labeo Umbratus can be due to the fact that the species lays a large number of eggs. The high numbers of the Cyprinus carpio is due to the lower numbers of the other fish species as previous studies have shown that the species negatively impacts the environment which could in turn negatively impact the other fish species. The Claridae gariepinus and Labeobarbus aeneusas were the lowest. The low numbers of the Labeobarbus aneusas may be due to their slow growth and late maturity rate. The Labeo capensis had an average number relative to the other species and this is due to the fact that during the sampling period the dam was at 100% capacity as this is essential for the survival of the juvenile fish. The hypothesis was accepted as the Labeo Umbratus, Cyprinus carpio and Labeo capensis all have a successful spawning season. However, due to the size of the Cyprinus carpio, they would be most suited for a small scale fishery.

台灣藍鵲的習性與領域行為之研究

近年來都會區常有臺灣藍鵲出沒,讓習於都市文明的現代人不知如何應對。本研究目的在了解臺灣藍鵲的習性、合作生殖行為、領域行為,進而探討引發臺灣藍鵲威嚇、攻擊行為的因素。從民國106年2月起至111年9月止,在住家庭院前和社區進行有系統的生態習性觀察 ,透過拍照、錄影,最後實驗出顏色、距離、聲音是否影響臺灣藍鵲領域行為。結果發現:繁殖期是從三月上旬開始,七月上旬結束。當發現牠們開始築巢時,不要靠近或干擾,因為臺灣藍鵲有領域行為,要距離臺灣藍鵲至少54.4公尺以上,遠離鳥巢7.1公尺範圍以外,也要避免穿紅色衣服或桃紅色衣服在鳥巢樹下走動,才不會引起臺灣藍鵲出現威嚇行為。不要讓聲音超過 97 ±2(mean S.D.)分貝以上,那是臺灣藍鵲無法忍受的噪音範圍。所以,與臺灣藍鵲就地保育與共存,讓人們認識、理解牠,是減少衝突的關鍵。

The Reproduction success of the Cyprinidae and a Claridae fish species and its impact on small- scale fisheries

To investigate the reproduction success and natural recruitment of several Cyprinidae fish and Claridae fish species in the Allemanskraal Dam. The purpose of the project included investigating whether each individual fish species studied has a successful 2020/2021 spawning season in comparison with each other. Sections of the seine net were measured along with a distance of 10 along the shoreline. The ends of the seine nets were attached to one foot and the top of the net was held by hand. Both volunteers moved in unison while covering the 10m. The volunteer in the “deep end” moved towards the shoreline creating a semi-circle while the other volunteer remained stationary. The two ends of the net were then pulled onto the shore and the fish were collected. The results found that the Labeo Umbratus and Cyprinus carpio had the most successful spawning seasons with the highest recorded numbers. These high numbers of the Labeo Umbratus can be due to the fact that the species lays a large number of eggs. The high numbers of the Cyprinus carpio is due to the lower numbers of the other fish species as previous studies have shown that the species negatively impacts the environment which could in turn negatively impact the other fish species. The Claridae gariepinus and Labeobarbus aeneusas were the lowest. The low numbers of the Labeobarbus aneusas may be due to their slow growth and late maturity rate. The Labeo capensis had an average number relative to the other species and this is due to the fact that during the sampling period the dam was at 100% capacity as this is essential for the survival of the juvenile fish. The hypothesis was accepted as the Labeo Umbratus, Cyprinus carpio and Labeo capensis all have a successful spawning season. However, due to the size of the Cyprinus carpio, they would be most suited for a small scale fishery.

The Population Structure of the Orange River mudfish (Labeo capensis) in Allemanskraal Dam and Its potential as a Fishery Species

The aim of this research was to investigate whether the ecology and biology of the Orange River mudfish Labeo capensis were suitable for the species to be used in fisheries. Three fleets of the gill nets were set, parallel to the shore. One fleet was lifted, and the fish were collected by hand. The two remaining fleets were lifted the next day. The seine net was pulled for 10 metres within the littoral zone. The net was then pulled towards the shore of the dam and the procedure was repeated four times. The four fyke nets were set parallel to the shore and were left for two nettings nights and then lifted. All fish caught were collected by hand and placed into buckets. The majority (82.93%) of the fish caught were within the 0-100 mm size class. The 101-200mm and 201-300mm size classes contain similar numbers of fish, while no fish were caught in the 301-400mm size class. The hypothesis was accepted. Allemanskraal Dam, as of the study period, has a very small juvenile fish population of L. capensis, as only 7 out of 41 fish individuals caught were within the 101- 300mm fork length size class. These results show that the population of L. capensis is not established as of yet, as the research did was right after their breeding season. Historical research has shown that sexually mature individuals of the L. capensis species tend to be a minimum of 300mm SL, 4-6 years after hatching. The population was largely young-of-the-year and may develop into an established population in 3-4 years (after sexual maturity). The L. capensis population in Allemanskraal Dam has the potential to be a fishery species if suitable conditions are maintained. Establishing this species’ potential will therefore allow economically viable fisheries to utilise them sustainably and to their full economic potential.

The Population Structure of the Orange River mudfish (Labeo capensis) in Allemanskraal Dam and Its potential as a Fishery Species

The aim of this research was to investigate whether the ecology and biology of the Orange River mudfish Labeo capensis were suitable for the species to be used in fisheries. Three fleets of the gill nets were set, parallel to the shore. One fleet was lifted, and the fish were collected by hand. The two remaining fleets were lifted the next day. The seine net was pulled for 10 metres within the littoral zone. The net was then pulled towards the shore of the dam and the procedure was repeated four times. The four fyke nets were set parallel to the shore and were left for two nettings nights and then lifted. All fish caught were collected by hand and placed into buckets. The majority (82.93%) of the fish caught were within the 0-100 mm size class. The 101-200mm and 201-300mm size classes contain similar numbers of fish, while no fish were caught in the 301-400mm size class. The hypothesis was accepted. Allemanskraal Dam, as of the study period, has a very small juvenile fish population of L. capensis, as only 7 out of 41 fish individuals caught were within the 101- 300mm fork length size class. These results show that the population of L. capensis is not established as of yet, as the research did was right after their breeding season. Historical research has shown that sexually mature individuals of the L. capensis species tend to be a minimum of 300mm SL, 4-6 years after hatching. The population was largely young-of-the-year and may develop into an established population in 3-4 years (after sexual maturity). The L. capensis population in Allemanskraal Dam has the potential to be a fishery species if suitable conditions are maintained. Establishing this species’ potential will therefore allow economically viable fisheries to utilise them sustainably and to their full economic potential.

全臺新記錄種皺家蟻特徵、習性及其偷懶行為之研究

本研究藉由觀察及設計掉落式陷阱分析校園內螞蟻生物多樣性,發現校園螞蟻種類多達12種,而其中「新皺家蟻」經專家鑑定為全臺新記錄,而其特徵與同屬螞蟻不同處為觸角節數,「新皺家蟻」為11節,其餘皆為12節;而前伸腹節刺與同屬蟻種均不相同,「新皺家蟻」及日本皺家蟻皆為一巢多后制,前者依營養需求轉換進食對象,後者偏好肉類蛋白質。在養殖時發現其反抗能力相對於同屬蟻種差,且工蟻大多時候都在偷懶,深入探討後發現牠們是在等待任務,但當積極工蟻疲勞時或危急情況發生時,承當替補或應對者。由於偷懶工蟻在特定情況下變得積極,故以「行為次數」進行分析比「偷懶工蟻比例」更能呈現工蟻的偷懶程度,新皺家蟻偷懶行為發生率約84%,偷懶工蟻比例約40%。工蟻越接近死亡越不偷懶,但偷懶行為不受族群組成影響。而雄、雌蟻只負責延續族群。

在壓力下更要堅強,探討ACE2/Ang(1-7)軸與韌力的關係

背景:韌力 (resilience)指個體遭受壓力時快速調適及復原的能力,是許多疾病的保護因子但生理機轉仍不明。Angiotensin-converting enzyme 2/Angiotensin(1-7) [ACE2/Ang(1-7)]軸具有保護心血管、抗焦慮及保護神經的潛力。目前仍無ACE2/Ang(1-7)軸與韌力相關的研究。另外ACE2是SARS-CoV-2病毒進入人體的受體,相關研究在疫情下更顯重要。 目的:本研究中我們以習得無助行為模式篩選具韌力行為的大鼠(resilient, R),探討韌力鼠與非韌力鼠(non-R) ACE2/Ang(1-7)軸表現的差異。 假說:韌力鼠的ACE2/Ang(1-7)軸活性較非韌力鼠高。 結果:韌力鼠在壓力下其corticosterone (CORT)濃度與控制組無差異。韌力鼠的Ang(1-7)濃度較非韌力鼠及控制組高,暗示較高的Ang(1-7)濃度與韌性表現相關。以Ang(1-7)/Angiotensin II (Ang II)比率作為反映ACE2 轉化Ang(1-7) 功能的指標,發現韌力鼠的ACE2濃度及功能均與未主動逃跑而受電擊的時間相關,暗示其ACE2活性能因應壓力調控上升。韌力鼠的ACE2濃度雖較非韌力鼠低,但其ACE2功能則較其他兩組高。並且,Ang (1-7)濃度及ACE2功能僅在韌力鼠與ACE2濃度呈現正相關,但在非韌力鼠則無相關。暗示在壓力下韌力鼠的ACE2仍維持有效率的功能但非韌力鼠的ACE2功能則受影響。另一方面,Ang (1-7)濃度僅在非韌力鼠與CORT濃度負相關,暗示非韌力鼠個體的ACE2/Ang (1-7)]軸活性與海馬迴-下視丘-腦下垂體(HPA)軸的活性可能相關。 結論:韌力個體的ACE2/Ang (1-7)軸活性較非韌力鼠及控制組佳且可能因應壓力而調節增加。相反的,非韌力鼠個體的ACE2/Ang (1-7)軸功能較差且可能與HPA軸的活性相關。我們的研究顯示ACE2/Ang (1-7)軸扮演調節韌力的重要角色。ACE2/Ang (1-7)軸活性是否可以做為個體韌力的指標或具有增進韌力的臨床價值需進一步研究。

Super「鼠」跑

透過文獻探討已知高血壓會引起腦發炎,導致腦功能受損。研究結果也顯示運動能增進腦部功能。因此,在此實驗中,我們想要探討高血壓引起的腦發炎和功能退化等問題能否透過運動改善。將鼠分為 2K1C 手術誘發高血壓組(2K1C)及沒有高血壓(Sham)的對照組,並再各分為運動組(Sham-EX、2K1C-Ex)和不運動組(Sham、2K1C)。小鼠腦部切片中微小膠細胞數量顯示 2K1C 的小鼠組別腦發炎最為嚴重,且 HE 染色發現一樣為 2K1C 小鼠組別的神經細胞數目最少。接著我們以莫里斯水迷宮測試小鼠學習、記憶功能,結果 Sham-Ex 及 2K1C- Ex 尋獲平台時間皆有減少趨勢,而 2K1C 尋獲平台花費最長時間,顯示運動確實可以減緩高血壓造成的腦功能受損。

魚類在面對酸逆境下醣類的代謝差異

溫室氣體產生造成環境變遷,使得水質酸化,進而影響生態系統。生存在水中的魚類,為了適應水質的酸化,他們的生理上必定有一些變化。為了找出魚類適應酸化環境所做出的改變,我們選擇以斑馬魚及青鱂魚做為實驗動物以研究水質酸化對於其代謝之影響,分析其中與能量代謝及合成相關的器官,包含消耗大量能量的以維持酸鹼平衡的鰓、能量供應相關的肝臟和肌肉。綜合即時聚合酶連鎖反應及免疫螢光染色的結果,我們發現斑馬魚在面對酸性環境下時醣類代謝的情形是有改變的,肝臟內肝醣分解及糖質新生的效率提升,且鰓部位醣類代謝效率提升,推測是運送葡萄糖給鰓部位進行排酸代謝,其鰓部位細胞內的酵素也因此出現了細胞分工的現象。而青鱂魚在面對酸性環境下時,透過肝醣分解提供更多的葡萄糖進行糖解作用,同時抑制了糖質新生反應效率,推測其演化出了高效率的排酸方法。相較於斑馬魚,青鱂魚醣類代謝不會產生過多的能量,也意味著青鱂魚能量使用效率有提升。