Arabidopsis thaliana bZIPs（AtbZIPs）是一群影響層面相當廣泛的轉錄因子，一半以上的 AtbZIPs 基因表現受到光的調控，且近九成的分子機制尚未明瞭，因此探討 AtbZIPs 在植物光調控機制中所扮演的角色將是個有趣的課題。AtbZIP16 與 AtbZIP17 皆被推測會參與光的調控機制，然而迄今少有文獻針對這二個轉錄因子進行更多的研究。因此，我們想藉由細菌單雜合系統（Bacterial one hybrid system）的方法，找出能與 AtbZIP16 與 AtbZIP17轉錄因子結合的 DNA 序列，以瞭解此二轉錄因子調節下游基因表現的分子機制，並探討其在光訊息傳導途徑中所扮演的角色。針對 AtbZIP16 與 AtbZIP17，本實驗分別找到了 7 與10 種可能的結合序列。首先，經由資料庫比對分析，我們發現其序列上帶有的 motifs 功能，主要參與在光調控、環境逆境反應機制、組織發育、賀爾蒙調節、病原菌防禦、鈣離子訊息傳遞等方面，其中又以光調控佔最大的比例。再者，藉由將 motifs 的功能繪製成文氏圖，並與 HY5 （AtbZIP56）做比較，結果顯示，這三個轉錄因子雖同屬於 AtbZIP family，據推測皆受到光的調控，可能參與某些相似的生理調節過程，但都各自具備不同的功能，影響植物體的發育。如此的差異，表示他們有實質上的不同，值得我們更深入的研究。整體而言，本實驗結果除了說明 AtbZIPs 的功能確實廣泛之外，也顯示AtbZIP16 與AtbZIP17 是執行光訊號傳導很重要的調控因子。Arabidopsis thaliana bZIPs (AtbZIPs) is a group of transcription factors affecting a wide range of responses in Arabidopsis. The expression of more than half of the AtbZIPs is regulated by light, and the molecular mechanism for roughly 90% of these AtbZIPs remains unknown. Therefore, the roles AtbZIPs play in Arabidopsis light signal transduction is an interesting topic to pursue. AtbZIP16 and AtbZIP17 have been suggested to participate in the regulation mechanism mediated by light. However, only limited studies for these two transcription factors have been previously performed. For this reason, we intended to determine the DNA-binding sequences for AtbZIP16 and AtbZIP17 via the bacterial one hybrid system to reveal their target binding sites in the promoter region of their downstream genes and to speculate their possible biological function especially in light signal transduction pathway. We have identified 7 and 10 possible recognition sequences for AtbZIP16 and AtbZIP17, respectively. Using motif-finding programs analyses, we found the motifs identified are mainly involved in light and stress signaling, tissue development, hormone regulation, pathogen defense and Ca2+ signaling. Among these regulation pathways, sequences involved in light regulation owns the highest proportion. Furthermore, a Venn diagram was generated to compare functions of genes regulated by AtbZIP16, AtbZIP17 and HY5. Results revealed that, although these three transcription factors all belong to the AtbZIP family and are predicted to be regulated by some similar physiological regulation process (e.g. light), they still possess distict biological functions in plant development. Further studies are thus required to put these transcription factors into their shared and unique biological context. Taken together, the results of this experiment not only indicated light is a key regulation factor for AtbZIP16 and AtbZIP17, but also showed the function of AtbZIPs could be diverse.

a. Purpose of the Research Nowadays people are getting unhealthy, especially the heart. Since the outbreak of SARS, the Hong Kong citizens cared more about their health. In the past two decades, due to the technological advancement, many medical instruments that were used by doctors are now available to the public. One of the examples is the sphygmomanometer used for measuring blood pressure. On the other hand, very few heart monitoring devices are developed for public use. As a result, there is a need that such heart monitors should be available to the public. b. The device Our device is a modified stethoscope, which electronic components are added to this common medical instrument. The device mainly consists of 3 parts: 1. The sensor: modified from ordinary stethoscope, which a condenser microphone is added to change the heart sounds into electrical signals 2. The signal processor: integrated circuits and resistor-capacitor couples, which the cost is much lower than digital electronic components, are used to amplify and filter the noise in the electrical signals. The processor is divided into 3 stages: - Preamplifier: Amplifies the electrical signals converted from heart sounds - Low pass filter: Filters the noise in the signal. The cut-off frequency is 600Hz, which most of the heart sounds are below 600Hz. - Power amplifier: Amplifies the filtered signals before outputting into computers or earphones. 3. The output devices: it can be a computer or an earphone. In a computer, the heart sounds can be converted into graphs, enabling precise graphical analysis. Since many abnormal heart conditions will alter the heart sounds, with the aid of computers and graphs, people can know whether their hearts are normal or not, and can seek for medical support before developing any critical situation. Moreover, abnormal heart sounds are more significant in graphs, so any heart problems can be discovered more easily. c. Data During an exhibition in Hong Kong, about 1000 people tried the device. Among them approximately 150 people were confirmed to have heart problems with abnormal heart sounds. Using our device, we discovered 109 of them. As a result, the accuracy of the device is about 72.6% d. Conclusion With the low cost of our device (~€9.80/US$12.80), everyone would be able to afford it. As a result, people can check their conditions of their hearts more frequently, and would be able to discover any early heart problems.

This research analyzes goldfish’ (Carassius auratus) environmental preference behavior by setting up different environmental color-models to spur goldfish’reactions. It is investigated that pre-conditioned preference of goldfish was stressed by electric shock or high osmotic pressure. We’re interested in how stress experience modulate goldfish’ exploring behavior and its environmental preference. Goldfish that prefers brown-colored environment (compared with white) is suitable for being an model animal to investigate fish’s environmental preference. Both electric shocks and high osmotic pressure, two different kinds of stress, can affect goldfish’ behavior. It shows that fish have the ability to learn and memorize the stress experience. Besides, different stress has different effects on fish’ environmental preference behavior. It proves that there are complicated interactions among environmental stimulus, memories of stress and behavior caused by different kinds of stress.本研究利用環境色彩特徵的差異，建立朱文錦(Carassius auratus)環境偏好行為之動物模式，探討不同逆境刺激經驗(施予電擊或高滲透壓環境)，與魚類環境偏好行為的交互作用，以研究魚類的逆境經驗，是否可形成記憶，並影響其行為。我們發現朱文錦偏好土色環境(相對於白色環境)，適合作為探討魚類對環境偏好的實驗動物。電擊與高滲透壓兩種不同性質的逆境經驗，皆可影響朱文錦的環境偏好，顯示魚類具有記憶逆境環境的學習能力。此外，不同性質的逆境經驗，對魚類的環境偏好行為具有不同的影響。證明「環境刺激—經驗(記憶)—行為表現」三者之間具複雜的交互作用。

In this research we derived heating effects on anti-oxidation by optical rotation analyses. A simple and accurate Polarimeter was devised with polar screens, laser, and photo resistor; the laser was used for light source, and the photo resistor for detection. Four kind of fruits, Hyloceeus polyrhizus, California plum, kiwi and tomato were experimented by heating them in boiling water and microwave oven. Their optical rotations versus time duration were calculated. Then we used ABTS/ the H2O2/HRP analysis system from Arnao's research to obtain the heating effects on anti-oxidation by calculating the vitamin C densities versus lag time. We found that there was a tendency of increased anti-oxidation at the beginning by heating, but decreased afterwards. Better oxidation was achieved by using microwave for Hyloceeus polyrhizus, by using boiling water for kiwi and tomato, and by using both methods for California plum. The same tendency was also found in the optical rotation analyses. Therefore we successfully developed a new method to measure the antioxidant activities by the optical rotation. We can also apply this method to simulate the fruit digestion process in the stomach, which let us understand further about anti-oxidation ( or optical rotation ) versus time. 本研究利用偏振片、雷射光為光源、光敏電阻為偵測器，組裝成一個簡易且精確的旋光度計。 我們利用此旋光度計對奇異果、火龍果、聖女蕃茄、加州李子四種水果以微波、水煮及油浴三種方式來處理，求出水果的旋光度與熱處理時間的關係。再藉由Arnao 研究的ABTS/ H2O2/HRP 分析系統，以不同濃度的維生素C與延遲時間畫圖作為標準曲線來測量總抗氧化活性，並比較熱處理後抗氧化活性的變化趨勢。 透過本研究可以了解不同熱處理方式對四種水果之抗氧化活性初期皆有增加的趨勢，但隨著時間增長則抗氧化活性降低。其中奇異果與聖女蕃茄用油浴，火龍果用微波處理，加州李子則是用任何熱處理方式均可得較佳的抗氧化活性，而熱處理後在旋光度測量上也有相同的趨勢。因為此兩種方法有一致的反應趨勢，所以證明我們成功地開發出一種可以利用旋光度來測定抗氧化的新方法。 最後，應用本方法進一步模擬水果在胃部裡的消化，讓我們更可以了解在整個消化的過程中抗氧化活性(旋光度)隨時間變化的情形。

秀麗隱桿線蟲(Caenorhabditis elegans)為在科學界備受矚目的模式生物，對於其生殖及發育過程已有諸多研究，然而對於其覓食及攝食行為的相關研究卻仍闕如；每一種生物都有其獨特的行為模式，而為了解線蟲發展出何種覓食與攝食的行為以適應自然的環境，因此著手進行研究。本研究歸納出線蟲的七種一般行為：前進、探頭、偏移、擺頭、後退、轉向及拐彎行為，並探討其意義。其中，前進及探頭行為為恆常出現。偏移與轉向行為與攝食有關，在有菌環境中出現，可避免其遠離食物來源，而連續的後退及轉向行為也構成特殊行為之樹枝狀行為，推測應為幫助線蟲的消化所出現的行為。擺頭與拐彎行為與覓食有關，在無菌環境中出現。後退行為雖在有無菌環境中皆出現，但由後退後行為的不同可以了解後退行為在兩種環境下具有不同意義。在有菌環境中，後退後通常伴隨轉向行為；在無菌環境中，後退後通常伴隨拐彎行為，為逃離不適環境且藉以覓食的行為。經過研究，發現線蟲的覓食機制為嗅覺，且對嗅覺有依賴性及專一性，在其他氣味存在的環境下會大大降低覓食的效率，但最後仍然能正確地找到食物來源。線蟲於覓食的過程中所出現的特殊行為之混亂行為，由實驗結果發現為是E. coli 所釋放至培養基的化學物質所造成，化學物質濃度越濃，線蟲所表現出的混亂行為就越明顯。推測此種現象發生的原因，應為E. coli 釋放化學物質以干擾線蟲，以避免本身受到捕食，然而，線蟲也演化出適應的能力，因此在發生混亂行為後仍然能抵達菌落，此種特殊的交互作用表現出線蟲與E. coli 間的共演化現象。Cenorhabditis elegans has long been a model organism used for developmental biology studies. However, researches about its foraging and feeding behaviors are still limited. Every organism has its unique behaviors, and this research was conducted in order to understand more on what kind of foraging and feeding behaviors were developed by C. elegans and how they helped C. elegans to adapt to the environment in which it lives. The research concluded with seven general behaviors of C. elegans : advancing, probing, slanting, swaying, backing, turning and bending, and the meanings of it. The advancing and probing behaviors are constant. The slanting and turning behaviors, which appear in presence of food, are feeding-associated behaviors, which help C. elegans avoid leaving the food source. Continuous backing and turning behaviors also make up the branching behavior, one of the special behaviors, which help C. elegans’ digestion. Swaying and bending behaviors, which appear in absence of food, are foraging-associated behaviors. Although backing behavior appear both in presence and absence of food, the difference in post-backing behaviors show that there are distinct meanings of backing behavior in each environment. In presence of food, turning after backing is usually observed; in absence of food, bending after backing is usually observed, which indicates the act of escaping from unsuitable environment and searching for food. Studies show that the foraging mechanism of C. elegans is by olfaction, and C. elegans is highly olfaction-dependent and specific: efficiency of foraging decreases significantly under environment with scents other than that of E. coli, but C. elegans is still able to locate the food. According the experiments, the chaos behavior, one of the special behaviors, appears during the foraging process of C. elegans, and is caused by chemicals released to the medium by E. coli. The density of the chemicals and the extent of chaos behavior is in direct proportion. The theory is E. coli secrets chemicals to disturb the worm to avoid being preyed upon. C. elegans, however, also evolved the ability to adapt to the chemical so that it can still reaches the food source even after chaos behavior appeared. This unique interaction is a manifestation of co-evolution between C. elegans and E. coli.

「風笛」是台灣原住民鄒族的信號用具及祈雨法器，由一條繩子綁一支竹片構成。轉動風笛時，竹片會繞繩子自轉並拍打空氣而發出聲音，並有上下飛舞的現象。風笛產生聲音的原因，為竹片拍打空氣而造成的渦流共振現象；又由於繩子扭力大小及方向改變，使風笛的音調忽高忽低、響度忽大忽小、且竹片會在兩個平面上公轉，而有週期性變化。施力使風笛公轉轉速加快時，竹片自轉速率也變快，使其音調愈高、響度愈大；而繩愈短、愈粗時，竹片的公轉週期將愈短。The wind whistler was once used by Tsou aborigines as a tool for message transfer. It is composed of a string and a bamboo flapper. When swung around, the flapper spins, beats the air, and makes sounds. Moreover, the flapper flies up and down during the revolution. The spinning flapper beats the air, causes the vortex resonance phenomenon, and thus produces sound. As the twist torque and direction change, there is periodical variation in the sound volume, sound pitch, and the movement of the flapper, which orbits up and down at two planes. If given force to speed up its revolution, the flapper，s spinning frequency also increases, which makes the sound pitch higher and the sound volume greater. Besides, when the string is shorter or thicker, the flapper，s revolution period will be shorter.

BCH (Bnip-2 and Cdc42GAP homology) domains are a novel class of protein-interacting domains. First identified in BNIP2 and in Cdc42GAP, a GTPaseactivating-protein (GAP), canonical BCH domains play important and varied roles in the control of cell morphology and apoptosis induction, amongst others. However, this domain is currently classified under lipid-interacting sec14 domains due to low sequence identity (~20%). Given this intriguing similarity, the relationship between BCH and sec14 domains is worth further study. As BCH/sec14 domains are closely associated with cell signaling and human disease, characterization of these domains and the elucidation of their functional diversity could better aid our understanding of their cellular functions and disease pathogenesis.

Starting from the problem in AMC competition of Australia, we try to find out the locus and its length when a point in a regular polygon rolls in a circle. The result is that the locus has a wonderful and regular cycle.Next, we discuss the regularity of the cycle when a regular polygon（n sides） rolls in another regular polygon. Furthermore,we discuss the the equation of the locus by changing the radius and the angle of rolling. we find out the argument function of the locus of a point inside when a a regular polygon（n sides）rolls in another regular polygon （m sides）： ， Aj is the summits of the regular polygon（m sides）, Bjcorresponds Aj when a point inside the regular polygon (n sides) rolls, ) And then, we do some moving simulation with some computer math software, such as Cabri Geometry、Mupad, etc. We discuss the regularity of the locus and its equation of a point inside when some special cycloids, like asteroids, cardioids, etc, roll in a certain condition. Moreover, with the result of research 2, we create the “plate＂ and apply for a patent on it. We hope to study math by playing games. 從澳洲AMC 競賽題出發，嘗試探討一正n 邊形中的一點在單位圓內滾動軌跡及其軌跡長度，發現該軌跡均會產生奇妙的循環規律。 接下來，推廣探討正n 邊形在其他正多邊形中滾動時循環的規律，並利用旋轉半徑及角度之間的變化深入探討其滾動軌跡方程式，發現正n 邊形繞正m 邊形滾動時其內部一點軌跡參數式為，其中， Aj 為 正m 邊形之各頂點、Bj 為正n 邊形中內部一點旋轉時對應 Aj 之點，。 進一步想嘗試使用數學電腦軟體如：Cabri Geometry、Mupad 等對以上研究去做一些動態模擬，並再探討一些特殊擺線如：星狀線、心臟線…等，在條件下相切滾動時，圖中某一點的軌跡規律性及其方程式。另外，應用研究二中的結果，創造出寓數學於遊戲的「圖形板」，並申請了新型專利。

The reason for making this project was with the intention to help the blind persons. A lot of people with vision problems don’t have the resources available to acquire equipment or programs that make it easier for them to use a computer to write, to communicate etc... Being aware of this problem we took the liberty of researching at a community center that helps the blind persons in Tijuana Baja California, Mexico. We found that they had a type writer (Perkins) with only 6 keys that symbolized Braille. They also had a normal keyboard that had the Braille code over each key, but it is complicated to learn because of its many keys. Due to our research our intention was to design a system that respect the Braille symbols of the Perkins type writer and translated them to the common alphabet. We believe that this project will help many people especially the blind, with writing books, letters, articles, etc. So they can be integrated to activities and jobs reserved, until now, for people with the sight sense. My project also has 8 keys, when the person press a key combination the PC emits the sound of the letter. And when a word is completed the PC reproduce the whole word on the speaker. When the entire paragraph is completed there is an option to reproduce it. It has also other functions as erase everything, back space, etc. All these with the 8 keys. To control the system I used the parallel port of a PC, with a control program made in Visual Basic. Currently there are systems for the blind but are very expensive, they have a cost of approximately $900.00 US dollars, which is a high price to pay for some people. My project has a cost of $70.00 US dollars thus making it easy for a person who has a computer to be able to buy this keyboard. This project is being use in the “Blind Persons Training Center” of Tijuana city. They helped us to develop it, giving some ideas to improve it.

本專題研究之目的，在於運用簡單的原理自製出準確、廉價、適用於低風速範圍的風速計；方法是藉由將一連接轉軸的玻璃片迎風，探討迎風時，玻璃片偏轉夾角和風速之間的關係。實驗過程中同時發展出可以用馬達旋臂的裝置與v=rω的原理，產生穩定可計算風速的環境。研究結果發現：在低風速的環境下，θ 範圍小時玻璃片與地夾角和風速大略成正比的關係，所以我們可以得到近似式” v≒aθ+b”，參數(a,b)依玻璃片而定。另外θ 範圍大時，我們可以使用二次逼近曲線的方程式與內插法來作角度與風速的對應。藉由控制玻璃片的厚度、質量與形狀，我們可以製造出相對應風速範圍的風速計。The purpose of our research is to use easy theorems to make accurate and inexpensive anemometer which is available in low wind velocity. To make such anemometers, we made a piece of glass, which was connected to a axle, to face wind; then, we could figure out the corresponding relation between wind speed and the angle created by the original and the resulting positions of the piece of glass. While we were experimenting, we found that we could make a steady-wind-speed environment by using motors, sticks, and the theorem ”v=rω”. The research showed that the angle created by the original and the resulting positions of the piece of glass and wind speed are in direct proportion in low wind velocity in small θ range. Consequently, we\r can derive the formula ” v≒aθ+b” , for each piece of glass (a,b) can be different. In addition, when in bigger θ range, we can use quadratic approximate equation and interpolation to describe the corresponding relation between the angle and wind speed. By adjusting the thickness, mass , and the shape of the glass, we can make anemometers corresponding to different wind velocity ranges.