咦?!田螺長毛了!探討石田螺及其螺殼上附生藻類與環境因子之關係
This research is about two ponds in the Behu park’s and the Dahu park’s snail(Square Mystery Snail：Sinotaia quadrata) in Taipei city of Nei-hu District for research object, carry out the study of the following research proceed: 1.Discriminate the algae species that are growth on the snail shell and which is a kind of interaction with the snail; 2.The influence of the snail and algae with difference of temperature, salinity, pH value and dark ; 3. The factors affect algae growth on snail shell; 4.Use the variation of snail and algae to be a biological incator. The result manifestation: the algae that are growth on snail shell have two kinds, one is Oscillatoriaceae and the other is Cladophora sp. The interaction between algae and snail belong to communalism, but under the condition of lacking of food, the snail then will eat the Cladophora sp. which grow on the shell of other snails. The temperature adapts aspect, upper limit of the feat existence of the snail should be low in 28℃. When over than 28℃, Cladophora sp. as the most strong, Oscillatoriaceae is secondly, and the snail then is most poor. For the maximum tolerance of the salinity, the snail is about 4.38?, Oscillatoriaceae is about 5.00?, Cladophora sp. is then about 5.83?; Under the different salinity for the tolerance , the Cladophora sp. still the most strong, Oscillatoriaceae is secondly, and the snail then is most poor. Under the dark environment, the speed of Oscillatoriaceae begin to be bleaching is very fast than the Cladophora sp.. In the tolerance of pH value range: The snail is about pH=5～10, Oscillatoriaceae is about pH=7～8, Cladophora sp. is about pH=6～8; When the pH value range is in the pH=5～8, the speed of the Oscillatoriaceae occur changing is very fast than Cladophora sp.. The algae are growing on snail shell very different between two ponds, the main reason is water pH value dissimilarly: When pH value over than 8.5, there is no Cladophora sp. to grow on the snail shell, after the pH value to decrease, Oscillatoriaceae then will compare early than Cladophora sp. to grow on the snail shell. Calculate by the classification of the freshwater biological incator : Two organic pollution degree of the ponds may be lain in theβ-mesosaprobic to theα-mesosaprobic, and the polluting degree of the Dahu pond is more seriously. As for two ponds, have already faced what level of eutrophication? Belong to actually which stage of pollution grade? Not only added the classification data of floating and fixative algea in two ponds, and also according to the parts of chemistry analysis method measure of the data makes the substantial evidence, then could carry out the more accurate and thorough study in the days to come steadily studying process.本研究是以臺北市內湖區兩個綠地公園(碧湖公園與大湖公園)池塘內的石田螺(Sinotaia quadrata)為研究對象，進行以下研究目的之探討：1.鑑別石田螺螺殼上藻類的種類及其與石田螺的互動關係；2.溫度、鹽度、酸鹼值及黑暗等環境因子的差異，對石田螺及螺殼上附生藻類的影響；3.影響藻類附生於石田螺螺殼上的因素；4.將石田螺及螺殼上附生藻類的變化作為監測環境因子或水質變異的指標現象。結果顯示：附生於石田螺螺殼上的藻類有顫藻(Oscillatoriaceae)與剛毛藻(Cladophora sp.)兩類；與石田螺的互動關係應屬於片利共生(communalism)，但在缺乏食物的情況下，石田螺則會採食同伴殼上的剛毛藻。溫度適應方面，石田螺適宜生存的溫度上限應低於28℃，超過28℃水溫環境的耐受程度，是以剛毛藻為最強，其次是顫藻，而石田螺則為最差。對於環境鹽度最大耐受度方面：石田螺約為4.38?，顫藻約為5.00?，剛毛藻則約為5.83?；在不同鹽度環境下，鹽度的耐受程度，仍以剛毛藻為最強，其次是顫藻，而石田螺則是最差。在黑暗環境下，顫藻褪色產生白化現象的速度明顯地比剛毛藻要快了許多。在環境酸鹼值耐受的範圍方面：石田螺約在pH＝5～10 之間，顫藻約在pH＝7～8 之間，剛毛藻則約在pH＝6～8之間；而酸鹼值範圍在ｐＨ＝5～8 時，顫藻產生變化的速度明顯地比剛毛藻還要快。而兩樣區池塘水體酸鹼值的不同，應是造成石田螺螺殼藻類附生現象差異的主要原因：當酸鹼值超過8.5 時，螺殼上就無剛毛藻附生，當酸鹼值降下後，顫藻則會比剛毛藻早出現在螺殼上。藉由淡水生物指標的分類推測：兩樣區池塘水體有機污染程度，可能介於β-中腐水性(β-mesosaprobic,βm)至α-中腐水性(α-mesosaprobic,αm)的範圍之間，而Ｄ池塘受污染的程度應會比Ｂ池塘還要更嚴重些。至於兩樣區池塘水體，已面臨了何種優養化的程度？究竟是屬於哪一個階段的污染等級呢？除須補充水體中浮游性及附著性藻類分類的詳細觀察資料外，仍必須參照部分水質化學分析法所測得的數據作佐證，才能在日後持續地研究過程中進行更精確及深入的探討。
Detection Device for Alcoholic Drunk Persons
The purpose of this project was to create a detection device for alcoholic drunk condition in human by using the principle of vapor pressure difference between breath samplings from normal and alcoholic-drunk men. The work comprises of three major steps. The first task was an experiment to determine an average air volume that can be fully blown out from the lungs of non-drunk people as a control. Twenty adult Thai volunteers weighing between 50 - 80 kg (average 59 kg) were used. The average blown out air volume was found to be 369.9 mL, with the range in values from 340-400 mL (sample size N = 20, SD = 15.47). The second task was an experiment to measure relationship between the blown air volume (100-700 mL, both from alcoholic-drunk and control groups of people) and the resulted vapor pressure by using manometer. The vapor pressure of normal breath increased from 400 to 1,600 newton/m2 with increasing blown air volume, whereas that of the alcoholic-drunk was found to be 600 to 1,800 newton/m2. The last task was to create a detection device prototype to gauge the alcoholic content in the human body from the breath. Air volume of 300 mL was arbitrary chosen to trigger lighting up of indicator lamps. The breath samples of low vapor pressure (low amount of alcohol, 21.12-44.00 mL, equivalent to 14.00-29.17 mg%) would trigger a green lamp to light up. A moderate vapor pressure range (medium amount of alcohol, 88-132 mL, equivalent to 58.33-87.57 mg%) would trigger green and yellow lamps to light up while a high vapor pressure (high amount of alcohol, 250 mL, equivalent to 165.72 mg%) would trigger green, yellow and red lamps to all light up. None of the three lamps would light up from (non-alcoholic) breath of control people. This device has also been tested to external group of volunteers. The work in this project has successfully demonstrated a useful application of simple principle in chemistry on partial vapor pressure.
光觸媒(TiO2)對還原重金屬離子之研究
光催化氧化反應以半導體金屬氧化物為催化劑，進行有機性空氣污染物之快速分解反應。其原理係將半導體材質（如：二氧化鈦，TiO2），在適合之光能量照射下，將半導體激發成為具有氧化/還原能力之催化劑，可加速氧化還原反應之進行，迅速分解有機污染物。 研究動機在於利用TiO2在紫外光的照射下，將H2O 分解產生自由基，使其和水中的重金屬離子進行氧化還原反應，期待可以還原水中的重金屬藉以降低水中重金屬離子濃度，同時藉由使用界面活性劑對奈米微粒具保護作用，可回收重金屬奈米微粒。 由實驗結果得知在紫外光照射下，TiO2 使用量0.5 克，AgNO3(aq) 0.01M，照光24 小時其電導度值上升最多且在溶液表面觀察到銀白色銀金屬薄膜生成而所測得銀金屬析出量明顯增加。探討超音波振盪對TiO2還原力的影響得知，超音波震盪的時間越久，所上升的電導度值愈多。 探討常見的界面活性劑（陽離子型及陰離子型）對TiO2還原力的影響：從數據中可觀察到，加入陰離子界面劑時，電導度值明顯上升；而加入陽離子界面活性劑後，電導度值迅速下降，照光後電導度值也不理想。 探討日光及不同頻率的紫外光照射光源對TiO2還原力的影響：發現紫外光的波長愈短，銀金屬析出的量愈多。 探討除氧處理之溶液對TiO2 還原力的影響得知，除氧處理後所配製的AgNO3（aq）0.01M， 經照光24 小時後電導度值明顯上升，且在溶液表面觀察到大片銀白色銀金屬薄膜生成而所測得銀的析出量也大幅增加。 ;In the experiment, we used the properties of T i O 2 that can catalyzed by UV rays and breaking the molecules of water and produce free radicals, which free radicals can redox metallic oxide as accelerator to analyze organic pollutant briskly. We use different shinning time and to find the best effect of the redox reaction . So we want to use this attribute to begin the redox reaction with the metal ion (for example: Ag＋) in water, expecting to reduce them. And then we can use this method to recycle the metal and to reduce the pollution in rivers. Throughout shining UV rays in 24 hours, we can find out the best effect of TiO2 reducing the metal ion solution. We can also find that we use ultrasonic first; the more redox it will have. In this research ,we can observed that if we put more anion surface-active agent, the more redox it will have. We find the effect of UV rays is better than visible light. The most important is that we deoxidize the metal ion solution, we can get the best effect of the redox reaction . In our research，we can't get the satisfing result of the copper sulphate (CuSO4) by TiO2 accelerating the redox reaction under UV rays.
人類粒腺體蘋果酸.活性中心輔.NAD(P) + 結合位置之探討
人類粒線體蘋果酸.可利用NAD+或NADP+為輔.，幫助腫瘤細胞獲得能量，但一般生理條件較傾向以NAD+為輔.。本研究將K346 修改成偏好NADP+之粒線體蘋果酸.家族中具有高度保留性的絲胺酸、及不具極性之丙胺酸，探討為何此酵素較偏好以NAD+為輔.。天然及突變株酵素的催化常數 (kcat)、基質Km 值、及抑制常數 (Ki) 測定結果顯示K346 之點突變不會影響基質Km 值，但K346S 之kcat明顯上升，繼而改變此酵素對NADP+之選擇性。本研究對於人類粒線體蘋果酸.催化機制的了解，有助設計具專一性的活性抑制劑，未來可應用於抑制腫瘤細胞能量來源，進而抑制腫瘤細胞生長。;Human mitochondrial NAD(P)+-dependent malic enzyme can help tumor cells gain energy, using either NAD+ or NADP+ as the cofactor, but prefers NAD+ as the coenzyme. By mutating the K346 to Ser, conserved in NADP+-dependent ME and to Ala with non-polar, we explore why human mitochondrial NAD(P)+-dependent malic enzyme prefers NAD+ as the coenzyme. We measured the proteins kcat, Km and the Ki values. The experiments showed that mutantions don’t affect the Km values, but K346S increased in the kcatvalue, transferring the coenzyme specificity to NADP+. If we develop deeper understanding of the human mitochondrial NAD(P)+-dependent malic enzyme, we can design a specific drag to inactivate the enzyme activity, and inhibit tumor cell growth.
Equtatetor-新一代智慧型數學處理器
此研究的目的是要設計出一套完整編輯顯現數學式、加以計算，並求出解的一套方法與成品。而這項工作的執行者，在此稱之Equatetor 。一般的數學式子，若要計算的話，普通的計算機是不足夠的。原因是它們沒有辦法表現出數學式的「原貌」，例如分號、指數、函數、根號等數學符號混在一起時的情況。於是，我便擬定了一個研究，希望設計出一套更方便且實用的方法。換句話說，我要設計出一個功能強大的工程計算機程式。其中，自然牽扯到數學式子的顯現方式（以MathML 實現），以及計算機科學的演算法及資料結構。我主要的目的有四：(1) 顯示數學式(2) 方便編輯數學式(3) 計算數學式(4) 處理可以以不同形式輸出解答的計算（如輸出分數、根號、函數解等）。研究結果中，成功地運用XML 中的MathML 與二分逼近分數等演算法及若干資料結構，達到了以下實用的幾點：(1) 結構化的數學式編輯(2) 完整地顯示數學式(3) 正確運算並輸出運算式的答案(4) 提供一般數學形式之解（非小數之解）;The object of this study is to design a method and processor which is able to edit, display a mathematical expression representing a number, calculate and output the answer. The executor of this task is called Equatetor. Normal calculators are not adequate for this kind of task. The main reason is that they can’t reveal the original expression, such as fractions, radicals, exponents or mathematic functions. Therefore, a simple and convenient method is needed. To perform the possible way of handling those tasks, a computer program has been written. Several techniques were used, such as MathML, computing algorithms, data structures, and so on. Following are main purposes: (1) Displaying mathematical expressions. (2) Editing mathematical expressions simply. (3) Calculating mathematical expressions. (4) Outputting the answers(in different expressions). And the achievements:(1) Structured methods of editing of mathematical expressions. (2) Displaying mathematical expressions completely. (3) Calculating mathematical expressions precisely. (4) Offering answers in different expressions.
湖光山色下的哀愁--由漂浮的琵琶鼠魚探討外來入侵種與放生行為
本研究自2005 年12 月開始，以臺北市內湖區大湖自公園死亡的漂浮琵琶鼠魚(Many-rayed Sailfin Sucker Catfish )為主要研究對象，探討外來物種與放生行為對大湖生態系的影響。 研究期間共觀察到死亡的琵琶鼠魚隻數計有1085 隻，可記錄到個體形質資料的隻數為910 隻，背鰭棘數則有11 棘、12 棘、13 棘與14 棘四種，分析四種不同棘數的琵琶鼠魚後發現：在體長、頭寬及吻到背鰭長度均無差異(p>0.05)，因此判斷所記錄的個體應是棘甲鯰科(Loricariidae) Pterygoplichthys 屬中的同一種魚種。由檢視魚體並分析記錄數量與環境因子相關性後推測：琵琶鼠魚死亡主因是劇烈起伏的溫度差異，其次是人為因素的刻意傷害。 所記錄到大湖池塘水棲動物有：環節動物、軟體動物、節肢動物、魚類、兩棲動物及爬行動物等六大類共34 種，其中外來物種有16 種，本地入侵物種有3 種，而這些外來動物進入大湖的主要管道應是由個人的棄養或放生行為所造成。琵琶鼠魚因繁殖能力強、具攻擊性、吞食其他魚種卵塊、可適應高污染水體及垂釣客對魚種的篩選等因素，而成為最具生存競爭能力的優勢魚種。不但造成大湖池塘呈現嚴重魚種單一化，更可由靜止水域大量擴散至流動水域。未來如何將政府相關部門、學術研究單位與民間社團等力量結合，共同防範大湖琵琶鼠魚的持續蔓延、宣導民眾正確的放生觀念、積極改善大湖水體污染狀況，並訂定完整之外來物種移除計畫，以期恢復大湖池塘物種歧異度，都將是刻不容緩的重要生態課題。;This research began in December of 2005. Focusing on dead floating Many-rayed Sailfin Sucker Catfish in Dahu Park, Nei-hu Distrct, Taipei city. We discuss the impacts of Alien and of behaviors of the release of captured animals on Dahu Park’s ecosystem. During observation period, there were totally 1085 dead Many-rayed Sailfin Sucker Catfish, of which 910 bodies were found. The number of the thorn bushes on dorsal fins includes 11, 12, 13,and 14. After analyzing four kinds of different thorn bushes, we find that no differences exist in Total length, Head width, Predorsal length (p>0.05). We hence conclude that the recorded object should be species with identical with Loricariidae Pterygoplichthys. With the inspection of the fish’s body and the analysis of the relevance of the recorded quantity and the environmental factor, we infer that the main reason of the Many-rayed Sailfin Sucker Catfish’s death is violent temperature differences and the second is attributed to human’s intentional abuse. The aquatic animals of Dahu Park amount to 34 kinds and six classes such as Annelida, Mollusca, Arthropoda, Pisces, Amphobian, and Reptilia. Among them, 16 kinds that are Alien and 3 kinds are Native Invasive Species. Intentional abandon and release behavior channel of these Alien to Dahu Park. Many-rayed Sailfin Sucker Catfish are highly productive, aggressive, and adaptable to highly polluted water. Besides, they eat ovums from other fishes. Moreover, they are not the fisher’s preference and hence often thrown back into pond once hooked . Thus, they become the most competitive survival fish species in Dahu Park. The high competitive ability of Many-rayed Sailfin Sucker Catfish causes Dahu Park to present a serious unification of fish species. This serious unification of fish species could also be spread from static water areas of Dahu Park to flowing water areas. According to our research, certain urgent ecological issues in Dahu Park are to take precautions against the spread of Many-rayed Sailfin Sucker Catfish, to promote the correct idea of releasing captured animals, to improve Dahu Park’s water pollution, and to stipulate a complete plan about eliminating Alien. Our research suggest that government’s relevant departments, academic research units, and folk corporations should be cooperated to achieve the above four goals. Once the four goals are achieved, we believe that the fish species of Dahu Park will be full of varieties again.
阿拉伯芥AtYAK1 基因5'UTR 中的開放讀序框(uORFs)對基因表現調控之探討
在模式植物──阿拉伯芥（Arabidopsis thaliana）中，AtYAK1（Arabidopsis thaliana Yak1-related protein kinase）是目前發現唯一屬於DYRK（Dual specificity Yak1-Related protein Kinase）的蛋白激?。雖然之前研究已證明，不同物種之DYRKs 和細胞的生長與發育過程有關。然而，其在植物中的生理功能卻尚未被明確地研究報導過。在先前的研究中，為瞭解AtYAK1 在阿拉伯芥內作用之位置，前人選取AtYAK1 基因ATG 上游約2.5 kb 的序列（Upstream Element, 2.5KUSE）建構至一含有GUS（β-glucuronidase）報告基因的質體中，並轉形至阿拉伯芥，進行GUS 組織染色分析。但在初步結果中，並沒有在轉殖株觀察到明顯的GUS 表現。進一步分析，我們發現在2.5KUSE 序列末端約0.5 kb 的5’非轉譯區（5’untranslated region, 5’UTR）中，有四組開放讀序框（Upstream Open Reading Frame, uORF）。有趣的是，許多研究也顯示，uORFs 會影響轉譯過程中的再起始（re-initiation）作用而調控該基因的表現。另一方面，前人亦透過構築好的2KUSE 轉殖株（即不含有5’UTR）進行上述GUS 實驗。結果發現，此2KUSE 轉殖株的GUS 表現非常顯著。本實驗即要瞭解AtYAK1 的uORFs 是否也會影響其蛋白質的合成。首先，我們以點突變的方式將四組uORFs 中之ATG 換成TTG，目的為構築不含有uORFs 之5’UTR（mutated uORFs, ΔuORFs）。在進行原生質體短暫表現分析法（protoplast transient assay）及GUS 組織染色分析後，將結果與含有uORFs 的結果作比較：當缺乏uORFs 後，其3’端報告基因的表現量確實比原來顯著。綜合以上，我們認為此uORFs 對於AtYAK1 蛋白質之表現佔有相當重要的影響地位。最後，我們對5’非轉譯區是否存在開放讀序框進行阿拉伯芥全基因組分析，相關結果亦於本研究報告中分析討論。AtYAK1（Arabidopsis thaliana Yak1-related protein kinase）is the first DYRK（Dual specificity Yak1-Related protein Kinase ） family member identified in the model plant ─ Arabidopsis thaliana and exists as one copy gene in Arabidopsis. Previous studies showed that many eukaryotic DYRKs are involved in regulating the growth and development of cells. However, the study of AtYAK1 in Arabidopsis is lacking to date. In order to understand where AtYAK1 expresses and functions in plants, a 2.5 kb fragment which is located upstream from the major ATG of AtYAK1（termed Upstream Element, 2.5KUSE）was previously constructed to drive the expression of a reporter gene, GUS（β-glucuronidase）, in transgenic Arabidopsis. Much to our surprise, no GUS expression signal could be detected in such transgenic plants. When further analyses were performed, we found that there are four upstream open reading frames （uORFs） in the 5’untranslated region ( 5’UTR ) within the 2.5KUSE. Many studies indicating that the uORFs can regulate the translation of downstream ORF encoding the major gene product through the procedure of translation re-initiation. This action represents a mode of translational regulation for gene expression. Indeed, GUS activity could be readily detected in transgenic plants expression 2KUSE::GUS, a construct lacking the 5’UTR of AtYAK1. In this study, I have tried to elucidate whether the uORFs of AtYAK1 can regulate the translation of the downstream major ORF. First, in order to construct a 5’UTR fragment of which uORFs have been mutated（ΔuORFs）, we apply site-directed mutagenesis to substitute ATG with TTG for the four uORFs and examine the expression of GUS driven by this mutated 2.5KUSE. After analyzing the results in both Arabidopsis protoplast transient assay and transgenic Arabidopsis, stronger expression of reporter genes in both systems were observed when the four uORFs were mutated. We have also confirmed that, in transient expression system, the increase of reporter gene activity was not due to the excess accumulation of the corresponding mRNAs. Rather, it is the four uORFs which play an important role in negatively regulating the translation of AtYAK1, possibly via inhibiting the translation re-initiation of major ORF. A genome-wide examination of uORFs in all Arabidopsis genes was also performed to assess the possible contribution of uORF in regulating gene expression.
E-SBR Production
The objective of this project is to investigate styrene butadiene rubber(SBR), which is the most important synthetic rubber. The project contains production methods, properties, safety methods and applications for emulsion SBR. The goal is to determine which production technique of SBR is the most beneficial. This is based on the chemical and physical properties of the final product and its quality. The process that is used in the project is emulsion SBR with combined coagulation stripping. The rubber production plant is a profitable venture. The total capital cost is estimated around $31,361,572, with an annual production cost of about $67,281,378 yearly, generating revenues of $73,801,760 per fiscal year, with an interest rate of 5%. The life of the plant is 15 years and would produce 50,000 ton/year.