光觸媒(TiO2)對還原重金屬離子之研究
光催化氧化反應以半導體金屬氧化物為催化劑，進行有機性空氣污染物之快速分解反應。其原理係將半導體材質（如：二氧化鈦，TiO2），在適合之光能量照射下，將半導體激發成為具有氧化/還原能力之催化劑，可加速氧化還原反應之進行，迅速分解有機污染物。 研究動機在於利用TiO2在紫外光的照射下，將H2O 分解產生自由基，使其和水中的重金屬離子進行氧化還原反應，期待可以還原水中的重金屬藉以降低水中重金屬離子濃度，同時藉由使用界面活性劑對奈米微粒具保護作用，可回收重金屬奈米微粒。 由實驗結果得知在紫外光照射下，TiO2 使用量0.5 克，AgNO3(aq) 0.01M，照光24 小時其電導度值上升最多且在溶液表面觀察到銀白色銀金屬薄膜生成而所測得銀金屬析出量明顯增加。探討超音波振盪對TiO2還原力的影響得知，超音波震盪的時間越久，所上升的電導度值愈多。 探討常見的界面活性劑（陽離子型及陰離子型）對TiO2還原力的影響：從數據中可觀察到，加入陰離子界面劑時，電導度值明顯上升；而加入陽離子界面活性劑後，電導度值迅速下降，照光後電導度值也不理想。 探討日光及不同頻率的紫外光照射光源對TiO2還原力的影響：發現紫外光的波長愈短，銀金屬析出的量愈多。 探討除氧處理之溶液對TiO2 還原力的影響得知，除氧處理後所配製的AgNO3（aq）0.01M， 經照光24 小時後電導度值明顯上升，且在溶液表面觀察到大片銀白色銀金屬薄膜生成而所測得銀的析出量也大幅增加。 ;In the experiment, we used the properties of T i O 2 that can catalyzed by UV rays and breaking the molecules of water and produce free radicals, which free radicals can redox metallic oxide as accelerator to analyze organic pollutant briskly. We use different shinning time and to find the best effect of the redox reaction . So we want to use this attribute to begin the redox reaction with the metal ion (for example: Ag＋) in water, expecting to reduce them. And then we can use this method to recycle the metal and to reduce the pollution in rivers. Throughout shining UV rays in 24 hours, we can find out the best effect of TiO2 reducing the metal ion solution. We can also find that we use ultrasonic first; the more redox it will have. In this research ,we can observed that if we put more anion surface-active agent, the more redox it will have. We find the effect of UV rays is better than visible light. The most important is that we deoxidize the metal ion solution, we can get the best effect of the redox reaction . In our research，we can't get the satisfing result of the copper sulphate (CuSO4) by TiO2 accelerating the redox reaction under UV rays.
Screening, Isolation, and Characterization of Fluorescent Proteins from Nudibranchs
Fluorescent proteins are used to label and tag tumor cells. These are also used in molecular cloning methods, and in monitoring cellular processes. However, the Philippines does not have a local source of fluorescent proteins, and thus imports these expensive chemicals from other countries. Possible sources of fluorescent proteins may come from marine organisms and certain plants. This work presents the screening, isolation, and characterization of fluorescent proteins from a potential local source—nudibranchs. The pigments of nudibranchs function to make their different body parts, as well as the cerata, brilliantly colored for defense and protection as in camouflage and aposematic behavior. The pigmentation in the skin of the nudibranchs was used as the fluorescent protein source. Crude protein extracts from the seven species of nudibranchs collected from Mabini, Batangas, Philippines were run through Reversed- Phase High Performance Liquid Chromatography (HPLC) for screening. Graphs of the absorbance of the seven species showed which species exhibit the most number of aromatic amino acids, the main component of fluorescent proteins. The species with the highest absorbance at 280 nm, which is Chromodoris willani, was run through the HPLC again for recovery and collection of fractions. The fractions were examined through fluorescence microscopy, wherein the samples were filtered at three emission ranges, the first at 450-470nm,the second at 515-545 nm, and the third at 600-650 nm, which corresponds to blue, green, and red, respectively. The proteins which emit at the said ranges were the only component allowed to pass through. The results, which are images of the excited proteins, showed that fluorescent proteins are present in the fractions of Sample 6—Chromodoris willani.
咦?!田螺長毛了!探討石田螺及其螺殼上附生藻類與環境因子之關係
This research is about two ponds in the Behu park’s and the Dahu park’s snail(Square Mystery Snail：Sinotaia quadrata) in Taipei city of Nei-hu District for research object, carry out the study of the following research proceed: 1.Discriminate the algae species that are growth on the snail shell and which is a kind of interaction with the snail; 2.The influence of the snail and algae with difference of temperature, salinity, pH value and dark ; 3. The factors affect algae growth on snail shell; 4.Use the variation of snail and algae to be a biological incator. The result manifestation: the algae that are growth on snail shell have two kinds, one is Oscillatoriaceae and the other is Cladophora sp. The interaction between algae and snail belong to communalism, but under the condition of lacking of food, the snail then will eat the Cladophora sp. which grow on the shell of other snails. The temperature adapts aspect, upper limit of the feat existence of the snail should be low in 28℃. When over than 28℃, Cladophora sp. as the most strong, Oscillatoriaceae is secondly, and the snail then is most poor. For the maximum tolerance of the salinity, the snail is about 4.38?, Oscillatoriaceae is about 5.00?, Cladophora sp. is then about 5.83?; Under the different salinity for the tolerance , the Cladophora sp. still the most strong, Oscillatoriaceae is secondly, and the snail then is most poor. Under the dark environment, the speed of Oscillatoriaceae begin to be bleaching is very fast than the Cladophora sp.. In the tolerance of pH value range: The snail is about pH=5～10, Oscillatoriaceae is about pH=7～8, Cladophora sp. is about pH=6～8; When the pH value range is in the pH=5～8, the speed of the Oscillatoriaceae occur changing is very fast than Cladophora sp.. The algae are growing on snail shell very different between two ponds, the main reason is water pH value dissimilarly: When pH value over than 8.5, there is no Cladophora sp. to grow on the snail shell, after the pH value to decrease, Oscillatoriaceae then will compare early than Cladophora sp. to grow on the snail shell. Calculate by the classification of the freshwater biological incator : Two organic pollution degree of the ponds may be lain in theβ-mesosaprobic to theα-mesosaprobic, and the polluting degree of the Dahu pond is more seriously. As for two ponds, have already faced what level of eutrophication? Belong to actually which stage of pollution grade? Not only added the classification data of floating and fixative algea in two ponds, and also according to the parts of chemistry analysis method measure of the data makes the substantial evidence, then could carry out the more accurate and thorough study in the days to come steadily studying process.本研究是以臺北市內湖區兩個綠地公園(碧湖公園與大湖公園)池塘內的石田螺(Sinotaia quadrata)為研究對象，進行以下研究目的之探討：1.鑑別石田螺螺殼上藻類的種類及其與石田螺的互動關係；2.溫度、鹽度、酸鹼值及黑暗等環境因子的差異，對石田螺及螺殼上附生藻類的影響；3.影響藻類附生於石田螺螺殼上的因素；4.將石田螺及螺殼上附生藻類的變化作為監測環境因子或水質變異的指標現象。結果顯示：附生於石田螺螺殼上的藻類有顫藻(Oscillatoriaceae)與剛毛藻(Cladophora sp.)兩類；與石田螺的互動關係應屬於片利共生(communalism)，但在缺乏食物的情況下，石田螺則會採食同伴殼上的剛毛藻。溫度適應方面，石田螺適宜生存的溫度上限應低於28℃，超過28℃水溫環境的耐受程度，是以剛毛藻為最強，其次是顫藻，而石田螺則為最差。對於環境鹽度最大耐受度方面：石田螺約為4.38?，顫藻約為5.00?，剛毛藻則約為5.83?；在不同鹽度環境下，鹽度的耐受程度，仍以剛毛藻為最強，其次是顫藻，而石田螺則是最差。在黑暗環境下，顫藻褪色產生白化現象的速度明顯地比剛毛藻要快了許多。在環境酸鹼值耐受的範圍方面：石田螺約在pH＝5～10 之間，顫藻約在pH＝7～8 之間，剛毛藻則約在pH＝6～8之間；而酸鹼值範圍在ｐＨ＝5～8 時，顫藻產生變化的速度明顯地比剛毛藻還要快。而兩樣區池塘水體酸鹼值的不同，應是造成石田螺螺殼藻類附生現象差異的主要原因：當酸鹼值超過8.5 時，螺殼上就無剛毛藻附生，當酸鹼值降下後，顫藻則會比剛毛藻早出現在螺殼上。藉由淡水生物指標的分類推測：兩樣區池塘水體有機污染程度，可能介於β-中腐水性(β-mesosaprobic,βm)至α-中腐水性(α-mesosaprobic,αm)的範圍之間，而Ｄ池塘受污染的程度應會比Ｂ池塘還要更嚴重些。至於兩樣區池塘水體，已面臨了何種優養化的程度？究竟是屬於哪一個階段的污染等級呢？除須補充水體中浮游性及附著性藻類分類的詳細觀察資料外，仍必須參照部分水質化學分析法所測得的數據作佐證，才能在日後持續地研究過程中進行更精確及深入的探討。
Equtatetor-新一代智慧型數學處理器
此研究的目的是要設計出一套完整編輯顯現數學式、加以計算，並求出解的一套方法與成品。而這項工作的執行者，在此稱之Equatetor 。一般的數學式子，若要計算的話，普通的計算機是不足夠的。原因是它們沒有辦法表現出數學式的「原貌」，例如分號、指數、函數、根號等數學符號混在一起時的情況。於是，我便擬定了一個研究，希望設計出一套更方便且實用的方法。換句話說，我要設計出一個功能強大的工程計算機程式。其中，自然牽扯到數學式子的顯現方式（以MathML 實現），以及計算機科學的演算法及資料結構。我主要的目的有四：(1) 顯示數學式(2) 方便編輯數學式(3) 計算數學式(4) 處理可以以不同形式輸出解答的計算（如輸出分數、根號、函數解等）。研究結果中，成功地運用XML 中的MathML 與二分逼近分數等演算法及若干資料結構，達到了以下實用的幾點：(1) 結構化的數學式編輯(2) 完整地顯示數學式(3) 正確運算並輸出運算式的答案(4) 提供一般數學形式之解（非小數之解）;The object of this study is to design a method and processor which is able to edit, display a mathematical expression representing a number, calculate and output the answer. The executor of this task is called Equatetor. Normal calculators are not adequate for this kind of task. The main reason is that they can’t reveal the original expression, such as fractions, radicals, exponents or mathematic functions. Therefore, a simple and convenient method is needed. To perform the possible way of handling those tasks, a computer program has been written. Several techniques were used, such as MathML, computing algorithms, data structures, and so on. Following are main purposes: (1) Displaying mathematical expressions. (2) Editing mathematical expressions simply. (3) Calculating mathematical expressions. (4) Outputting the answers(in different expressions). And the achievements:(1) Structured methods of editing of mathematical expressions. (2) Displaying mathematical expressions completely. (3) Calculating mathematical expressions precisely. (4) Offering answers in different expressions.
阿拉伯芥AtYAK1 基因5'UTR 中的開放讀序框(uORFs)對基因表現調控之探討
在模式植物──阿拉伯芥（Arabidopsis thaliana）中，AtYAK1（Arabidopsis thaliana Yak1-related protein kinase）是目前發現唯一屬於DYRK（Dual specificity Yak1-Related protein Kinase）的蛋白激?。雖然之前研究已證明，不同物種之DYRKs 和細胞的生長與發育過程有關。然而，其在植物中的生理功能卻尚未被明確地研究報導過。在先前的研究中，為瞭解AtYAK1 在阿拉伯芥內作用之位置，前人選取AtYAK1 基因ATG 上游約2.5 kb 的序列（Upstream Element, 2.5KUSE）建構至一含有GUS（β-glucuronidase）報告基因的質體中，並轉形至阿拉伯芥，進行GUS 組織染色分析。但在初步結果中，並沒有在轉殖株觀察到明顯的GUS 表現。進一步分析，我們發現在2.5KUSE 序列末端約0.5 kb 的5’非轉譯區（5’untranslated region, 5’UTR）中，有四組開放讀序框（Upstream Open Reading Frame, uORF）。有趣的是，許多研究也顯示，uORFs 會影響轉譯過程中的再起始（re-initiation）作用而調控該基因的表現。另一方面，前人亦透過構築好的2KUSE 轉殖株（即不含有5’UTR）進行上述GUS 實驗。結果發現，此2KUSE 轉殖株的GUS 表現非常顯著。本實驗即要瞭解AtYAK1 的uORFs 是否也會影響其蛋白質的合成。首先，我們以點突變的方式將四組uORFs 中之ATG 換成TTG，目的為構築不含有uORFs 之5’UTR（mutated uORFs, ΔuORFs）。在進行原生質體短暫表現分析法（protoplast transient assay）及GUS 組織染色分析後，將結果與含有uORFs 的結果作比較：當缺乏uORFs 後，其3’端報告基因的表現量確實比原來顯著。綜合以上，我們認為此uORFs 對於AtYAK1 蛋白質之表現佔有相當重要的影響地位。最後，我們對5’非轉譯區是否存在開放讀序框進行阿拉伯芥全基因組分析，相關結果亦於本研究報告中分析討論。AtYAK1（Arabidopsis thaliana Yak1-related protein kinase）is the first DYRK（Dual specificity Yak1-Related protein Kinase ） family member identified in the model plant ─ Arabidopsis thaliana and exists as one copy gene in Arabidopsis. Previous studies showed that many eukaryotic DYRKs are involved in regulating the growth and development of cells. However, the study of AtYAK1 in Arabidopsis is lacking to date. In order to understand where AtYAK1 expresses and functions in plants, a 2.5 kb fragment which is located upstream from the major ATG of AtYAK1（termed Upstream Element, 2.5KUSE）was previously constructed to drive the expression of a reporter gene, GUS（β-glucuronidase）, in transgenic Arabidopsis. Much to our surprise, no GUS expression signal could be detected in such transgenic plants. When further analyses were performed, we found that there are four upstream open reading frames （uORFs） in the 5’untranslated region ( 5’UTR ) within the 2.5KUSE. Many studies indicating that the uORFs can regulate the translation of downstream ORF encoding the major gene product through the procedure of translation re-initiation. This action represents a mode of translational regulation for gene expression. Indeed, GUS activity could be readily detected in transgenic plants expression 2KUSE::GUS, a construct lacking the 5’UTR of AtYAK1. In this study, I have tried to elucidate whether the uORFs of AtYAK1 can regulate the translation of the downstream major ORF. First, in order to construct a 5’UTR fragment of which uORFs have been mutated（ΔuORFs）, we apply site-directed mutagenesis to substitute ATG with TTG for the four uORFs and examine the expression of GUS driven by this mutated 2.5KUSE. After analyzing the results in both Arabidopsis protoplast transient assay and transgenic Arabidopsis, stronger expression of reporter genes in both systems were observed when the four uORFs were mutated. We have also confirmed that, in transient expression system, the increase of reporter gene activity was not due to the excess accumulation of the corresponding mRNAs. Rather, it is the four uORFs which play an important role in negatively regulating the translation of AtYAK1, possibly via inhibiting the translation re-initiation of major ORF. A genome-wide examination of uORFs in all Arabidopsis genes was also performed to assess the possible contribution of uORF in regulating gene expression.
口蹄疫病毒鞘蛋白rVP1 誘發Prohibitin 之遷移
細胞凋亡具有控制生物體細胞數目之功能，能讓特定的細胞走向死亡，因此若能掌握其作用機制，便可能藉由調控細胞凋亡的發生，進而應用於癌症治療。前人研究（2.）指出，經基因重組技術純化之口蹄疫病毒鞘蛋白rVP1，會導致BHK-21的Akt 蛋白質去活化，引起細胞凋亡的現象。然而在其後續的研究中，卻發現到在BHK-21中大量表現磷酸化的Akt 蛋白質，並無法反轉由rVP1 所誘發之細胞凋亡。因此本實驗利用二維蛋白質電泳，尋找Akt pathway 以外之細胞凋亡相關蛋白質。目前已證明Prohibitin 此一蛋白質，在由rVP1 所引起之細胞凋亡中，有自細胞核移動至細胞質的現象。此外，亦經由實驗排除Prohibitin 位於Akt pathway 的可能性。Western Blot 之結果更顯示，經rVP1 處理後，Prohibitin 在很短的時間內便出現遷移的現象，故推測其具有調控細胞凋亡上游反應的功能。Apoptosis can lead some specific cells to programmed death, thus, it is a major way for creatures to control their cells amounts. If we can command the mechanism of apoptosis, we may use it as a therapy for cancer by artificial regulation of apoptosis. VP1 is one of the capsid proteins of Foot and Mouth Disease Virus (FMDV). A research (2.) has indicated that the recombinant VP1 (rVP1) can result in dephosphorylation of Akt in BHK-21, and then lead the cells to apoptosis. However, in their follow-up experiments, they discovered that even if they expressed great amount of phospho-Akt in BHK-21, it still couldn’t reverse the apoptosis induced by rVP1. Therefore, this experiment takes the advantage of two-dimension protein electrophoresis (2D) in order to find apoptotic proteins excluded from the Akt pathway. I have found that Prohibitin exports from nucleus to cytosol after rVP1 treatment. Furthermore, I eliminate the possibility that Prohibitin’s may be located in Akt pathway. The results of Western Blot also shows that protein amount of Prohibitin in BHK-21 increase after rVP1 treatment, hence the purpose of nuclear export of Prohibitin might not be to degrade it. It might have some much more important function in the process of exportation. Besides, Prohibitin exports to cytosol in quite a short time after rVP1 treatment. According to this phenomenon, I suppose that Prohibitin has a role as a regulator of apoptotic up-stream reactions.
利用Google Maps 建立高中地理資訊系統
台灣通史「婆娑之洋，美麗之島」，身為台灣人，應知台灣事，本研究本維 基百科（Wikipedia）所揭示的「海納百川，有容乃大」精神，以Apache 網頁伺 服器為平台，MySQL 為資料庫，利用AJAX、PHP 網頁技術，並使用Google Maps 提供之服務，建立符合Web 2.0 精神的的b04地理資訊共享系統。在此系統下， 我們允許教師(編輯者)以合作方式上網提供與課程相關的地理資訊，並可進行資 訊修改或刪除，希望集合所有教師之力，提供給學生最豐富、最正確的b04地理 資訊系統。 In this paper, we propose a new map-based collaboration environment “MyGIS” for geographical information distribution. In MyGIS, the publishers/teachers can easily collaborate to annotate a spot with contents such as messages and links. The annotations of a spot are updated on the map in real-time which enables the receivers/students to correctly study at anywhere and anytime. To ensure the trustfulness of the content, the publishers are authenticated using login mechanism. In this study, several technologies inc lu ding Apache, PHP, MySQL, Ajax are involved. Finally, We implemented MyGIS on Google Maps and evaluated its feasibility and usefulness.
黑擬蛺蝶(Junonia iphita iphita)的幼蟲生存策略
黑擬蛺蝶(Junonia iphita iphita)為台灣地區常見的蛺蝶，其幼蟲具有築巢行為，但文獻中對其幼蟲行為的描述極少，因此本實驗探討黑擬蛺蝶幼蟲在野外的族群變化與環境之關係，並研究幼蟲築巢行為，以探討其適應環境的生存策略。首先於室內及恆溫生長箱中飼養幼蟲，以建立其生活史基本資料，並於野外統計各齡期幼蟲在不同植物上的數量變化及築巢行為的差異，以探討不同環境因素對幼蟲築巢之影響。由實驗結果得知，黑擬蛺蝶生活史短，可取食多種爵床科(Acanthaceae)之植物，其寄主植物除文獻所紀錄之台灣馬藍(Strobilanthes formosanus)及賽山藍(Blechum pyramudatum)等外，也取食大安水蓑衣(Hydrophila pogonocalyx)、無花水蓑衣(Hygrophila violacea)、易生木(Hemigraphis repanda)及翠蘆利(Ruellia brittoniana)等。黑擬蛺蝶幼蟲野外族群波動與溫溼度及雨量等環境因子並無直接關係。黑擬蛺蝶一年發生多世代且有世代重疊情形，世代波動與四季律動關係不明顯，顯示黑擬蛺蝶對環境的適應力大。黑擬蛺蝶幼蟲利用築巢以適應環境變化，應是其幼蟲良好的生存策略。且幼蟲在強風及光線強的環境下築巢率增加，降雨時則減少。Junonia iphita iphita belongs to Nymphalidae（Lepidoptera）. They can be found easily in the wilderness of Taiwan. Its larva shows nest-making behavior. However, there is little literature documenting the behaviors of its larva. Therefore, the purpose of this research is to investigate the relationship between the quantities of Junonia iphita iphita’s larvae and its natural habitat, to research its nesting behaviors, and to investigate its survival strategies to adapt to the environment. I started by raising larvae in a growth chamber under a controlled temperature in order to obtain its initial information regarding its life history. In the field, I documented the numbers and the changes of larvae at each stage on different host plants and recorded the differences in its nest-making behavior in order to find out which environment factors influence the nest-making of Junonia iphita iphita’s larvae. These experiments concluded that the life history of larvae is short. The immature intaking habit showed that the larva takes various plants of the Acanthaceae. In addition to the host plants mentioned in the literature, such as Strobilanthes formosanus, and Blechum pyramidatum, Junonia iphita iphita’s larvae also live by Hydrophila pogonocalyx, Hygrophila violacea, Hemigraphis repanda, and Ruelba brittoniana, which were not listed in the literature. Through experiments, I discovered that there is no direct correlation between the population fluctuations of larvae and it’s enviroment including factors such as temperature, humidity or rainfall. Junonia iphita iphita can produce multi-generations in a year accompanying generation overlapping. There is also no obvious correlation between the generation fluctuations and changing seasons, showing that larvae can easily adapt to the environment. Junonia iphita iphita’s larvae adapt themselves to the different environments by nest-making which should be a good survival strategy. Besides, the rate of nest-making increases when larvae are under strong winds and strong lights and decreases when the rain falls.