Investigating Novel Methods to Reduce Cholesterol Levels
An increase in blood cholesterol contributes to cardiovascular diseases, the number one cause of death worldwide. Statins are currently the most effective in reducing cholesterol levels and treating patients with high cholesterol. However, these pharmaceutical agents have been shown to cause several side effects, prompting the need for a more natural solution to increasing cholesterol levels. Hence, a study was conducted to investigate the ability of lactic acid bacteria in the removal of cholesterol, explore the mechanism for the removal of cholesterol by lactic acid bacteria, and examine the effectiveness of kidney beans and sunflower seeds in inhibiting HMG-CoA reductase in the cholesterol biosynthesis pathway. Results showed that Lactobacillus plantarum was the most effective in reducing cholesterol levels and that the mechanism for cholesterol removal included both the binding to cell wall and active uptake into cells. Sunflower seeds and kidney beans were also shown to be effective in inhibiting HMG-CoA reductase, with sunflower seeds having 100% inhibition of the enzyme, similar to pravastatin, a commercial cholesterol reducing drug, and kidney beans having comparable percentage inhibition of the enzyme compared to pravastatin.
Bioinsecticide vs Aedes aegypti, vector of dengue, zika and chikungunya
The purpose of this research is to make an ecological insecticide that mixes the extracts of Piper tuberculatum, Annona muricata and Melia azedarach, that together in application cause mortality and repellence of the mosquito Aedes aegypti with the intention to help in the control of the diseases this mosquito is guilty of: dengue, zika and chikungunya, and decrease the risk of infection by a safe and organic way.
Microbial Film Power Generation 2.0 - It’s about to get cooler
This study demonstrates that microbial film power generation is a potentially viable source of alternative energy. This research occurred over a period of two years. In the first year (2016) I tested a new method of generating renewable energy, referred to as microbial film power generation. I showed that electricity could be captured from microbial decomposition using solid graphite plates (29cm x 20cm) placed in lightly decomposed muskeg (collected in northern British Columbia). In the second year (2017) the purpose was to increase the power output of the fuel cell, while also compacting the setup. Certain changes were made to the experimental set up, namely the use of spongy graphite felt in place of solid graphite plates, thus providing a larger surface area for microbial activity to occur. The new fuel cells made produced about twice as much power. Not only was the power output greater, but it was produced from a much smaller area: 7.82 mWh/cm2 on graphite felt, compared to 0.21 mWh/cm2 on graphite plates. In other words, graphite felt produced 37 times more power per unit area than graphite plates. Furthermore, it would appear that by removing the load from the fuel cell for approximately 24 hours, the fuel cell could essentially recharge. This may be due to microbial activity releasing more electrons onto the anode permitting a new cycle to take place. This would suggest that the system could naturally recharge itself.
Bioplastic - The Future is Degradable Plastics. Investigating Biodegradation of Polyhydroxybutyrate Bioplastic by 紐西蘭 Soil Microorganisms
The rate and production of conventional petroleum based plastics is unsustainable and not eco-friendly. Plastics often end up in marine environments and can take hundreds of years to decompose in landfills. According to Statistica, in 2015 alone, global plastic production was approximately 322 million metric tonnes and is projected to increase in the future. PHB bioplastic or Polyhydroxybutyrate is both biologically produced and biodegradable and can serve as a viable alternative to conventional plastics. But can it be broken down by soil microbes within a reasonable time frame? I have set out to answer this question. My aim was to isolate and analyse microorganisms from the Rotorua area that are capable of degrading Polyhydroxybutyrate (PHB) bioplastic . I isolated PHB degrading microorganisms from Rotorua soils by culturing on an agar based mineral salt media supplemented with PHB powder (MSM PHB agar). Samples were taken from Mount Ngongotaha and Te Puia geothermal soils as well as Okareka, termite frass and termite guts. One isolate from the Te Puia sample (labelled G2) was found to successfully degrade PHB powder. After isolation and purification of the G2 isolate, it was cultured on a range of media types to examine properties exhibited under differing nutrient conditions. Multiple organisms were found to be involved in the degradation of PHB bioplastic and work together symbiotically, this included bacteria and fungi which was identified as penicillium. The sample isolated from Te Puia soils (site 2 – G2Clear) in the Rotorua environment was found capable of competently degrading PHB, clearing 8% of PHB after 26 days. The G2Clear isolate is a mixture of bacteria and fungi working in an endosymbiotic relationship to degrade PHB and are unable to successfully degrade PHB individually. It is through the secretion of an extracellular PHB depolymerase enzyme that PHB is degraded, conforming with my hypothesis. This proves that PHB bioplastic is a viable alternative to conventional petroleum based plastics as PHB can be relatively quickly broken down by soil microorganisms.