長期處理兒茶素對無機砷與氯化鎘之遺傳毒性
重金屬對人體傷害的無遠弗屆-人人皆知，根據流行病學的研究指出，長期處\r 在重金屬含量過高之地區，易造成生理病變。比方說，長期生活在砷含量高的環境\r 中，易導致肝臟、周邊血管及神經系的損害，各種癌症的發生機率也大為提高；長\r 期處在含量過高的鎘環境中，易引起不正常的鈣代謝﹐產生骨質疏鬆症、軟骨症、\r 糖尿、胺基尿酸、尿蛋白、貧血、和肝功能異常的症狀；而目前坊間所看到各類茶\r 品中含有的兒茶素則被發現具有抗氧化、抗突變及清除自由基等功能。故本實驗以\r 人類胃癌細胞(SC-M1)為材料，以微核(micronuclei)偵測技術觀察細胞長期處理兒茶\r 素((-)-epigallocatechin-3-gallate, EGCG)後，對於亞砷酸鈉(sodium arsenite, NaAsO?)\r 及氯化鎘(cadmium chloride, CdCl?)所造成的細胞遺傳毒性有何影響。另一方面則利\r 用SRB 分析法測量細胞存活率，探討長期處理兒茶素後，亞砷酸鈉及氯化鎘對於細\r 胞的毒殺情況是否改變。實驗結果顯示細胞長期處理兒茶素不會造成微核的增加，\r 但是卻會增加細胞的存活率；單獨處理亞砷酸鈉和氯化鎘則都會使細胞中微核明顯\r 的增加，且會降低細胞的存活率；而長期處理兒茶素的細胞對亞砷酸鈉所誘發的微\r 核有抑制作用且會增加細胞對於亞砷酸鈉的耐受度，長期處理兒茶素無法抑制氯化\r 鎘所誘發的微核與細胞毒性。\r \r \r It is well documented that exposure to heavy metals could cause seriously adverse\r effects to humans. Epidemiological evidence has shown that illness is frequently\r observed in residents living long-term in heavy metal contaminated area. For example,\r long-term exposure of arsenic was associated with increased incidences of liver diseases,\r peripheral vascular and neurological diseases, as well as cancers. And long-term exposed\r to cadmium may lead to abnormal metabolism of calcium, osteoporosis, osteomalacia,\r glucouria, aminoaciduria, proteinuria, anemia and abnormal liver function. Recently,\r numerous reports have shown that (-)-epigallocatechin-3-gallate (EGCG), the major\r polyphenol from green tea, have ability to anti-oxidative stress and anti-mutagenesis. In\r this report, we investigated the effects of EGCG long-term exposure in sodium arsenite\r and cadmium chloride induces chromosomal damages and cytotoxicity in a gastric cancer\r cell line, SC-M1. Our present results demonstrated cells long-term exposure to EGCG did\r not induce micronuclei (MN) formation but induce the growth rate. Sodium arsenite or\r cadmium chloride alone significantly induced MN formation and cytotoxicity in cells\r without long-term EGCG exposure. However, long-term treatment of SC-M1 cells with\r EGCG significantly reduced MN formation and protects the cells from cytotoxicity\r induced by arsenite. Long-term exposure of EGCG had no effect on MN induction and\r cell survival in cadmium-treated SC-M1 cells.
正N 邊形光圈之路徑追蹤
本研究是[對於正n 邊形A1A2…An邊上一點P(含頂點)，想像自定點P 朝鄰邊發出一條光線，若依逆(順)時針方向依序與每邊皆碰撞一次，經一圈而可回到P 點，則此路徑稱為「光圈」。過程試著追蹤在正n 邊形內能形成光圈的光線行進路徑及其相關問題。
本研究令，且以逆時針得光圈來討論：
1.根據[光的反射原理]，探討光圈之存在性，發現除定點P 在正2m 邊形或正三角形的頂點外，其餘皆有光圈。
2.將可形成光圈的路徑圖展開成[直線路徑圖]來探討。
3.由[直線路徑圖]，觀察到形成光圈的光線行進路徑，可能存在下列情況： (1)不通過正n 邊形的頂點，且產生路徑循環與不循環問題。 (2)通過正n 邊形的頂點。
4.發現正2m 邊形光圈皆為[完美光圈]。
5.發現正2m+1 邊形光圈之路徑與有理數、無理數之特質有關。即當s 值為有理數時，路徑會循環；當s 值為無理數時，路徑不循環。
The research is about [on Point P (including the angles) on the side of regular polygons A1、A2…An , imagine the light goes from Point P to the closest side, then bumps each side sequentially counterclockwise. After going a circle, it’s back to Point P. The track is called “the circle of light.” I try to trace the light track of the circle of light and other correlative questions.]
In this research, we suppose,and we discuss the circle of light according counterclockwise direction:1.According to the light reflective principles, we discuss whether the circle of light exists or not. And then we discover that the circle of light really exists except when Point P is on the angles of regular triangle or regular 2m polygons. 2.Spread out the circle of light’s track to [rectilinear track.] 3.By [the picture of rectilinear track], observing there are two kinds of the circle of light’s track: (1)If the light doesn’t go through the angles of regular polygons, it can be a circulative track or a non-circulative track. (2)When the light goes through the angles, it stops. 4.We discover that all the circles of light in regular 2m polygons are [the perfect circles of light.] 5.We discover the circle of light’s track is correlative with rational numbers and irrantional numbers. When s is a rational number, the track is circulative, if s is a irrantional number, the track is not circulative.
毛細管內液體流速之探討及黏度測量方法之創新
有一次上實驗課時看到鄰桌的同學正以實驗室的毛細管吸吮一瓶\r 葡萄汁，每當他吞嚥時，毛細管內殘餘的葡萄汁竟然以近似等速的狀\r 態降回果汁瓶裡，這似乎違背了重力加速度的常理，是毛細管的影響\r 嗎？還是葡萄汁特有的現象？這與毛細管插入葡萄汁的深度有關嗎？\r 這一連串的問題皆因升學壓力而無暇深究。\r 升上高中的那年暑假，我參加了學校舉辦的資優科學營，在物理\r 實驗課中恰好遇到了以毛細管測量液體黏度的實驗，使我有機會深入\r 思索毛細管內液體流速的問題。這個實驗很不好做，要調整兩個定高\r 容器，使水平毛細管兩端產生固定的壓差，讓流經管內的液體為等速\r 的流動。大家手忙腳亂地操作了近三小時，測出來的黏度不僅和老師\r 公布的答案差距甚大，而且各組同學所測得之結果也大相逕庭，不過\r 我們都體驗到了液體黏度對流速的影響。\r 實驗的誤差為什麼那麼大？扣除人為操作誤差後儀器本身還有多\r 大改進空間？有否更精簡的方法測量黏度？毛細管中液體的流速真\r 是固定的嗎？把毛細管斜置或是垂直架置，流速還會固定嗎？重力不\r 會影響流速嗎？若流速真為固定的，如何控制或改變流速呢？是否可\r 以利用流速來測出液體黏度？\r 由於我對這個實驗的專注，在學校選修的專題課程中即以此為題\r 目做長期的研究，在老師的指導下，我和同組同學不停地查閱有關書\r 籍及整理資料，漸漸研究清楚原理後定出實驗計畫，並開始購買材料\r 與工具，長期地動手實驗及改進，兩年來我們利用毛細管中液體的等\r 速及不等速流動的特性，發展出了兩種既精簡又準確的液體黏度測量\r 法，以下就是整個研究及創作的過程。\r \r \r The viscosity is one of the most important parameters of the fluids. In conventional viscosity experiments, capillary-based viscometers are widely adopted because of their user-ready devices and moderate prices. However, to accurately measure the viscosity, the fluid velocity must be kept constant; otherwise, the fluctuation may cause the serious deviation. Besides, traditional capillary viscometers often require a long time to make the measurement. This research aims at exploring the capillary fluid mechanics and developing accurate and rapid methods to measure the viscosity. In this study, we developed two creative viscosity-measuring methods, the gravity sloping helical structure and the gradational liquid-level difference, and constructed two cost-effective capillary viscometer prototypes accordingly. The gravity sloping helical structure employs a long helical capillary as the flow channel and utilizes the gravity force to keep the fluid velocity extremely constant. The highlight of this method is that we can see the fluid flow through the capillary directly. On the other hand, the gradational liquid-level difference method uses the pressure sensors to precisely monitor the liquid-level difference, which drives the flow in the capillary, and allows automatic measurement of viscosity. Compared with computer simulation, the experimental results agreed well with the theoretical values. The gravity sloping helical and gradational liquid-level difference methods achieve 0.5% and 0.8% accuracy respectively. In addition, the automation of viscosity sensing also greatly facilitates the viscosity measurement. Furthermore, these two methods are both valuable for incorporating into educational purpose and industrial application.
超聲波在液體的探討
本實驗一開始主要探討超聲波在水中的基本性質，如：指向性、衰減性…等。實驗發現，超聲波的衰減會同時與其指向性以及衰減性有關。 接著希望利用超聲波在水中的物理性質，近一步測量超聲波在水中的聲速，實驗中則利用駐波以及聲光效應測量。在駐波法測量聲速的實驗中，用洗淨機當作聲源，內部放置量筒，量筒內盛水後放入木屑，並使聲波在其中產生駐波即聲浮現象，求出波長後反推聲速，測量出的聲速誤差值僅有1.13%，而在使用聲光效應測量聲速的實驗中，使1.65 MHz 的超聲波在自製的壓克力容器內部所裝的水中產生駐波後，以波長650 nm 的紅光雷射通過，在遠處屏幕即產生似於光柵繞射現象，藉著屏幕上的繞射條紋反推該液體聲速，測量出聲速誤差均在5%以下。 在觀察聲光效應實驗中，發現過段時間後有氣泡產生，由文獻上，得知此現象為超聲空蝕現象(Acoustic cavitation)，就設計實驗測量聲場中聲壓分部，並利用蠟紙觀察氣泡的成長。實驗發現聲場中的聲壓強度以及液體的表面張力和蒸汽壓會影響到產生空蝕的臨界值及產生氣泡的數量。 ;At the beginning this experiment explores the ultrasonic base in the water, including its velocity, the physics property of liquid, direction, and attenuation etc. . . At first, we use methods of standing wave to measure the velocity of sound under the water, using an ultrasonic cleaner as the sound source and putting some wooden powder in the water. As the standing wave accrues/produces, the powder will “stand still.” To measure the length between two grains of powder, in this way we can calculate sound velocity. Another method we use is diffraction of optics. Put 1.65 MHz source and water in a transparent container; then using laser through it. At the board much diffraction light stripes are created. By this way, we can estimate the velocity. Following these ways can calculate velocity precisely. In these experiments, some bubbles create in the container are discovered. We learn it is so-called “Acoustic Cavitation” based on the reference paper. Besides, we design experiment to know the bubbles’ growth and the number of the bubbles is connected to the physics property of liquid. We use different kinds of liquid with different vapor pressure and surface tension. Finally, we know when it has the smaller surface tension and bigger vapor pressure, the liquid makes bubble velocity grow faster and larger amount of bubbles are produced.
金奈米粒子的合成與其光譜特性
當世界各國正全力朝奈米科技發展，而奈米微粒對環境及人類健康影響與否未明朗之際，有關奈米微粒對人類健康影響之探討是刻不容緩之事。本研究的近程目標是合成金奈米粒子，遠程目標是擬探討金奈米粒子的生物毒性。此份研究主要是描述金奈米粒子的最大表面電漿吸收光譜特性及其最大吸收波長，而金奈米粒子的合成是利用四氯金酸( HAuCl4 )與還原劑—硼氫化鈉( NaBH4 )、聯胺水合物( N2H5OH )或檸檬酸鈉在陽離子界面活性劑的溶液( CH 3(CH2)15N+(CH3)3Br– ，溴化十六烷基三甲基銨，CTAB ) 當保護劑中，利用氧化還原方法直接生成金奈米粒子，並探討四氯金酸的濃度、還原劑的濃度、界面活性劑的濃度及反應溫度，對金奈米粒子生成之影響，另外也探討金奈米粒子的穩定性。有關金奈米粒子的光譜特性則利用UV/Vis 光譜儀測試。研究結果發現在相同的條件下，利用冰浴，以NaBH4 當還原劑所合成出的金奈米粒子之平均粒徑為11.70 nm ，而以N2H5OH 當還原劑所合成出的金奈米粒子之平均粒徑為20.25 nm， 有關金奈米粒子之結構及大小藉由穿透式電子顯微鏡( TEM ) 拍攝出的影像得以驗證。;This study mainly focused on the properties of the maximum surface plasmon absorption of gold nanoparticles and their wavelengths. The synthesis of gold nanoparticles made use of HAuCl4 aqueous solution and reducing agent, through the oxidation reduction method, with reductant, sodium borohydride ( NaBH4 ), hydrazine ( N2H5OH ) or sodium citrate solution in the presence of the cetytrimethylammonium bomide ( C19H42BrN,CTAB ) aqueous solution as protecting agent. The influence of concentration of HAuCl4, reductant, surfactant, and temperature on the synthesis of gold nanoparticles were explored. In addition, the stability of the gold nanoparticles was also explored. Measurements of spectral properties of gold nanoparticles were done by UV/Vis spectrometer.When NaBH4 was used as a reductant, the average diameter of gold nanoparticles was 11.70 nm. When N2H5OH was used as a reductant, the average diameter of the synthesized gold nanoparticles was 20.25 nm. The structures and sizes could be identified by the transmission of electron micrograph (TEM) images.