美麗的陷阱 - 探討線蟲捕捉菌之捕蟲機制及應用
線蟲為植物發生病蟲害感染的病源之一，而台灣的松樹，目前正面臨著松材線蟲入侵的危機。從文獻的探討中，發現線蟲有其自然界的天敵 － 線蟲捕捉菌。本實驗著重在探討線蟲捕捉菌特殊的捕捉機制。當線蟲捕捉菌附近出現線蟲時，會展生誘引線蟲的物質，並設計了一步步的實驗，去探討此誘引物質的捕蟲效能及其成分。現在，已經發現此誘引物質為一揮發性氣體。往後將會設法增加其誘引氣體的產量，並使用氣相層析儀分析之。最後希望可以將此物質應用到微生物防治上，期望能解決台灣松樹被線蟲感染的問題。 In Taiwan, all of the pine trees have one common problem - nematodes, which causes diseases in plants. And this experiment focuses on the nematodes’ natural enemy - nematophagous fungi and its “peculiarly caused mechanism.” When nematodes appear near nematophagous fungi, the latter will produce some substance to tempt the former. To investigate this alluring substance, a series of experiments are done and systematic steps are taken. The first finding is that this substance is a volatility gas. Later in this research, measures will be taken to make “rematophagous fungi” produce more of this gas. And “gas chromatograph” will be used to analyze this gas in the future. Finally, the possibility of applying this substance to the defensive measure of microbiology will be discussed.
光子晶體合成、特性與應用
We report an investigation on the synthesis, characterization, and application of photonic crystal. In the study of the synthesis of SiO? nanoparticles for the building blocks of photonic crystal, it is found that by changing the concentration of NH3 solution, we are able to control the size of SiO? nanoparticles. After trying several different methods, we discover that the vertical substrate method is the best way to arrange nanoparticles into a periodic structure. From scanning electron microscope, we confirm that SiO? nanoparticles can form a three dimensional hexagonal photonic crystal. From transmission experiment, we find that the wavelength of the minimum transmission is proportional to the size of nanoparticles. This result implies that using photonic crystals we can control the behavior of electromagnetic wave. Finally, we fabricate CdS nanoparticles on the top of photonic crystals with different diameter of SiO? nanoparticles. Using photoluminescence measurements, we show that by controlling the lattice constant of a photonic crystal the luminescent efficiency of CdS nanoparticles can be substantially enhanced. Out results, therefore demonstrate that photonic crystals are very important for the application of light emitting devices. 本研究主要是著重於探討光子晶體合成、特性分析及其應用。在有關合成光子晶體之奈米二氧化矽顆粒方面， 發現在合成過程中利用氨水的溶量可以控制顆粒的大小。在將奈米顆粒排列成光子晶體的研究中， 嘗試了多種方法後， 發現垂直基座法為最快速有效的方法。從掃瞄電子顯微鏡的觀測， 證實奈米顆粒是以六角對稱排列成整齊的光子晶體。在光子晶體的特性分析中， 利用光穿透實驗， 發現電磁波穿透率最小的波長與奈米顆粒成正比關係， 這顯示出可以利用光子晶體來控制光的行為。最後，本研究將光子晶體與硫化鎘奈米顆粒結合，經由光激螢光譜， 證明光子晶體確實可以增進物體之發光效率,這對發光元件的應用， 將有很大的幫助， 可以節省大量的能源
昆蟲模擬-雙振翅翼
本研究的目的在於探討蜻蜓兩對翅膀在不同的相位差之下對升力有什麼影響。在觀察蜻蜓及察閱相關網站、研究後發現蜻蜓前後翅的相為差有相差0.5 週期、相差0.25 週期、同週期三種不同振翅方式。在界定欲實驗的種類和評估現有的能力及資源後，決定研究加上相差為0.125 週期的四種振翅方式，於無風條件、相同的振翅頻率下進行實驗，測量其升力的變化週期。測量結果參照前人的文獻後發現，0.5週期產生的升力雖最小，但最平穩，所以為蜻蜓最常用的飛行方式。而0.25 週期升力會疊加，往下的力被抵銷，故為向上加速時使用。 ;The purpose of this study is to investigate the phase-shift between the front-pair and rear-pair wings on the maximum lift of a dragonfly. As observing the flight of a dragonfly and the literature survey from web sides, it has been observed that the general phase-shift modes of the dragonfly are in-phase-shift, 1/2 period and 1/4 period. It has been decided to include a 1/8 period phase-shift mode into the known three modes under the no wind condition with a fixed flapping frequency, the cyclic lift force of the dragonfly wing model has been measured. When it is flapping, we put the model on an electronic scale for measuring the weight of the model. After that we minus the original weight of the model, knowing the increasing or decreasing weight and the extra weight is the lift force. The results show that 1/2 period phase-shift mode produces the least lift force; however, it is the most stable flight, and is being adopted by the dragonfly for level flight. The in-phase-shift mode can produce more lift force on the flapping processes. The 1/4 period phase-shift mode produce the most acceleration, being adopted by the dragonfly for the climb flight.
以阻抗匹配調整太陽能最大功率輸出之研究
In recent years, the price of the oil keeps rising continuously. As a result, the prices of the commodities are rising, too. But what does this mean? This situation stands for the resources on are becoming more and more valuable. A few months ago, I read a Weekly Business Magazine and a Scientist Magazine. They both pointed out that the resources such as oil would disappear after fifty years, and that was a horrible phenomenon. Since almost everything in our modern lives are related to oil, like automobile, motorcycle and air plane. They all need oil for its ingredient. Even plastic bag plays a part in the products of it. I really cannot imagine what it would be like if we don’t have oil after fifty years. Owing to the green house effect is becoming more severe, there are many substitution resources found, such as hydraulic power, wind power and solar energy…etc. What we’re discussing in our topic is how to enhance the power of solar energy, because for now, we all know that the solar module is very expensive and it cost a great deal of money just only one square meter, but the price of module converted from sunlight or heat energy can’t be higher. Therefore, the work is mainly to design and carry out a solar max power point track. 近年來，油價不斷的上漲，連帶著民生的物價漲幅也是越來越可觀，但…這意味著什麼 呢??這所代表的是地球上的資源可以說是越來越珍貴了，前陣子曾經看過商業週刊、科學人 雜誌…他們紛紛所指石油這種能源可能在五十年後就消失殆盡，這個可是非常可怕的結果。 鑑於溫室效應愈趨嚴重，許多的替代能源紛紛出籠，像是風力、水力和太陽能等等。我們這 次的主題是在討論如何提高太陽能效率，現在的太陽能模組我們都曉得非常的昂貴，但是由 太陽轉化成電能的效率卻是低的可以。所以本作品主要目的在於設計與實現一個太陽能最大 功率追蹤器。
口蹄疫病毒鞘蛋白rVP1 誘發Prohibitin 之遷移
細胞凋亡具有控制生物體細胞數目之功能，能讓特定的細胞走向死亡，因此若能掌握其作用機制，便可能藉由調控細胞凋亡的發生，進而應用於癌症治療。前人研究（2.）指出，經基因重組技術純化之口蹄疫病毒鞘蛋白rVP1，會導致BHK-21的Akt 蛋白質去活化，引起細胞凋亡的現象。然而在其後續的研究中，卻發現到在BHK-21中大量表現磷酸化的Akt 蛋白質，並無法反轉由rVP1 所誘發之細胞凋亡。因此本實驗利用二維蛋白質電泳，尋找Akt pathway 以外之細胞凋亡相關蛋白質。目前已證明Prohibitin 此一蛋白質，在由rVP1 所引起之細胞凋亡中，有自細胞核移動至細胞質的現象。此外，亦經由實驗排除Prohibitin 位於Akt pathway 的可能性。Western Blot 之結果更顯示，經rVP1 處理後，Prohibitin 在很短的時間內便出現遷移的現象，故推測其具有調控細胞凋亡上游反應的功能。Apoptosis can lead some specific cells to programmed death, thus, it is a major way for creatures to control their cells amounts. If we can command the mechanism of apoptosis, we may use it as a therapy for cancer by artificial regulation of apoptosis. VP1 is one of the capsid proteins of Foot and Mouth Disease Virus (FMDV). A research (2.) has indicated that the recombinant VP1 (rVP1) can result in dephosphorylation of Akt in BHK-21, and then lead the cells to apoptosis. However, in their follow-up experiments, they discovered that even if they expressed great amount of phospho-Akt in BHK-21, it still couldn’t reverse the apoptosis induced by rVP1. Therefore, this experiment takes the advantage of two-dimension protein electrophoresis (2D) in order to find apoptotic proteins excluded from the Akt pathway. I have found that Prohibitin exports from nucleus to cytosol after rVP1 treatment. Furthermore, I eliminate the possibility that Prohibitin’s may be located in Akt pathway. The results of Western Blot also shows that protein amount of Prohibitin in BHK-21 increase after rVP1 treatment, hence the purpose of nuclear export of Prohibitin might not be to degrade it. It might have some much more important function in the process of exportation. Besides, Prohibitin exports to cytosol in quite a short time after rVP1 treatment. According to this phenomenon, I suppose that Prohibitin has a role as a regulator of apoptotic up-stream reactions.
翻轉「膜」力
The starting point of this experiment is to study the structure of soap-film. By changing the height of the triangular prisms, cuboids and pentagonal prisms, I observed the patterns set by the soap within the frameworks. It is surprised that when the proportion of prism is in a specific range, the phase in the middle of the structure will overturn 90 degree and then transmitted into another kind of balance pattern. I named this process “phase transition”. According to the experiment ,we can conclude the change of film patterns within variable prisms are all applied to this regular cycle:：
We know the soap films are forever attempting to minimize their energy. It stands to reason that surface tension tend to set up the film in its minimal surface. From the point of Mathematic, each structure should have only one single balance pattern, which is set up on the base of Fermat point and this pattern should stand to the minimize of it’s energy. However, we discovered that in some specific cases, one structure can allowed two kinds of balance films-patterns to exist. In these cases, any small vibration can cause the happening of “phase transition”. To sum up, I presume some structures have two different types of balance film-patterns: one of which stands to the local minimum (in this condition the pattern’s surface area isn’t the smallest); the other stands to the absolute minimum (in this condition the pattern’s surface area is the smallest).
There is an energy valley separate local minimum from absolute minimum. The second pattern (local minimum) will appear when the structure is blocked from attaining its absolute minimum, but surface intention is not powerful enough to support the film jumping over the energy valley. In this condition, if we works on the structure (such as blowing), which would provide the film of energy to cross the valley, and then phase transition take place. Vice versa, we can also force the film to jump from absolute minimum to local minimum and phase transition will occur as well. In a word, phase transition can happen in each two way, which connects the two types of balance pattern.
This report lays stress to find out the condition of phase transition. We also analyze the structure of soap-film by its included angles and surface area in hope to go deep into the science of soap-film.
我們實驗的出發點在於研究泡膜的立體結構。藉由改變正立方柱的高，觀察其平衡薄膜形式，意外的發現當正立方柱的邊長比在某個範圍時，泡膜結構中央會瞬間90 度翻轉，形成另一種平衡型式，我們將這個過程命名為面轉變(Phase Transition)。為了進一步了解面轉變發生的相關因素，我們設計了一連串的實驗，針對正三角柱、正四角柱、正五角柱、正六角柱發生面轉變的時機和條件分析討論。此外，我們還分析了泡膜結構中膜與膜夾角的特性、最小表面積和表面能之間的相關性，對於泡膜的立體結構做了一系列深入的探討。
月相變化對極地天氣系統的影響及其引發高緯度天氣變化之探討
本研究比較2003~2005 年，冬季及夏季西經168 度之「海平面溫度異常」對「緯度」的分布圖，結果呈現在赤道附近的變動較小，在高緯度地區的變動較大，為更精確的描述此變動，將每日高緯度地區的變動以「標準差」及「最大振幅」數量化，發現在南、北半球不論2003、2004或2005 年，每7~12 天南、北半球的「冬夏季海平面溫度異常」的變動變大，上述變化在北半球較為明顯，在南半球較不規則。 與2005 年冬季1 月31 日~3月17 日的北半球地面天氣圖進行分析比對發現，溫度的變動和極地天氣系統的形成關係密切，推測原因和冬季時極地為永夜，地面空氣溫度受到兩個跟月球有關的熱源影響較大，其一為海流和極地間每日有接近2 次的潮汐交替運動，其二為月球反射日光後投射到極地時，極地大規模的冰對月光的反照，對極地微弱的加熱作用。 針對2005 年6 月1 日~7月22 日之地面天氣圖作分析，結果發現夏季緯度的溫度變異和高中低緯度天氣系統的改變關係密切，推測此時和月球對海水的吸引使地表(特別在海陸邊界)的溫度，週期性的重新分配，或對大氣應也會造成週期性的質量分配有關。 以農曆為主重製溫度的變動圖並進行比對發現，無論2003、2004或2005 年，在月相為朔及望時常出現低值。此分析結果顯示，接近朔望時，高緯度海平面溫度有較穩定且變動較小的情形。而從天氣圖、農曆標準差綜合，所得結論為地月運動應和地球上高緯度天氣系統存在某種相關性。 ;Our topic is about how the moon phase variation influences the high-latitude weather system. This study analyzes the sea surface temperature anomalies (SSTAs), along 168°W in winters and summers from 2003 to 2005. The results show that the SSTAs varied relatively small around the equator and relatively large in high-latitude regions. So we next analyze the high-latitude SSTAs by the standard deviations, the maximum amplitudes, and the north surface weather graphs. After analyzing, the result shows that the SSTAs rise for every 7 to 12 days. Moreover, they relate to the winter high-latitude weather system very much while relating to the summer medium-and-low latitude weather system a lot. We think it’s because in winter eternal night, the sunlight won’t shine on the arctic zone easily. Other heat sources may become important: (1) the tidal movements between the ocean and the arctic zone. (2) The heat radiation of the moonlight and the moonlight reflection by the polar ice shields. But in summer, the moonlight effect becomes extremely small. The tidal force becomes the bigger influence factor: (1) the tidal attraction may distribute the temperature of the border between seas and land periodically. (2) It may cause the periodic atmosphere mass distribution. Additionally, after repeating the same experiment according to the Chinese lunar calendar, we found near full-moon and new-moon time, the SSTAs are more stable. Probably because of without the moonlight, the movements of rip-tides occur and help stabilize the polar weather conditions. In conclusion, the relative movement of the moon to Earth is likely providing a crucial heat source which will affect the high-latitude climate, and the heat source size probably influences the weather system cycle.
A Study For Heavy Metals Detection Using The Solubility change of Different Kinds Solute
A research on how a solution mixed with two or more solutes affect the solubility of each solute was done. If different solutes are put in a solution with a specific concentration, the solubility of the single state solute increases, stays the same, or decreases. The difference in solubility caused by several solutes will give solutions on selectively collecting heavy metals from bodies of water polluted by these heavy metals even more effectively. To test the changes in the solubility of the solution with several solutes, sedimentation has been used; however, colorimetric analysis was also done to observe the changes in color of solutes that show color in the solution. Nitrate compounds of Sodium, Magnesium, Strontium and Potassium were used for the typical metals, and for the transition metals, nitrate compounds of Silver, Cadmium and Lead were used. Test results show that Sodium decreased the solubility of Lead, and as the concentration of Sodium increased, the solubility of Lead increased too. On the other hand, K increased the solubility of Pb, and as the concentration of K increased, the solubility of Pb decreased. We can use the radius of ions and the enthalpy of hydration to explain the changes in solubility. In the case of Cd, as the concentration of K, Mg and Sr increase, the solubility of Cd increased with it. This is because the difference between the ionic radii of Cd and the other ions (K, Mg, Sr) has a tendency to increase in solubility in the enthalpy of hydration. Also, as the concentration of Ca increased, there was a tendency in which the solubility of Cd decreased. This is because the ionic radii of Cd and Ca are similar to each other and there was a tendency that the solubility decreased in the enthalpy of hydration. Through this experiment, the study shows the tendency of the change in solubility of several solutes by comparing ionic radius and the enthalpy of hydration, and if this result is used, the processing of certain heavy metals in the bodies of water can be even more effectively used than the previous method.