未代表出國作品

A pair of bacterial two-component system RssB-RssA was cooperated into Serratia marcescens for toxicity phenolic compound detection. First step of this study, E coli was used to accept the plasmid and certified by fluorescent. Then transfer the system from E coli into Serratia marcescens. Finally, 7 kinds of chemical, included phenol, benzene, toluene, xylenes, 4-chlorotoluene, 2-nitrotoluene, and kerosene, were used to check the sensitivity of this gene modified Serratia marcescens line. The results showed that this gene modified Serratia marcescens line had good performances and responses to those chemicals. 本實驗是以一受到二元訊號傳遞系統調控的發光基因重組質體，送入黏質色拉雷菌中，並以製備好的菌株進行毒性化合物之測試。在實驗的第一階段，我們將重組質體送入大腸桿菌內，並以其發光的有無來判斷是否達到送入的目的，其後再以電泳法確認各基因片段是否正確。第二階段再以相同的方法將選殖好的重組發光質體送入黏質色拉雷菌。第三階段，以發光重組菌針對酚、苯、甲苯、二甲苯、4-氯甲苯、2-硝基甲苯及煤油進行發光測試。結果方面，我們發現黏質色拉雷菌發光重組菌對於這一系列的酚環類化合物的確具有相當高的敏感度。

在綠色能源中太陽能的利用充滿了無限的機會，其中利用太陽熱能發電的太陽能煙囪觀念有很大的潛力。在本研究中，我們以實驗的方法討論煙囪高度、入風口大小、管型等因素對solar chimney 效益的影響，發現就原先的觀念設計中，有相當大的改進空間。整體實驗而言，所改變的各項變數中，以管型之改變有最大的改進效益。就同長度直管與漸擴管而言，漸擴管在風速上有明顯改進效果，且以流速與截面積換算成效率，較原直管設計之效率高出約20 倍，因此推測漸擴管應用在現行之solar chimney 以改進效能是極有可行性的。研究中也發現煙囪管壁的熱傳導也會影響氣體的總體流動，使效率降低。相信此實驗之數據對日後嘗試對solar chimney 進行改進的研究者有相當之參考價值。Solar energy has a great market potential among many clean energies. The new solar chimney concept using solar thermal power in power generation may have a bright future. Experimental work was performed to study the energy conversion characteristics of a solar chimney. The air moving capability and the influences of chimney height, the shape of chimney and air entrance dimension were studied. It was found that there is a great room to improve the current solar chimney design and thus the efficiency. Overall speaking, the chimney pipe geometry may have the most significant impact on the improvement of performance. A diverged pipe configuration can induce nearly 20 times more flow than a traditional straight pipe. Another finding is the heat transfer effect on the chimney pipe may have the effect in reducing the flow. It is believed that the present study0 is valuable for those people in designing a future solar chimney.

本研究主要是探討液晶面板在不同的電壓下，對紅外光區及可見光區之穿透光譜。藉由控制外加液晶面板兩側的電壓，改變內部的電場強度，驅使液晶分子長軸方向改變(偏轉)，以達到控制穿透率之目的。施加於液晶面板兩側的電壓V大於起始電壓V0時，液晶分子長軸受電場作用與電場方向平行，減弱引導偏振光扭轉之能力，讓部分光通過偏振片。令及分別代表穿透率達到最大穿透率之10%及90%時的外加電壓，則定義「光-電開關斜率」γ為：γ =(V90-V10)/V10。透射光強度與外加電壓關係曲線則稱為「光-電開關特性曲線」。穿透率除與液晶分子之旋光程度有關，我們也做了在不同電壓下，液晶分子之穿透光譜，並討論其特性。The main idea of the project is to discuss the transmittance spectra of liquid-crystal device in the range of infrared and visual light (400~900 nm) with different electric field by changing voltage. Different biases are applied to the liquid-crystal cell, causing the axis of liquid-crystal to rotate, and the transmittances are measured. If the application of bias is greater than the threshold voltage (V0), the axis of liquid-crystal will be parallel to the electric field, and make the beam pass through polaroid. Electro-optical switching slope γ is defined as γ =(V90-V10)/V10 , where V10 and V90 are the applied voltages enabling output light signal reaches up to 10% and 90% of its maximum intensity, respectively. It is understood that transmittance depends on the optical activity of liquid-crystal cells. Besides, we will discuss the relation between wavelength and transmittance of liquid-crystal cells.

1. 遊戲規則：將1～ 2m × 2n的連續正整數，由上而下、由左而右依序填入 2m × 2n的方格內。操作規則允許將2m × 2n做往右或往左或往上或往下的完全對摺，直到操作至所有單位方格均疊成一行，此同時有數字也由上而下形成一數列。2. 本研究即是探討操作完成的數列之數量與數字間的關連性。3. 我們發現：(1) 數列之數量與巴斯卡三角形有關。(2) 形成的數列必符合內文的 [ R(L) 性質]、 [ D(U) 性質]、[ R&D 性質]、[D&R 性質]。 1. Rules of thegame: Fill in order the continuous positive integers 1~ 2m × 2n, from top to bottom and from left to right in the 2m × 2n check. The operational rule allows a complete fold of 2m × 2n either rightward or leftward, or upward or downward, until all the check units pile up in a line. At the same time, all the integers form a series from top to bottom. 2. This study explores the relationship between the number of the series and the integers after the operation. 3. Our findings are: (1) The number of the series is related to Pascal triangles. (2) The series formed meet the properties mentioned in the study: [the property of R(L)], [the property of D(U)], [the property of R & D], and [the property of D & R].

高血壓所誘發的機械性展延是造成心肌肥大的基本因子，本實驗即藉由微陣列基因分析法同時大量的分析機械性展延所造成心肌細胞的基因表現。將新生鼠的心肌細胞施以 20﹪的機械性展延，抽取其 mRNA製作成 cDNA 探針與現成的 cDNA 晶片進行雜漬反應 （此晶片上包含了 480個如訊息傳遞、控制細胞生長週期、細胞骨架等的已知基因），在眾多有因為機械性展延而造成基因表現差異的基因中，我們選擇了 eNOS 基因（內皮細胞 NO合成?）進行西方墨點法及 NOS活性和 NO 產生量測定的實驗，進一步證實 eNOS 的基因表現量的確是增加的，此一結果與微陣列基因分析所得之結果不謀而合。 Mechanical stretch induced by high blood pressure is an initial factor laeding to cardiac\r hypertrophy. The use of cDNA microarrays has made it possible to simultaneously analyze\r stretch-induced gene expression in cardiomyocytes. Neonatal rat cardiomyocytes were cultured on\r malleable silicone dishes and were stretched by 20%. We compared the transcript profiles of\r cardiomyocytes under mechanical stretch for 60 minutes by hybridization of cell-derived cDNA to\r DNA probes immobilized on microarrays. The microarrays contained probes for 480 known genes\r including signal transduction, cell cycle regulators, cytoskeleton and cell motility, and so on. Eighteen\r genes were indentified that showed significantly differential expression in response to mechanical\r stretch in cardiomyocytes. Of the represented genes expressed, endothelial nitric oxide synthase (eNOS)\r genes was the most interesting one. Northern blot and western blot analysis further quantified the\r expression of eNOS gene. Mechanical stretch also increased constitutive NOS activity and NO\r production. Our results indicate that mechanical stretch induces eNOS gene expression thus increases\r constitutive NOS activity and NO production in cardiomyocytes.

線蟲為植物發生病蟲害感染的病源之一，而台灣的松樹，目前正面臨著松材線蟲入侵的危機。從文獻的探討中，發現線蟲有其自然界的天敵 － 線蟲捕捉菌。本實驗著重在探討線蟲捕捉菌特殊的捕捉機制。當線蟲捕捉菌附近出現線蟲時，會展生誘引線蟲的物質，並設計了一步步的實驗，去探討此誘引物質的捕蟲效能及其成分。現在，已經發現此誘引物質為一揮發性氣體。往後將會設法增加其誘引氣體的產量，並使用氣相層析儀分析之。最後希望可以將此物質應用到微生物防治上，期望能解決台灣松樹被線蟲感染的問題。 In Taiwan, all of the pine trees have one common problem - nematodes, which causes diseases in plants. And this experiment focuses on the nematodes’ natural enemy - nematophagous fungi and its “peculiarly caused mechanism.” When nematodes appear near nematophagous fungi, the latter will produce some substance to tempt the former. To investigate this alluring substance, a series of experiments are done and systematic steps are taken. The first finding is that this substance is a volatility gas. Later in this research, measures will be taken to make “rematophagous fungi” produce more of this gas. And “gas chromatograph” will be used to analyze this gas in the future. Finally, the possibility of applying this substance to the defensive measure of microbiology will be discussed.

古籍上記載山藥益肺氣，養肺陰，且最近研究報告發現山藥含有珍貴的dioscorin，其為山藥貯存養分的重要蛋白質，有抑制胰蛋白水解?之活性。而塵?糞便、屍體中的消化蛋白?會破壞呼吸道上皮細胞緊密連接處引起過敏氣喘反應。我對此甚感興趣，進而利用山藥的萃取蛋白dioscorin、塵?粗萃取蛋白及呼吸道上皮癌細胞A549，藉細胞培養、免疫螢光染色、螢光顯微攝影來實驗山藥是否真能保護上皮細胞緊密連接處。再利用膠體電泳、西方墨點法中抗體的高專一性、二抗的高靈敏度來確知我之前的實驗。另外也用ELISA 來實驗dioscorin或塵?引起A549 發炎的情況是如何。由實驗得知，dioscorin 可抑制及預防塵?引起的過敏氣喘反應；而經由ELISA 實驗發現，dioscorin 對於塵?刺激A549分泌發炎物質Eotaxin 並沒有助長或是降低的效果。It was believed that Chinese yam have benefits in treating asthma. Recently, it was found that the valuable dioscorin can be isolated from Chinese yam. The major function of dioscorin is nutrition-storage, however, it also have activities in inhibiting trypsin-like protease. This inhibitory activities trigger my interests. Because the major allergens, mites, and their stool may destroy the tight junctions of airway epithelium cells through their trypsin-protease activities. I therefore carry out an in vitro study to identify whether dioscorin can be used in protecting epithelium from the attack of mites. My results showed that dioscorin can protect the tight junctions of A549 cells from the attack of mite crude extract protein. I believed that dioscorin can be a good candidate in pharmacology application. The genetic and proteomic information are my further focus. On the other hand, we also investigated the possible activity of dioscorin in inhibiting mites-induced inflammation through using A549 as a model and employing ELISA. We found dioscorin neither inhibited nor enhanced the eotaxin being secreted from A549 under the stimulation of mites.

We report an investigation on the synthesis, characterization, and application of photonic crystal. In the study of the synthesis of SiO? nanoparticles for the building blocks of photonic crystal, it is found that by changing the concentration of NH3 solution, we are able to control the size of SiO? nanoparticles. After trying several different methods, we discover that the vertical substrate method is the best way to arrange nanoparticles into a periodic structure. From scanning electron microscope, we confirm that SiO? nanoparticles can form a three dimensional hexagonal photonic crystal. From transmission experiment, we find that the wavelength of the minimum transmission is proportional to the size of nanoparticles. This result implies that using photonic crystals we can control the behavior of electromagnetic wave. Finally, we fabricate CdS nanoparticles on the top of photonic crystals with different diameter of SiO? nanoparticles. Using photoluminescence measurements, we show that by controlling the lattice constant of a photonic crystal the luminescent efficiency of CdS nanoparticles can be substantially enhanced. Out results, therefore demonstrate that photonic crystals are very important for the application of light emitting devices. 本研究主要是著重於探討光子晶體合成、特性分析及其應用。在有關合成光子晶體之奈米二氧化矽顆粒方面， 發現在合成過程中利用氨水的溶量可以控制顆粒的大小。在將奈米顆粒排列成光子晶體的研究中， 嘗試了多種方法後， 發現垂直基座法為最快速有效的方法。從掃瞄電子顯微鏡的觀測， 證實奈米顆粒是以六角對稱排列成整齊的光子晶體。在光子晶體的特性分析中， 利用光穿透實驗， 發現電磁波穿透率最小的波長與奈米顆粒成正比關係， 這顯示出可以利用光子晶體來控制光的行為。最後，本研究將光子晶體與硫化鎘奈米顆粒結合，經由光激螢光譜， 證明光子晶體確實可以增進物體之發光效率,這對發光元件的應用， 將有很大的幫助， 可以節省大量的能源

本研究從理想城鎮(Ideal City)街道開始，討論平面上相異n 點到某一點的最短距離和。經研究後發現：當n 為偶數時，則到相異n 點的最短距離和所形成的區域可能是一個點、一個線段或是一個矩形；當n 為奇數時，則相異n 點的最短距離和所形成的區域將會退化成一個點。此外，本研究將理想城鎮的街道換成正三角形的街道幾何平面，同樣是討論平面上相異n 點到某一點的最短距離和。經研究後發現：當n 為偶數時，則相異n 點的最短距離和所形成的區域可能為一個點、一個線段、一個四邊形、一個五邊形及一個六邊形；當n 為奇數時，相異n 點的最短距離和所形成的區域則可能為點、三角形的情況。假使考量各點重要性的比重，分別加權後再求最小點。研究發現無論在理想城鎮或正三角形幾何平面上，皆可將各點視為多個權數相同之點重疊於此點上，便可利用先前的方式求得最小點區域。透過這次的研究，可以利用n 個相異點到某一點的最短距離和實際應用在貨物運送的問題或是消防設施配置等問題。The present study was intended to start with the Ideal City and proceed to discuss the sum of the shortest distance between a point and n different points on a plane. After the discussion, it was found that if n is even, the formed region could be a point, a line segment, or a rectangle. If n is odd, then the formed region must be a mere point. Further, the current study transformed the Ideal City into the geometric plane of an equilateral triangle. Similar to the previous discussion, if n is even, the formed region could be a point, a line segment, a quadrangle, a pentagon, or a hexagon. On the other hand, if n is odd, then the formed region could be a point, or a triangle. The result of this study, which investigated the sum of the shortest distance of a certain point to n different points can be applied to the real life situation, such as transporting goods or distributing fire control facilities.

掃描式穿隧顯微鏡(Scanning Tunneling Microscope)，是在奈米尺度下觀察物體表面的一大利器。但一台精密的STM，造價動輒數百萬元新台幣，本研究嘗試利用簡單、便宜的材料，製作一台簡易的掃描式穿隧顯微鏡。眾所皆知：掃描式穿隧顯微鏡的關鍵，就是量子力學中的穿隧效應原理，及壓電材料的應用，所以這些主題都是本實驗涉及的內容。除此之外，我們也運用簡單的物理原理，取代價格高昂的儀器，例如：利用彈簧代替昂貴的避震系統等。此研究利用穿隧效應的原理，並進而利用電腦得知樣品表面的結構，以達顯微鏡觀察分析物體之功用。 ;The major purpose of our project is to assemble a simple scanning tunneling microscopy with cheap and available materials for ordinary senior high school students. For example, we use springs for damping instead of electromotive force device. Weweld all the electronic circuit boards for ourselves in order to not only lower the cost but also inspect the inner structure more clearly and precisely. After the microscopy is assembled, we will use it as an appliance to observe the samples of nano-particles. We must repeat this procedure several times so as to make sure that the images we’ve gained is true, not just some disturbances produced by the environment. Finally, the microscopy can be applied to research fields as well as academic courses.