摘要或動機

Steroid hormones are very important for physiological homeostasis, but some functions of steroids are still unclear during embryonic development. Embryonic cell movements are required forming embryonic body. Recently, there is already known Pregeneolone (P5) which could affect epiboly movement of zebrafish embryos is the first product of the steroidogenesis pathway, but effects of further downstream products on epiboly movement are unknown. In order to know this, we treat embryos with Pregnenolone (P5), 17α-Hydroxypregnenolone (17OH-P5), DHEA, Progesterone (P4), 17α-Hydroxyprogesterone (17OH-P4), 11-Deoxycortisol (D), or Testosterone (T). We found out that P5 can accelerate epiboly movement, 17OH-P5 and D have no significant effects on it, and DHEA, P4, 17OH-P4, and T can decelerate it. These results indicated that steroids play important roles on embryonic epiboly movement in zebrafish. 類固醇荷爾蒙對生理平衡很重要，但其對於胚胎發育的影響仍舊不明。胚胎個體的形成需要胚胎細胞進行不同的移動排列。目前研究已知類固醇荷爾蒙生合成機制的第一個產物pregnenolone (P5)對斑馬魚胚胎epiboly 移動有影響，但其它更下游的類固醇荷爾蒙對epiboly移動的影響仍然未知。為了了解類固醇其他下游產物對魚卵早期細胞移動的影響，將胚胎處理Pregnenolone (P5) 、17α-Hydroxypregnenolone (17OH-P5) 、DHEA 、Progesterone (P4) 、17α-Hydroxyprogesterone (17OH-P4)、11-Deoxycortisol (D)、Testosterone (T)，發現17OH-P5、D對epiboly 的移動沒有影響，而DHEA、P4、17OH-P4、T 會使其變慢。這些結果說明了類固醇對胚胎細胞的移動扮演著重要角色。