摘要或動機

我們成功的用溶氣計偵測到了聚球藻 RF-1 在 28℃下光合作用的概日韻律。和傳統的研究方法比起來，這個方法具有連續偵測的優點，減少因不斷取樣所造成的影響，此實驗可觀察到聚球藻 RF-1 溶氣量之變化圖與一般藻類（如單殼縫藻）不同.在光 /暗條件下 RF-1 之溶氣量的增加與減少均呈週期性變化，而且此變化現象在進入連續照光後仍然可以維持兩個循環以上，這些結果顯示以溶氣計連續偵測聚球藻 RF -1之概日韻律是可行的，而且所得到的變化圖形遠比傳統方法（於不同時間取樣）所得者自然。本實驗同時發現含聚球藻之培養液的溫度，在進入黑暗週期時會有明顯的上升，由於其變化程度比其他藻類明顯，如加以探討應有助於對此藻以及其韻律特性之瞭解。We successfully detected the photosynthesis circadian rhythm of the prokaryote Synechococcus RF-1 under 28℃ by a DO (Dissolved-Oxygen) meter. The advantage of this method, comparing with the traditional methods, is that it can detect signals continuously, reduce the influence of discrete sampling. The DO curves of the Synechococcus RF-1 are different from that of other algae. Under Light/Dark conditions, the DO values of RF-i increased and decreased periodically. The periodic phenomena progressed over two cycles under constant lighting conditions. These results revealed the feasibility of using DO meter to continuous detect the circadian rhythm of the Synechococcus RF-1 The detected DO curves looked more natural than those obtained in the traditional discrete-sampling method. We also found that the temperature of the culture increased in dark cycle. Since the variation is clearer than that of other algae, further investigation will benefit the understanding of the Synechcoccus RF-i and its circadian rhythm.