Study of regenerative and ontogenetic processes under the influence of EHF EMR.
The increased sensitivity of aquatic organisms to the effects of EMF has been proven by numerous experimental studies. It has been repeatedly noted that exposure to EMF of certain frequencies and intensities leads to disruption of physiological functions, orientation in time and space, changes in the behavior of organisms, suppression of motor activity. Other ranges of electromagnetic radiation, on the contrary, can cause the effects of increased regeneration, growth rate and survival. In connection with these trends, the purpose of our research is to analyze the effects of the influence of electromagnetic radiation of extremely high frequency on the development of the Xenopus laevis and the regeneration of newts and planarians
Synthesis of fluconazole analogues with focusing on resistant strains Candida
Fungal infections, particularly those caused by resistant strains like Candida auris and Candida glabrata, pose a significant threat to global health. The widespread use of azole antifungals, such as fluconazole, has driven the emergence of multidrug-resistant strains, undermining the efficacy of existing treatments. These challenges necessitate the development of novel antifungal agents with enhanced activity and reduced resistance profiles. To address resistance mechanisms, we designed and synthesized hybrid molecules combining triazole and thiazolidine-2,4-dione (TZD) pharmacophores. This strategy leverages dual mechanisms of action: inhibiting fungal CYP51, a key enzyme in ergosterol biosynthesis, and disrupting fungal cell wall integrity. The structural versatility of hybrid molecules allows for targeted modifications to enhance antifungal potency, binding specificity, and pharmacokinetics. Using a stepwise synthetic approach, triazole-containing piperazine derivatives were first prepared and coupled with TZD-based carboxylic acids via optimized condensation reactions. The structures of the synthesized compounds were confirmed through advanced spectroscopic methods, including 1D/2D NMR and high-resolution mass spectrometry. The antifungal activity of these hybrids was evaluated in vitro against clinical and reference strains of Candida spp. and Aspergillus fumigatus. Among the synthesized compounds, 6a demonstrated notable activity against Candida parapsilosis (MIC 0.06 μg/mL), comparable to voriconazole. Compound 4b exhibited moderate activity against C. parapsilosis (MIC 1–2 μg/mL) and A. fumigatus (MIC 8 μg/mL). However, most compounds showed limited efficacy against highly resistant strains such as C. albicans 8R and C. krusei. This study highlights the potential of hybrid triazole-TZD molecules in overcoming resistance and improving antifungal efficacy. While promising, further optimization is required to broaden the spectrum of activity and enhance efficacy against multidrug-resistant pathogens. These findings contribute to the growing field of antifungal drug development, emphasizing hybrid approaches as a viable solution for combating fungal resistance.
Non-invasive study of the electrical activity of the brain of various chordate animals
In clinical practice, EEG is used to diagnose a number of neurological diseases and to diagnose epilepsy. But at present, the question of the nature of EEG has not been completely resolved and is of great scientific interest. There have been no studies at all on the non-invasive study of the electrical activity of the brain of the shark superorder, which belongs to the class of cartilaginous fish. By studying the electrical activity of the brain of various gnathostomes, it is possible to obtain an answer to the question of the emergence of rhythms from the point of view of phylogenesis and evolution, and by comparing their EEG with the human EEG, one can identify similar patterns that help in the study of reactions to various influences. During the work, for the first time, EEG indicators of spotted cat sharks, ECG, heart rate and respiratory rate of cat sharks and toads were obtained. In the future, it is planned to assemble a smaller neuroheadset for non-invasive studies of the electrical activity of the brain of small animals (sharks, toads, monitor lizards). This data can be used for evolutionary and medical research. *No animals were harmed during or after the experiments.