Estimated colony size is a basic work to count population of social insect up. It is not easy to measure any natural population accurately, in this respect, social insects here some advantages and some disadvantages over non-social ones. Previous studies have focused largely on the experimental colony of ants, such as Lasius niger, Myrmica sabuleti and Formica rufa, in the artificial nest. Furthermore, base on the division of labor, traditional Mark-Recapured Methods can not be used to measure complete colony of ant correcty. Here, we show that new method has be set up to calculate the colony of the ground black-giant ant, Camponotus fruedae,with a new indicator, ant hill. Predictions about the division of labor introduced to amend the formula of estimated population are discussed.本實驗探討黑巨蟻的蟻丘之形狀和生物意義,並分析利用何種方法能估算最準確的聚落大小。利用幾何圖形證明黑巨蟻會築似圓的蟻丘,並利用標識捕捉法的Petersen method(又稱為Lincoln index)、Schnabel method 和蟻丘大小重量,來推算黑巨蟻聚落之大小。發現黑巨蟻會築似圓度90%的蟻丘,而且標識捕捉法的Petersen method(又稱為Lincoln index)、Schnabel
method 並不能精確的推算出黑巨蟻之聚落大小;應使用黑巨蟻之蟻丘的重量當作參數來推算,才不會忽略幼蟲的存在。
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