本實驗針對一取自台北縣烏來南勢溪下游之溫泉菌，進行微生物學、生物化學、分子生物學三方面之觀察與實驗，期能得到一可生產特定酵素之菌種，且具耐高溫之抗逆境能力。對其酵素進行定性，並嘗試轉殖出相關基因，使之可大量表現。目前已篩選出一種可生產多種酵素之菌種〔依其採集地點暫稱WL-A12〕經菌種鑑定為Bacillus subtilis WL-A12。藉由菌落檢測法以及Zymogram 的方式做酵素分析，並以電導轉形等技術，希望能成功轉至E. coli 上表現。另外，也對該菌種作了一些基本微生物方面的觀察〔如需氧情況、最適生長溫度〕。We isolated enzyme-producing thermophilic bacteria from hot springs near downriver of Nan-shi, Wulai, Taipei (北縣烏來南勢溪). Through microbiological, biochemistry and molecular biological analyses, a multiple enzyme-producing Bacillus subtilis strain, designated WLA12, has been isolated. The growth condition of WLA12 was observed. Using basic colony assay and zymogram analysis (gel electrophoresis) to observe the expressed enzymes, molecular weight and gene size of the enzymes were revealed. With comparison to E. coli control strain, the related enzymes were only found in WLA12. To express the Bacillus genes in E. coli, molecular cloning and gene transformation via electroporation was carried out.
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