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土耳其

Recovering bioethanol from fruit wastes

1. Purpose of the research: Recovering bioethanol from fruit wastes by using brewing yeast and enzyme Viscozyme L\r 2. Procedures: Four different experiments were conducted in our project.\r Experiment-1: Samples in RM by the addition of only S.Cerevisia.\r Experiment-2: Samples in RM broth meduim by the addtion of 100 μl/g ViscozymeL and S. Cerevisiae\r Experiment-3 : ViscozymeL with different concentration\r Experiment-4: to determenine whether the enzyme with high concentration inhibited the fermentation.\r Analysis of ethanol: HPLC method was used for the analysis;\r HPLC method: (high performance liquid chromatography)\r 3. Data\r HPLC measured initial and final concentrations of glucose and ethanol by the addtion of S. Cerevisiae.\r Initial glucose concentration and final ethanol concentration was the highest in Grape pulp 12 and 9 g/L respectively. Despite the glucose concentration was the lowest, the ethanol concentration was quite high (7 g/L) in the mixture with orange. The lowest ethanol was produced in the mixture with pomegranate.\r Concentrations of ethanol and glucose from the samples in RM broth meduim by the addtion of 100 μl/g ViscozymeL and S. Cerevisiae\r After the 24 hour incubation by the addition of enzyme glucose concentration incresed by 1, 4 and 2 respectively in sample 1, 2 and 3. The ethanol concentrations in comparison to first experiment were higher especially in the sample1 (grape pulp).\r Effect of ViscozymeL with different concentration on glucose production.\r The glucose concentration generally increased by the addition of 200 micro L of enzyme as the time proceeded. In grape pulp sample glucose concentartion didnt increased by the addition of 500 micro L enzyme.\r The remaning glucose and produced ethanol concentrations by the addition of viscozyme L with two different concentartion and S. Cerevisiae\r As the concentartion of enzyme added incresed the amout of ethanol also inreased in sample 2 and 3 but in the sample 3 the fermentation was inhibited.\r 4. Conclusions\r In this study sugars in fruit wastes that are regularly not recylecled were fermented successfully into ethanol by using S. Cerevisiae.\r Generally we get more ethanol from the samples when the enzyme was used. As the graph5 shows the ethanol concentration generally increased as the enzyme concentration increased. But especially in the sample1 (mixture with pomegranate) at 200 and 500 micro liter concentrations, ethanol production were 2 and 0 g/L respectively. In the light of this daha we conluded that the enzyme with high concentartion might inhibit the fermentation. When the activity of enzyme was inhibited by keeping the pretreated enzyme in boiling water, the fermentation restarted and recovered more ethanol; 8 and 12 g/L at the enzyme concentration of 200 and 500 microliter respectively.

USING IRON-STEEL INDUSTRIAL WASTE SLAGS AS A FERTILIZER

Steel slag, is received from iron and steel production facilities in Turkey, and is up to 25-30% from steel production. These couldn’t have been reclamation for any field of use and free stored in nature. When the structure of this slag examined, it was contained metallic formation (Fe2O3, Al2O3, MnO2, MgO2 etc.) and oxide compositions (SiO2, CaO2 etc.). The aim of this project is investigate metallic content of steel slag used as fertilizer in plants. For this purpose, slag is used to determine the physical and chemical characterization. Then, it is separated from metallic content by using shaking table and multi gravity separative (MGS) method. After these procedures, there are determinate 6 groups with different densities and different grit size. Element analysis was made of these groups by atomic absorption spectrophotometer (AAS) and ICP-OES system. In addition, mineral analysis was determined by XRD device. Thus, within each group minerals have been identified. Slag-soil mixture was prepared for determination the highest yield of using six different slag groups as fertilizer in the plant. Thus, corn crops were grown in different combinations. Then harvested corn plants and done soil analysis of the plants. As a result of experiments, percentage of the most effective slag-soil composition for corn was determined. The highest yield in the slag pot was determined the rate of %12.5 and coarse-grained. Ideally, the slag content found to be chinerals such as merwinite (3CaO-MgO-2SiO2), akermanite (2CaO-MgO-2SiO2), gehlenite (2CaO-Al2O3-SiO2). Thus, it was detected the steel slags can be used as feed material for plants. Hence by maintaining the ecological balance economic contribution to the country was achieved.

ELECTRONIC STUDENT-TEACHER POLL ATTANDANCE SYSTEM

Our goal is to make roll call systems at schools technological. While Rolling Call system is getting technological capacities some useful outcomes occur as well like; To remove the cost for class books by abolishing the class books used for roll call To eliminate mistakes with the usage of class book .(The numbers written incorrectly) To save again a large amount of waste paper( The short messages will be send to the parents instead of mailing the letters to the their adresses once a week about their students' absance.) To improve communication among school and parents ,by this way to prevent various kinds of problems arising from absenteeism. To make teachers school boards more efficently and motivated besides their affairs will get easier as well. To save time of school managers(They have no Works like saving the absenteeisms into e-school network because everything including all the absenteeism info will be carried into the system automatically) To save time of each lesson the teachers will not loose time for the roll call ,so they will be able to devote their time to their students and training. -Technological polling mechanism will contribute to education, this contribution will make all the students and teachers happy because all the teachers carry concern whether their curriculum will be finished or not through this system recording all the rests of teachers because of illnesses and some national celebrations.

Novel Biotechnological Approach for Recognition and Purification of Antibody: Lectin Affinity Membranes

Immunoglobulin G is a glycoprotein structured molecule that is produced by the immune system and protects organism from harmful effects of antigens. Ig G amount in the blood plasma is an appropriate indicator of; infection, cancer, diabetes, cardiovascular diseases, Alzheimer and other autoimmune diseases. Besides, purification of Ig G used in the treatment of these diseases from naturel sources is carried out at high costs on the World market. It is hard to obtain Ig G in high amounts and without any decomposes, that’s why it is important to develop new systems that will help to recognize and purify Ig G antibody. In this project, my purpose was; recognizing Ig G antibody with efficient, high amounted, fast, easily, with less toxicity, economically and purifying Ig G in high ratios from its natural sources. For this purpose p(HEMA-EDMA) membranes are synthesized with free radical photo polymerization method and characterized according to SEM images, swelling behaviors FTIR analysis and elemental analysis. In order to adsorb Ig G to polymeric membranes; polymeric membranes are activated with silanization agent (IMEO) and derivatized with Con A which is a lectin affinity ligand. In the SEM results it is examined that membranes are in spherical structures. Highest swelling value is determined as 224.8%.Binding of IMEO was demonstrated with FTIR and Elemental Analysis. Optimum conditions for Ig G adsorption to membranes are; 1.5 mg/ml initial Ig G concentration, 30 minutes of adsorption time, pH 4 citrate buffer 37 0C and without any different ion strength. Optimum adsorption capacity is determined as 253.8 mg/cm2 and it is also determined that this value is 7 times higher than nonspecific Ig G adsorption to p(HEMA-EDMA) membranes. Ig G adsorption-desorption cycles (5 times) proved that product is reusable without losing its adsorption capacity. According to the electrophoresis, Ig G could be desorbed in pure form without any denaturation to its structure.

FABRICATION AND CHARACTERIZATION OF CARBON NANOTUBE DOPED PHOTOVOLTAIC CELLS

Nowadays, the increase in population and the rapid depletion of nonrenewable energy sources brings the need for energy. In this case, scientists are forced to develop technologies by using renewable energy sources. Sun is the unlimited and renewable energy source. Organic solar cells absorb the light from the sun by the active polymer layer and transform it into electrical power. Organic solar cells are advantageous than inorganic ones because of being low-cost, easy-to-use and proper for large scale applications. In this project, it is aimed to produce organic solar cells by using specific amounts of carbon nanotube (CNT) doping. According to this aim, it is detected by using the fluorescence spectroscopy that CNTs can be used in organic solar cells. Later, the homogenous distribution of doping SWCNT into donor material was displayed by AFM, and correct proportion of SWCNTs are chosen by those images. In order to increase the efficiency of organic solar cell SWCNT doped P3HT was used as donor molecule. The acceptor molecule was PCBM in here. Surface characterization of prepared samples was made by Atomic Force Microscope (AFM), while electrical characterization of them is done with airless environment cabin (glove-box) system in nitrogen environment. As a result, devices prepared with addition of cyclohexanone in P3HT: SWCNT%:PCBM new load paths to carbon nanotubes were provided, as a result of the measurements short circuit current obtained was raised from the reference to 53%. The best yields were found as 2.24% in 0.2% SWCNT doped devices. This result shows efficiency is healed according to the reference rate as 64%. In this study, certain amounts of carbon nanotube doped organic solar cells were produced, which are highly efficient rather than traditional organic solar cells and low cost, easy-to-produce rather than inorganic solar cells, by using environmentally friendly materials.

SeedBot: Low-Cost Seeding Robot for Agricultural Applications

This paper presents a semi-autonomous seeding robot which is based on both electrical and mechanical platforms that perform advance agriculture process. SeedBot composed of four components: drilling mechanism, body of robot, seed container and paving mechanism. Other than those components the sensor system and the control system are also discussed. The aim of this study is designing and building a low-cost robotic system to automate and optimize process during farming especially for personal usage. This study demonstrates that semi-autonomous farming has crucial advantages over conventional farming. In addition to that, SeedBot provides safer, requires less manpower and precise farming than usual methods that we have so far.

Synthesis of Macro Porous Activated Carbon from Waste Polyethylene Terephthalate (PET) Bottles and Investigation of Usability in Dye Removal from Water Sources

Colorants are used in many industries, especially in the textile industry. These substances both cause visual pollution and create an anaerobic environment for aquatic creatures. In this study, it is aimed to examine the usability of activated carbon synthesized from waste polyethylene terephthalate (PET) bottles, which is an important environmental problem, in removing the pollution caused by the colorants caused by industrial activities in water resources.

New Approach to Food Packaging: Antimicrobial and Edible Materials

In addition to adequate and balanced nutrition in the protection of human health, the reliability of consumed foods is also of great importance. When foods contact with the environment, they are undergoing many microbiological, physical and chemical changes such as moisture loss, aroma exchange, oxidation and contamination with microorganisms. So this changes reduce quality and shorten the shelf life. During cutting and processing of chicken meat, especially contamination on the surface causes deterioration of the meat starting from the surface and thus short shelf life. Though contamination of chicken meat surface is inevitable, growth of contaminant microorganisms can be inhibited and microorganisms can be killed. In recent years, increased risks of infection due to antibiotic-resistant microorganisms have forced the discovery of new and natural antibacterial materials. It is a new and advantageous approach to avoid environmental pollution caused by the use of food packaging and safety of food, prolongation of product shelf life by natural, edible, antibacterial biomolecules in packaging products. In the scope of the project, in order to produce an edible alginate gel containing the antimicrobial peptide nisin, which has an antibacterial property to prevent microbial growth on the surface of chicken meat, following steps was done;  Synthesis of calcium alginate and nisin immobilized calcium alginate beads  Optimization of immobilizing nisin in calcium alginate beads,  Characterization of nisin immobilized calcium alginate beads by ATR-FTIR Spectrum and SEM Analysis,  Determination of the antimicrobial activity of white meat product chicken which is coated by nisin immobilizing calcium alginate gel.

THE DESIGN OF MICROFLUIDIC PUMP (MFP) FOR MEDICAL FIELD

The ability of microfluidic (MF) device technologies to provide a lot of information with a small amount of sample, the opportunities it offers increases their use in the medical field in the bedside monitoring in drug delivery systems. Three-dimensional (3D) printer technologies provide advantages such as cost-effectiveness in the production of MF devices and quick and easy production in intricate designs. In our project, it is aimed to design microfluidic pumps (MFP) to be used in the medical field and conduct its production with 3D printer technologies. The developed MFP is intended to be at low cost, bio-compatible, adaptable, and portable to the drug, suitable flow properties as a pharmaceutical pump. First of all, MFP air channel, flow channel, etc. parts were designed and printed with the help of a 3D printer and on AutoCAD, one of the professional drawing programs. The poly(dimethylsiloxane) (PDMS) membrane that will enable MFP activation is produced in different thicknesses and glued to the air channel of MFP. The resistance to the applied pressure is observed, and the appropriate membrane thickness is determined as ~ 235µm. Liquid PDMS was applied to the inner surfaces of MFP's air and flow channel, PDMS membrane was placed between them, and the parts were assembled in the oven at 60ºC. MFP has been connected to the pneumatic valve system, where operation codes have been prepared with Arduino Uno, and flow properties have been examined. The flow rate of MFP is ~ 50 µL/min at a maximum of 15 Hz, and the backpressure is ~ 0.085 Pa under a maximum pressure of 3 bar. Also, values such as size, membrane thickness, and applied pressure for the possible models of MFP were supported by theoretical calculations. As a result, MFP, which is biocompatible, drug adaptable, portable, wearable technology application potential, and has suitable flow characteristics as a pharmaceutical pump, has been developed. MFP introduced a microfluidic pump system that can make life easier for the patient and contribute to the national economy through domestic production and can be used as a drug pump in the treatment of diseases such as diabetes and cancer.

CONTACTLESS AND NON-DESTRUCTIVE DETECTION OF CHICKEN MEAT CONTAMINATION WITH LASER SPECKLE METHOD

Harmful microorganisms in food can cause deterioration of human health, poisoning and in some cases even death. Especially fresh meat and chicken products create a suitable environment for the growth of microorganisms in terms of the nutrients it contains, water activity and pH level. For this reason, detection of microorganisms in meat products is an important issue in terms of food safety and human health. In this project, it is aimed to detect live microorganisms in meat products, especially chicken meat, in a simple, non-destructive, non-contact and fast way using laser speckle method. Laser speckle images of healthy and stale chicken meat were taken, contrast parameter and correlation analysis of the obtained patterns were made. It was observed that the contrast parameter for staled chicken meat increased by approximately 3 times compared to fresh chicken. This increase provides an understanding of the difference between contaminated chicken and fresh chicken. Speckle density changes over time in relation to the movements of living microorganisms. Thus, the correlation in laser speckle density patterns taken from contaminated tissues is disrupted. In the measurements taken with photodiode, by analyzing the change of light intensity of the speckle patterns on fresh and contaminated tissues over time, the detection of microorganisms was made easier and more precisely without the need for image processing. The proposed measurement system is a new method that detects meat contamination with laser speckle imaging. It can be developed and made portable and can be used easily in homes. Since it is a simple, non-destructive and fast method, it can be used to determine the shelf life of meat in food distribution places and markets. In addition, it has the potential to be calibrated and used for other food products other than meat products. The system developed with this study is cheap and easy to use, and the laser speckle imaging method is used in a different field other than biomedical, contributing to the literature.