人類粒線體蘋果酸.可利用NAD+或NADP+為輔.，幫助腫瘤細胞獲得能量，但一般生理條件較傾向以NAD+為輔.。本研究將K346 修改成偏好NADP+之粒線體蘋果酸.家族中具有高度保留性的絲胺酸、及不具極性之丙胺酸，探討為何此酵素較偏好以NAD+為輔.。天然及突變株酵素的催化常數 (kcat)、基質Km 值、及抑制常數 (Ki) 測定結果顯示K346 之點突變不會影響基質Km 值，但K346S 之kcat明顯上升，繼而改變此酵素對NADP+之選擇性。本研究對於人類粒線體蘋果酸.催化機制的了解，有助設計具專一性的活性抑制劑，未來可應用於抑制腫瘤細胞能量來源，進而抑制腫瘤細胞生長。;Human mitochondrial NAD(P)+-dependent malic enzyme can help tumor cells gain energy, using either NAD+ or NADP+ as the cofactor, but prefers NAD+ as the coenzyme. By mutating the K346 to Ser, conserved in NADP+-dependent ME and to Ala with non-polar, we explore why human mitochondrial NAD(P)+-dependent malic enzyme prefers NAD+ as the coenzyme. We measured the proteins kcat, Km and the Ki values. The experiments showed that mutantions don’t affect the Km values, but K346S increased in the kcatvalue, transferring the coenzyme specificity to NADP+. If we develop deeper understanding of the human mitochondrial NAD(P)+-dependent malic enzyme, we can design a specific drag to inactivate the enzyme activity, and inhibit tumor cell growth.

這個專題研究的目的是要發展出一套簡單可靠的方法和廉價自製的器材，在地面上即能有效估測大氣層的厚度。我們小組研究光學中雷氏(Rayleigh)散射的原理，針對空氣分子對光線散射作用和特定方向之偏極效應，利用一已知散射長度之路徑，測量其偏極光的強度，同時比對由大氣層散射而來，在同一偏極面上的散射光強度，即能估算大氣層的厚度，方法簡單新穎，自製器材經實際測量和改進，有發展和推廣的價值。\r The main idea of the experiment is to set a system in order to effectively estimate the thickness of the atmosphere. On the theory of “Rayleigh Scattering” (small air molecules sizing about 10-4μm), we developed an equipment that has two tubes. The tubes lead the scattered lights from two paths. One is called “air light” scattered in the ground air, and the other “sky light” is scattered in the sky and reflected by a beam splitter. The two paths are on the same plane; the scattered lights are perpendicular to the direction of sunlight and 100% polarized. We could adjust and measure the distance “d” of the air light path. We simultaneously observe and compare the intensity of the lights from the two paths with the electronic instrument made by ourselves. By using the known distance “d” and the reflection “x” of the beam splitter, we can calculate the thickness of the atmosphere. The experiment is simple, novel and easy to do in an extensive field at school. Researchers don’t have to use a bloom, radar or satellite to discover the atmosphere, but you could use a simple equipment to observe the features of it.

The pharmaceutical industry is constantly searching for methods that allow drugs to be delivered as a direct response to a specific stimulus, in which the locus of delivery is in the vicinity of required region. A unique thermo?reversible hydrogel,F127 modified with dimethacrylate (DMA), that can deliver drugs at physiological temperatures was synthesized. Nanoparticles which are specific for targeting human body cancer cell were absorbed by this hydrogel. The toxicity of nanoparticles with different diameters and coating was measured using the MTT assay. It was found that nanoparticles with smaller diameters and folate coating were most toxic to the cancer cells. The release rate of the nanoparticles from the hydrogel was measured as a function of temperature with the hydrogel releasing approximately 3 L nanoparticles per hour. Exposing this drug delivery system to cancer cells would effectively inhibit MCF7 cell proliferation. By grafting this nanoparticle?loaded hydrogel onto a thermoelectric module, the release of the drug would be controlled. Thus, a successful temperature sensitive hydrogel was synthesized that releases cancer?targeting nanoparticles which inhibit cancer cell proliferation, thereby engineering a controlled drug delivery system.

Fluorescent proteins are used to label and tag tumor cells. These are also used in molecular cloning methods, and in monitoring cellular processes. However, the Philippines does not have a local source of fluorescent proteins, and thus imports these expensive chemicals from other countries. Possible sources of fluorescent proteins may come from marine organisms and certain plants. This work presents the screening, isolation, and characterization of fluorescent proteins from a potential local source—nudibranchs. The pigments of nudibranchs function to make their different body parts, as well as the cerata, brilliantly colored for defense and protection as in camouflage and aposematic behavior. The pigmentation in the skin of the nudibranchs was used as the fluorescent protein source. Crude protein extracts from the seven species of nudibranchs collected from Mabini, Batangas, Philippines were run through Reversed- Phase High Performance Liquid Chromatography (HPLC) for screening. Graphs of the absorbance of the seven species showed which species exhibit the most number of aromatic amino acids, the main component of fluorescent proteins. The species with the highest absorbance at 280 nm, which is Chromodoris willani, was run through the HPLC again for recovery and collection of fractions. The fractions were examined through fluorescence microscopy, wherein the samples were filtered at three emission ranges, the first at 450-470nm,the second at 515-545 nm, and the third at 600-650 nm, which corresponds to blue, green, and red, respectively. The proteins which emit at the said ranges were the only component allowed to pass through. The results, which are images of the excited proteins, showed that fluorescent proteins are present in the fractions of Sample 6—Chromodoris willani.

本實驗以薄膜色層層析（TLC）、高效能液相層析（HPLC）分析等化學方法，進行金銀花品種差異的鑑識；此外，配合生藥學的顯微鏡檢視，如中藥材組織鏡檢、藥材粉末鏡檢等比對，以期找出辨別金銀花品種的方法。研究至目前為止，由金銀花之薄膜色層分析的Rf值（0.225、0.425、0.7、0.85、0.95）可確認出金銀花藥材，並得知金銀花藥材中皆含有綠原酸的成分；以高效能液相層析的圖譜與成分峰的積分面積可用來判別金銀花的品種，並從質譜分析瞭解成分含量；進行生藥學的藥材粉末組織鏡檢，發現無法作為金銀花藥材的分類憑藉。未來將持續延伸實驗，朝向中藥奈米化與一般粉末在藥效、成分上差異的比較，並進行金銀花萃取液的抗菌作用試驗，瞭解不同品種之金銀花藥材在藥理作用的異同，接續著奈米化藥材的應用與實踐。Using thin layer chromatograph (TLC) and high performance liquid chromatography (HPLC), we can study how to differentiate the species variation of honeysuckle; beside, based on the observation of biopharmaceutical microscope, such as comparing the histology of Chinese herbs and its powder, we suggest that we could differentiate the species of honeysuckle. From the present, firstly, we could distinguish the honeysuckle from other herbs by the Rf value of TLC(0.225, 0.425, 0.7, 0.85, 0.95), from which we find that all honeysuckles contain the component of Chlorogenic acid. Secondly, we could tell the species of honeysuckle according to the map of HPLC and the peak area after integration, as well as the integrants of honeysuckle by way of LC-Mass analysis. Thirdly, while studying the histological analysis based on the observation of biopharmaceutical microscope, we found that it shows no difference between all the honeysuckles; thus, it fails to be a scientific method used to distinguish the herb honeysuckle. However, in the biochemical experiments of honeysuckles, we found honeysuckles from different sources and the place of origin shows difference in their antibiotic effect, showing the importance of local medicine. When it comes to my future work, in order to extend my experiments on honeysuckles, I would compare the nano-scale honeysuckle powder with normal-sized one in their clinical effects and components.

This chemistry project was designed to measure the vitamin C levels within liquid cold and flu remedies, and see if they had been accurately stated on the package. A range of Cold and flu drinks was selected, including one made with only cold water (Lemsip Original, Lemsip Max, Effer-C-Cold Water, Relief and Lemting) .The procedure used to carry out the experiment was a redox titration, each trial required two titrations, with the first being a blank titration to determine a sodium thiosulfate concentration in the absence of vitamin C. The following Drinks had more Vitamin C than stated on the packaging – Lemsip Original (19.4%), Lemsip Max (22.1%), Effer-C (17%) and Relief (8.8%). Lemting was the only drink made to directions that had less Vitamin C than stated by 31.8%. Two conclusions can be drawn from these results, the first being that Lemting has the most inaccurately recorded mass of vitamin C and is also the only drink with less vitamin C than stated. The other interesting result is that Effer-C (the only drink made with cold water) had 170mg of extra vitamin C. Of the other drinks that had more vitamin C, Lemsip Max was next with an extra 22.8mg. Early research had showed that Vitamin C was affected by high temperatures (above approximately 70oC) and, as cold and flu drinks are commonly made with hot water the Vitamin C levels might change when they were made up. This poses a further possible research question which is, in making cold and flu remedies with hot water is some of the available Vitamin C being destroyed?

The purpose of this experiment is to determine whether or not wood can be used as an alternative testing method for sunscreens and which species of wood are good for this objective. This will be accomplished by applying varying strengths, or SPFs, of sunscreens onto thin wood veneers. They will then be exposed using a weatherometer, in which real light and sun conditions will be simulated. Using a device called a Pulmac, the samples of wood will be pulled apart from the centre at zero span. Based on the consistency of the strengths, it can be determined how the wood reacts to the sunscreen and whether that species of wood is suitable as a testing medium. Trees are an important commodity which enrich the lives of many people, particularly those who live in British Columbia. They provide the means of living and recreation for numerous people, whether it is employment, business, housing, or camping. Since trees are a renewable resource, they are convenient and realistic to use for testing. There are two possible extensions to this project. If the active ingredients in sunscreens can be determined, then we may be able to use these elements to create a new type of stain for fences and other structures. This could make the necessity for painting less frequent, creating cheaper options for the homeowner. The second possible extension of this project is to use the results to as an initiative to reduce the amount of animal and human testing done by manufacturers. This is important, as animal testing not only causes unnecessary pain, but often the results are not very accurate. Overall, I determined that this method of testing sunscreens would work quite well, especially with the following species, listed in decreasing order. 1) Yellow Cedar 2) Yellow Poplar 3) Maple 4) Hemlock These results were based on how tightly grouped together the means of the strength values were for each species. The closer they were, the more consistent that type of wood and therefore the better they are for testing. Overall, I think this method of testing sunscreens would work quite well, particularly with the yellow cedar, maple, yellow poplar and hemlock, as they resulted in fairly consistent strength values. Generally, the sunscreens with a high SPF, or Sun Protection Factor, resulted in a lower strength loss than the sunscreen with SPF 15. In addition to proving that sunscreen is a good method for preventing UV rays from reaching the skin, this experiment has also confirmed that there are other methods of testing sunscreens, which can be researched further. Along with my main presentation I will be showing various graphs, statistics, and pictures. They will be supplemented by a booklet of “Commonly Asked Questions and Answers” and species descriptions for each type of wood.

1. 遊戲規則：將1～ 2m × 2n的連續正整數，由上而下、由左而右依序填入 2m × 2n的方格內。操作規則允許將2m × 2n做往右或往左或往上或往下的完全對摺，直到操作至所有單位方格均疊成一行，此同時有數字也由上而下形成一數列。2. 本研究即是探討操作完成的數列之數量與數字間的關連性。3. 我們發現：(1) 數列之數量與巴斯卡三角形有關。(2) 形成的數列必符合內文的 [ R(L) 性質]、 [ D(U) 性質]、[ R&D 性質]、[D&R 性質]。 1. Rules of thegame: Fill in order the continuous positive integers 1~ 2m × 2n, from top to bottom and from left to right in the 2m × 2n check. The operational rule allows a complete fold of 2m × 2n either rightward or leftward, or upward or downward, until all the check units pile up in a line. At the same time, all the integers form a series from top to bottom. 2. This study explores the relationship between the number of the series and the integers after the operation. 3. Our findings are: (1) The number of the series is related to Pascal triangles. (2) The series formed meet the properties mentioned in the study: [the property of R(L)], [the property of D(U)], [the property of R & D], and [the property of D & R].

灰色理論近來廣泛應用於各行各業的管理資訊科學上，本研究乃應用灰色理論的一階微分中輸入變數為一項之 GM ( 1 , 1 ）模型，來預測台灣中部地區土石流主要因素雨量的月平均量值。本文運用三筆以上的歷史資料，作最近一年歷史性資料的預測，藉以驗證獲得其誤差（±△e）之校止參考依據值，比利用殘差檢驗所得的誤差校止數據，更能修止出精確的預測值，接著再做台灣中部雨量未來之預測，以作為未來雨水、土石流防治之參考 · 為了能迅速產生所要的灰色預測值，研究中並以 VB 程式語言及以Petri-net分析其動態處理行為，藉 GM ( 1 , 1 ）模型的專屬處理支援程式，以做為未來雨輦自動化處理警示與預測之支援機制。Recently Grey Theory has been widely applied to management information science in all kinds of areas. This research applies the first Level differential of the Grey Theory to put a variable in GM( 1,1) model to forecast the monthly average value of the most common precipitation, in the midland Taiwan earth-stone flood zone. This paper uses more than three historical data to forecast the most recent year’s historical data, which can be used to verify and acquire referential correction data of its errors ( ±△e). The aforementioned method can correct forecast data more precisely than the differential inspection. In addition, this method can then forecast future precipitation in midland Taiwan earth-stone flood zone and the results can serve as reference for future precipitation control. To rapidly receive the needed Grey forecast values, the research analyzes its dynamic process method by utilizing VB program and Petri-net to build a dedicated process-support program of GM( I , 1) model. This program will serve as a support mechanism for future automatic precipitation warning and forecast.

NO.59-04 2020 AUG | 科學研習期刊目錄 本期專題 核心素養與數學素養 數學領域核心素養與數學素養之我見 | 李源順 咖啡豆的數據分析 | 洪士薰 燈謎與數學 | 李祐宗 有數學感的教與學：以比較型問題為例 | 李源順 教學現場 個案小學教師在理財情境中數學素養的專業成長 | 湯永麟 素養導向的數學課室樣貌―以國小「比與比值」的教學為例 | 溫世展 12年國教精神在國小數學課室之實踐樣貌 | 陳玉珊 從數學奠基進教室談素養教學─以「棋奕三視圖」為例 | 林柏寬 科學新知 單淘汰賽中的數學－高中數學的探究與實作 | 張宮明 摺出畢氏數 | 林靖捷 透過摺紙也可以學數學推理 | 譚克平 特約專欄 以建築為主題的跨領域探究學習 | 劉淑雯、陳韻如、褚希雯 炫光手持木鋼琴STEAM創意課程 | 儲述傑、丁于真、黃琴扉 下雨了「梅」？ | 江鈞、李竑慶、呂奕勳、林育慎、侯宜妤、林威帆、王嘉琪 森棚教官數學題 全數出動 | 游森棚 科普活動報導 週日閱讀科學大師 讓科學普及不再是夢想 | 李旺龍 讓思考可見－探究式教學遊戲化策略 | 陳佩雯、呂玉環、程詩婷、顏慈瑤、蘇萬生 「全國大專暨高中青年物理辯論競賽」真的只是物理辯論? | 余進忠、蘇萬生 總召集人的話 12年國教課綱強調透過各學習領域的分工與合作培養學生的「核心素養」，在分工上數學學習領域旨在培養學生「數學領域核心素養」和/或「數學素養」。本期特別以「核心素養與數學素養」為專題。 「本期專題」單元共有四篇文章，〈數學領域核心素養與數學素養之我見〉解說「數學領域核心素養」和「數學數養」個別定義與兩者關係。人工智慧很適合用來發展瑕疵咖啡生豆的挑選法，〈咖啡豆的數據分析〉即介紹如何使用簡單工具、運用高中數學中的知識，讓學生動手實作體驗數據分析與人工智慧。 〈燈謎與數學〉分享51則供讀者自學或教學的參考的數學燈謎。〈有數學感的教與學：以比較型問題為例〉介紹如何透過學生的親身經驗等，了解比較型問題的意義，以培養帶得走的能力，以及學習數學的感覺。 「教學現場」單元也刊登四篇文章，〈個案小學教師在理財情境中數學素養的專業成長〉分享一位小學老師在理財情境中數學素養之專業成長的探究經驗。〈素養導向的數學課室樣貌―以國小「比與比值」的教學為例〉分享自行設計的「比和比值」教學活動，結合數學感理論的數學課室實踐。〈12年國教精神在國小數學課室之實踐樣貌〉分享在施教「基準量和比較量」單元時，讓學生透過「同儕間『抓』出『錯』誤之處」的「抓錯」活動經驗。〈從數學奠基進教室談素養教學─以「棋奕三視圖」為例〉則分享以「棋奕三視圖」為例的數學奠基進教室活動經驗。 「科學新知」單元刊登三篇文章，〈單淘汰賽程安排中的數學〉分享在國內及國際獲獎專題研究「單淘汰制賽程分析」中的數學。〈摺出畢氏數〉分享「摺出畢氏數」課程、教具以及「畢氏數書籤」作品。〈透過摺紙也可以學數學推理〉介紹和數學推理有關的四個摺紙基本技巧。 「特約專欄」單元刊登四篇文章，〈跨領域課程發展與教學實踐初探〉分享作者和某國小合作推動跨領域課程發展與教學實踐的理念和作法。〈炫光手持木鋼琴STEAM創意課程〉分享可帶入STEAM實作、實現跨領域的知識統整與 應用的炫光手持木鋼琴教學活動。〈下雨了「梅」？〉探討梅雨季未來的降雨變化趨勢，並傳達氣候變遷下應有的防災資訊。〈森棚教官數學題：全數出動〉拋出1,11,111……分別被同一數值除後的餘數問題。 「科普活動報導」單元刊登三篇文章，〈週日閱讀科學大師 讓科學普及不再是夢想〉介紹在科技部資助下已辦理17年的「週日閱讀科學大師」活動。〈讓思考可見－探究式教學遊戲化策略〉介紹強調如何去形塑和看到學生的思考歷程、激發學生學習動機及擴展學生思考界線的探究式教學之遊戲化策略。〈「全國大專暨高中青年物理辯論競賽」真的只是物理辯論?〉則介紹每年五月舉辦的辯論競賽如何進行。 總召編輯委員 - 李隆盛 關於本刊 出版單位：國立臺灣科學教育館 發行人：陳雪玉 總召集人：李隆盛 編輯委員： 物理科吳仲卿/陳耀榮/李柏翰/盧玉玲 | 化學科古建國/許良榮/王伯昌/林如章/周金城 | 生物科王美芬/蕭世輝/陳建志/郭淑妙 | 地球科學許民陽/王郁軒/李文禮/謝隆欽 科技科張玉山/汪殿杰/林育沖/趙珩宇 | 數學科李源順/鄧家駿/溫世展/張宮明 | 跨領域學科李名揚/連信仲 | 特約專欄 游森棚/黃琴扉/陳正改/劉淑雯 策劃：曾聰邦 主編：吳中益 本月專題特約主編：李源順 編輯：陳敬予/張宇茜 網頁設計編輯：施曉恬/張宇茜 投稿規範請來信詢問：article@mail.ntsec.gov.tw

Earthquakes are results of rapid sliding of two crystal blocks around fault inducing seismic motions. Huge disasters caused by large earthquakes as the Ji-Ji Earthquakes occurred on 21* September 1999 show importance of earthquakes forecast. In our country and foreign countries , there appeared not a small number of reports suggesting there are clear precursors of ground water and electro- magnetic changes amount other numerous phenomena. Recently there are reports that point out the possibilities to investigate slight motion of ground water near active faults using electro- magnetic instruments. In order to investigate the problem more in detail a simple laboratory experiment is designed to study the electric field induced by the simulated water flow. The electric field changes are compared with at without water flow. It is shown that there appeared electric potential changes of an order larger than measurement limits water motion during earthquakes occurrence process, and that monitoring the flow of ground water near active faults is useful method in the earthquake prediction.近年來科學家從事地震研究，發現地震前斷層附近地下水流以及電磁場會發生變化。但或因位置不確定，或因變化量甚小而不易量測。本研究真對上述地震預警中兩項重要的關鍵 - 水及電，嘗試製作電極，並利用室內模擬地下水流動而野外斷層的電位量測，檢測斷層活動期間電場的變化。其結果顯示，當地下水因地殼受擠壓而流動時，可明顯觀察到電場的變化。這表示借由本實驗測量地震前發生的電位變化，可監測地震前版塊應力的改變，進而提供地震可能發生的資訊。

We report an investigation on the synthesis, characterization, and application of photonic crystal. In the study of the synthesis of SiO? nanoparticles for the building blocks of photonic crystal, it is found that by changing the concentration of NH3 solution, we are able to control the size of SiO? nanoparticles. After trying several different methods, we discover that the vertical substrate method is the best way to arrange nanoparticles into a periodic structure. From scanning electron microscope, we confirm that SiO? nanoparticles can form a three dimensional hexagonal photonic crystal. From transmission experiment, we find that the wavelength of the minimum transmission is proportional to the size of nanoparticles. This result implies that using photonic crystals we can control the behavior of electromagnetic wave. Finally, we fabricate CdS nanoparticles on the top of photonic crystals with different diameter of SiO? nanoparticles. Using photoluminescence measurements, we show that by controlling the lattice constant of a photonic crystal the luminescent efficiency of CdS nanoparticles can be substantially enhanced. Out results, therefore demonstrate that photonic crystals are very important for the application of light emitting devices. 本研究主要是著重於探討光子晶體合成、特性分析及其應用。在有關合成光子晶體之奈米二氧化矽顆粒方面， 發現在合成過程中利用氨水的溶量可以控制顆粒的大小。在將奈米顆粒排列成光子晶體的研究中， 嘗試了多種方法後， 發現垂直基座法為最快速有效的方法。從掃瞄電子顯微鏡的觀測， 證實奈米顆粒是以六角對稱排列成整齊的光子晶體。在光子晶體的特性分析中， 利用光穿透實驗， 發現電磁波穿透率最小的波長與奈米顆粒成正比關係， 這顯示出可以利用光子晶體來控制光的行為。最後，本研究將光子晶體與硫化鎘奈米顆粒結合，經由光激螢光譜， 證明光子晶體確實可以增進物體之發光效率,這對發光元件的應用， 將有很大的幫助， 可以節省大量的能源