The purpose of this experiment is to determine whether or not wood can be used as an alternative testing method for sunscreens and which species of wood are good for this objective. This will be accomplished by applying varying strengths, or SPFs, of sunscreens onto thin wood veneers. They will then be exposed using a weatherometer, in which real light and sun conditions will be simulated. Using a device called a Pulmac, the samples of wood will be pulled apart from the centre at zero span. Based on the consistency of the strengths, it can be determined how the wood reacts to the sunscreen and whether that species of wood is suitable as a testing medium. Trees are an important commodity which enrich the lives of many people, particularly those who live in British Columbia. They provide the means of living and recreation for numerous people, whether it is employment, business, housing, or camping. Since trees are a renewable resource, they are convenient and realistic to use for testing. There are two possible extensions to this project. If the active ingredients in sunscreens can be determined, then we may be able to use these elements to create a new type of stain for fences and other structures. This could make the necessity for painting less frequent, creating cheaper options for the homeowner. The second possible extension of this project is to use the results to as an initiative to reduce the amount of animal and human testing done by manufacturers. This is important, as animal testing not only causes unnecessary pain, but often the results are not very accurate. Overall, I determined that this method of testing sunscreens would work quite well, especially with the following species, listed in decreasing order. 1) Yellow Cedar 2) Yellow Poplar 3) Maple 4) Hemlock These results were based on how tightly grouped together the means of the strength values were for each species. The closer they were, the more consistent that type of wood and therefore the better they are for testing. Overall, I think this method of testing sunscreens would work quite well, particularly with the yellow cedar, maple, yellow poplar and hemlock, as they resulted in fairly consistent strength values. Generally, the sunscreens with a high SPF, or Sun Protection Factor, resulted in a lower strength loss than the sunscreen with SPF 15. In addition to proving that sunscreen is a good method for preventing UV rays from reaching the skin, this experiment has also confirmed that there are other methods of testing sunscreens, which can be researched further. Along with my main presentation I will be showing various graphs, statistics, and pictures. They will be supplemented by a booklet of “Commonly Asked Questions and Answers” and species descriptions for each type of wood.

本研究（超音波霧化降溫研究）由二實驗組成。實驗一主要針對超音波霧化器（以下簡稱霧化器）之造霧性能進行探討；實驗二則為霧化器之降溫應用。實驗一以改良傳統造霧方式，進而維持最佳造霧效果為主。由於先前的霧化方式是直接放置霧化器於水面，致使最佳霧化水深因霧氣蒸散、水位下降而無法維持；所以在實驗一裡，我們針對霧化器的使用設計一套「漂浮造霧法」：本法運用浮體使霧化器懸於水面，和水面等起伏，使霧化器底部距水面高度不變，藉以維持最佳造霧水深、造霧效果。實驗二乃霧化降溫之探討。本實驗在相同的霧化量下，操縱風速和接觸表面積的差異；利用霧、氣接觸面積與蒸散速率呈正向關係的原理，找出最佳的降溫條件。同時，也期待在兼顧環保的前提下，將之應用於未來開放空間的降溫。The research（Heat Control by Supersonic Vaporization）includes two experiments. One is focused on atomized effect of Supersonic Vaporization（so-called Atomizer）; the other is about applying atomizer to temperature decrease. Exp.1 adapts formal way of atomization. Since previous way of atomization is to put atomizer directly on the water, making the change of atomizer’s distance from water as water evaporates, Exp.1 creates a way called “Floating”. In Floating, atomizer is suspended in a float; constantly keeps the bottom of atomizer from same distance from surface of water. Thus, no matter how much volume of water is evaporated, the best depth of water for atomization and also, the best atomized condition, could stay. Exp2 inquires in relation between atomization and temperature decrease. In Exp2, the atomized volume is fixed, while wind speed and air-contact area are mastered elements. By the theory that, “the wider contact area is, the more efficiently water evaporates,” we could manage out the best condition in temperature decrease. In that way, with the theory practiced, this research, considering environmental case, is supposed to be applied to open-air area one day.

本研究報告以Brocard Point 為核心，所用到的性質均先證明，以確認其正確性，並推演出一些其他的性質，藉由這些性質導出幾何不等式。內容可概分為四部份：(1)以Brocard Angle 及已知的或推演出的基本性質，導出一些不等式。(2)結合「法格乃諾問題」、「費馬點」、「尤拉公式」導出幾個幾何不等式。尤其是三角形邊長與面積，外接、內切圓半徑與邊長間的不等關係，頗為有趣。(3)以向量為工具，分別計算內、重、垂心與Brocard Point 間的距離，並導出邊長的不等關係。其中由內心及重心所導出的不等式，清楚俐落；垂心所導出的不等式則較為複雜。(4)以Brocard Cirle 與內、重心間的關係，導出一系列的不等式。其中Weitgenberk 不等式的無意發現，令我們印象深刻。The Discovery of Geometry Inequalities by Brocard Point This paper takes Brocard Point as a core. We proved some properties about Brocard geometry to confirm its accuracy, and deduce some other properties, and then derive some geometry inequalities by these properties. The content may divide into four parts: a) Derives geometry inequality by Brocard Angle, Crux Mathematicorum and properties which known or deduced. b) Unifies "Fagnano problem", "Fermat Point", "Euler formula" to derive several geometry inequalities. In particular the inequalities between triangle area and length of side, or circumradius inradius and the length of side, is quite interesting. c) Derives geometry inequalities about length of sides in triangle by the distances between incenter centroid circumcenter and Brocard Point. Especially, these inequalities were elegant which derived by incenter and centroid, but it was complicated derived by orthocenter. d) According to the relation about incenter centroid and Brocard Circle derives a series of inequalities. Discover Weitgenberk inequality makes us excited.

此研究是探討光合細菌對污染水中油脂的影響。利用光合細菌(Photosynthesis Bacteria; PSB)來探討對油脂分解的能力，實驗使用的油脂4種，分別是柴油、沙拉油、橄欖油和豬油4種油脂來做為實驗對象。然後取4種不同的油脂各2克分別加入光合菌液98公克做為實驗組，對照組則取4種不同的油脂各2克分別加入海水98公克作為空白對照（不另添加光合菌），每組皆為2重覆實驗，以純光合菌液98公克作為校正組，再利用索氏萃取裝置分離光合菌和水中的油脂，進行油脂回收，實驗進行4週，計算油脂的減少量和油脂分解率作為評估光合細菌對4種不同油脂分解的能力。

本研究是在探討收割後的綠竹筍(Bambusa oldhamii, green bamboo)的老化(aging)現象。一般人說的竹筍老了，通常是指竹筍的質地變硬，口感變差，此即是竹筍硬化的現象，而硬化的主因可能是竹筍受到逆境 (stress) 的刺激後，影響了基因表現的形態，導致纖維素和木質素的增加。竹筍採收後以不同方式處理，觀察切面的變化後發現，以0.2 M蔗糖水浸泡48小時後的竹筍，其切面比浸泡於水中或置於空氣中的竹筍切面較白，筍尖較綠且沒有枯萎的情況。不管是浸泡糖水、水或置於空氣中，都無法防止竹筍的硬化，但浸水和糖水可延緩竹筍硬化的情形，可見要防止竹筍老化，基本上要從抑制合成纖維素與木質素的酵素來著手。抽取竹筍切面處組織中的DNA並以DNA電泳分析之後發現，竹筍的DNA有被降解成小片段的現象，其大小差不多是180 bp的倍數，可見竹筍遇到逆境時也可能會有類似PCD (programmed cell death, 細胞程序性死亡) 的現象。抽取不同處理竹筍的蛋白質進行2D電泳，比較電泳結果發現，三種處理的竹筍的共同點在於減少的蛋白質幾乎都分布在等電點較低的部分。增加的蛋白質大多數分布在等電點較高的區域，這些增加的蛋白質可能和竹筍老現象與PCD有關。本研究還有兩個方向可以繼續延伸研究，第一個是將2D電泳上有明顯差異的蛋白質色點挖出，進行蛋白質定序，再從資料庫中比對，推測可能是何種蛋白質。第二個是研究抑制竹筍合成纖維素和木質素的?的方法，保持竹筍的口感，使竹筍能成為一種能外銷的食品。 The purpose of this study is to investigate and analyze the aging of the harvested green bamboo shoots. The research focused on how to prevent the aging of bamboo shoots and why green bamboo shoots become aging. The term “aging” means that the taste of bamboo shoots becomes hardness post harvest. At first, we tried to find out an anti-aging method, which is not only to keep the green bamboo shoots fresh, but also delicious. The method was to soak bamboo shoots in 0.2 M sucrose, in the water or without any treatment. After 48 hours, the cutting surfaces of bamboo shoots treated with sucrose were whiter, and their outer sheaths were greener than those of shoots soaked in the water or without treatment. The results showed that none of them can stop hardness. But the aging of sucrose- or water-treated shoots was retarded. The results suggest that inhibition of the enzyme activities involving cellulose and lignin synthesis may be required to prevent the aging of bamboo shoot post harvest. To get insight into the reason why bamboo shoots become hardness, the differences of protein patterns and DNA patterns between aging and fresh green bamboo shoots were analyzed and compared. The DNA from the bamboo tissues near cutting surface was isolated and analyzed by agarose gel electrophoresis. The results showed that DNA from shoots was partially degraded. The fragments appear as a ladder of DNA with sizes in multiples of approximately 180 bp. The presence of the olignucleosome-size DNA fragments suggest that the cells may undergo programmed cell death (PCD). The degradation of DNA was not observed in shoots treated with sucrose. By comparing the results of 2-D gel electrophoresis, it was found that some proteins with low pI values decreased or disappeared post harvest, while proteins with increased levels were detected in the high pI area. These changes in these proteins may result in the aging of the bamboo shoots. Prevention of the aging of green bamboo shoots is not easy. However, I found out from this study that soaking the bamboo shoots in the 0.2 M sucrose was a possible way to preserve them. The cutting surface of sucrose-treated shoots remained white, and the sheaths of the shoots was greener than those treated with other methods. Moreover, the degradation of DNA was not observed. However, it still cannot completely stop the aging of bamboo shoots. Reducing the enzyme activities involving cellulose and lignin synthesis may be a direct way to prevent the aging of bamboo shoots. It seems like there are many things to discover in the future.

Astrocytes provide energy to neuron mainly with lactate, which is transported through monocarboxylate transporters (MCTs). Among 14 isoforms of MCTs reported in mammals, only MCT1, 2 and 4 are expressed in brain and the three isoforms are found to differentially expressed in neuron and astrocyte, respectively. Based on these, “astrocyte-neuron lactate shuttle” has been proposed. However, no in vivo evidence was available so far to support this hypothesis. In the present study, zebrafish was used as a model to provide in vivo molecular physiological evidence for the involvements of MCTs in the development and functioning of central nervous system (CNS). Full-length cDNAs of the zebrafish MCT1-4 were cloned from zebrafish. Based on RT-PCR results, zMCT1 and zGLUT4 expressed in brain were chosen for further experiment. Morpholino knockdown experiments provided for the 1st time the in vivo evidence to indicate that the zMCT1 and zMCT4 may be involved in energy translocation and functioning of the developing CNS. 於前人預備實驗中，發現斑馬魚胚胎時期肝醣訊號會於腦部與中樞神經系統區位出現。因此，醣類的分布與在細胞間的傳遞應可視為腦部發育的重要指標。我們以基因體資料庫為工具，研究斑馬魚腦部相關基因異構型分布。目前，我們選殖到數個葡萄糖運輸 (GLUT)、鈉離子/葡萄糖協同運輸(SGLT)與單羧基運輸(MCT)通道在斑馬魚腦部表現，其表現是否與胚胎初期中樞神經系統的發育相關仍未知。經過初步基因分析後，我們決定以MCT 中第一和四異構型作為研究對象，並用Anti-Hu，與PCNA 做神經觀測，將施打反股寡核?酸進行突變操作的斑馬魚，與對照組進行免疫染色分析，以螢光顯微鏡觀察發現：抑制MCT 表現，確實影響胚胎時期中樞神經細胞發育及鰓部細胞分裂；亦可能造成畸形出現。

本研究主要的目的是在開發二氧化碳製造、過濾與循環的整合型水族養殖系統。二氧化碳的產製是以基於發酵原理，並搭配具有純化二氧化碳作用的水濾裝置，發酵環境溫度以水族箱內的水來維持，循環過程中並搭配外部過濾器，所有容器均由保特瓶改裝完成，整合成一套價廉、環保且具有高效率的水族養殖系統。由實驗結果可得知，二砂糖發酵對於二氧化碳產生率而言，其最佳發酵環境溫度範圍25℃~35℃，一般水族箱的水溫即可保持在此一溫度範圍。過濾系統則使用光電比色計與一般市售過濾器相比較，亦有令人滿意的效果。本實驗除了研究以二砂糖發酵之外，還希望更進一步地朝向廚餘減量方面開發其他發酵材料，希望能為環保盡一份心力。;The purpose of this study is to develop an integrated system of aquatic breeding with the functions of producing carbon dioxide, filtering and circulating, where carbon dioxide is produced based on fermentative theorem and purified by the water filtering; the environmental temperature of fermentation is maintained by the water in the aquarium and circulating is through an external filter. All the containers are made of PET bottles and thus integrated a cheap, environmentally friendly and high efficient system of aquatic breeding. According to the experimental result, for the producing rate of carbon dioxide, the best fermentative temperature of NO.2 granulated sugar is 25℃~35℃. With a photocell colorimeter to compare the function of the self-made filter with that of the filters on the market, one can find it’s comparable. In addition to the fermentation of NO.2 granulated sugar, the study is also expected to develop other fermentative materials to reduce kitchen waste for environmental protection.

中文摘要：\r 化學實驗中，常常會使用到各種具有揮發性的有機溶劑，如醇類、酸類、醛類及酮類等。\r 當吸入過量的揮發性有機溶劑時，將會對人體造成嚴重的傷害，故使用時格外需要留意。有\r 鑑於此，我們開始著手研究如何偵測氣相中的有機氣體分子。\r 本研究中，我們利用聚苯胺薄膜吸附氣體分子的特性並結合對質量變化極靈敏的石英晶\r 體微天平，自行設計了一套簡單、藥品用量少且不需昂貴儀器的實驗系統。藉由此系統，我\r 們有效的針對各種揮發性有機溶劑進行偵測，並進一步地探討不同氣體分子對於聚苯胺薄膜\r 的吸附現象。\r \r 英文摘要：\r Students usually use volatile organic solvent in laboratorys such as alcohols, carboxylic acids,\r aldehydes, ketones, etc. It will injure our body when we breathe in too much volatile organic\r solvent. For this purpose, we begin to study how to detect volatile organic compound in air.\r In this work, we combine polyaniline membrane which can adsorb gas molecules and quartz\r crystal microbalance which is highly sensitive to mass changes to design a simple and inexpensive\r system which needs only little chemicals. With this system, we can effectively detect different\r volatile organic compounds. Furthermore, we can also study the adsorption of polyaniline\r membrance for different volatile organic compounds.

臉部皮膚會長出小小骨頭喔！皮膚多發性粟粒型骨瘤的發現，X光顯像，治病機轉與雷射在治療上的應用。本篇研究是探討一種特殊又不易偵測的骨瘤，皮膚多發性粟粒型骨瘤(MMOC)，利用各種X光顯像技術，應用在痤瘡病患，偵測其分佈、發生率和疾病之可能相關；並利用組織病理發現去推測致病機轉；且利用各種雷射光，在體內與體外進行治療性的探索，並獲致重要的結論如下：(1)MMOC非常常見，每個人都有可能發生，但臨床不易被偵測，跟性別無關，但與年齡增長成正相關；(2)病灶分佈包括額頭、眼皮、鼻子、臉頰及頸部，與痤瘡的病灶分佈十分類似，但與痤瘡的疾病活性與疤痕的嚴重度並無相關。(3)利用乳房攝影的斜切照影技術，是最簡易有效的偵測方式。(4)病理組織中有些皮膚腫瘤伴有MMOC存在，並非此腫瘤有其特異性。(5)MMOC是一個因為皮膚毛囊皮脂腺在不明顯的發炎下所造成的轉化型骨形成反應。(6)紅寶石雷射及銣-雅鉻雷射對色素性骨瘤是一有效的去除技術。鉺-亞鉻雷射及二氧化碳雷射磨皮技術，對嚴重痤瘡疤痕並有MMOC患者，亦為一良好治療方式。Multiple Miliary Osteoma Cutis(MMOC) is a kind of special condition and difficult to detect clinically. By using different kinds of X-ray techniques and compared the clinical data from the patients with acne of face, by observing the changes of skin tissue specimens for searching the possible mechanism of osteogenesis and by using scanning electron microscopic observation and energy dispersive Xray analysis for evaluating the biophysical effects on osteoma after Ruby, ND-YAG, Erbium:YAG or CO2 Laser irradiation, we concluded that: MMOC, a very common condition, may develop in everyone. MMOC can be easily demonstrated by the modified mammographic technique. The density of calcification in MMOC is not related to the sex of patients, the activity of acne, and the severity of atrophic scar but correlated with the age of the patients. MMOC, a non-specific condition, results from metaplastic ossification occurring within preexisting subclinical inflammatory processes of the pilosebaceous unit and may be found incidentally in different kinds of facial tumor. Ruby or ND-YAG laser may have some explosive effect on pigmented MMOC. CO2 or Erbium:YAG laser dermabrasion can be used to treat MMOC with acne scar.

A vast amount of research has been conducted on various ant species such as Monomonum pharaonis, Formica rufa, and Lasius niger, demonstrating the coexistence of polygyny and monogyny colonies. In polygyny colony, the high heredity variability of filial generation could be expected, but what could the monogyny colony do to hold the high heredity variability in order to increase the fitness? I hope I can account the sex ratio in the colony of spiny weaver ants, Polyrhachis dives, and compare the different colony with different reproductive strategies. I also want to check by V8 that the colony can form a polygyny colony and virgin queens can mate with many males of the same colony in the artificial nest. The results suggest that in the colony of spiny weaver ants, the higher the number of the dealate queens, the lower the number of the male. In the monogyny colony, workers control the sex ratio. According to the observation, we can see the alate adult mate in the artificial nest, and multimating can be recorded for the first time. The following study was there for undertaken to examine what’s the aftereffect made by the Reproductive strategies of polygyny colony and monogyny colony.在多后聚落內，子代變異度高是可以預期的，但是單后聚落中，又如何維持遺傳的變異度以增加其對環境的適應力呢？我們希望能計算黑棘蟻聚落組成，比較不同生殖策略的聚落之各種性比，並且錄影證實黑棘蟻聚落可以為多后聚落並進行巢內自交。我們發現：黑棘蟻聚落內，蟻后數愈高，則雄蟻比例較低；單后聚落之性比決定於工蟻；而根據錄影觀察，聚落中的有翅生殖族可在人工蟻巢中完成交配，多次交配的現象首次被實地紀錄，並且佔所有交配新生蟻后的92.8%。本實驗可利用黑棘蟻來了解單后與多后聚落的生殖策略對其族群發展的影響。

This research is about two ponds in the Behu park’s and the Dahu park’s snail(Square Mystery Snail：Sinotaia quadrata) in Taipei city of Nei-hu District for research object, carry out the study of the following research proceed: 1.Discriminate the algae species that are growth on the snail shell and which is a kind of interaction with the snail; 2.The influence of the snail and algae with difference of temperature, salinity, pH value and dark ; 3. The factors affect algae growth on snail shell; 4.Use the variation of snail and algae to be a biological incator. The result manifestation: the algae that are growth on snail shell have two kinds, one is Oscillatoriaceae and the other is Cladophora sp. The interaction between algae and snail belong to communalism, but under the condition of lacking of food, the snail then will eat the Cladophora sp. which grow on the shell of other snails. The temperature adapts aspect, upper limit of the feat existence of the snail should be low in 28℃. When over than 28℃, Cladophora sp. as the most strong, Oscillatoriaceae is secondly, and the snail then is most poor. For the maximum tolerance of the salinity, the snail is about 4.38?, Oscillatoriaceae is about 5.00?, Cladophora sp. is then about 5.83?; Under the different salinity for the tolerance , the Cladophora sp. still the most strong, Oscillatoriaceae is secondly, and the snail then is most poor. Under the dark environment, the speed of Oscillatoriaceae begin to be bleaching is very fast than the Cladophora sp.. In the tolerance of pH value range: The snail is about pH=5～10, Oscillatoriaceae is about pH=7～8, Cladophora sp. is about pH=6～8; When the pH value range is in the pH=5～8, the speed of the Oscillatoriaceae occur changing is very fast than Cladophora sp.. The algae are growing on snail shell very different between two ponds, the main reason is water pH value dissimilarly: When pH value over than 8.5, there is no Cladophora sp. to grow on the snail shell, after the pH value to decrease, Oscillatoriaceae then will compare early than Cladophora sp. to grow on the snail shell. Calculate by the classification of the freshwater biological incator : Two organic pollution degree of the ponds may be lain in theβ-mesosaprobic to theα-mesosaprobic, and the polluting degree of the Dahu pond is more seriously. As for two ponds, have already faced what level of eutrophication? Belong to actually which stage of pollution grade? Not only added the classification data of floating and fixative algea in two ponds, and also according to the parts of chemistry analysis method measure of the data makes the substantial evidence, then could carry out the more accurate and thorough study in the days to come steadily studying process.本研究是以臺北市內湖區兩個綠地公園(碧湖公園與大湖公園)池塘內的石田螺(Sinotaia quadrata)為研究對象，進行以下研究目的之探討：1.鑑別石田螺螺殼上藻類的種類及其與石田螺的互動關係；2.溫度、鹽度、酸鹼值及黑暗等環境因子的差異，對石田螺及螺殼上附生藻類的影響；3.影響藻類附生於石田螺螺殼上的因素；4.將石田螺及螺殼上附生藻類的變化作為監測環境因子或水質變異的指標現象。結果顯示：附生於石田螺螺殼上的藻類有顫藻(Oscillatoriaceae)與剛毛藻(Cladophora sp.)兩類；與石田螺的互動關係應屬於片利共生(communalism)，但在缺乏食物的情況下，石田螺則會採食同伴殼上的剛毛藻。溫度適應方面，石田螺適宜生存的溫度上限應低於28℃，超過28℃水溫環境的耐受程度，是以剛毛藻為最強，其次是顫藻，而石田螺則為最差。對於環境鹽度最大耐受度方面：石田螺約為4.38?，顫藻約為5.00?，剛毛藻則約為5.83?；在不同鹽度環境下，鹽度的耐受程度，仍以剛毛藻為最強，其次是顫藻，而石田螺則是最差。在黑暗環境下，顫藻褪色產生白化現象的速度明顯地比剛毛藻要快了許多。在環境酸鹼值耐受的範圍方面：石田螺約在pH＝5～10 之間，顫藻約在pH＝7～8 之間，剛毛藻則約在pH＝6～8之間；而酸鹼值範圍在ｐＨ＝5～8 時，顫藻產生變化的速度明顯地比剛毛藻還要快。而兩樣區池塘水體酸鹼值的不同，應是造成石田螺螺殼藻類附生現象差異的主要原因：當酸鹼值超過8.5 時，螺殼上就無剛毛藻附生，當酸鹼值降下後，顫藻則會比剛毛藻早出現在螺殼上。藉由淡水生物指標的分類推測：兩樣區池塘水體有機污染程度，可能介於β-中腐水性(β-mesosaprobic,βm)至α-中腐水性(α-mesosaprobic,αm)的範圍之間，而Ｄ池塘受污染的程度應會比Ｂ池塘還要更嚴重些。至於兩樣區池塘水體，已面臨了何種優養化的程度？究竟是屬於哪一個階段的污染等級呢？除須補充水體中浮游性及附著性藻類分類的詳細觀察資料外，仍必須參照部分水質化學分析法所測得的數據作佐證，才能在日後持續地研究過程中進行更精確及深入的探討。

A pair of bacterial two-component system RssB-RssA was cooperated into Serratia marcescens for toxicity phenolic compound detection. First step of this study, E coli was used to accept the plasmid and certified by fluorescent. Then transfer the system from E coli into Serratia marcescens. Finally, 7 kinds of chemical, included phenol, benzene, toluene, xylenes, 4-chlorotoluene, 2-nitrotoluene, and kerosene, were used to check the sensitivity of this gene modified Serratia marcescens line. The results showed that this gene modified Serratia marcescens line had good performances and responses to those chemicals. 本實驗是以一受到二元訊號傳遞系統調控的發光基因重組質體，送入黏質色拉雷菌中，並以製備好的菌株進行毒性化合物之測試。在實驗的第一階段，我們將重組質體送入大腸桿菌內，並以其發光的有無來判斷是否達到送入的目的，其後再以電泳法確認各基因片段是否正確。第二階段再以相同的方法將選殖好的重組發光質體送入黏質色拉雷菌。第三階段，以發光重組菌針對酚、苯、甲苯、二甲苯、4-氯甲苯、2-硝基甲苯及煤油進行發光測試。結果方面，我們發現黏質色拉雷菌發光重組菌對於這一系列的酚環類化合物的確具有相當高的敏感度。