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溪水清清-溶膠凝膠法製備二氧化鈦奈米粒子及其在環境保護應用研究

中文摘要:\r 本文利用溶膠-凝膠法(Sol-Gel method)製備二氧化鈦奈米粒子(Anatase\r 晶相),測定廷得耳效應判斷其是否為奈米粒子,並以二氧化鈦奈米粒子加入水\r 中,以紫外光照射測試其光催化分解水反應的活性,並測試其分解南崁溪河川廢\r 水的能力。結果顯示自製二氧化鈦奈米粒子具有明顯的廷得耳效應,光催化分解\r 水反應因紫外光源功率過低未能呈現,有待改進;分解河川廢水的能力仍在進行\r 中。\r Abstract\r This experiment produces Titanium dioxide nano-particles (Anatase crystal\r phase)by using Sol-Gel method. We prove the particles as nano-order by the Tyndall\r effect And adds Titanium Dioxide into water, illuminate it with special wavelength\r ultraviolet radiation. The activity of its light catalysis reaction to water was tested.\r The capability of the treatment of wastewater in Nancan River by nano-titanium\r particles was also studied. The results show that the powder prepared by this\r experiment has apparently Tyndall effect,the photo-catalysis reaction of water is\r failed due to the weak power of UV light sources. The test of wastewater treatment is\r still processed.

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形?與形外

在這篇研究報告中,我用了三種觀點來推廣幾何中的反演變換,首先,把反演變換視為是一種圓內與圓外的一種1-1且onto的映射,第一種推廣,是將變換中心移到視圓心以外的圓內的地方,馬上我們得到一個結論「反演半徑會隨著動點而改變」,接著,我們實驗了一下反演變換用有的一些性質,保角性,保圓性,…等在這個變換視中是否依然存在;接著我們用第二種方法來推廣反演變換,我們將邊界的形狀由圓視改成別的形狀(如三角形,四邊形…等等),然後也試試看在這種變換之下是否還擁視有反演變換的一些性質;第三種推廣,則是在研究的過程中,我發現了一種新的幾視變換,承接第一種推廣,我們將原先為定點的變換中心改為動點,將原先的動點改為定點,做出來的一種新變換。In the study, a new geometric Inversive transformation through three points is discovered. Here is the main result:(1)The first, onto cycle of inside and outside can be proved under invasive transformation. It is changed moving the center from center of cycle, we can get a new ” Inversive radius can be changed by moving drop. (2) We hope to find the answer to this problem by experiment, it is exist with the inversive properties. (3) A new geometric transformation is discovered, a fixed drop can be changed moving drop, then the first moving drop shifted the fixed drop. This leads to a new construction if the new transformation.

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竹筍老化之謎

本研究是在探討收割後的綠竹筍(Bambusa oldhamii, green bamboo)的老化(aging)現象。一般人說的竹筍老了,通常是指竹筍的質地變硬,口感變差,此即是竹筍硬化的現象,而硬化的主因可能是竹筍受到逆境 (stress) 的刺激後,影響了基因表現的形態,導致纖維素和木質素的增加。竹筍採收後以不同方式處理,觀察切面的變化後發現,以0.2 M蔗糖水浸泡48小時後的竹筍,其切面比浸泡於水中或置於空氣中的竹筍切面較白,筍尖較綠且沒有枯萎的情況。不管是浸泡糖水、水或置於空氣中,都無法防止竹筍的硬化,但浸水和糖水可延緩竹筍硬化的情形,可見要防止竹筍老化,基本上要從抑制合成纖維素與木質素的酵素來著手。抽取竹筍切面處組織中的DNA並以DNA電泳分析之後發現,竹筍的DNA有被降解成小片段的現象,其大小差不多是180 bp的倍數,可見竹筍遇到逆境時也可能會有類似PCD (programmed cell death, 細胞程序性死亡) 的現象。抽取不同處理竹筍的蛋白質進行2D電泳,比較電泳結果發現,三種處理的竹筍的共同點在於減少的蛋白質幾乎都分布在等電點較低的部分。增加的蛋白質大多數分布在等電點較高的區域,這些增加的蛋白質可能和竹筍老現象與PCD有關。本研究還有兩個方向可以繼續延伸研究,第一個是將2D電泳上有明顯差異的蛋白質色點挖出,進行蛋白質定序,再從資料庫中比對,推測可能是何種蛋白質。第二個是研究抑制竹筍合成纖維素和木質素的?的方法,保持竹筍的口感,使竹筍能成為一種能外銷的食品。 The purpose of this study is to investigate and analyze the aging of the harvested green bamboo shoots. The research focused on how to prevent the aging of bamboo shoots and why green bamboo shoots become aging. The term “aging” means that the taste of bamboo shoots becomes hardness post harvest. At first, we tried to find out an anti-aging method, which is not only to keep the green bamboo shoots fresh, but also delicious. The method was to soak bamboo shoots in 0.2 M sucrose, in the water or without any treatment. After 48 hours, the cutting surfaces of bamboo shoots treated with sucrose were whiter, and their outer sheaths were greener than those of shoots soaked in the water or without treatment. The results showed that none of them can stop hardness. But the aging of sucrose- or water-treated shoots was retarded. The results suggest that inhibition of the enzyme activities involving cellulose and lignin synthesis may be required to prevent the aging of bamboo shoot post harvest. To get insight into the reason why bamboo shoots become hardness, the differences of protein patterns and DNA patterns between aging and fresh green bamboo shoots were analyzed and compared. The DNA from the bamboo tissues near cutting surface was isolated and analyzed by agarose gel electrophoresis. The results showed that DNA from shoots was partially degraded. The fragments appear as a ladder of DNA with sizes in multiples of approximately 180 bp. The presence of the olignucleosome-size DNA fragments suggest that the cells may undergo programmed cell death (PCD). The degradation of DNA was not observed in shoots treated with sucrose. By comparing the results of 2-D gel electrophoresis, it was found that some proteins with low pI values decreased or disappeared post harvest, while proteins with increased levels were detected in the high pI area. These changes in these proteins may result in the aging of the bamboo shoots. Prevention of the aging of green bamboo shoots is not easy. However, I found out from this study that soaking the bamboo shoots in the 0.2 M sucrose was a possible way to preserve them. The cutting surface of sucrose-treated shoots remained white, and the sheaths of the shoots was greener than those treated with other methods. Moreover, the degradation of DNA was not observed. However, it still cannot completely stop the aging of bamboo shoots. Reducing the enzyme activities involving cellulose and lignin synthesis may be a direct way to prevent the aging of bamboo shoots. It seems like there are many things to discover in the future.

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「嘴」大「顎」極,飢要擇食-水蠆覓食策略探討

本實驗主要目的是以行為生態學的觀點來探討烏點晏蜓(Anax guttatus)水蠆的攻擊發動之條件,以及面對獵物採取的捕食策略。本實驗結果發現,水蠆發動攻擊的起因與獵物的大小及距離均呈負相關。而發動攻擊的範圍多在下唇顎所及之處,支持水蠆為伏擊型獵食者(sit andwaiting predator)。面對不同大小獵物時會有所選擇,因為極小或極大的獵物難以捕食,且在捕獵大獵物會消耗較多時間成本,所以水蠆會在獵食風險(失敗、受傷、時間成本)及獲益(能量)之間取得平衡。當水蠆面對獵物密度高低不同的環境,會因水蠆體型大小出現積極、保守、消極的不同策略。綜合以上實驗結果,水蠆在捕食行為上能做最適的權衡(trade off),選擇以最低的風險與成本獲得最高能量,為生態中最佳捕食者之一。

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別鬧了,辛普森先生

We investigate the machinery producing successive Simpson’s paradoxical reverse. Taking advantage of algebraic and geometric techniques, we obtain the following results. Take playing baseball for example. In our study, we find that Simpson’s paradox only occurs when the hitter’s hits over 3 times in one game. Set n equal to the times I will hit in one game. If my batting average in each game is at least(n ?1)/2 times higher than the others’; then I am sure that my total batting average would not be invert by the others. In order to find how many the lattice points in the triangle, we use Pick’s formula. But sometimes, the Pick’s formula is not appropriate to triangles whose vertex are not all lattice points. So we develop New Pick’s formula to estimate the number of lattice points in such kind of triangles. Besides, we also find an iterative algorithm to produce successive “Simpson reverse” phenomenon by using C++ language, and we can therefore produce as many “Simpson’s set of four sequences” terms as we like(not beyond the computers’ upper limit).Moreover, if both sequences of ratios converge, then they must have the same limit.我們探討了一般人乍看之下顯得頗弔詭的辛普森詭論。我們配合GSP 作圖,用解析幾何、設立直角座標系和C++ 程式的運算,找出在特殊情況下或一般情況下所產生的辛普森數列組和特殊的性質,並且以棒球場上的打擊率為例子來做印證。通常一場棒球賽中,每個人平均上場3 次~4 次,經過我們的討論,發現要發生逆轉的機會只有在上場達到4 次或以上時才會發生。?了求出在直角座標系中可以滿足的格子點個數,我們用了Pick公式,但?了更準確的估計,我們引進了虛點的概念,重新推導出了新Pick 公式。另外,我們還發現,假設兩個人上場比賽,若打了2 場,且每場最多上場打擊K 次,其中的一個人的打擊率只要是另一個人的(k-1)/2倍以上就保證不會被逆轉。我們又找到了連續產生辛普森逆轉的演算法,利用C++ 寫出程式,經由演算法和遞迴式,製造出項數可任意多(只要電腦能夠承受)的辛普森數列組,且我們發現若兩個比值數列接收斂,則極限趨近於同一個數值。

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科學研習月刊

59-04

NO.59-04 2020 AUG | 科學研習期刊目錄 本期專題 核心素養與數學素養 數學領域核心素養與數學素養之我見 | 李源順 咖啡豆的數據分析 | 洪士薰 燈謎與數學 | 李祐宗 有數學感的教與學:以比較型問題為例 | 李源順 教學現場 個案小學教師在理財情境中數學素養的專業成長 | 湯永麟 素養導向的數學課室樣貌―以國小「比與比值」的教學為例 | 溫世展 12年國教精神在國小數學課室之實踐樣貌 | 陳玉珊 從數學奠基進教室談素養教學─以「棋奕三視圖」為例 | 林柏寬 科學新知 單淘汰賽中的數學-高中數學的探究與實作 | 張宮明 摺出畢氏數 | 林靖捷 透過摺紙也可以學數學推理 | 譚克平 特約專欄 以建築為主題的跨領域探究學習 | 劉淑雯、陳韻如、褚希雯 炫光手持木鋼琴STEAM創意課程 | 儲述傑、丁于真、黃琴扉 下雨了「梅」? | 江鈞、李竑慶、呂奕勳、林育慎、侯宜妤、林威帆、王嘉琪 森棚教官數學題 全數出動 | 游森棚 科普活動報導 週日閱讀科學大師 讓科學普及不再是夢想 | 李旺龍 讓思考可見-探究式教學遊戲化策略 | 陳佩雯、呂玉環、程詩婷、顏慈瑤、蘇萬生 「全國大專暨高中青年物理辯論競賽」真的只是物理辯論? | 余進忠、蘇萬生 總召集人的話 12年國教課綱強調透過各學習領域的分工與合作培養學生的「核心素養」,在分工上數學學習領域旨在培養學生「數學領域核心素養」和/或「數學素養」。本期特別以「核心素養與數學素養」為專題。 「本期專題」單元共有四篇文章,〈數學領域核心素養與數學素養之我見〉解說「數學領域核心素養」和「數學數養」個別定義與兩者關係。人工智慧很適合用來發展瑕疵咖啡生豆的挑選法,〈咖啡豆的數據分析〉即介紹如何使用簡單工具、運用高中數學中的知識,讓學生動手實作體驗數據分析與人工智慧。 〈燈謎與數學〉分享51則供讀者自學或教學的參考的數學燈謎。〈有數學感的教與學:以比較型問題為例〉介紹如何透過學生的親身經驗等,了解比較型問題的意義,以培養帶得走的能力,以及學習數學的感覺。 「教學現場」單元也刊登四篇文章,〈個案小學教師在理財情境中數學素養的專業成長〉分享一位小學老師在理財情境中數學素養之專業成長的探究經驗。〈素養導向的數學課室樣貌―以國小「比與比值」的教學為例〉分享自行設計的「比和比值」教學活動,結合數學感理論的數學課室實踐。〈12年國教精神在國小數學課室之實踐樣貌〉分享在施教「基準量和比較量」單元時,讓學生透過「同儕間『抓』出『錯』誤之處」的「抓錯」活動經驗。〈從數學奠基進教室談素養教學─以「棋奕三視圖」為例〉則分享以「棋奕三視圖」為例的數學奠基進教室活動經驗。 「科學新知」單元刊登三篇文章,〈單淘汰賽程安排中的數學〉分享在國內及國際獲獎專題研究「單淘汰制賽程分析」中的數學。〈摺出畢氏數〉分享「摺出畢氏數」課程、教具以及「畢氏數書籤」作品。〈透過摺紙也可以學數學推理〉介紹和數學推理有關的四個摺紙基本技巧。 「特約專欄」單元刊登四篇文章,〈跨領域課程發展與教學實踐初探〉分享作者和某國小合作推動跨領域課程發展與教學實踐的理念和作法。〈炫光手持木鋼琴STEAM創意課程〉分享可帶入STEAM實作、實現跨領域的知識統整與 應用的炫光手持木鋼琴教學活動。〈下雨了「梅」?〉探討梅雨季未來的降雨變化趨勢,並傳達氣候變遷下應有的防災資訊。〈森棚教官數學題:全數出動〉拋出1,11,111……分別被同一數值除後的餘數問題。 「科普活動報導」單元刊登三篇文章,〈週日閱讀科學大師 讓科學普及不再是夢想〉介紹在科技部資助下已辦理17年的「週日閱讀科學大師」活動。〈讓思考可見-探究式教學遊戲化策略〉介紹強調如何去形塑和看到學生的思考歷程、激發學生學習動機及擴展學生思考界線的探究式教學之遊戲化策略。〈「全國大專暨高中青年物理辯論競賽」真的只是物理辯論?〉則介紹每年五月舉辦的辯論競賽如何進行。 總召編輯委員 - 李隆盛 關於本刊 出版單位:國立臺灣科學教育館 發行人:陳雪玉 總召集人:李隆盛 編輯委員: 物理科吳仲卿/陳耀榮/李柏翰/盧玉玲 | 化學科古建國/許良榮/王伯昌/林如章/周金城 | 生物科王美芬/蕭世輝/陳建志/郭淑妙 | 地球科學許民陽/王郁軒/李文禮/謝隆欽 科技科張玉山/汪殿杰/林育沖/趙珩宇 | 數學科李源順/鄧家駿/溫世展/張宮明 | 跨領域學科李名揚/連信仲 | 特約專欄 游森棚/黃琴扉/陳正改/劉淑雯 策劃:曾聰邦 主編:吳中益 本月專題特約主編:李源順 編輯:陳敬予/張宇茜 網頁設計編輯:施曉恬/張宇茜 投稿規範請來信詢問:article@mail.ntsec.gov.tw

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本土藥材金銀花的研究與分析

本實驗以薄膜色層層析(TLC)、高效能液相層析(HPLC)分析等化學方法,進行金銀花品種差異的鑑識;此外,配合生藥學的顯微鏡檢視,如中藥材組織鏡檢、藥材粉末鏡檢等比對,以期找出辨別金銀花品種的方法。研究至目前為止,由金銀花之薄膜色層分析的Rf值(0.225、0.425、0.7、0.85、0.95)可確認出金銀花藥材,並得知金銀花藥材中皆含有綠原酸的成分;以高效能液相層析的圖譜與成分峰的積分面積可用來判別金銀花的品種,並從質譜分析瞭解成分含量;進行生藥學的藥材粉末組織鏡檢,發現無法作為金銀花藥材的分類憑藉。未來將持續延伸實驗,朝向中藥奈米化與一般粉末在藥效、成分上差異的比較,並進行金銀花萃取液的抗菌作用試驗,瞭解不同品種之金銀花藥材在藥理作用的異同,接續著奈米化藥材的應用與實踐。Using thin layer chromatograph (TLC) and high performance liquid chromatography (HPLC), we can study how to differentiate the species variation of honeysuckle; beside, based on the observation of biopharmaceutical microscope, such as comparing the histology of Chinese herbs and its powder, we suggest that we could differentiate the species of honeysuckle. From the present, firstly, we could distinguish the honeysuckle from other herbs by the Rf value of TLC(0.225, 0.425, 0.7, 0.85, 0.95), from which we find that all honeysuckles contain the component of Chlorogenic acid. Secondly, we could tell the species of honeysuckle according to the map of HPLC and the peak area after integration, as well as the integrants of honeysuckle by way of LC-Mass analysis. Thirdly, while studying the histological analysis based on the observation of biopharmaceutical microscope, we found that it shows no difference between all the honeysuckles; thus, it fails to be a scientific method used to distinguish the herb honeysuckle. However, in the biochemical experiments of honeysuckles, we found honeysuckles from different sources and the place of origin shows difference in their antibiotic effect, showing the importance of local medicine. When it comes to my future work, in order to extend my experiments on honeysuckles, I would compare the nano-scale honeysuckle powder with normal-sized one in their clinical effects and components.

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光觸媒(TiO2)對還原重金屬離子之研究

光催化氧化反應以半導體金屬氧化物為催化劑,進行有機性空氣污染物之快速分解反應。其原理係將半導體材質(如:二氧化鈦,TiO2),在適合之光能量照射下,將半導體激發成為具有氧化/還原能力之催化劑,可加速氧化還原反應之進行,迅速分解有機污染物。 研究動機在於利用TiO2在紫外光的照射下,將H2O 分解產生自由基,使其和水中的重金屬離子進行氧化還原反應,期待可以還原水中的重金屬藉以降低水中重金屬離子濃度,同時藉由使用界面活性劑對奈米微粒具保護作用,可回收重金屬奈米微粒。 由實驗結果得知在紫外光照射下,TiO2 使用量0.5 克,AgNO3(aq) 0.01M,照光24 小時其電導度值上升最多且在溶液表面觀察到銀白色銀金屬薄膜生成而所測得銀金屬析出量明顯增加。探討超音波振盪對TiO2還原力的影響得知,超音波震盪的時間越久,所上升的電導度值愈多。 探討常見的界面活性劑(陽離子型及陰離子型)對TiO2還原力的影響:從數據中可觀察到,加入陰離子界面劑時,電導度值明顯上升;而加入陽離子界面活性劑後,電導度值迅速下降,照光後電導度值也不理想。 探討日光及不同頻率的紫外光照射光源對TiO2還原力的影響:發現紫外光的波長愈短,銀金屬析出的量愈多。 探討除氧處理之溶液對TiO2 還原力的影響得知,除氧處理後所配製的AgNO3(aq)0.01M, 經照光24 小時後電導度值明顯上升,且在溶液表面觀察到大片銀白色銀金屬薄膜生成而所測得銀的析出量也大幅增加。 ;In the experiment, we used the properties of T i O 2 that can catalyzed by UV rays and breaking the molecules of water and produce free radicals, which free radicals can redox metallic oxide as accelerator to analyze organic pollutant briskly. We use different shinning time and to find the best effect of the redox reaction . So we want to use this attribute to begin the redox reaction with the metal ion (for example: Ag+) in water, expecting to reduce them. And then we can use this method to recycle the metal and to reduce the pollution in rivers. Throughout shining UV rays in 24 hours, we can find out the best effect of TiO2 reducing the metal ion solution. We can also find that we use ultrasonic first; the more redox it will have. In this research ,we can observed that if we put more anion surface-active agent, the more redox it will have. We find the effect of UV rays is better than visible light. The most important is that we deoxidize the metal ion solution, we can get the best effect of the redox reaction . In our research,we can't get the satisfing result of the copper sulphate (CuSO4) by TiO2 accelerating the redox reaction under UV rays.

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摺紙數列-相關問題探討

1. 遊戲規則:將1~ 2m × 2n的連續正整數,由上而下、由左而右依序填入 2m × 2n的方格內。操作規則允許將2m × 2n做往右或往左或往上或往下的完全對摺,直到操作至所有單位方格均疊成一行,此同時有數字也由上而下形成一數列。2. 本研究即是探討操作完成的數列之數量與數字間的關連性。3. 我們發現:(1) 數列之數量與巴斯卡三角形有關。(2) 形成的數列必符合內文的 [ R(L) 性質]、 [ D(U) 性質]、[ R&D 性質]、[D&R 性質]。 1. Rules of thegame: Fill in order the continuous positive integers 1~ 2m × 2n, from top to bottom and from left to right in the 2m × 2n check. The operational rule allows a complete fold of 2m × 2n either rightward or leftward, or upward or downward, until all the check units pile up in a line. At the same time, all the integers form a series from top to bottom. 2. This study explores the relationship between the number of the series and the integers after the operation. 3. Our findings are: (1) The number of the series is related to Pascal triangles. (2) The series formed meet the properties mentioned in the study: [the property of R(L)], [the property of D(U)], [the property of R & D], and [the property of D & R].

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一后多夫否?黑棘蟻(Polyrhachis dives)聚落生殖策略之研究

A vast amount of research has been conducted on various ant species such as Monomonum pharaonis, Formica rufa, and Lasius niger, demonstrating the coexistence of polygyny and monogyny colonies. In polygyny colony, the high heredity variability of filial generation could be expected, but what could the monogyny colony do to hold the high heredity variability in order to increase the fitness? I hope I can account the sex ratio in the colony of spiny weaver ants, Polyrhachis dives, and compare the different colony with different reproductive strategies. I also want to check by V8 that the colony can form a polygyny colony and virgin queens can mate with many males of the same colony in the artificial nest. The results suggest that in the colony of spiny weaver ants, the higher the number of the dealate queens, the lower the number of the male. In the monogyny colony, workers control the sex ratio. According to the observation, we can see the alate adult mate in the artificial nest, and multimating can be recorded for the first time. The following study was there for undertaken to examine what’s the aftereffect made by the Reproductive strategies of polygyny colony and monogyny colony.在多后聚落內,子代變異度高是可以預期的,但是單后聚落中,又如何維持遺傳的變異度以增加其對環境的適應力呢?我們希望能計算黑棘蟻聚落組成,比較不同生殖策略的聚落之各種性比,並且錄影證實黑棘蟻聚落可以為多后聚落並進行巢內自交。我們發現:黑棘蟻聚落內,蟻后數愈高,則雄蟻比例較低;單后聚落之性比決定於工蟻;而根據錄影觀察,聚落中的有翅生殖族可在人工蟻巢中完成交配,多次交配的現象首次被實地紀錄,並且佔所有交配新生蟻后的92.8%。本實驗可利用黑棘蟻來了解單后與多后聚落的生殖策略對其族群發展的影響。

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由Brocard Point 發現幾何不等式

本研究報告以Brocard Point 為核心,所用到的性質均先證明,以確認其正確性,並推演出一些其他的性質,藉由這些性質導出幾何不等式。內容可概分為四部份:(1)以Brocard Angle 及已知的或推演出的基本性質,導出一些不等式。(2)結合「法格乃諾問題」、「費馬點」、「尤拉公式」導出幾個幾何不等式。尤其是三角形邊長與面積,外接、內切圓半徑與邊長間的不等關係,頗為有趣。(3)以向量為工具,分別計算內、重、垂心與Brocard Point 間的距離,並導出邊長的不等關係。其中由內心及重心所導出的不等式,清楚俐落;垂心所導出的不等式則較為複雜。(4)以Brocard Cirle 與內、重心間的關係,導出一系列的不等式。其中Weitgenberk 不等式的無意發現,令我們印象深刻。The Discovery of Geometry Inequalities by Brocard Point This paper takes Brocard Point as a core. We proved some properties about Brocard geometry to confirm its accuracy, and deduce some other properties, and then derive some geometry inequalities by these properties. The content may divide into four parts: a) Derives geometry inequality by Brocard Angle, Crux Mathematicorum and properties which known or deduced. b) Unifies "Fagnano problem", "Fermat Point", "Euler formula" to derive several geometry inequalities. In particular the inequalities between triangle area and length of side, or circumradius inradius and the length of side, is quite interesting. c) Derives geometry inequalities about length of sides in triangle by the distances between incenter centroid circumcenter and Brocard Point. Especially, these inequalities were elegant which derived by incenter and centroid, but it was complicated derived by orthocenter. d) According to the relation about incenter centroid and Brocard Circle derives a series of inequalities. Discover Weitgenberk inequality makes us excited.

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線蟲捕捉菌Arthrobotrys musiformis 調控捕捉網之分化及其黏液之基因之選殖和特性界定

Nematophagous fungi can form different kind of trapping device to trap the nematodes when they show off. They may play a role for control of the plant and animal parasitic nematodes as an alterative choice beside regular practice. We attempt to investigate the adhesive’s attributes and the genes that encode trapping structures. Now we have already constructed the Arthrobotrys musiformis Fosmid library which will play a vital resource for specific genes analysis, cloning and characterization in the future. We have chosen two genes encoding protease and superoxide dismutase from Arthrobotrys musiformis, respectively, and will be used as probes to screen the Fosmid library. The relevent clone(s) will be subject to restrictive enzyme disection, Southern blotting or even whole Fosmid 40kb DNA fragment sequencing to discover the interesting and paramount genes. 線蟲捕捉菌在線蟲出現時可以產生型態各異的捕捉構造,捕捉或黏著線蟲。它是防治線蟲的另類選擇。本實驗是由生物的分生觀點切入,希望能夠了解線蟲捕捉菌Arthroborys musiformis於捕捉網表面之黏液生化特性以及控制產生捕捉構造的基因。目前已完成建 Arthrobotrys musiformis之Fosmid library,並且選擇兩組探針:蛋白質?(protease)以及超歧氧化?(superoxide dismutase),將以PCR進行基因探針之DIG標定,之後篩檢Fosmid library,選殖出相關clone,進行限制?切割,南方氏雜合特性分析或40kbDNA全序列分析,尋找相關基因以利下游實驗工作之進行。

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