動脈硬化是個致病率和致死率相當高的慢性發炎疾病。HMG-CoA 還原酵素抑制劑 纇藥物，簡稱statin, 是一類強效的降血脂藥物，而且在臨床上對於心血管疾病有廣泛的 治療效果。然而近年來的研究報導指出，statin 會有如此廣效治療效果，其原因不僅僅 是因為它的降血脂能力，而是statin 也具有抑制發炎作用的效果。雖然臨床上已經證實 statin 可以減緩動脈硬化的病程，但是statin 對於誘發型一氧化氮合成酵素(iNOS)表現的 調控機制還不明確。有文獻指出NOS 代謝產物nitric oxide (NO)可以改善血流，而可能 在動脈硬化上扮演保護角色。值得一提的是雖然適量 NO 有維持血管恆定的功能，過 量時則會造成血壓過低休克的現象，這就是細菌感染後因內毒素 lipopolysaccharide (LPS) 作用引發敗血性休克的主要原因之一。在本實驗中，我們使用fluvastatin、lovastatin、 pravastatin 和 atorvastatin 這四種statins 來探討它們對於血管平滑肌細胞由LPS 及IL-1β 誘導iNOS 基因表現的影響。我們發現，statin 可以抑制LPS 所誘發的NO 和iNOS 表現， 但卻會促進IL-1β所誘發的反應。NF-κB 在iNOS 的基因調控上扮演重要的角色，而在 探討NF-κB 被LPS 和IL-1β活化的情形中，statin 同樣會促進IL-1β活化NF-κB，但抑制 LPS 活化NF-κB。我們也發現fluvastatin 對於IL-1β所引發NO 的產生、iNOS 的表現、 NF-κB 的活化，以及p65 向細胞核移動的促進作用，在ROCK 抑制劑Y-27632 的處理後 可以看到相同的現象。IKK kinase assay 顯示Y-27632 對於LPS 所促進的IKK 活性影響 很小，但是會促進IL-1β的活化作用。接著，在ROCK 的活性方面，LPS 會抑制原本已 表現的ROCK 活性， 而相反的，IL-1β會增加ROCK 的活性。總括來說，這些結果顯示 ROCK 在血管平滑肌細胞中扮演IKK/NF-κB 的負向訊息調控者角色，而這個機制在LPS 和IL-1β的訊息傳遞路徑中有不同的調控。即ROCK 以負向調控機制角色影響IL-1β的 訊息傳遞，卻不存在於LPS 的情況中。就是因為透過反轉這個負向調控的機制，statins 3 和ROCK 抑制劑對於LPS 及IL-1β引發血管平滑肌細胞的iNOS 表現，呈現相反的調控 結果。這些作用可能參與statin 預防血管再阻塞，抗發炎，抗動脈硬化的作用。此外Statin 抑制LPS 的iNOS 表現作用或許將來可運用於治療敗血性休克。利用基因微陣列分析也 偵測到一些受fluvastatin 正向或負向調控的基因，目前我們正朝鑑定基因的表現改變及 確認其功能，生理意義進行實驗中。 The 3-hydroxy-3-methylglutaryl (HMG)-CoA reductase inhibitors, statins, are potent inhibitors of cholesterol synthesis and have wide therapeutic use in cardiovascular diseases. Recent evidence, however, suggests that the beneficial effects of statins may extend beyond their action on serum cholesterol levels. Although statins have been shown to reduce progression of atherosclerosis, little is known about mechanism by which statins affect iNOS expression. Optimal level of NOS product, NO, possesses the anti-inflammation and anti-proliferation effects in atherosclerosis, while large amount of NO contributes to the septic shock in response to bacterial endotoxin, lipopolysaccharide (LPS). In this study, we investigated the effects of fluvastatin, lovastatin, pravastatin and atorvastatin on IL-1β- and LPS-induced NO production in cultured rat vascular smooth muscle cells (VSMC). We found statins can inhibit LPS-induced iNOS expression and NO production, while they can potentiate IL-1β-elicited responses. In studying the activity of NF-κB, which plays an important role for iNOS gene induction, we found that fluvastatin can increase IL-1β-induced p65 nuclear translocation and NF-κB activity, while inhibit those induced by LPS. The potentiation effects of fluvastatin on IL-1β-induced NO production, iNOS expression, NF-κB activation and p65 nuclear translocation were all mimicked by a ROCK inhibitor, Y-27632. IKK kinase assay showed that Y-27632 itself has minimal effect on LPS-induced IKK activation, while enhances the response of IL-1β. Studies on examining ROCK activity showed LPS can downregulate constitutive ROCK activity, while IL-1β oppositely increases ROCK activity. Taken together these data suggest ROCK is a crucial negative regulator of IKK/NF-κB signaling pathway in VSMC, and this negative control is existing in the action IL-1β, but is absent in the action of LPS. Through abrogating the function of this negative regulator, statins and ROCK inhibitor thus differentially regulate iNOS expression induced by LPS and IL-1β in VSMC. These results suggest that stimulation of iNOS expression in the presence of IL-1β might contribute to the beneficial effects of statins in atherosclerotic process in terms of vasodilation, anti-inflammation and inhibition of smooth muscle cell proliferation. In contrast, the diminishing effect on LPS-induced NO response possibly may provide new therapeutic strategy in sepsis. Al these results strengthen the pleiotropic actions of statins in anti-inflammation and anti-atherosclerosis. Preliminary microarray analysis further revealed several genes either upregulated or downregulated by fluvastatin. The identification of these genes and studying their functional roles in atherosclerosis are currently in progress.

本實驗中我們利用廣鹽性吳郭魚進行氯離子調節機制的研究，探討廣鹽性吳郭魚如何能在不同環境中維持體內氯離子恆定，進而適應生存環境。我門想要探討：『是否NKCC 這種蛋白質在淡水吳郭魚MR 細胞中扮演吸收 Cl- 的角色? 如果是，吳郭魚又如何藉NKCC 的調節適應環境中 Cl- 的變化呢？』我們利用細胞免疫螢光染色法、西方墨點法和共軛焦顯微鏡觀察分析NKCC 在不同 Cl- 濃度人工淡水馴養的吳郭魚MR 細胞上的表現量，結果發現 NKCC 分布於頂端細胞膜(又稱為細胞開口)，及其附近的細胞質內；環境中 Na+ 濃度的差異對NKCC 在MR 細胞上的表現影響不大，但低 Cl- 環境馴養的吳郭魚，NKCC 表現量都高出其他組很多。顯示NKCC 參與了氯吸收的機制。另一個實驗中，我們將吳郭魚由淡水中轉移至海水以分析它們在適應海水的過程中NKCC 的表現變化。結果發現在馴養初期（16 小時內），圓點狀NKCC 仍然可以在MR 細胞的開口附近觀察到，但到了24 小時後，NKCC 在開口的表現就明顯減少甚至消失，取而代之的是轉移到底側邊細胞膜上的NKCC。此實驗證實了NKCC 這一個與Cl-運送相關的蛋白質，在廣鹽性吳郭魚氯離子調節中扮演了很重要的角色。 Euryhaline tilapia (Oreochromis mossambicus) is capable of maintaining internal ion constant ineither hypertonic or hypotonic environments (fresh water or seawater).MR cells in the gills of tilapia play critical role in absorbing Cl- from fresh water or pumping redundant Cl- from body fluid into seawater. Chloride transporter (NKCC) which distributed in basolateral membrane of MR cells is involved in Cl- secretion of seawater teleost. However, the mechanism of Cl- absorption in fresh water MR cells is still unclear. Whether NKCC is also involved in Cl- absorption and how do tilapia regulate Cl- absorption are the questions this study aim to answer. By using immunofluorescent staining, western blot, and confocal microscopy, the distribution and expression level of NKCC in fresh water MR cells were examined. We found that NKCC is distributed on the apical membrane of freshwater MR cells where is known to be the site for active Cl- absorption of MR cells. We compared the expression level of NKCC in MR cells from tilapia acclimated in high, normal, and low Cl- artificial water for 7 days. The results showed that NKCC is induced by ambient low Cl- , and in contrast suppressed by high Cl- water, indicating NKCC might be involved in Cl- absorption of freshwater MR cells and up-or down-regulated to maintain Cl- uptake constant. In addition, we also examine the expression pattern of NKCC in MR cells from tilapia transferred from fresh water to seawater. Confocal images show that apical expressed NKCC disappear gradually within 24h seawater acclimation and is substituted by basolateral expressed NKCC. This study provides a novel regulatory mechanism of NKCC in Cl- transporter of MR cells.

本研究的目的在於設計出一機械裝置，使振翅翼的升力比傳統的設計能更有效率的提升，並經由實驗證實此種機械裝置理論的正確性。在觀察有關鳥類飛行過程的錄影帶及尋找鳥類相關的資料且界定欲實驗的種類和現有的能力及資源後，本研究中設計出三種可行的振翅翼機械裝置，並進行在相同風速、相同振翅頻率(每分鐘振翅翼拍動144 下)的條件下，測試在不同振翅翼機械設計條件下的升力系數，在實驗中發現既有而廣泛的應用於玩具上的振翅翼設計與固定面振翅翼的設計，二者在拍打的過程中各有優缺點，而第三種的半活動面振翅翼設計，在拍打時能排除前者的缺失而同時擁有前兩種的優點。此種半活動面振翅翼機械裝置大大的提升了振翅翼的升力，且在設計上與現有的形式差異不大，若能更進一步的在機構上做更精密的改良設計，則將可大大提昇現有振翅機的性能。The study on the flapping wings with various angle of attack and different wing designs have been done. Three types of wing have been examined in a home-build wind tunnel . One is a membrane wing with fixed leading-edge support, the second is a membrane wing with both fixed leading-edge and fixed-web support , the third wing is a membrane wing with fixed leading-edge support and half-free-joint web support , with varions web length and web curvature under different angle of attacks , the wind tunnel testing results show that the half-free-joint web support membrane wing with fixed leading-edge can offer a much higher lift coefficient . It is suggested that the third type wing is the best choice for designing the future flapping wing vehicle.

NO.57-07 2018 SEP | 科學研習期刊目錄 本期專題 科學遊戲大家談 科學遊戲「大家」談 | 許良榮 再生材料融入科學創客活動之教學經驗分享 | 許兆芳 從童玩談自造科技 | 王德麟 靜電遊戲 | 陳坤龍 在實驗小學玩科學遊戲 —以岳明國小為例 | 盧俊良 紙愛科學DIY | 謝迺岳 玩碳奈米材料遊戲 —以辦理國小奈米寒假營活動為例 | 林志明、李紫原、唐文華、張美玉 森棚教官的數學題 公園跑切線 | 游森棚 科學話題 日本風神居住地 - 鳥取沙丘 | 梁勝雄 科學讀書會 思考的演算：跟著電腦學思考，你也可以成為計算思考大師 | 白榮銓 總召集人的話 有「愛博士」（Dr. Love）美稱的美國南加大特教系已故教授 Leo Buscaglia（ 1924-1998）曾說：「很弔詭的是，許多教育人員和家長們仍然將學習時間和遊戲時間區分開來，而沒有看到它們之間的重要連結」。科學研習和遊戲該妥為連結。本期特別以「科學遊戲『大家』」談」為專題，請國立台中教育大學科學教育與應用學系許良榮教授擔任特約主編。 專題文章含專題導言共有七篇：「專題導言」〈科學遊戲『大家』」談〉從科學遊戲已受到國人關注但需以嚴謹的方法進行論述、討論或研究切入，並簡介其它六篇專題文章。〈再生材料融入科學創客活動之教學經驗分享〉一文透過教學實務案例，分享如何選取再生材料與活動引導歷程，讓學生從實作中累積經驗，應用科學知識解決問題。〈從童玩談自造科技〉一文借助軌道組和音樂鈴兩種自造科技，介紹其科學原理和科技與工藝概念。〈靜電遊戲〉一文透過五個實驗利用生活中簡單的道具製造不同的靜電效果，藉以說明靜電產生的原理及特性。〈在實驗小學玩科學遊戲—以岳明國小為例〉一文分享科學遊戲運用在實驗小學自然教學的經驗。〈紙愛科學DIY〉一文分享10 種（摺、撕、驗⋯⋯）紙的趣味教學。〈玩碳奈米材料遊戲─以辦理國小奈米寒假營活動為例〉一文介紹可帶領學童認識自然界中碳奈米現象，體現以玩碳奈米材料遊戲的教學模組。 「森棚教官的數學題」刊登〈公園跑切線〉一文，拋出在正三角形公園內的正三角形綠地外跑切線問題，供讀者自主探索或發想科展題目。「科學話題」單元刊登〈日本風神居住地─鳥取沙丘〉一文，介紹位在日本中國地區的鳥取縣一片廣大、狀似「沙漠」的地形景觀，是如何形成的？為什麼當地人一面保護這一片「沙漠」深怕它消失，一面又怕它擴大影響生活？ 十二年國教課綱中，資訊科技課程是以運算思維為主軸。但是，何謂運算思維？包含那些技能？如何應用來解決問題？本期「科學讀書會」導讀《思考的演算：跟著電腦學思考，你也可以成為計算思考大師》一書，該書搭配遊戲、謎題、魔術以及電腦科學家正在研究的真實難題，解釋何謂計算思考（即運算思維），以及相關的各種技巧。 此外，本期是本刊今（2018）年7 ∼ 8 月暑假期間休刊後的9 月復刊號，自本期起本刊僅有官網全本電子書及單篇 PDF 檔的美編版，不再印、寄紙本。 總召編輯委員 - 李隆盛 關於本刊 出版單位：國立臺灣科學教育館 發行人：陳雪玉 總召集人：李隆盛 編輯委員：古建國/全中平/李文獻/李旺龍/李源順/李進寶/林沛練/林坤誼/許良榮/蔣中柱/胡秀芳/陳正改/陳竹亭/陳玫良/楊水平/游森棚/謝隆欽/張子超 策劃：曾聰邦 主編：錢康偉 本月專題特約主編：許良榮 編輯：張志定/吳郡怡 網頁設計編輯：施曉恬/陳璽君 投稿規範請來信詢問：article@mail.ntsec.gov.tw

有鑑於SARS 期間活性碳口罩因原料均從國外進口，而造成活性碳急需用時的短缺，於是我們的研究是以國內最常見的木材自製成活性碳，並探討活性碳對生活週遭常見物質的吸附能力。我們利用六種不同的木材配合兩種活性化方法來製成不同的十二種活性碳，觀察活性碳對有色溶液的吸附效果，發現不同的活性碳對不同的物質有不同的吸附效果。在定量實驗中，我們採用不同大小、不同性質的物質依次為氯離子、氨分子、葡萄糖分子及三氧化二砷等，用十二種不同的活性碳作吸附效果的比較，發現每種物質都有對其具有最佳吸附能力的活性碳。再利用電子顯微鏡觀察並測出活性碳孔洞的大小，將結果與活性碳對各種物質的吸附能力相對應而得知，【1】活性碳的孔洞大小與物質質點相容性大即得最好的吸附效果【2】雖然木材本身並不帶電，但其中所含的礦物質可能會具有電性，所以在必要情況下亦可將電性列入考慮。同時，在現代化的生活環境中，人們與電磁波的接觸已無可避免；而電磁波因頻率的差異而有不同的特性，因此對於人們的影響也不相同，新聞報導中常出現宣稱竹炭具有遮蔽電磁波的效果。於是我們深入地去探討竹炭對電磁波的影響原因。 在我們實際測量過程中發現，基地台、高壓電塔、行動電話和家庭電器所釋放之電磁波總和均在安全範圍之內。竹炭的燒製溫度須達550℃以上才可在內部形成具有導電性的石墨結構才進一步具有屏蔽效果。 ;During the ravage of SARS, a great amount of activated carbon was imported to Taiwan, leading to the shortage of actived carbon for emergent use in our country. In view of this phenomenon, we investigated six kinds of wood that are most commonon the market and processed them into twleve kinds of actived carbon. We found that different actived carbon has distinct adsorptive power on different substances by observing theadsorption in the colored solutions. In quantitative experiments, we applied substances of different sizes and properties (chlorine ions, ammonium ions, glucose molecules, arsenic(Ⅲ) oxide) to compare the adsorption of these twelve kinds of actived carbon. We found that each substance is adsorbed the most by a certain kind of actived carbon. Then we observed the apertures of actived carbon under stereoelectric microscope and contrast the result with adsorption. We had two following findings: Actived carbon has better adsorption if its apertures fit the adsorbate molecule well in size. Wood itself doesn’t have electric charge. But sometimes the minerals contained would enable it to have electric charge. We have to take the electric charge into consideration in the experiment if necessary. At the same time, in a modern life, the contact between us and electronic waves are not avoidable. Electronic waves with different frequency have varied characteristic and therefore cause dissimilar influences on humans. The news reports keep on showing the charcoal can shield us from electronic waves. Therefore, we want to find the causes why the charcoal affects the electronic waves. During the process of the research, we find that the total of all electronic waves released by electricity tower, mobile phones, household electronics are within the secure spec. The charcoal has to be burned higher than 550℃ and then it can generate the electric conduction graphite structure inside and then it can have the shielding function.

本研究工作自2001 年7 月至2002 年6 月止，在臺北市內湖區金面山進行地衣實地探勘採集調查，以位於臺北市內湖區之麗山高級中學之校園為中心點，範圍由校園金面山至學校前門所面對的港墘路與環山路口。應用「外部型態比較」，「切片觀察(徒手切片技術)」與「化學分析(薄層色層分析Thin Layer Chromatography, TLC)」等三種方法，鑑定所採集之地衣所屬種類，並探討空氣污染程度對金面山地衣類分佈狀況的影響。調查至2002年6月為止，本研究已發現生長於金面山區之數種地衣，其中殼狀地衣包含有：癩屑衣屬(Lepraria)、球粉衣屬(Spheophorus)、大孢衣屬(Megalospora)等三屬；屬於莖狀地衣的僅有石蕊屬(Cladonia)；為葉狀地衣的則有：黃梅衣屬(Xanthoparmelia)、裸緣梅衣屬(Parmotrema)、梅衣屬(Parmelia)等三屬，合計共七個屬。本研究針對臺北市金面山區及麗山高級中學之校園附近市區道路地衣類分佈的情形做調查，結果發現行道樹上之附生地衣在較靠近山區的地方，於樹幹上的生長覆蓋面積才會有明顯增加的情形，又其中在市區道路上只發現殼狀地衣生長，而莖狀及葉狀地衣則出現於金面山頂附近，所以就此情況而言，除了金面山區外，市區已呈現地衣沙漠化之狀況，間接顯示，市區的空氣污染是相當嚴重的。本研究將所調查結果之各屬地衣類進行特徵描述與分佈地區之標定，以便於日後臺北市內湖區金面山地衣之鑑識及後續相關研究之進行。This research was carried out between July 2001 and June 2002 on the Jinmian Mountain in Neihu,\r Taipei City and consisted of an on-the-spot exploration and collection of lichen. The Taipei Municipal\r Lishan High School was the center of this research, and the researched territory consisted of the areas\r between the mountain at the back of the school (Jinmian Mountain) and the intersection between\r Gangcian Rd. and Huanshan Rd. in front of the school’s main gate. Comparison of external appearance,\r dissectional observation (manual breaking technique), and chemical analysis (Thin Layer\r Chromatography, TLC) were the research methods applied to determine the genus of the lichen and the\r influence of the degree of air pollution on the distribution of the lichen.\r By the end of the research in June 2002 the presence of three types of lichen on the Jinmian\r Mountain was observed: crustose lichen: Lepraria, Spheophorus, Megalospora; fruticose lichen: Cladonia;\r foliose lichen: Xanthoparmelia, Parmotrema, Parmelia. Seven different genus of lichen have been found.\r The research has been done on the distribution of these different genus of lichen in the area of the\r Jinmian Mountain and The Taipei Municipal Lishan High School: By observation the sidewalk trees\r near the mountain’s area, we found the covering area of the lichen on the sterns apparently increases,\r while on the trees in the city district, only crustose lichen grow. The fruticose and foliose lichen only\r grow near the top of the Jinmian Mountain. This impoverishment of the diversity of lichen in the city\r district might (indirectly) be an indicator of the considerable decline of air quality in the city. The\r outcomes of this research regarding the description and distribution of the lichens contribute to the\r knowledge on lichens in the Jingmian Mountain area in the Neihu District, and may raise the similar\r research in the future as well.

Feeding behavior is the action which animals depend on maintain livelihood. Snakes usually use the three following ways to catch their preys: winding, venom-releasing and pressing their game to death. However, previews study is rare about the feeding behavior of Ramphotyphlops braminus. This puzzles us, prompting us to do in-depth research on it. When performing an experiment, we will use the camera with infrared function to record entire experiment and the obtained data will transform the graph. Our result shows the feeding behavior of R.braminus is a new way to catch their game, and the minute process of this special way is also written down in our report. We hope that the result can let everyone be clear about Ramphotyphlops braminus of soil ecosystem status, and it is an essential contribution for building the archive of Family Typhlopidae. 攝食行為是動物賴以維生的行為。在蛇類中，常見的捕捉方式有：纏繞、 使用毒液、壓斃等三種類型。但，文獻中卻沒有任何有關於鉤盲蛇(Ramphotyphlops braminus)的捕食行為。這使我們感到疑惑，並想深入探討。在實驗進行當中，我們使用紅外線攝影機將實驗全程錄製下來，並將所得的數據轉化成圖表。而其結果顯示鉤盲蛇(Ramphotyphlops braminus)的捕食行為是一種全新的模式，這種模式的詳細過程也被我們全部收錄到報告中。我們希望做出來的結果能讓大家對鉤盲蛇(Ramphotyphlops braminus)在土壤生態系中的地位更加了解，而且對於建立盲蛇科(Typhlopidae)資料庫有實質的貢獻。

Nematophagous fungi can form different kind of trapping device to trap the nematodes when they show off. They may play a role for control of the plant and animal parasitic nematodes as an alterative choice beside regular practice. We attempt to investigate the adhesive’s attributes and the genes that encode trapping structures. Now we have already constructed the Arthrobotrys musiformis Fosmid library which will play a vital resource for specific genes analysis, cloning and characterization in the future. We have chosen two genes encoding protease and superoxide dismutase from Arthrobotrys musiformis, respectively, and will be used as probes to screen the Fosmid library. The relevent clone(s) will be subject to restrictive enzyme disection, Southern blotting or even whole Fosmid 40kb DNA fragment sequencing to discover the interesting and paramount genes. 線蟲捕捉菌在線蟲出現時可以產生型態各異的捕捉構造，捕捉或黏著線蟲。它是防治線蟲的另類選擇。本實驗是由生物的分生觀點切入，希望能夠了解線蟲捕捉菌Arthroborys musiformis於捕捉網表面之黏液生化特性以及控制產生捕捉構造的基因。目前已完成建 Arthrobotrys musiformis之Fosmid library，並且選擇兩組探針：蛋白質?(protease)以及超歧氧化?(superoxide dismutase)，將以PCR進行基因探針之DIG標定，之後篩檢Fosmid library，選殖出相關clone，進行限制?切割，南方氏雜合特性分析或40kbDNA全序列分析，尋找相關基因以利下游實驗工作之進行。

台灣溫泉資源豐富，不同的溫泉泉質適合不同的溫泉藻類生長。溫泉藻屬於極端生物，其應用資源極待研究開發。本研究根據細胞型態、藻膽蛋白種類分析以及rbcL 基因定序等結果推測北投溫泉藻屬於一種溫泉紅藻，其種類近似於Galdieria sp. (Rhodophyta)。此外，本研究利用葉綠素螢光分析儀於野外實測北投溫泉紅藻於不同光強度下，光合作用能力的差異，發現適應於不同光強度的溫泉紅藻，其電子傳遞速率並無顯著的差異。推測其原因可能與北投溫泉紅藻的phycocyanin 含量會隨著光量有所調整，藉此達到最佳的光合作用能力有關。另外，本研究發現隨著光強度的上升，溫泉藻的NPQ 值有上升的趨勢，然而低光區的溫泉藻有較高的NPQ 值，此與一般理論不符。利用HPLC 分析北投溫泉紅藻的類胡蘿蔔素種類組成，其種類分別為Lutein、α-carotein、β-carotein 與Zeaxanthin，未發現Violanxanthin 與Antheraxanthin，北投溫泉紅藻並不俱有葉黃素迴圈。因此根據研究結果，我們認為NPQ 值與色素的關係仍有討論的空間Taiwan is resourceful in hot springs. Various hot spring algae are adapted to different types of hot springs. Hot spring algae applications are still under developing. In this work, based upon the morphology, analysis of phycobiliproteins, and rbcL sequences, the hot spring algae of Peitou is found to be Galdieria maxima (Rhodophyta). In addition, the dependence of the photosynthesis of Galdieria maxima on the light intensity was measured by Diving-PAM. The results show that the light energy availability efficiency of Galdieria maxima adapting to different light intensity exhibited different, although the electron circulate rate differed insignificantly. This suggests that the concentration of phycocyanin in Galdieria maxima may vary to attain optimal photosynthesis. Furthermore, the NPQ of Galdieria maxima increase with the light intensity. However, contradict to the theory; even under the same light intensity, the NPQ of Galdieria maxima was higher at low light zone. The carotene composition of Galdieria maxima was analyzed using HPLC and found lutein, α-carotene, β-carotene, and zeaxanthin. Violaxanthin and antheraxanthin were not present. Therefore, Galdieria maxima do not exhibit xanthophyll. Based on the results of this study, the correlation between NPQ and pigment still needs to be investigated.

震盪反應有許多種反應類型，至今已被完整探討，但對於震盪反應之催化系統，卻鮮少有文獻提及，本研究小組偶然發現，醇類對於震盪反應具有明顯的催化作用，本文嘗試探討各級簡單的醇類對於震盪反應影響，並透過活化能的改變了解醇類在催化過程中所扮演的角色。The many types of BZ oscillation reaction have already been thoroughly discussed. However, little mention has been made in literature regarding the catalysis system of the reaction. The researchers have accidentally found that alcohols exert an obvious effect on the oscillation reaction. The study attempts to discuss the different influences that various kinds of alcohols have on the oscillation reaction and to understand the role alcohols play in the catalytic process through the change of activation energy.

二元根樹是資訊科學上最重要的結構之一，因此其結構的探討就很有價值。在這個研究中，我們探討二元根樹上反鏈(Antichain)，鏈(Chain),獨立集(Independent set)計數。在每一個子題之下，我們求出計算反鏈，鏈，獨立集的方法。之後我們求出極值(包括最大跟最小值)，並且討論該極值之下所有可能的樹形。此外，我們並討論要列出二元根樹上這些資料(反鏈，鏈，獨立集)所需的演算法。In this paper we enumerate the number of antichains, chains, and independent sets of a binary tree of size n. After that we focus on the extreme values (maximal/minimal). we calculate the extreme values and discuss the possible graphs of trees when achieving these extreme values. Moreover, we offer algorithms for listing all the objects in each extreme case.