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人類為了要了解或描寫自然現象,常採用一種「逼近」的過程來處理問題。比如某位同學使用一尺來量取一線段長幾次,結果產生每次的讀數都不相同,此時產生逼近解的問題。又比如有四支 50 公分左右的尺,每次使用兩支來量取一公尺左右的線段長,亦產生類似的問題。然而這些「逼近」解的可靠性到底如何?我們則用方差來權衡之。
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YWHAZ(14-3-3ζ)蛋白在肺腺癌細胞中可能活化Wnt路徑之探討
YWHAZ(14-3-3δ)蛋白被證實在肺腺癌中會引發上皮-間葉的轉變作用(epithelial - mesenchymal transition ; EMT)導致癌轉移,然而其分子機制卻不清楚。先前實驗室利用cDNA微陣列技術(microarray)發現肺腺癌細胞大量表現YWHAZ蛋白會導致Wnt訊息傳遞路徑之相關基因大量表現。因此,本研究目的是探討YWHAZ蛋白在肺腺癌細胞中是否會活化Wnt途徑而引發EMT。\r 本研究結果發現大量表現YWHAZ蛋白除了會增加細胞質中β-catenin的表現量,也會增加細胞核內 β-catenin 和TCF4 的蛋白表現量,並且在細胞核中與β-catenin和TCF4形成複合體;而臨床肺腺癌病患檢體中發現,相較於正常肺葉組織,YWHAZ蛋白與β-catenin在肺癌組織的檢體有較高的表現量,同時少部分會進入細胞核內。經由以上結果,我們推測YWHAZ蛋白可能會結合β-catenin與TCF4蛋白並活化Wnt途徑,因而引發EMT之產生,而促進肺癌轉移。說明YHWAZ蛋白有潛力成為日後診斷肺腺癌的指標蛋白(marker protein),同時亦成為治療肺腺癌的標的蛋白(target protein)。\r 重要性\r 1.提出YWHAZ可能在肺腺癌細胞中活化Wnt訊息傳遞路徑(Wnt signaling pathway)而導致癌轉移。\r 2.再度指出YWHAZ在腫瘤生物學的重要性。\r 3.對於肺腺癌轉移提供了臨床診斷及治療的另一種選擇 ─ YWHAZ。
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有一個科學小遊戲,將濾油網對向光源可看到美麗的光譜。我們對此現象感到好奇。國中自然課本中有一個色光的實驗,將白光透過玻璃紙的濾光和色紙的反射,可看到不同的顏色,我們的實驗結果卻不符合課本內容。於是想用光柵片來分析色光,探討實驗的問題。我們實驗的結論如下。雷射光是單色光,其它的光源如太陽光、日光燈和LED燈都不是單色光。玻璃紙的濾光功效不完全。色紙反射白光的功效和角度有關。以兩條上下並行的狹縫和光柵片所產生的光譜進行混色,得到與課本相同的結果。利用光柵繞射方法,可以求出蒸餾水的折射率,對照利用入射角與折射角求折射率的傳統方法,所得到的結果相當一致。
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條件反射是什麼呢?條件反射為生理學中的一種行為過程。在特定環境中,因強化作用而使某種應答的出現頻率增加,且更為規律。所謂強化是指為求得預期的應答而提供一種天然刺激或人為獎賞。在這個實驗中,我們欲探討「生物在不同環境中學習行為表現將有何不同」,於是觀察紅球魚、黑尾太陽魚與孔雀魚在接受餵食時的行為,並持續注意其長期所表現出的現象,最後結論是不論任何魚種條件反射之學習曲線相近,再學習情形皆明顯加快。
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上數學課的時候,老師教我們如何計算體積和容積。為了加深學習的印象,同學們利用下課的時候,計算各式各樣文具及簿本的體積,並利用上完課的大字報,摺出各式各樣大小的紙盒,來貯存各式各樣的文具及簿本。但是經過幾天的觀察後,卻發現這些紙盒並不實用,不但不方便攜帶,而且也很容易破損。正當大家陷入沉思的時候,突然有同學發現,佈告欄上的林林總總的宣傳海報和回收的壁報紙,似乎比大字報更耐用,如果再加上提把的設計,就可以帶著走,不但可以資源再利用,而且更貼近生活需要。在經過不斷的努力蒐集資訊及不斷觀察、試驗和改良後,終於利用學校佈告欄的回收紙,設計出符合自己使用需求的紙袋。
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以阻抗匹配調整太陽能最大功率輸出之研究
In recent years, the price of the oil keeps rising continuously. As a result, the prices of the commodities are rising, too. But what does this mean? This situation stands for the resources on are becoming more and more valuable. A few months ago, I read a Weekly Business Magazine and a Scientist Magazine. They both pointed out that the resources such as oil would disappear after fifty years, and that was a horrible phenomenon. Since almost everything in our modern lives are related to oil, like automobile, motorcycle and air plane. They all need oil for its ingredient. Even plastic bag plays a part in the products of it. I really cannot imagine what it would be like if we don’t have oil after fifty years. Owing to the green house effect is becoming more severe, there are many substitution resources found, such as hydraulic power, wind power and solar energy…etc. What we’re discussing in our topic is how to enhance the power of solar energy, because for now, we all know that the solar module is very expensive and it cost a great deal of money just only one square meter, but the price of module converted from sunlight or heat energy can’t be higher. Therefore, the work is mainly to design and carry out a solar max power point track. 近年來,油價不斷的上漲,連帶著民生的物價漲幅也是越來越可觀,但…這意味著什麼 呢??這所代表的是地球上的資源可以說是越來越珍貴了,前陣子曾經看過商業週刊、科學人 雜誌…他們紛紛所指石油這種能源可能在五十年後就消失殆盡,這個可是非常可怕的結果。 鑑於溫室效應愈趨嚴重,許多的替代能源紛紛出籠,像是風力、水力和太陽能等等。我們這 次的主題是在討論如何提高太陽能效率,現在的太陽能模組我們都曉得非常的昂貴,但是由 太陽轉化成電能的效率卻是低的可以。所以本作品主要目的在於設計與實現一個太陽能最大 功率追蹤器。
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係因市面上的豆漿都標榜高營養價值,本實驗將探索如何製出易於人體吸收的豆漿,即泡製豆漿的條件如能讓黃豆酵素充分作用,將有利豆漿中大分子轉為小分子。 本實驗以不同浸泡時間、溫度、pH值、添加物來探討澱粉?和蛋白?的作用,結果顯示在乾燥黃豆泡水26.5小時內,澱粉?表現極佳,蛋白?則表現很少:澱粉?在23~26小時、50℃、加入添加物綠茶粉、維生素C、葡萄籽、鐵劑作用最佳,因此泡製過程中依此條件將可助豆漿的澱粉轉為葡萄糖,蛋白?在35℃作用效果略佳。如要直接攝取豆漿中的胺基酸,黃豆須先催芽一天酵素才能釋放分解蛋白質;在豆漿中加入乳酸菌,幫助蛋白質的分解。應用此項結果能製造較易於人體吸收的豆漿。
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本研究利用塑膠投影片、吸管、黏膠等材料,以自製的簡易風洞測試單片翼的升力與水平推力,進而探討不同變因對雙滑管、連結雙支架之翼片爬升力量的影響,成功使用三滑管連結兩組雙支架製成一個能伸展與收縮、有節奏向上爬升的M型毛毛蟲翼片。我們又改變M型毛毛蟲的大小比例,在不同的風速下分別測試其爬升速率,發現毛毛蟲越大隻,其爬升平均速率受風速變化的影響越明顯(爬升速率變化趨勢線斜率越大)。最後我們嘗試增加背腹翼的寬度,以增大翼片面積提高升力,成功做成一隻能伸縮爬升的雙M型(三節式)毛毛蟲。
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Recovering bioethanol from fruit wastes
1. Purpose of the research: Recovering bioethanol from fruit wastes by using brewing yeast and enzyme Viscozyme L\r 2. Procedures: Four different experiments were conducted in our project.\r Experiment-1: Samples in RM by the addition of only S.Cerevisia.\r Experiment-2: Samples in RM broth meduim by the addtion of 100 μl/g ViscozymeL and S. Cerevisiae\r Experiment-3 : ViscozymeL with different concentration\r Experiment-4: to determenine whether the enzyme with high concentration inhibited the fermentation.\r Analysis of ethanol: HPLC method was used for the analysis;\r HPLC method: (high performance liquid chromatography)\r 3. Data\r HPLC measured initial and final concentrations of glucose and ethanol by the addtion of S. Cerevisiae.\r Initial glucose concentration and final ethanol concentration was the highest in Grape pulp 12 and 9 g/L respectively. Despite the glucose concentration was the lowest, the ethanol concentration was quite high (7 g/L) in the mixture with orange. The lowest ethanol was produced in the mixture with pomegranate.\r Concentrations of ethanol and glucose from the samples in RM broth meduim by the addtion of 100 μl/g ViscozymeL and S. Cerevisiae\r After the 24 hour incubation by the addition of enzyme glucose concentration incresed by 1, 4 and 2 respectively in sample 1, 2 and 3. The ethanol concentrations in comparison to first experiment were higher especially in the sample1 (grape pulp).\r Effect of ViscozymeL with different concentration on glucose production.\r The glucose concentration generally increased by the addition of 200 micro L of enzyme as the time proceeded. In grape pulp sample glucose concentartion didnt increased by the addition of 500 micro L enzyme.\r The remaning glucose and produced ethanol concentrations by the addition of viscozyme L with two different concentartion and S. Cerevisiae\r As the concentartion of enzyme added incresed the amout of ethanol also inreased in sample 2 and 3 but in the sample 3 the fermentation was inhibited.\r 4. Conclusions\r In this study sugars in fruit wastes that are regularly not recylecled were fermented successfully into ethanol by using S. Cerevisiae.\r Generally we get more ethanol from the samples when the enzyme was used. As the graph5 shows the ethanol concentration generally increased as the enzyme concentration increased. But especially in the sample1 (mixture with pomegranate) at 200 and 500 micro liter concentrations, ethanol production were 2 and 0 g/L respectively. In the light of this daha we conluded that the enzyme with high concentartion might inhibit the fermentation. When the activity of enzyme was inhibited by keeping the pretreated enzyme in boiling water, the fermentation restarted and recovered more ethanol; 8 and 12 g/L at the enzyme concentration of 200 and 500 microliter respectively.
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我們從報紙、雜誌及華視“跳動七十二”節目報導中知道,目前許多食品均以硼砂、硼酸做為防腐劑,如果長期攝取,將導致硼的蓄積,便人體引起紅斑、嘔吐、腹瀉、循環系統之破壞、休克、昏迷、腦驚厥等病症,因此引起我們的好奇,硼砂、硼酸真的這麼可怕嗎?
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Few plants can grow in the place where Wedelia trilobata grows. In the following experiment, the leaves ans stalks(of the plants from Wedelia trilobata) are extracted for experimental purpose .The data and records of the experiment have shown that Wedelia trilo ata indeed refain the growth of plants such as mung bean, teeding seed, yeast’s respiration and the photosynthesis. We use the function of dieing the polyphenols to know that the Wedelia trilobata can produce Allelochemicals called as the polyphemols The mesophycell produce the polyphemols from chloroplast of cortex,the polyphemols transport to root and release from root exudation. The plant will absorb polyphemols of the soil, and will affect the growth of the plant. If we can separate and purify the Allelochemicals, we can make it as natural herbicideb. 南美蟛蜞菊所在之處,少有其他植物,本實驗採南美蟛蜞菊植栽區之土壤與南美蟛蜞菊莖或葉之萃取液進行相剋作用之研究,結果顯示南美蟛蜞菊確實會抑制小麥與綠豆種子萌發及幼苗生長,並抑制酵母菌的呼吸作用及植物光合作用中之光反應。 利用多酚類染色方法得知南美蟛蜞菊產生的相剋化合物為多酚類,且該多酚類由葉肉細胞與莖內皮層中具葉綠體之細胞產生,經韌皮部輸送至根,再由根經泌濾作用釋出。土壤中的多酚類會被植物吸收,進而影響植物的生長與發育。 若能將該相剋化合物---多酚類分離與純化,或可作為具選擇性之天然除草劑。
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