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聚對苯二甲酸乙二醇與聚乳酸共混材料之降解研究
本實驗目的在於嘗試降解傳統塑膠材料。實驗中選用極為常見的傳統塑膠,聚對苯二甲酸乙二醇ploy ethylene terephthalate (PET),與生物可分解材料,聚乳酸poly lactic acid (PLA),將兩者以不同比例物理性混摻,並以豬胰線脂肪酵素Lipase from porcine pancreas, TypeⅡ (PPL)進行降解,期望藉由生分解材料可被完全分解的特性牽引傳統塑膠材料進行降解。\r 本實驗共分兩階段:第一階段實驗中,將共溶劑揮發製膜進行降解,但由於溶劑法所留下的孔洞,影響了降解速率;因此,在第二階段實驗中,改以熱壓的方式製膜解決孔洞問題,並於降解後以儀器分析降解情形。結果發現:混合薄膜經降解後熔點有下降的趨勢,此可佐證確實可以生分解材料PLA牽引傳統塑膠料PET進行降解,且降解速率和結晶情形和分子鏈段纏繞程度有關,在PET與PLA濃度同為3%時降解情形最佳。
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日照影響花芽分化及生長試植物生理學上的重要課題,根據相關文獻指出,蛋白質類型的變化是影響開化的重要關鍵,大豆是短日照植物,分別以長日照(LD18hr/DAY)和短日照(SD6hr/DAY)處理,抽取葉片中的蛋白質以SDS-PAGE.2D-SDS-PAGE分析,SDS-PAGE的實驗姐果顯示出在分子量25.2 32.9 38.6 20.6 64.0 100.6 kDa處中的蛋白質SD比LD的量多,但是在分子量16.8kDa以下的蛋白質LD含有高濃度而自SD只有一點,我們使用2D-SDS-PAGE做進一步的分析,SD與LD分為三個區塊討論(SD1-3 LD1-3)有許多蛋白自LD1.LD3出現,但在SD1 SD3中未出現,可能有抑制花芽分化的作用,而在SD2中有多個高濃度蛋白出現,可能在花芽分化上扮演重角色,其中編號P1 P2的蛋白質有較明顯的變化,根據NCBI網站上的資料顯示,P1蛋白質與光敏素C有關,而P2蛋白質則沒有找到分子量等電點類似的蛋白質;The effects of photoeriod on floral initation and develoment is an imporant phyiological subject and have studed ve he past 70 years. Accrding to the reference, polypeptide partten plays an important rolein photoperiodic induction. Soybean s a SD(short day)plant. So we planted them with 6-h(short day,SD)and 18-h(long day,LD)photoperiods. Total proteins n leaves of soyban were extracted from the vegetable,SD and LD. And using SDS-PAGE and 2-D eloctrophoresis to analyze. 25.2,32.9,38.6,50.6,64.0,and 100.6kDa polypeptides in SDS-PAGE were always present in leavess at SD but only a little bands f the polypeptides appeared at LD. And many polypeptides whose molecular weight(Mw)is below 16.8 kDa had higher concentration at LD ad Reduced at SD. We found three different areas(SD1~3,LD1~3) and analyze them. Many proteins were found n LD1,LD3 and reduced in SD2,SD5. Possibly, these polypeptides found in LD1 and LD3 play an important role in restraining floral iniiation in soybean. Many proeins had more concentration in SD2. Probably, they play an important role in photoperiodic induction and floral initiation. We found a protein labeled P1 in SD2 and it is with molecular weight(Mw) of 36kDa at isoelectric point(pI) of5.2, and it may be related to cytochromeC. We also found protein labeled P2 in SD2 and it is with molecular weight(Mw) of 40.2kDa at isoelectric point(pI)of 5.4, and we couldn't find any protein that has the similary Mw and pI in potein database about flowering and daylength.
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