1. Introduction In Japan the energy self-efficiency is very low: only 6%. Hydrogen (H2) has been expected as a new and alternative energy source to imported one, such as petroleum resources. Now hydrogen energy comes into the practical use in the field of the fuel cell. Hydrogen must be extracted from other sources, for example, water, fossil fuel, and so on. Hydrogen is obtained from water by using electronic or thermal or photo energy in most cases, whereas it is well-known that hydrogen is given by the oxidation reaction of silicon in alkaline aqueous solution: Si + 2OH- + H2O → SiO32- + 2H2 Free silicon (Si) is not only used in the steel refining, aluminum-casting in the field of fine chemical industries but also is used as a material in semiconductor electronics. However, a lot of used silicon is thrown away as a waste, being not reused and recycled. In this study we try to apply a waste silicon to obtain hydrogen based on the above reaction. The purpose of the study is to develop a safe and convenient manufacturing method to generate hydrogen for an energy source of the fuel cell.

本研究以蒙日定理「平面上三圓彼此的外公切線交點共線」及其對偶定理「平面上三圓彼此的內公切線交點與另一圓的圓心的連線共點」出發，探討三圓更多由內、外公切線所產生的共點共線性質，進而探討四圓以上的情形，以及正多邊形、圓錐曲線等位似圖形，並推廣至空間中的球體。正如本研究作品名稱，我們將鮮少人研究的蒙日定理萌發出新枝，在日夜中茁壯，甚至最後有驚人的發現「在空間中n個外離的球，任意1個球的球心與另n-1個球的蒙日點連線會共交一點，此點稱之為n球的蒙日點」，此「點」發現，讓人不禁對宇宙中星體之間的關係產生更多無限的想像。

Retinoblastoma (RB) is a childhood retinal cancer caused by mutations in the RB1 gene. Molecular diagnosis is crucial for early detection and treatment. Current DNA diagnostic screening requires substantial amounts of tumour and blood samples. However current screening methods face the challenges of limited DNA templates from minute retinal tumours and too much blood samples drawn from young patients. In addition, the starting DNA template amount and quality are important to ensure confident detection of disease-causing mutations. As the majority of RB1 mutations are unique and distributed throughout the RB1 gene with no real hot spots, the entire gene needs to be thoroughly analysed. This investigation proposes to enrich DNA samples using a whole genome amplification (WGA) step prior to RB1 mutation screening by RB1 gene-specific PCR amplification as well as high resolution melt (HRM) analysis and sequencing. It also identifies RB1 mutations in two RB patients and explores whether WGA and saliva products can be a source of DNA templates for RB1 analysis. In addition, this study was conducted based on the hypotheses that RB1 mutations were the underlying cause of the disease in the two patients, and that the products from WGA could be used specifically for RB1 gene analysis to overcome the constraint of insufficient DNA samples. Two anonymised genomic DNA samples from two unrelated RB patients and five normal healthy DNA samples were used in this project. WGA kits were compared according to three criteria, namely amplification yield, product fragment size and whether DNA is amplifiable. Prior to and after amplification, the optical density of two normal samples was measured to determine the increase in DNA yield. The amplicons were subjected to gel electrophoresis to determine the product fragment size. Exons 6, 14 and 25 of the original and amplified samples undergone PCR, and were examined again using gel electrophoresis to ascertain that the amplicons were amplifiable. Mutation analysis using HRM was carried out with pre-existing primers for all 27 exons and the promoter of RB1. Samples from patients were analysed against 83 saliva DNAs extracted using Oragene•DNA (OG-500) Kit. REPLI-g was observed to produce higher yield and products of reliable fragment size. Single distinct bands were also seen for exons amplified using REPLI-g, indicating that REPLI-g is more accurate and suitable in the amplification of DNA. Abnormal melt profiles were obtained for exon 6 in RB477 and exon 14 in RB572 for HRM. These exons were sequenced to determine the exact mutation. Exon 6 was found to have a splice-site mutation g.607+1G>T, while a point mutation, g.1363C>T (p.Arg455X) was identified in exon 14. Both the uses of saliva as a non-invasive DNA source and the WGA approach for enriching DNA sample for application in RB1 gene analysis have never been reported for RB. Although HRM analysis has been used for other diseases, this is its first instance applied in work on RB1 gene. In short, this report offers novel and promising approaches which would contribute significantly to the molecular analysis of mutations in RB.

本實驗主要闡明了三件事，1.如何去尋找在光學戲法上，其隱形區位置所在，並擺脫公式，從國中生就能懂得光學知識，來進行推論。2.解決仿照TheRochesterCloak不如預期的問題。3.捨棄TheRochesterCloak的作法，用我們自己的方法，打造一座隱形魚缸。 在1.這點上，當我們用平行於主軸的視線，平視透鏡時，物體若在接近焦點旁，其所成的像，會有部分落於，視野上的視覺不可見區域，以此為隱形理論基礎，透過不同透鏡組合及從視角、焦距、透視比例等方法，來達到使參考目標物所呈現的像，與原物為1:1比例且為正立，進而出現真正隱形效果。 最後，利用所有的推論和數據，我們使用自己的推算方式，來利用3D印表機，打造一台讓魚消失的隱形魚缸裝置。

給定一個有n個頂點的簡單圖 G，將頂點標號為1,2,…n；考慮 任意相鄰的兩頂點標號和中最大值的最小值，稱此極值發生時的標號為圖G的擁擠標號。在這個研究中，我們得出方格表、m×n×l長方體、環狀圖、圓柱圖及樹圖的擁擠標號和其極值的通式，並討論相關的問題。 Given a simplicial graph G of n vertices, label the vertices with 1,2,…n. Consider the minimum of the maximum of the sum of any two close vertices’ labeling, and we call the labeling that has this extremum the “crowded labeling”. In this study, we found out the “crowded labeling” and the common equation of the grid, m×n×l cuboid, cycle, cylinder, and trees. And discuss the correlative questions.

本研究主要探討地震造成的纜車晃動，藉由自製的地震模擬器與纜車系統，探討各因素對纜車晃動之影響，設計纜車系統和車廂的減震裝置。研究結果顯示：一、地震時纜車斷電停駛位置愈靠近塔柱、相同長度纜繩上車廂個數較多、纜車重量較重、纜車臂較短，纜車晃動時間相對較短。二、所設計之減震裝置中，利用夾層中鋼珠轉動，使地震時只有外層纜車晃動而內層纜車輕微滑動的「雙層纜車」效果最佳。纜車系統塔柱則可參照建築物設置隔震元件，以減緩纜車晃動。本研究結果可望為未來應用之參考。

我們在討論 John Mason 的 Thinking Mathematically 時，發現一個有趣的問題：「隨便寫下一個 0 和 l 的排列，若連續兩個相同的話， 在它們下面寫一個 0 ，否則寫一個 1 。重複這過程直到你只剩下一個阿拉伯數字數字。能預測最後一個是什麼嗎？」如： \r \r 此題看似簡單，但涉獵其中後，卻令我們沉迷於它的奧秘，進而想繼續探討。我們能預測最後一個數嗎？能擴展這個系統嗎？能知道 1 的個數嗎？

The researchers aim to construct an emergency mobile phone charging station that runs on renewable energy and will serve as a cost-efficient alternative to more traditional power banks. Circuit components include a 20V / 6W solar panel supplemented by a hand-crank gear mechanism integrated with a 6V / 1A lead-acid battery, a usb output and an adjustable switch-mode power supply (SMPS) to convert excess voltage into current. Initial voltage and current outputs were measured under varying resistances. It was determined that the set-up satisfied the minimum voltage and current requirement for charging a mobile phone (5V / 1A). A subsequent phone charging test was executed using a Samsung Galaxy J2 (3.85V Li-ion battery 7.70W, Charge Voltage: 4.4V / 2000mAh) wherein it charged on an average of 0.277% per minute for the solar panel and an average of 0.263% per minute for the hand crank gear mechanism. A Mann-Whitney U statistical test was conducted to determine if the charging rate of the charging station had a significant difference from a commercially available power bank’s. The calculated UA: (4) from the test was below the lower limit and the UB: (217) was above the upper limit which indicated that there was a significant difference between the charging rates. While the efficiency was lower than the commercial power bank’s, it can still be used as an alternative charging method especially during emergencies and disasters.

Parameters of traffic, road availability, and fare were integrated into a web-based application for determining the best public transport routes within Metro Manila in order to assist commuters in their travel planning, whether for business or for pleasure. A user-friendly interface was developed to obtain a user’s place of origin and destination, as well as preferences in travel time, mode of transportation, and cost of journey. By accessing the traffic roadway network of the metropolis, a real-time situation of road availability was obtained, and used in a modified Dijkstra’s shortest-path algorithm to produce a model of a real-time adaptive transport network of Metro Manila. From the model, an optimal route that considers the user’s preferences can be determined. This project will be immensely useful in helping both businessmen and tourists in planning their routes that will save on time and money.

Current medical intervention in cancer therapeutic methods has shown risks and side effects with normal tissues. This includes incomplete cancer eradication. In reference to numerous studies and literature reviews, a stimuli-responsive drug delivery system is selected as an innovative, safe and more assured treatment due to its site-specific release ability. This allows specific intervention upon the given stimulus which response to the presenting disease symptoms. Hence, we designed a ROS(Reactive Oxygen Species)-responsive BA-ADA(4-Hydroxyphenylboronic acid pinacol ester and 1-Adamantanecarboxylic acid bonded molecule) nanoparticle delivery system. In our study, ROS-responsive nanoparticle was designed and prepared based on a synthetic molecule from BA and ADA. A therapeutic payload, Doxorubicin, can be loaded into the nanoparticles and it can be selectively released within cancerous tissues whereby ROS level is over-expressed. This will enhance both therapeutic efficiency and reduce side effects. The stability and ROS-responsiveness of the particle were proven in a series of evidence-based experiments. The results showed a significant difference in cell viability during the experiments with healthy and cancerous cell samples. Further research will be required to extend the experiment in vivo.

When predicting a hurricane’s landfall location, small improvements in accuracy result in large savings of lives, property, and money. The project’s purpose was to apply a breakthrough method that can predict the geographic location of a hurricane’s landfall with high accuracy. Researchers have known for a long time that there are strong correlations between a hurricane’s landfall location and the geographic regions its track passes through. However, no methods have been developed to mathematically and explicitly describe these correlations. Consequently, the correlations can only serve to meteorologists as vague guidelines for their guestimates and are not usable in making practical forecasts. By studying the correlations and performing numerical optimization on historical hurricane data, this research discovered a set of geographic belt regions in the Gulf of Mexico that can be used as landfall location predictors. When a hurricane passes through any one of these belt lines, a prediction can be made by extending the hurricane’s moving direction vector towards land – the intersection point of this extension line with the coastline is the predicted landfall location. This prediction method is simple and straightforward. It only uses basic measurements from meteorological satellites: the hurricane’s real-time locations and moving directions. In conclusion, when compared to existing methods, the predictive belt method (PBM) created in this research provides a landfall location forecast with higher accuracy. Verification with historical hurricane data demonstrated that the PBM’s average error is less than 50% of the National Hurricane Center models’ error.

利用化學合成法合成出聚苯胺及 MEH-PPV，經過一連串的製程作出高分子發光二極體（PLED），再用 I-V 儀量測。實驗中以聚苯胺及 MEH-PPV 的薄膜厚度為變因，進行實驗。 電壓值為 10V，量測樣品通路上的電流。一開始電壓在跑時，量到的電流都為 0A，所得到的圖形為一水平直線；當電壓到達一個值時會向上爬升，但爬到一個階段後圖形呈現鉛直掉落至電流為 0A，圖形又恢復成水平直線。過程中看到了薄膜厚度對樣品確實有影響，又鋁電極的厚度不能承受過大的電壓，所以，用銦粒壓在元件上藉以保護。 Using Chemiccal polymerize PANI and MEH-PPV make through a series Polymer light emitting diode（PLED）Produce.then using I-V meter surveies. PANI and MEH-PPV change of thick proces.Experiment design I-V volt for meter conduction of current of sample .The voltage moving.First meter current is obtaining the figure is horizontal when voltage increase to a special value , but increase a while , the current will fall down to zero volt. Figure will go to horizontal that sample will change .Thick is different,and aluminum cathode can not suffer too much voltage .otherwise will burn.