Deficiencies in clearance of apoptotic cells predispose to the development of autoimmune disease. This is evident in mice lacking the receptor tyrosine kinases Tyro3, Axl, and Mer that mediate uptake of apoptotic cells. Deficient mice exhibit an increased abundance of apoptotic cells in tissues and manifest diverse autoimmune conditions. To test these mice for the presence of autoantibodies to apoptotic cells, we generated spontaneous splenic B cell hybridomas and used microscopy to screen for clones reactive with apoptotic Jurkat cells. From hybridomas secreting IgG antibodies reactive with apoptotic cells, we selected one that recreated the major serum specificity for apoptotic cells. The antibody, LHC7.15, bound to an antigen that is differentially distributed between the nucleus and the cytoplasm in live and apoptotic cells. In late apoptotic cells, the antigen coalesces into aggregates that form blebs at the cell surface. Immunopurification of the antigen, followed by mass spectrometry, identifed a protein of 69kD whose partial sequence matched hnRNP P2. This multi-functional protein binds DNA, RNA, and several known RNP autoantigens. Our observations suggest that an RNP complex, formed and translocated to the cell surface in apoptosis, participates in the induction of linked sets of anti-RNP autoantibodies. Our results also implicate hnRNP P2 as a potential novel antigen involved in initiating and sustaining systemic autoimmune diseases.

When one time we beautified our campus. It made us meet the snails, Euphaedusa sheridani shihi Chang, unexpectedly. Maybe snails make people associate with the holes on vegetables tops in thinking. Do all of the snails make vegetables tops as their food? We compared with the weight of the wood which has been stayed by snails or not. After a week, the weight of the wood which has been stayed by E. sheridani shihi Chang decreased obviously. It showed they also make wood as their food. What do they decompose wood become? First, we used the basic Carbohydrate's detection means to test the eluate of the wood which has been stayed by E. sheridani shihi Chang. However, we found both the eluate of the wood which has been stayed by E. sheridani shihi Chang or not can examine the Pentose out. So next, we plan to use SDS-PAGE to analyze the left enzyme on the wood, and use it to prove whether they secrete enzyme in mouth to decomposed wood or not. In addition, the holes these snails made and the environment are connected. By means of changing light, temperature and humidity to experiment with how much wood can these snails decompose. We found in the dark, about 20℃ and moist environment, they could decompose the wood the most. Finally, the holes they made on the wood also have its ecological niche. They decomposed the wood not only hastened the dissolution of the wood, but also provided the microhabitat for alga, fungi and small bugs.一次綠化校園的活動，製造了我們與石氏煙管蝸牛的邂逅。蝸牛，或許使很多人想到蔬菜上的洞，但真的所有的蝸牛都以蔬菜為食嗎？比對有無蝸牛棲息的兩組木頭，結果一星期之後有蝸牛棲息的木頭重量明顯減少，顯示蝸牛也以木頭為食。那麼牠們把木頭分解成什麼呢？我們先以基本的醣類檢測方法，檢測蝸牛棲息過的木頭表面洗出液，不過發現不管有無蝸牛棲息皆可檢驗出五碳醣，接著預計用SDS 膠體電泳看是否可以分離分析出蝸牛在消化木頭時殘留在木頭上的酵素，以證明蝸牛是否在口腔分泌酵素以分解木頭。至於環境和蝸牛消化木頭的關係，我們藉著改變光線、溫度、溼度等變因進行實驗，發現牠們在陰暗、潮濕、約20℃的環境下可以分解最多的木頭。最後，牠們分解木頭形成的洞也具有其生態意義，不僅加速木頭的分解，也製造了微棲地提供藻類、真菌及小型生物的生存空間。

As the most dominant species on planet Earth's surface, humans have become the sole protectors of the planet, in charge of keeping it hospitable for billions of species. However, a swift look around Egypt's streets in contemporary 2007 showed proof of our exploitive use of fossil-fuels. Provoked by such a scene, I set out to discover the negatives of fossil-fuel consumption that more than 2 million websites were ready to detail. The increased CO2 emission, due to fossil-fuel use, is contributing to the greenhouse-effect, causing global-warming and thus leading to climate-change. This affects biodiversity by causing the eventual extinction of species, thus upsetting food-chains and affecting humanity in the long-run. Statistically 40 more years of consumption are left if the current rate-of-consumption persists. My proposal is to use solar-energy as an alternative power-source. Two requirements must be fulfilled: it must hold research potential and shouldn't suffer from problems associated with fossilfuels. 3rd-generation nanocrystalline dye-sensitised solar-cells have a conductive-glass-electrode coated with titanium-dioxide, then a layer of dye on it for light-absorption, electrolyte and finally a counterelectrode coated with graphite.\r In the dye-experiments, dyes extracted from fruits & vegetables, and the wavelengths of visible-lightspectra that they absorb, were examined using a UV-spectrophotometer. The solvent for the extraction-process was tested and ethanol was better than water because it actively extracts the dye while water doesn't help and contains ions that alter the dye's absorption-abilities. I combined 2 dyes: chlorophyll (absorbs the red & violet areas in spectrum) with blackberry (absorbs the green area) thus enabling the solar-cell to absorb the entire spectrum and maximising its power-reading. I investigated whether the dyes should be freshly-mixed or combined earlier (and used every time to build the cell). Freshly-mixing dyes is better because that stops the enzymes that were extracted out with the chlorophyll from denaturing blackberry's anthocyanins and rendering them dysfunctional thus the dyes work better maximising the power. Testing the dye-combination's concentration showed that the 6.25% concentration is the best because it can coat the TiO2 with a single-layer of dye instead of high concentrations that give multiple layers of dye which doesn't give good electron transition or low ones that don't give enough dye which means a lower amount of electrons are being excited and thus less power. In the material-section, anatase and rutile, TiO2's 2 major constituents (structurally identical but with different crystal-orientation), were compared and anatase proved itself better because of its smoother conduction-band. Different anatase particle sizes were examined and the smaller particles (4 nm) were better because of increased surface-area. I investigated the ways of coating the counterelectrode and found that using carbon-paste is the best. Ultimately my solar-cell's power-reading increased from 2.04 nW to 6.41 μW which is 3142 times the initial reading, a dramatic increase for a solar-cell whose size doesn't exceed 4 cm2. This proved the great research potential held by solar-energy. After extensive comparisons between the ups and downs of both fossil-fuels and solar-energy, solar-energy clearly proved itself the perfect alternative for powering our future.

Based on Ampere，s Law, the magnetic field intensity of the solenoids is B=μ0μr?n?I, where μ0 is the magnetic permeability of free space, μr is the relative magnetic permeability, n is the number of coils per unit length and I is the solenoidal current. The end magnetic field of the solenoid must multiply by one half. According to the above result, it can be greatly strengthened by the addition of a ferromagnetic core. First, we observe three different inserted materials of coils (wood, iron and magnetite), whose magnetic induction in different solenoidial current. By experiment, when the iron and magnetite materials were inserted into the coil, it would produce larger magnetic induction. The calculated relative magnetic permeabilities of wood, iron and magnetite materials are 0.57, 18.37 and 18.32, which are close to the reported paper (1). When the driving field is removed, the fraction of the saturation magnetization of the magnetite is retained, which is called hysteresis and is related to the existence of magnetic domains in the material. In the second part, we change the frequency of circuit switch, which induced different current. Compared with the result of the first part, it would fit the result, which is the induced magnetic field is proportion to the solenoidal current. 根據安培定律，螺線管的磁場為B=μ0μr?n?I。其中μ0為真空中的導磁率，μr為相對的導磁率，n為單位長度的線圈匝數，I則為通入螺旋管的電流。至於螺旋管的端點磁場須再乘上1/2。所以根據上述的結果，當螺旋管插入鐵磁性物質，會增強螺旋管的磁場。首先，觀察三種不同的芯物質；非鐵磁性材料，軟磁材料，硬磁材料（木棒，低碳鋼棒，磁鐵棒）在不同的外加磁場下的感應磁場，得到芯物質的磁化曲線，而計算出來的相對導磁率分別為0.57, 18.37 和18.32與參考文獻(1)接近。而當外加磁場移走時，硬磁性物質的磁性仍然存在，稱為殘磁現象。在第二部分，我們改變線路開關的頻率。發現不同的開關頻率，會得到不同的螺旋管電流，而造成不同的感應磁場。再度驗證了感應磁場大小是正比於螺旋管電流的大小。

After short period of storage the coconut oil at room temperature we found that it becomes rancid. The oxidizing flavor is disgusting and causes economic loss. Some chemicals are used to prevent the oxidation of coconut oil but they are expensive and may be harmful to consumer’s health if used daily. Moreover, they are not suitable for homemade coconut oil and residual waste from the process may be environmental toxic. The present work thus aims at extracting substances from local plants and used as antioxidant for coconut oil. Seven kinds of local Caesalpiniaceae plants in Eastern Thailand namely Tamarind (Tamaridus indica L.), Peacock flower Caesalpinia pulcherrima L.), Flame tree (Delonix regia L.), Golden shower (Cassia fistula L.), Siamese cassia (C. siamea Lamk.), Candelabra bush (C. occidentalis L.), and Copper pod (Peltophorum pterocarpum DC.) were used. Ten grams of seed coats were mixed with 50 ml of the distilled water and 95% Ethyl alcohol (1:1) mixture in a closed container. The mixture was heated in a water-bath at 60 ℃ for about 2 hrs. and then kept in 10 ℃ icebox about 2 hrs. The mixture was then filtered and the filtrate was heated in an hot-air oven at 80 ℃ for about ? hrs. Ten ml of coconut oil was added with 0.5g of the extracts and kept at room temperature for 5 days. The pH and peroxide value (using the Iodometric titration method) of the oil were measured. It was found that the coconut oil with the Tamarind seed extract had the lowest peroxide value and was selected. Next the suitable amount of the Tamarind extract was studied by adding the extract to 10 ml coconut oil at 0-7% (w/v) and the contents were kept at room temperature for 5 days. The result showed that the 3% (and more over) Tamarind extract had the lowest peroxide value. Then the coconut oil with 3% Tamarind extract was kept at room temperature for 30 days. The pH and peroxide value were measured every 5 days. It was found that the oil could be stored for 25 days without significant change in pH and its peroxide value was less than 10 milliequivale n t oxygen per kilogram oil according to FAO/WHO (Codex) standard .Therefore, Tamarind seed coat at 3% could be used to retard the coconut oil oxidation for 25 days. This work presents the applicable use of plentiful local plants such as Tamarind seed, which is normally discarded, as an antioxidant for coconut oil.

-to prove my original hypothesis that colour affects the power that is produced by solar panels. \r \r - that the wavelength of an elctromagnetic wave affects the photon or quanta energy that the wave has.

在相對較高氧化電位下的前處理，這樣的活化步驟已被普遍接受。藉由這樣的活化步驟，廢煤渣（傳統鋼鐵業）轉變成能夠有效偵測微量鉛金屬離子的催化劑。微量鉛金屬離子的偵測是藉由方波剝除伏安法進行。在最佳化參數下，偵測鉛金屬離子的靈敏度為11.482μA/ppm(斜率??)，線性範圍為0.1－2ppm。最後，照光設備之應用亦可用來提升偵測鉛金屬離子時之靈敏度。最終實際應用則取天然的水進行實驗之驗證。The preactivation process (i.e., preanodization) at very positive potentials has been accepted as the prime activating procedure. By using the preactivation process, waste cinder (from steel industry) were converted into an efficient catalyst in the determination of Pb2+ in cinder-modified carbon paste electrodes. The possibility of determining Pb2+ at trace levels was examined by square-wave anodic stripping voltammetry. Under the optimized analytical conditions, the sensitivity, linearity, and detection limit are 11.482 μA/ppm, and0.1－2 ppm (r = 0.974). Finally, the lighting was also used to raise the sensitivity of the determining Pb2+. The practical applications were demonstrated to measure trace Pb2+ in natural waters.

新興的 BlockChain（區塊鏈）技術具有去中心化及資料無法被竄改的特性，在需要資料透明的領域非常適用，尤其 Fintech（金融科技）方面因貨幣貴重的特性而特別有優勢。慈善募款常常有用款不透明與部分用途不被大眾認可的問題；因此本研究使用BlockChain技術之Ethereum（以太坊）平台，成功設計出一套Smart Contract（智能合約），主要功能針對募款組織的所有撥款，須經捐款者投票，通過後才可撥用，將監督的權利歸還給捐款者，使慈善捐款能夠公開透明且受監督。同時也研究網頁前後端程式語言，設計一套大眾能簡便進行操作之介面，期望真正將此募捐平台推廣至社會。

The set consist two units; the first shall be at the school headmaster office, a box with some openings at the front in which the number of the class is written. In each opening there is lamp which lighting to show the teacher attending in the class, each class has a certain colored lamp.

這是一種可在膨脹狀態及未膨脹狀態間轉換的膨脹收縮瓶塞。本設計之瓶塞包含一彈性橡膠之塞座及一剛性塑膠之旋轉控座。該瓶塞在未膨脹狀態，可將瓶塞置於平口內將瓶塞順時針方向旋轉90度使瓶塞由未膨脹狀態轉換至膨脹狀態將瓶子密封；欲開瓶時將瓶塞逆時針方向旋轉約90度使瓶塞由膨脹狀態轉換至未膨脹狀態，可輕易將瓶塞從瓶子內拉出。根據顧客之需求設計瓶塞並選定適當之塑膠材料以製作旋轉控座及適當之衛生橡膠以製作塞座，依廠商提供塑膠及衛生橡膠之特性資料做有限元素分析預測橡膠元件受撐大之變形量，進行加工與製造印證分析之結果，與預期目標有相當的差異，故製作簡易之試件進行探求塞座內縮量與瓶塞膨脹量之關係， 探求瓶塞膨脹量與瓶子所能承受的壓力之關係，進而逆向設計瓶塞之塞座內縮量。 This is a kind of bottle plug that can change at the situation of swell or unswell.The design of this bottle plug includes a rubber plug and a rigid plastic controller that can revolve around. We can put the bottle plug at the top of the bottle and rotate it 90° c.w., the bottle pug will be at the situation of swell and then seal up the bottle. If we want to open the bottle, we just rotate 90° c.c.w., and the bottle plug will be at the situation of unswell and then we can pull the bottle plug out easily. I design this bottle plug according to the need of the customers; choose the certain plastic material to make the rigid plastic controller, and the properly rubber to make the plug; analyze and predict the amount of deformation by Finite Element Method in accordance with the characteristics of rubber and plastic supplied by the factories. However, the result and the expected result are quite different. In order to solve the problem, I make an easy sample to search for the relationship between the contraction of the rubber plug and the swells of the plastic controller and also the relationship between the swells of the plastic controller and the pressure that the bottle can endures. Then I design the contraction of the rubber plug on the base of the result of the experiment I made above.

本實驗中利用二氧化鈦能在常溫下經紫外線催化，分解空氣中的水分子，產生自由基的特性，攻擊幾丁聚醣中碳與氧鍵結的部分，使chitin 的分子量成功的從近50000 降解至3000以下；並可利用照射時間的不同，降解出分子量不同的chitin。此法不但大大排除利用化學法降解時廢液處理上的問題，而且還能利用照紫外光時間長短的不同來控制分子量的大小；又奈米級二氧化鈦(TiO?)在紫外光在短短四個小時之內就有很好的降解效果，除了節省了反應所需的時間外，降解前後幾丁聚醣的濃度也很高，因此所需的成本也遠低於當今利用酵素降解的方法。In the experiment, we used the properties of TiO? that can be catalyzed by UV rays and breaking the molecules of H?O and produce free radicals, which free radicals can attack the chemical bond between carbon and oxygen in chitin, successfully degrading chitin's molecular weight from 50000 to 3000.We also use different shining times to degrade chitosan into different molecular weight. In this way, we not only readily solve the problem of treating waste liquids produced by chemical degradation, but also control the molecular weight by different UV ray shining time. For another thing, TiO? in the nanometer level has excellent effect on degradation within 4 hours under UV ray shining. It not only cut back the reaction time but also produced high concentration of the chitin after degradation. As a result, the cost is much lower than that of using enzyme to degrade chitin.

本文中我們探討一個有趣的數列。這個數列有一個非常特殊的性質：將數列相鄰兩項的前項當分子，後項當分母，所產生的分數數列，恰好會出現所有的正有理數。 這個特殊的性質表示，可以將正有理數按照這個方式作排序，這個排序將完全不同於常見的正有理數排序的方法。 (1). 在正有理數的排序的結構中，我們做出許多有關於此數列的定理。 (2). 用數學歸納法證明此分數數列涵蓋所有正有理數，且每一正有理數只出現過一次。 (3). 將數列分割後，利用試算表製成數列規則表，並整理出快速的方法將數列表達出來。 (4). 將an 數列排成“樹＂的模式，可更快速的把正有理數寫下來。 (5). 最後，設計出搜尋正有理數的演算法，解決在分數數列中第n個正有理數會是多少；以及正有理數會出現在數列中第幾項的問題。 Let’s discuss an interesting sequence. There is a very special quality in it. In this sequence, choose two numbers, which are close to each other, and suppose the first number as “member” while the second one as “denominator.” Then we can get a fraction sequence that includes all of the positive rational numbers! According to this special quality, we can arrange positive rational numbers by the following method. Then we can get a brand-new way of the arrangements. (1). We can find many theorems about this sequence according to this special arrangement of the positive rational numbers. (2). We can prove the rule that this fraction sequence includes all of the positive rational numbers by mathematical induction. Furthermore, every positive rational number appears only once. (3). After dividing this sequence into several parts, we can get a sequence rule list by using trial balance and find a faster method to express the sequence. (4). Arrange the an sequence by the tree model. By this way, we can get all of the positive rational numbers much faster. (5). Finally, we can develop the operation method to solve the questions that what position would one positive rational number be in the sequence and what is the first, second, third or nth positive rational number of the sequence.