The experiment was primarily focused on studying whether the enzymes from different bacterial species collected in various hot spring areas still exhibited activities at high temperatures. A further study would be conducted on analyzing the unique characteristics universally found in the genes of selected bacteria. First, hot spring samples were collected form Peitou and Wulai, and then cultured on the PY, PTG, MFB, and TS media in the laboratory. After the broth media growing with thermophilic bacteria, a series of continuous dilution method and solid-plate spreading were applied to separate these bacterial clones. The genomic DNA of the selected bacteria was extracted and used to analyze subtilisin-like gene by polymerase chain reaction (PCR) and electrophoresis. Finally, we examined the six selected thermophilic bacteria with the enzymatic activities of fibrin- and milk-protein degradation. We successfully concluded the experiment by proving that these thermophilic bacteria still exhibited significant enzymatic activitives in the high-temperature environments. The results of this experiment can be applied in numerous fields, for example, thrombus treatment and food processing, and a more in-depth study shall warrant the due consideration. 這次的實驗，主要是研究在不同的溫泉區中所採集不同種類細菌，是否酵素在高溫下仍具活性，如果有，再進而研究它們的基因有何特別的共同之處。首先，我分別自北投和烏來採集水樣，到實驗室後再以PY、PTG、MFB 和TS 四種培養基做細菌的培養，接著再利用連續稀釋和固態塗抹來做細菌的分離。經過挑選和培養之高溫菌直接進行DNA 的抽取，並利用「聚合?鏈反應」和「電泳跑膠」技術分析其類似蛋白質分解酵素subtilisin 基因。另外，本研究同時針對所選定之6 株高溫細菌利用血栓和牛奶蛋白來測試其蛋白質分解酵素的活性。由以上實驗結果可以證明某些細菌在高溫的環境下酵素仍具活性。這次在高溫菌的實驗結論，可應用在很多地方，例如：血栓的治療、在高溫下食品處理‧‧‧等，應用相當廣泛。

NO.57-08 2018 OCT | 科學研習期刊目錄 本期專題 溫柔的力量：提升女性科學「實作」能力 溫柔的力量：提升女性科學「實作」能力 | 陳鴻宜教授、蘇萬生博士 跟女同學談科學是什麼 | 陳育霖教授 趣味科學實作課程的溫柔體驗 | 葛士瑋博士 自造你的科學實驗 | 賴雲立老師 女生愛科學 | 謝迺岳老師 如何提升高中女學生的科學學習興趣 | 于曉平教授 森棚教官的數學題 用三湊三 | 游森棚 科學讀書會 玩出創意5：50個魔法科學實作 | 白榮銓 教學現場 STEAM之小學科技教學活動設計與實施-以抖抖外星人為例 | 張玉山、李姿儀 總召集人的話 促進女性對科學(乃至STEM-科學、科技、工程與數學)的參與是因應全球挑戰(如環境污染、氣候變遷)的關鍵之一。例如，聯合國教科文組織於2017年出版的報告《破解密碼：婦女的STEM教育》(Cracking the code: Girls’ and women’s education in science, technology, engineering and mathematics (STEM))即旨在破解妨礙或促進婦女在STEM教育參與、成就和延續的因子，並指陳教育部門可以做些什麼，以促進婦女對STEM的興趣和參與。女性參與科學(或STEM)也是我國的重要教育課題之一，本期特別以「溫柔的力量提升女性科學『實作』能力」為專題，請臺灣師大物理系副教授陳鴻宜博士和科教館編輯蘇萬生博士擔任特約主編。 專題文章含專題導言共有六篇：「專題導言」〈溫柔的力量提升女性科學『實作』能力〉指出女性能否進入科學領域，最重要的因素之一或許在於是否有人及時鼓勵，並簡介其它五篇專題文章的來源。〈跟女同學談科學是什麼〉一文從「科學是什麼」和「科學存在的目的」談起，再以日常生活的實際物理實驗演示要求學生現場進行科學探究，立即與同組同學討論如何設計一套實驗方法來向他人證明自己的論點。〈趣味科學實作課程的溫柔體驗〉一文透過有趣、可動手參與及親身探究的DIY科學與實作體驗學習過程，讓女性在學習物理的過程中，展現出精巧的動手作能力、運用科技工具完成電路組裝，驗證物理知識，並體會物理之美與世界的連結。〈自造你的科學實驗〉一文分享利用容易取得或製作的平價器材自製光譜儀和數位溫度計的一些想法與設計。〈女生愛科學〉一文從兩性學科學的基本差異、合作分工、相互欣賞和趨利避害四個面向，分享其看法。〈如何提升高中女學生的科學學習興趣〉一文則先論述科學議題中的性別差異以及女性科學生涯的困境，再建議五種有助於提升女生科學學習興趣之方式與活動。 「森棚教官的數學題」刊登〈用三湊三〉一文，拋出以3的次方數(1, 3, 9, 27, 81…)用加法湊出3之倍數的問題，供讀者自主探索或發想科展題目。 十二年國教自然科學課綱期望強化學生的「科學探究能力」，但是究竟在科學實作中要如何玩出創意以及科學探究要如何融入科學實作？本期「科學讀書會」導讀《玩出創意5：50個魔法科學實作》一書，該書內含七大類共50項的實作科學實驗。各單元包含：操作過程、原理說明、叮嚀的話以及參考資料。 「教學現場」單元刊登〈STEAM之小學科技教學活動設計與實施-以抖抖外星人為例〉一文，分享一個國小STEAM教學活動--抖抖外星人，這樣的活動除了結合STEM + Arts的知識運用，也要培養學生動手實作的習慣。 總召編輯委員 - 李隆盛 關於本刊 出版單位：國立臺灣科學教育館 發行人：陳雪玉 總召集人：李隆盛 編輯委員：古建國/全中平/李文獻/李旺龍/李源順/李進寶/林沛練/林坤誼/許良榮/蔣中柱/胡秀芳/陳正改/陳竹亭/陳玫良/楊水平/游森棚/謝隆欽/張子超 策劃：曾聰邦 主編：錢康偉 本月專題特約主編：陳鴻宜/蘇萬生 編輯：吳郡怡 網頁設計編輯：施曉恬/陳璽君 投稿規範請來信詢問：article@mail.ntsec.gov.tw

An Automatic Fine system for the handy-caps parking spaces We human beings Are developing creatures, And we believe that the Importance of scientific innovations depends on how much can they contribute in humanities services. Me and my friend worked so hard to present an Invention or a system that is going to make people lives better. In this point of view that we humans believe in. We work hard and we present Inventions, science fears, and new Ideas by a purpose and an intention that those thoughts, Inventions, Ideas, researches …etc. Will make us better people And will help in building a brighter future for mankind. Invention identity Name of the invention: My parking space,, My Right !! Components: Ultrasonic sensor, color sensor, RFID sensor and reader, buzzer, lights, NXT robot, conductive means (wires) How does the invention work? Operating Process The first point we want to make it clear to you that we have two stages: now (present) and later in real life. For the moment : We are using an educational robot (NXT mindstorm) with a programme from our design , using the Ultrasonic sensor to know if there is a car parking or not then using a color sensor to determine if the car is allowed to park or not And if not then write a ticket and a fine but before that it gives an alarm to notice the driver. The main objective of the invention We want to help maintaining the lost rights for the handy-cap people in their parking spots. Because we gave them less than what the numbers say we should of give them so we didn't give them what they deserve and we came at the same time and steeled it from them. this invention is used: usage fields This invention will be used in the handy-caps parking spaces as well as they will help of the economic. It can be employed and used instead of a lot of security persons or traffic Police department. The future vision of the invention It can be combined in a one small unit and with touch panels to know if there is a car parking or not, RFID to determine if the car is allowed or not to park in this space and a camera to know the exact car or maybe by reading the electronic chip in the cars plate .

國立自然科學博物館推出《數位博物館》，內含大量生物圖鑑及豐富館藏文物之圖文資 料，無論用於資料查詢或提供進階研究資料皆有極大貢獻。但經使用後發現，其檢索架構仍 只提供關鍵字搜尋及分類瀏覽，無法精確檢索出欲查詢之資料，尚有改進之處。 於是針對現有之影像檢索系統，我們跳脫出傳統以文字為檢索之依據，而設計出一連串 之改進方案，如下： 1. IRHI 色調辨識影像檢索，針對色調相近之影像類群提供良好檢索架構。 2. IRCI 輪廓辨識影像檢索，針對輪廓相近之影像類群提供良好檢索架構。 3. IRHCI 色調暨輪廓辨識影像檢索，綜合前述兩種方法之優點所設計。 A while ago, the National Museum of Natural Science put forth the “Digital Museum,” which contains great amount of biological pictographs and abundant collections of textual as well as pictorial materials. This has contributed tremendously to information search and advanced research. However, after employing them, we come to discover that its retrieval paradigm only provides key-word search and categorization browse, without enabling us to precisely pick out the desired data. Thus, this paradigm leaves something to be desired. To make up for the insufficiency of the existing system, we have escaped from the concept of searching by texts. Instead, we have designed a series of improvements. They are as follows: 1. IRHI(Image Retrieval by Hue Identification): Providing a sound paradigm for the image groups composed of similar hues. 2. IRCI(Image Retrieval by Contour Identification): Providing a sound paradigm for image groups composed for similar contours. 3. IRHCI(Image Retrieval by Hue and Contour Identification): Combining the strengths of the above two paradigms.

何謂無限解？若n位正整數B ，滿足xm xp mod 10n，則稱B 是xm＝xp的n位數解。例:2494＝3844124001、2492＝62001，所以249為x4＝x2的一個三位數解。當n→∞時，則稱B 是xm＝xp的一個無限位數解，簡稱為無限解。本研究主要在探討方程式x2＝x、x3＝x、x4＝x、、、、xm＝x、xm＝xp無限解的規律性及各方程式無限解的個數，並加以證明。幸運地，我們利用任意數次方後其個位數每四個循環一次的特性，藉由整數論定理的推導，並佐以Mathematica軟體輔助計算，得到以下令人振奮的結論！在Xm＝Xp的無限解中：(1)若m－p＋1為4k-2或4k(即m－p＋1為偶數)k N，則其必僅有兩個無限解。(2)若m－p＋1為4k-1，k N，則其必僅有七個無限解。(3)若m－p＋1為4k+1，k N，則其必僅有十三個無限解。

本文將一道組合問題可能的方法數製成正方形表格，以「巴斯卡正方形」命名。將此正方形內的組合數同餘若干自然數後，觀察及歸納其結果。 同餘後不同的餘數配予不同顏色，產生一些特別的圖形，例如：「教大中庭系列」及「棋盤格系列」。我們研究了這些圖形的規律性以及對各個餘數的個數進行計數。 我們利用Lucas’s Theorem及(1+x)n 展開的係數搭配乘法原理來做計數的工具，並且研發了一個算式用於計算任意組合數被質數的次方同餘後的結果。

本實驗在微小的測試環境下，以熱線風速計偵測壓縮空氣流經圓柱鈍體後方渦旋逸放的頻率，推導出雷諾數(Re) 與史卓荷數(St) 之關係。預期能利用有效雷諾數的概念，探討加熱圓柱流場的Re-St 關係，將25℃、50℃、100℃、150℃、200℃時的臨界雷諾數回歸成有效臨界雷諾數，導出Re 之分母---空氣黏度，以後便能以此不同環境溫度所對應之空氣黏度方程式，於各式環境下量得空氣的黏度。但因為在實驗中碰到了量測精度的限制，所以這個部份只有做現象的探討，並由觀測渦流逸放頻率發現到加熱圓柱確實可有效地穩定流場。未來也將會提高量測的精度，以期望能達到辨識氣體的效果。;The purpose of this experiment is to measure the vortex-shedding frequency while the compressed air flow over a cylinder by hot-wire anemometer and all of the experiment is set up at the small testing environment. By this way, we can find out the relationship of Reynolds number and Strouhal number. We expect that research the relationship between Re and St while flow over a heated cylinder by using the concept of effective Reynolds number. We can get the effective Re by curving fitting the critical Re at 25℃、50℃、100℃、150℃、200℃each and derive out the viscosity of the air. After this, then we can measure the viscosity of the air everywhere after knowing the viscosity with respect to the specific temperature. We only discuss the phenomena at this part, because the limitation of the accuracy of the instrument. We also observe that a heated cylinder can stabilize the flow field effectively by the vortex-shedding frequency. We are going to enhance the accuracy of the instrument and fulfill the gas identification.

碘化銅（CuI）為一種P型半導體，在一般文獻中大部分被用做催化劑，合成極為不易。我們利用一簡單電化學合成的方式，可將銅控制在一價銅( Cu?O )，如在有碘離子( I－ )的溶液中便形成CuI。在這研究中我們將所合成出之P 型半導體CuI 製成光感測器，在偵測不同光強時有良好的線性關係(r2=0.9961)。在光感測器的實驗中我們利用CuI 電極，讓它接受光照，使其電流產生光電流，如果能儲存其光電流能量，就可成為一太陽能電池，利用其原理，未來可以發展成為替代能源。 CuI is a kind of P type of semiconductor in the general literatures. Most of the CuI is use to catalyst. Except that, CuI is difficult to synthesis. We use a simple way of Electoctrochemistry complex to keep Cu?O under controlling. For example, in the I- solution, Cu becomes CuI. In the study, we make the synthesis P type of semiconductor to be light sensor. In different lightness, the procedure showed good linearity(r2=0.9961) In the light sensor, we use CuI pole to accept sunlight. Then the current will produce light current. If we could store the energy of light current, it will be a solar energy battery. When we apply the theory, it can develop to be substitute energy.\r

During the biology classes from junior to senior high, we have learned many interesting instance of different animal behavior. Most people paid more attention on the Vertebrates as their experimental subjects. The other species around us, although with simple body structures, may behave rather complicated and versatile reactions. In particular, one of the most common insects with simple body structure in our neighborhood is the American cockroach (Periplaneta americana). The aim of this study is to investigate the different swing motion modes of antennae of American cockroach by computer-aided Imaging Analysis. The parameters of each swing motion mode were calculated in order to analyze how light (including light stimulation and light adaptation) and vibration may affect the antennae behavior of American cockroach. It was found that the antennae swing motion modes were significantly different under different types of stimulus. If two types of stimulus occurred at the same time, the reactions of antennae motion may become conformable. In conclusion, antennae behavior has shown to significantly affect the survivability and environmental adaptation of American cockroach. Not only the antennae are considered as the sensitive receivers; but also they are the important transmitters to reflect physiological status and environmental condition.從國中到高中的生物課堂上，我們學到許多有趣的動物行為例子，但前人多以脊椎動物作為研究對象，而我們身旁的許多生物，身體構造雖較為簡單，但行為表現卻豐富多樣，尤其是常見的美洲蟑螂(Periplaneta americana)，可說是最親近我們的昆蟲之一。本研究以攝影紀錄的方式，透過電腦進行影像分析，記錄不同刺激下蟑螂的觸角擺動模式，並計算出各項觸角運動的參數，以瞭解光線(照光刺激或照光適應)與震動刺激對其觸角行為的影響。我們發現在不同因子的刺激下，觸角擺動的模式具有差異，若兩種刺激同時發生，蟑螂觸角的行為亦具有整合性的反應，證明蟑螂觸角的行為模式，對其生存與適應具有重要意義。這也代表觸角除了為敏感的受器，亦為能反映出生理與環境狀態的重要動器。

When one time we beautified our campus. It made us meet the snails, Euphaedusa sheridani shihi Chang, unexpectedly. Maybe snails make people associate with the holes on vegetables tops in thinking. Do all of the snails make vegetables tops as their food? We compared with the weight of the wood which has been stayed by snails or not. After a week, the weight of the wood which has been stayed by E. sheridani shihi Chang decreased obviously. It showed they also make wood as their food. What do they decompose wood become? First, we used the basic Carbohydrate's detection means to test the eluate of the wood which has been stayed by E. sheridani shihi Chang. However, we found both the eluate of the wood which has been stayed by E. sheridani shihi Chang or not can examine the Pentose out. So next, we plan to use SDS-PAGE to analyze the left enzyme on the wood, and use it to prove whether they secrete enzyme in mouth to decomposed wood or not. In addition, the holes these snails made and the environment are connected. By means of changing light, temperature and humidity to experiment with how much wood can these snails decompose. We found in the dark, about 20℃ and moist environment, they could decompose the wood the most. Finally, the holes they made on the wood also have its ecological niche. They decomposed the wood not only hastened the dissolution of the wood, but also provided the microhabitat for alga, fungi and small bugs.一次綠化校園的活動，製造了我們與石氏煙管蝸牛的邂逅。蝸牛，或許使很多人想到蔬菜上的洞，但真的所有的蝸牛都以蔬菜為食嗎？比對有無蝸牛棲息的兩組木頭，結果一星期之後有蝸牛棲息的木頭重量明顯減少，顯示蝸牛也以木頭為食。那麼牠們把木頭分解成什麼呢？我們先以基本的醣類檢測方法，檢測蝸牛棲息過的木頭表面洗出液，不過發現不管有無蝸牛棲息皆可檢驗出五碳醣，接著預計用SDS 膠體電泳看是否可以分離分析出蝸牛在消化木頭時殘留在木頭上的酵素，以證明蝸牛是否在口腔分泌酵素以分解木頭。至於環境和蝸牛消化木頭的關係，我們藉著改變光線、溫度、溼度等變因進行實驗，發現牠們在陰暗、潮濕、約20℃的環境下可以分解最多的木頭。最後，牠們分解木頭形成的洞也具有其生態意義，不僅加速木頭的分解，也製造了微棲地提供藻類、真菌及小型生物的生存空間。

During the evolution of humankind, development of frontal cortex has played a critical role, where higher brain function like emotions, self-consciousness, decisions…etc, were all related to frontal cortex. On the other hand, thalamus is usually associated with relaying the sensory signals from peripheral receptors. In order to understand the functional role of frontal cortex, the signal processing mechanisms in the thalamo-frontal cortical pathway became an important research issue. The aim of this experiment was to find a method to dissect a brain slice that contains a connecting route in vitro between thalamus and frontal cortex with functional activity. Through nerve fibers tracking technique using fluorescent-dye (DiI), it was understood that the 3D-space connection between thalamus and frontal surface was an upward curve with a turn of about 110 degrees and bending inwards from the two sides. If a conventional horizontal section was performed, the route would be cut-off and its integrity lost regardless of the direction. To solve this problem, a novel section method was developed to retain the route. Based on the route direction shown by the fluorescent-dye, a piece of brain block was cut and flattened of about 110 degrees. Other sections were performed as control for studying the effectiveness of the sectioning on the plane of the route. Finally, electrophysiological methods were used to verify the connection route was complete and functional. Thalamus-evoked extracellular field potentials in the frontal cortex were observed by changing stimulation strength, adjusting slice temperature and prepared oxygen supply and administration of drugs like CNQX and picrotoxin in the 110 degree flattened slice but not the others. It was found that the reaction was essentially a neuronal response, indicating the pathway between thalamus and frontal surface was retained substantially. With this novel brain slice technique, we can assess the functional connection between thalamus and frontal cortex and investigate the cellular mechanisms of the signal processing in this connection pathway. It is anticipated that present technique provides an important step to further elucidate the functional role of the frontal cortex. 在人類的演化史上，前額葉的發展扮演了極為重要的角色，凡舉情緒、自我意識、決策等，皆與前額葉有關。而丘腦通常與視覺、聽覺及本體感受如痛覺、觸覺、溫度覺的訊息傳遞有關。要了解前額葉的功能，丘腦到前額葉的徑路及訊息處理機制，便成為一個很重要的研究課題。本實驗的目的是尋找一個方法能在離體的腦切片上維持具有丘腦到前額葉連結的徑路並且有功能的活性。經由螢光染料(DiI)神經束追蹤技術，了解從丘腦至前額葉路徑的三度空間連結為一先向下再向上約110度的角度轉折，並同時先由內向外再轉向內的曲線，若用一般水平之切片方法，無論何種方向，其路徑必定會被切斷，不能保持其完整性。為了解決此問題，發展出一種可以保存其路徑的腦切片方法。依照螢光染料所顯示出的的路徑走向，在腦塊的皮質上切一刻痕，將腦塊以110度的角度展平，使其路徑處在一個平面上再切片。最後利用電生理的方法來證明所切出的連結路徑是完整且具有活性。改變在丘腦的刺激強度、調整腦切片(腦脊髓液)溫度、氧氣的供給以及施予藥物CNQX、picrotoxin，觀察其前額葉之電位變化，發現其反應確實為神經反應，表示從丘腦至前額葉的路徑已在這種特殊的腦切片中被完整保存。藉由這個方法，將有助於研究丘腦至前額葉功能性連結，神經網路結構，及其訊息處理機制，並期待以這樣一個全新的方法將來有助於瞭解前額葉的功能。

Up to this time we have spent almost three years in studying condensation and water droplets. Little could we have done as compared with the almighty nature. However we are rewarded by the nature as we gradually found the secrets about electro-magneto field and water droplets: The size of water droplets turn smaller upon electro-magneto field and grow more uniformly especially upon electric field. This experiment presented here is actually the diary of the growth of water droplets in condensation, upon magnetic field and electric field. Through convection, it discusses the self assembly patterns of water droplets and peep into the uniformity both of the size and the distribution mode of water droplets. In former basic experiment, we focus on temperature and the speed of water moisture; generally speaking, higher temperature speeds up the coalescence procedure but does not affects the nucleation size of water droplets in simple plain surroundings; while speed of moisture does affects the nucleation size. As we went farther, deep into convection and found magneto-electric force did play an important role in the self assembly mechanism of water droplets. The topic is mostly concerned as we are surrounded by magneto-electric waves in today’s world. This experiment anchors the first step in discovering the uniformity of water droplets in different environment, and providing insights into the self assembly mechanism of water droplets upon electro-magneto field with nano sizes. 這是一系列關於水蒸氣冷凝為極細微小水珠的實驗。其中可以分為兩大部分； 第一部分是基礎實驗。將水蒸氣導入至潔淨的介面上(蓋玻片)，觀察冷凝水珠的結構。雖然看似簡單平常，但卻有令人驚奇的發現；不同溫度的水蒸氣，其冷凝最初始的細微顆粒之尺寸是相同的 ！爾後隨著溫度的升高，堆疊速率也跟著上升；以致於最後一起呈現出來的水珠大小不一，尺寸不一。 第二部分是將水蒸氣導到磁場及靜電場上，觀察其冷凝結構。這部分的實驗推翻了一般「水分子是電中性在電磁場中不受影響？」的刻板觀念 ！實驗所呈現出來的冷凝水珠，不但於附加磁場中尺寸縮小又不易長大，同時還有固定的自我組成模式( Slef-assembly pattern);而且也發現在磁場中的冷凝小水珠的尺寸比電場中的小，可是電場中的小水珠則表現出較大的均勻特質。