本實驗是以實際探勘的地質及泉水資料和文獻為基礎，對於幾個可能造成冷泉降溫的原因(岩石種類、泉水pH值、流通氣體之壓力、流速)進行實驗。我們製作了一個模擬地下泉水流動的實驗管路，此管路並可同時觀察紀錄氣壓、氣體流速，以及實驗前後模擬管路的溫度變化。透過對實驗管路的熱容量校正，我們可以找出各變因對泉水降溫的關係，以建立出一個模型，期望可套用於實際冷泉的狀況，進而推論出更多冷泉的性質。This experiment uses data and bibliography from real exploration as bases to find the possibilities of why the cool spring drops in temperature. (Mineral types, spring water's pH value, air flow pressure, and movement flow.) We created a model of underground spring water for the simulation. From this model, we could observe the air pressure, movement, and the spring's change of temperature before and after the tests. By adjusting the thermal capacity in the model, we could find the direct cause of the spring's temperature drop. And hopefully be able to adopt this model to the realistic problem, increase the effort of analyzing the natural cool spring's properties.

此項研究是用溶氧計連續偵測培養液中之溶氧量，藉以分析固氮\r 單胞藻RF-1 的光合韻律。實驗的結果清楚地看出RF-1 之光合韻律，\r 而且能夠清晰地觀察到其中光合作用、呼吸作用和固氮作用之間的相\r 互關係。研究中還發現：在不進行光合作用時，RF-1 的固氮作用也\r 會停止；另一方面，在有進行光合作用、但是停止固氮作用的狀況下，\r RF-1 光合作用之韻律現象會喪失。這些實驗結果值得未來更深入地\r 探討。報告中也包括了單胞藻PCC6803 的光合韻律實驗，藉以做為\r 有固氮能力與沒有固氮能力藍綠藻之間的對照。The variation of dissolved oxygen (DO) in the culture of the\r unicellular nitrogen-fixing cyanobacterium Synechococcus RF-1\r under diurnal light/dark condition was detected automatically by DO\r meter. The results indicated that the algae exhibited circadian\r photosynthetic rhythm. The results also revealed that a respiration\r rhythm occurred at dark phase when the culture was grown in\r nitrogen-free medium. There was correlation between the rhythm of\r photosynthesis and nitrogen fixation. The nitrogen-fixing activity\r could not be detected if the photosynthesis was stop by cultivated\r under continuous darkness. On the other hand, the rhythm of\r photosynthesis could not persist when the nitrogen-fixing activity\r was inhibited by NaNO3. In order to compare the difference\r between Synechococcus RF-1 and the cyanobacterium, which\r cannot fix nitrogen, the DO pattern of Synechocystis PCC6803 was\r also investigated.

DcR3是腫瘤壞死因子6B的成員（Tumor necrosis factor receptor superfamily），又稱為TR6或M68。DcR3會藉由與Fas Ligand, LIGHT或TL1A等細胞表面配位結合，阻擋T細胞的死亡訊息傳輸，保護該細胞免於細胞凋亡；另外，DcR3也會與抗原呈現細胞的硫化肝素糖蛋白結合，誘導未分化之T細胞發育成能減少自體免疫疾病發生的Th2細胞，顯示DcR3有充分作為自體免疫疾病預防之藥物的可能性。因此我藉本實驗探討DcR3對T細胞分化與發育造成的影響與造成此一現象的原因。 根據實驗結果，我們發現DcR3不僅有上述之功能，更能增加調節型T細胞（Treg）這種抑制免疫反應的特殊T細胞表現量，若能藉由Treg調控免疫系統活力，將可改善易般免疫抑制劑治療造成病人免疫力下降之缺點。因此我藉由NOD小鼠，實際檢測DcR3在活體中對抗自體免疫疾病發生的能力，並以DcR3與T 細胞之交互作用實驗，探討現象的成因。根據實驗結果，在Somatic gene therapy和Protein therapy的實驗中，皆可看出疾病延遲發生的現象。而在DcR3對T細胞存活率的影響方面，可看出DcR3並不會造成T細胞之異常死亡。故我們推測DcR3可藉由增加Treg表現量減緩或降低NOD小鼠糖尿病的發病。 因此我們認為DcR3非常有潛力作為未來自體免疫疾病預防或移植手術後用以抗排斥之藥物。

超疏水表面(superhydrophobic surface)因其自我清潔效果在近年來引起廣泛的研究及探討，目前世界各大公司皆積極投入自潔性產品(超疏水表面)的研發，但是截至目前為止除了塗料及織物有產品外，其他尚在研發階段。降低表面能及增加表面粗糙度為製作超疏水性表面的兩種方法。本實驗我們針對使用不同方法去仿照自然界植物「蓮花效應」，將二氧化矽粒子適當的分佈在基材表面，以增加粗糙度，製造出超疏水性表面。我們發現鍛燒會使其二氧化矽粒子黏聚在一起，對接觸角的增加無幫助。而在溶膠凝膠的配置過程中直接加入OTS(Octadecyltrichlorosilane)，並利用光散射儀比較二氧化矽奈米顆粒的平均粒徑及粒徑分佈隨時間變化的情形，一段時間後發現會有明顯大顆粒沉澱，塗佈在玻璃基材表面後容易脫落，不適合往後實驗或其他用途。而以氨水催化的溶膠凝膠以不同流速滴入TEOS (Tetraethylorthosilicate，四乙氧基矽)對接觸角的影響也不大。最後我們利用砂紙在基材表面上刷磨，可以使二氧化矽粒子分佈均勻，明顯增加接觸角。塗佈速率的改變搭配疏水性矽烷單分子膜的改質，我們已可以製造出159°的超疏水性表面。Superhydrophobic surfaces are generally made by lowering the surface energy and increasing the surface roughness. In this experiment, we use different methods of spreading silicon dioxide nanoparticles properly on the surface in order to increase the surface roughness and also make superhydrophobic surfaces. In the beginning, we find that the calcinations can cause its silicon dioxide nanoparticles to stick together instead of increasing the contact angles. Then, add OTS (Octadecyltrichlorosilane) directly to manufacture process of sol-gel, and observe the situations of the average length and the spread of silicon dioxide nanoparticles with the time goes by. After a period of time, we will discover that many obvious big particles deposit and spin-coating on the glass surfaces flop easily. Hence, this phenomenon is n’t proper for the following experiments or other uses. However, the contact angles have nothing to do with dropping the sol-gel catalyzed ammonia to TEOS(Tetraethylorthosilicate) by different flowing rates. Finally, it is crucial for us to use the sandpapers to brush on the surfaces because it may cause the silicon dioxide nanoparticles to spread well and obviously increase the contact angles. Combining the silicon dioxide surfaces with the change of spin-coating rate and the cover with hydrophobic SAM, we have made the superhydrophobic surfaces of 159°.

茶由於其具有特殊的芳香氣味及口味，廣泛地被世人用為飲料，近年來的研究證據顯示茶具有促進身體健康和防癌功效，但茶中之成分，尤其兒茶素隨茶及茶沖泡方式而有所不同，因此對於茶中成分及沖泡方式的認知是一門重要的課題。 本實驗主要是探討茶沖泡方式及儲藏條件，對於茶液中成分變化的影響，探討的茶包含紅茶、烏龍茶、綠茶及其茶包。並使用質譜儀及高效能液相層析儀配備紫外光偵測器分析茶液中之成分，實驗結果顯示加蓋子的茶杯儲存茶時比沒蓋子的要好；成分變化速率又以未發酵的綠茶較發酵的紅茶和烏龍茶快；低溫儲存時，亦可延緩茶的成分變化，例如加蓋並存放於冰箱，茶甚至可置放至隔夜其成分均不會改變；尤其本實驗尚發現照光與否影響茶液成分變化甚鉅。;Tea is the most widely accepted and consumed beverage worldwide due to its characteristic aroma and taste. Recent studies have provided the strong scientific basis for understanding the health promoting effects and cancer preventive actions of tea. The components of tea especial the catechins are varied with the conditions of making tea. To understand and determine the chemical composition of tea is very important. Some investigations of the parameters on the storage and making of tea were carried on in this study. The kinds of tea studied were including black tea, oolong tea, green tea, and instant tea bags. A high performance liquid chromatograph combined with UV detector was used to analyze the components of tea. The results showed that the composition of tea solution is dependant of the exposure of light. The tea kept in cup with cover was better than that without cover. The ingredients in green tea were changed faster than those in fermentative black tea and oolong tea. The components of tea can be kept unchanged for a long time at low temperature. In refrigerator, the time can be extended to overnight.

The purpose of this paper is to study the roles of formic acid and formaldehyde playing in the Direct Methanol Fuel Cells(DMFCs).The assumption is now widely accepted that the Oxidation-reduction intermediate of cell like formic acid and formaldehyde will hinder the reaction of DMFCs. At first, we recorded data which measured the efficiency of DMFCs working under the different temperature of fuel, then we recorded data which measured the efficiency of DMFCs working under the different consistency. In the end, we compared the data we recorded before and chose the best reaction environment as standard environment for the future experiment. Then we discover intermediate has positive effect on DMFCs. we separately put formic acid and formaldehyde into fuel, and we discover the efficiency is better than\r before. For example, the volt stability and volt intensity of DMFCs are better. The above conclusion is mainly based on open current volt, equally volt and electric current density which is the standard of evaluation.本實驗主要探討甲酸、甲醛等雜質在直接甲醇燃料電池中扮演的角色，一般認為甲酸等是甲醇在電池中反應的中間產物，大多數人認為這些中間產物會阻礙燃料電池的反應。而我們先針對了甲醇在各種溫度下電池的效能先做出了圖表，並使用不同濃度的甲醇燃料來測量電池的效能並與之前溫度的圖表做比較，選出最合適的直接甲醇燃料電池反應環境作為添加雜質實驗的標準環境。接下來我們在研究過程中發現，其實中間產物可能對電池有正面的效果，我們發現甲酸、甲醛等對直接甲醇燃料電池的電源輸出有正面的影響，例如電壓穩定性與電壓強度的增強。在研究中，我們將甲酸等加入燃料電池的燃料(甲醇)內，模擬甲醇因不當保存而產生的雜質，針對添加物的濃度做些調整，以電池的開路電壓(OCV)、平均輸出電壓、以及單位面積的電流密度作為評比電池效能的標準，並找出甲酸等對燃料電池效能的影響，並進一步找出最適合的電池燃料配置。

現今日常生活充斥著有機污染物，然而處理含有有機污染物的廢水需要極高的成本，有鑑於此，我們參考Fenton Reaction，從成本、毒性、活化能、操作方便性、二次污染及經濟效益的多方考量下，選擇以分子篩來固定鐵、鈷、鎳、錳、鋅之金屬離子，並決定以鐵分子篩為研究主軸，並探討其催化過氧化氫對有機物的分解。鑒於粉末狀的分子篩容易流失，我們以陶土固定分子篩，製作成反應杯槽，發現了分子篩與陶土的結合性。接著藉由二氧化碳感測器及光譜儀來感測有機物的分解速率，在控制溫度，濃度等條件下，探討分解有機物的反應及其反應時的特性。由實驗結果得知，分子篩能有效分解簡單醇類、氯仿、四氯化碳及indigo。使用0.35克陶土鐵分子篩，1M 以下的雙氧水50ml，其分解異丙醇所生成二氧化碳的速率可達0.34-0.55ppm/sec 之間（3.1-4.9×10-9mol/sec），此外有機氯化物分解後生成無毒性的氯離子；indigo染料分解後褪色。本實驗證實，陶土鐵分子篩：一、可以重複使用；二、可在較低濃度環境下運作；三、在酸性較弱環境下運作；四、可在低溫環境下運作(10℃)；五、不須對大量鐵離子做沉澱回收的工作（此五點優於Fenton Reaction）。相較於TAML 等人工合成的催化劑雖有避免污染的優點，但卻有無法重複使用的缺點，綜合以上幾點看來，陶土鐵分子篩在操作方便性及經濟與環境保護上具有相當的潛力及價值。With organic pollutants everywhere and the high cost to dispose of them, this study, a two-stage experiment, aimed first to evaluate the efficiency of zeolite with different metal ions and then to compare their rates in reacting to the decomposition of organic matter with hydrogen peroxide as the catalyst. Since zeolite powder can be easily washed away, we tested zeolite with clay to hold such metal ions as Fe, Co, Ni, Mn, and Zn and finally used the Argillaceous Fe-zeolite for its superiority on the basis of cost, toxin, activation energy, easy operation, and contamination. A carbon dioxide sensor and a spectrometer for visible light were used to measure the decomposition rate of organic matter under controlled temperature and resolution concentrations. The results of the experiments showed that zeolite achieved excellent effects in decomposing organic chlorides such as lower alcohols, chloroform, and carbon tetrachloride. When 0.35g of zeolite and less than 1M of hydrogen peroxide resolution were used the rate of carbon dioxide production reached 0.34-0.35 ppm/sec (3.1-4.9x10-9 mol/sec). The decomposition of organic chloride produced nontoxic Cl and the indigo dye faded after it was decomposed. Our experiments proved that Argillaceous Fe-zeolite has the following five advantages over Fenton Reaction. First, it can be reused. Second, it performed well at lower concentrations. Third, it worked well under weak acid conditions. Fourth, it worked at a lower temperature (20℃). And finally, there was no need to recycle a large amount of Fe ions. Argillaceous Fe-zeolite was also found to be superior to TAML, which has the advantage of avoiding contamination but is not reusable. The above observation and discussion demonstrate that Argillaceous Fe-zeolite possesses very significant value in terms of easy application, economy, and environmental protection.

動脈硬化是個致病率和致死率相當高的慢性發炎疾病，為台灣十大死因之一。在病理過程中血中濃度過高的膽固醇為動脈硬化的一大危險因子，其會誘發一連串的發炎反應驅使血管壁內皮細胞功能喪失，血壓上升，平滑肌細胞增生等。Statin 是臨床上十分有效的降血脂藥物，雖然其作用機制已知在於抑制膽固醇合成酵素 HMG-CoA reductase 而有降血脂功效，但近年來探討 statin 在抗發炎方面的作用也漸受重視。nitric oxide synthase (NOS) 代謝產物如 nitric oxide (NO)，cyclooxygenase (COX) 代謝產物如 prostaglandin (PGE?、PGI?)，及 heme oxygenase-1 (HO-1) 代謝產物如 carbon monoxide (CO)，均有文獻指出可以改善血流，而可能在動脈硬化上扮演保護角色。相反的，matrix metalloproteinase (MMP) 的表現會誘使更多的免疫細胞浸潤到血管壁，並增加動脈硬化斑破裂，引起栓塞和中風的發生。在此實驗中，我們利用培養的大鼠主動脈血管平滑肌細胞作為研究材料，發現了 statin (lovastatin 、pravastatin、atorvastatin 、fluvastatin) 具有一些和降血脂無關的直接保護血管壁能力。包括會增加 interleukin-1β (IL-1β) 所誘導 iNOS 蛋白的表現及NO 的產生; statin 本身會增加 COX-2 和 HO-1 蛋白的表現及 PGE? 和PGI? 的產生，及抑制 MMP-2 和 MMP-9 蛋白活性的表現。此外分析調控 iNOS 基因轉錄最為關鍵的基因轉錄因子 NF-κB，發現適量的 statin 會增加 IL-1β 活化NF-κB 的作用。值得一提的是雖然適量 NO 有維持血管恆定的功能，過量時則會造成血壓過低休克的現象，這就是細菌感染後因內毒素 lipopolysaccharide (LPS) 作用引發敗血性休克的主要原因之一。為更進一步釐清 statin 是否會影響受細菌感染病人的生命危險，我們也探討 statin 對LPS 作用的影響。結果發現 statin 反而會抑制 LPS 誘導大量 iNOS 蛋白的表現，NO 的產生及 NF-κB 的活化。這些新的實驗結果提供更多證據支持 statin 可以藉由維持血管舒張，減緩血管壁的發炎反應，穩定動脈硬化斑的作用，以有效控制動脈硬化各個病程的進展。這發現能讓我們更透徹明白 statin 的作用，且對將來研發 statin 在心血管疾病方面新的臨床治療用途是有所助益的。 ;The 3-hydroxy-3-methylglutaryl (HMG)-CoA reductase inhibitors, statins, are potent inhibitors of cholesterol synthesis and have wide therapeutic use in cardiovascular diseases. Recent evidence, however, suggests that the beneficial effects of statins may extend beyond their action on serum cholesterol levels. In this study, we investigated the effects of lovastatin, pravastatin, atorvastatin, and fluvastatin on cultured rat vascular smooth muscle cells. We found statins can inhibit LPS-induced iNOS expression and NO production, while they can potentiate IL-1β-elicited responses. Moreover, statins themselves can stimulate COX-2 expression, PGE?, PGI? formation, and HO-1 induction. In contrast, statins can inhibit basal and IL-1β-induced enzyme activities of MMP-9 and MMP-2. In studying the activity of NF-κB, which plays an important role for iNOS gene induction, we found that statin can increase IL-1β-induced NF-κB activity, while inhibit that induced by LPS. All these results suggest that stimulation of iNOS expression in the presence of IL-1β, togeth1er with the increased COX-2 and HO-1 expression might contribute to the beneficial effects of statins in atherosclerotic process in terms of vasodilation and inhibition of smooth muscle cell proliferation. The inhibition of MMP activity might enhance plaque stability and reduce the development of atherosclerosis. All these results strengthen the pleiotropic actions of statins in anti-inflammation and anti-atherosclerosis.

It is extremely costly to care for elderly people who have suffered a serious fall. Thus, doctors welcome a device or method to identify people prone to falling, to reduce elderly health costs and enable those identified as “fallers” to take precautions. Recently, a Sound Wave Assessment (SWA) device was developed to determine if tested human subjects were prone to falling. It is based on the concept that all humans exhibit postural sway while standing stationarily. The device employs two sonar transducers, which emit and receive ultrasonic pulse signals. The first transducer is positioned on a tripod, while the second is attached to the lower back of the human subject. Each transducer emits ultrasonic modulation pulses, which are detected by the other transducer. The distance between the two transducers is calculated from the time taken for the pulses to travel from the sender to the receiver. Hence, we can measure the small displacement fluctuations of the standing human subject, both approaching and receding from a static transducer, as a function of time.

本文我們研究下列問題： 1. 何時心律會成穩定的狀態呢？ 2. 是否能建立出心律穩定的數學模型？ 3. 什麼樣的函數會使得心律趨於穩定？我們以xmp+i 表示第p+1 個訊息傳到第i 個細胞之前，第i 個細胞的舒張時間，而且xk 和 xk−m, xk−m+1,..., xk−1的關係為 In this paper we study the problems as follows: When will the rhythm of heart beats approach to a steady state? Can we set up the mathematics model with steady rhythm of heart beats? What kind of function will make the rhythm of the heart beating tend to be stable? The result of our study is as follows:

One of the anthropogenic influences on the permafrost landscapes is deforestation and breach of the surface cover at the road constructions. On these areas a development of various cryogenic and postcryogenic processes and features (thermosubsidence, knobs (bilars, baydjarakhs), ravines, small lakes - djyodje) is being observed. Such features can be observed on the territory of Megino- Kangalassky Region, which is situated in the Central Yakutia, as well. During the period from 1998 to 2003 the author carried out monitoring studies of cryogenic features along three roads. Ten areas measuring twenty square metres were put in all. During 6 years of research about 1520 measurements of cryogenic formations parameters were made. Based on the results, it is concluded that elimination of the shadowing effect by trees and removal of the surface cover along the roads have caused thawing of shallow-lying ice wedges, as well as development of various cryogenic processes and features. In this paper, the author presents the basic technologies which are used in road construction in permafrost areas and, based on the research results, proposes a set of measures for rehabilitation of the roadside areas.

我們得知從高雄糖廠及堆肥中篩出的嗜高溫好氧菌Geobacillus thermoleovorans T4，是本種細菌首先被報導具有纖維素分解能力的菌株，但目前僅止於學術研究階段，尚未實際應用於廢棄纖維素的分解上。本研究以廢棄農作物纖維素取代學術研究所用的羧甲基纖維素(Carboxymethyl Cellulose，CMC) ，將T4菌株置於稻稈與米糠培養基內，進行分解效能比較，再利用酵母菌的發酵作用進一步將葡萄糖轉化為酒精。本研究發現，在60℃的環境中，活化的T4 菌株可在CMC、稻稈及米糠培養基中生長繁殖且發揮其分解纖維素為葡萄糖的能力，尤其在米糠培養基中的分解效果最快也最好。此外，將生產的葡萄糖加入酵母菌之後，初步發現也能成功地進行發酵作用產生酒精，生質酒精的產出指日可待！;The Research on Using Geobacillus thermoleovorans T4 to Turn the Deserted Cellulose in Agriculture into glucose in Order to Produce Bio-Renewable Energy It has been found that Geobacillus thermoleovorans T4, a thermophilic aerobic bacterial strain isolated from a sugar refinery wastewater （55-60℃）in Kaohsing, Taiwan, can secrete thermostable endocellulase and hydrolyze carboxymethylcellulose （CMC）in some academic research, but it is stillunknown whether T4 hydrolyzes deserted cellulose in Agriculture. The aim of this study is to investigate the best conditions of T4 cellulase activity after mixing with deserted cellulose (such as rice bran and rice straw) by measuring the glucose concentration and bacteria number, and to produce the ethanol by activated yeast. T4 was added rice bran and rice straw medium, and cultured in 60℃ for 10 hours. The number of T4 and the concentration of glucose were measured every two hours. The best conditions were examined by comparing the hydrolyzation efficiency of T4 in different cellulose medium. We observed that T4 grew efficiently in different cellulose medium and hydrolyzed cellulose into glucose, especially in rice bran medium. The yeast also converted glucose into ethanol. Our research may shed light to the development of bio-renewable energy!