中文摘要 重金屬對人體傷害的無遠弗屆-人人皆知，根據流行病學的研究指出，長期處在 重金屬含量過高之地區，易造成生理病變。比方說，長期生活在砷含量高的環境中， 易導致肝臟、周邊血管及神經系的損害，各種癌症的發生機率也大為提高。 本實驗以中國倉鼠卵巢細胞（CHO-K1）為材料，利用微核偵測技術(Michronuclei, MN)及姐妹染色分體互換(Sister chromatid exchanges, SCE)之誘引來探討亞硒酸鈉 (Sodium selenite, NaSeO2) 及亞砷酸鈉(Sodium arsenite, NaAsO2) 的交互作用 (interaction)。實驗結果顯示亞硒酸鈉本身不會造成MN 的增加，但讓SCE 頻率增加； 亞砷酸鈉會誘引增加MN 和SCE。亞硒酸鈉前處理不會減少亞砷酸鈉誘引之SCE 和 MN，但可微微降低亞砷酸鈉抑制雙核細胞(Bunucleated cells, BN)的形成，但是不明 顯。本實驗結果未能看出亞硒酸鈉前處理與亞砷酸鈉有明顯的交互作用，但發現亞 硒酸鈉會增加SCE 頻率，因此亞硒酸鈉做為保健食品值得進一步的關切及探討。 英文摘要 It is well-documented that exposure to heavy metals could cause seriously adverse effects to humans. Epidemiological evidence has shown that illness is frequently observed in residents living long-term in heavy metal contaminated area. For example, arsenic exposure was associated with increased incidences of liver diseases, peripheral vascular and neurological diseases, as well as cancers. In this report we investigated the interaction of selenite and arsenite on micronuclei (MN) and sister chromatid exchange (SCE) induction in Chinese hamster ovary-K1 (CHO-K1) cells. The present results demonstrated that sodium selenite by itself did not induce MN, but it did cause the increase of SCE frequency. Sodium arsenite significantly induced MN and SCE in CHO-K1 cells. Pretreatment of CHO-K1 cells with selenite could not reduce MN and SCE frequency induced by arsenite, but slightly prevent the cells from arsenite-induced inhibition of binucleated cell formation. In the present study, no significant interaction between sodium selenite pretreatment and sodium arsenite was observed. However, sodium selenite was unexpectedly found to enhance SCE frequency in CHO-K1 cells. The application of selenite as health protection agent warrants our concerns and further investigation.

本研究以模擬實驗探討波浪在斜坡海灘上的行為。實驗在長1.8公尺、寬0.75公尺的透明水波槽中進行，以長0.90公尺、寬0.60公尺的木板在深水區產生單峰波向淺水區前進，同時以數位錄影機錄影後進行分析。結果發現單峰波由深水進入淺水，波速會變慢，但當波高對水深的比值增加到一定值時，波速隨水深變淺而變快，波高也變高。當比值繼續增加，波前方的水面形成垂直的水牆，接著波就碎了。如果坡度較緩，碎波點會離水岸線較遠，水牆維持的時間也較長。有趣的是，水波槽中的單峰波移動時，有蠕動現象，波寬會伸縮，波高會起伏，波速也會些微地忽快忽慢。 ;This study simulates the behavior of the wave on a sloping beach. Experiments are performed in a sloping wave tank. A paddle wave maker at the deeper end generates single crest waves. To analyze the wave height, speed and breaking point, a digital camera is used. The results show that when the wave moves toward the coast, the shallower the water is, the slower the wave moves. But when the ratio (wave-height/water-depth) exceeds a critical value, it turns out that when the water is shallower, the wave speed becomes faster and the wave height, higher. As the ratio keeps on increasing, the front part of wave becomes a vertical water-wall, and then breaks. If the slope is gentler, the breaking point will be farther from the coast and the water-wall will keep for a longer time. An interesting phenomenon is also found that a single crest wave squirms with slightly undulated changing of width, height, and speed while it propagates in the sloping wave tank.

在「植物的秘密生命」這本書中提到植物在電場或磁場中會生長的更好，我最近正好學到了電磁場如何產生，以及一些產生電磁場的方法，所以本研究即利用螺形管線圈來產生均勻的電磁場，並用運用安培右手定則來控制磁場產生的方向，來探討電磁場在不同條件下對實驗植物－綠豆生長的影響。本實驗使用銅質線圈纏繞鐵碗的方式，且通入不同大小電流來改變磁場的強度，以探討對實驗植物－綠豆生長的影響。本研究所得到的結果顯示：(1)綠豆在通入電流50mA 時所產生的電磁場可促使綠豆達到最好的生長效果；(2)栽培綠豆環境中所架設的電磁場較高，可使綠豆表現出較為明顯的生長速率；(3)促進綠豆的生長並不需要二十四小時都通入電流；(4)綠豆在N 極朝上的電磁場中生長的速率較S 極朝上的磁場中要快；(5)在較低溫度環境中，電磁場促進綠豆生長的效應較為顯著。;It has been mentioned that the plants will grow faster in the electronic or magnetic field in the book of “the secret life of plants”, I learned about the knowledge and methods of how to produce the electromagnetic field just now, I use the screw wire to produce electromagnetic field and control the direction by the Amplifier’s rule to investigate the growing speed of green beans under electromagnetic field in those different temperature. In this experiment, I use the wire to screwed around the ironed bowl, and make different electric current to generate different strength of electromagnetic field, the results indicate that (1) The faster growing speed will only exist in some strength of electromagnetic field, (2) The higher ironed bowls that full of screwed wire will result in the faster growing speed, (3) It will not need electromagnetic field all days, (4) The green beans will grow fast in those north magnetic pole than those in south magnetic pole, (5)The effect of electromagnetic field to increase growing speed will be significant in higher room temperature.

為了瞭解黃斑黑蟋蟀叫聲和溫度之間的關係，我應用物理熱平衡原理，自製水浴槽控制環境溫度，以電腦錄音程式分別錄製15℃、20℃、25℃、30℃、35℃時的蟋蟀叫聲，利用Sound Forge軟體分析並比較不同溫度下的各種特性。結果發現所分析的chirp、chirp period、syllable period、pause均和溫度呈負相關，而單位時間內的叫聲次數則與溫度成正相關，並推導出15~35℃範圍內蟋蟀叫聲次數與溫度成正相關的公式。最佳直線為：﹙15秒內叫聲次數＋19.385﹚÷2.483=當時溫度﹙℃﹚，呈高度正相關﹙相關係數：0.9398﹚。本實驗也比較母蟋蟀對不同溫度下雄蟋蟀叫聲的偏好程度，發現母蟋蟀較偏愛25℃時的雄蟋蟀叫聲，此結果可能與雄蟋蟀在25℃時叫聲的波形最為穩定有關。為探測蟋蟀觸角上的溫度感受器位置，我們曾將不同部位的觸角加以剪除，結果顯示觸角剪除面積越大的雄蟋蟀越不傾向鳴叫，但無法證實是否為溫度偵測異常所導致。本實驗除供學術研究外，因黃斑黑蟋蟀在世界各地均有分布，所以希望能藉由分析溫度與蟋蟀叫聲的相關性實驗，未來嘗試能探討其在不同環境的適應行為亦或種化的可能性。另外，全球暖化問題日益嚴重，也希望能藉由相關實驗探討暖化對蟋蟀生態上的影響。最後，我們的實驗證明了蟋蟀叫聲和環境溫度確實有極大的關聯性，未來或許能參考蟋蟀感覺溫度的機制，製作出一個天然零污染的溫度計。 To find out the relationship of cricket’s calling and temperature, we use a hand-made water-bath tank to control the temperature and record the callings with microphone and software, Sound Forge. After analyzing the collected data, we’ve found that cricket’s chirp, chirp period, syllable period, and pause are indeed affected by temperature (15 degrees - 35 degrees C). Moreover, we also compare female cricket’s preference to the callings, and the result indicates that female cricket’s preference is changing with temperature. In the last, we tried to find out where the thermoreceptors are by cutting out the antennae. After cutting, crickets tend to not to make any calls at all, so we conclude the antennae might play an important role in sensing and calling. The experiment proves that this communication system is temperature coupled. Because the cricket, Gryllus bimaculatus, is a worldwide species, we may learn the accommodation or the possibility of performing a new species by researching the relationship of temperature and cricket’s callings. By the way, the Green House Effect is getting more and more serious, so we want to search for the influences on crickets that are caused by Green House Effect. The last but not the least, according to the report, maybe we can investigate the mechanism of sensing temperature and then make a natural thermometer that is no pollution in the future.

The Bay Mau Lake inside the Thong Nhat Park in Hanoi is the second largest lake in\r Hanoi. The lake serves not just as an equitable tank for the rainwater, as a reservoir for\r intake of wastewater, as a wastewater treatment pond, but also as a recreation area for\r Hanoi people and visitors from other provinces. The Bay Mau Lake is polluted at\r alarming level.\r The author has carried out a study on the water pollution level and the sources of\r pollution. The study revealed that the lake polluted mainly by domestic wastewater\r discharging from living quarters with total about 800,000 inhabitants. The author\r demonstrated different measures to reduce the pollution level in the lake such as raising\r environmental awareness for the public, a simple, reasonable cheap measure, which can\r be both realistic and effective. Besides, the author proposed to use bio-treatment of the\r water discharging into the Bay Mau Lake, building of a pumping station, fish farming...\r The clay-paper fibre mixture is used for the biological treatment of wastewater at the\r inlets. The advantage of this method is that it is a simple, cheap and realistic solution.\r Treatment of the water using clay and paper fibre mixture for filtration and plantation of\r aquatic plants as lotus and water lily together will provide oxygen for the aquatic\r environment of the lake. As results of the study, it is recommended to use both methods,\r namely raising environmental awareness for the public and biotreatement method using\r clay and paper fibre, to improve water quality of the lake.

在正n邊形的n個頂點各放一面鏡子，並將其中一個鏡面順時針轉動90°/n，將雷射光線沿該點切線方向射向此鏡面，使光線在正n邊形各頂點的鏡面間反射，本文主要在探討光線在各鏡面間反射路徑之規律性與其應用，研究分為兩大部分：一、將雷射光線射向一順時針調整角度θ=90°/n的鏡面且其餘鏡面皆不轉動之情形下，探討雷射光線的反射路徑之規律性及尋找正多邊形邊數與反射數間的關聯性。二、在正n邊形的n個鏡面中，每個鏡面可依順時針或逆時針旋轉一個角度θ=90°/n或是不轉動。此部分我們只針對n為偶數的情況去探討，我們採用數學上函數的對應方式取代了物理上反射原理的路徑操作，由數學的模式去探討鏡面調整的規則，擬訂出適當的策略調整鏡面的轉動，並找出最大反射數。

Many biological processes in the human body take place in intracellular environments under crowded conditions. This means that such cellular activities occur in the presence of inert macromolecules. These macromolecules are thought to have large effects on reaction rates and equilibria. However, under conventional conditions, these same processes are studied in vitro under uncrowded conditions in dilute buffers and therefore not reflective of physiological conditions. Hence, this project aims to study the effect of macromolecular crowding on antigen-antibody reactions in an attempt to mimic intracellular environments. It also aims to highlight the importance of introducing crowders as a factor of study in conventional in vitro experiments involving cellular reactions. The model reaction investigated involves human collagen type 1 on skin samples and specific antibodies against these antigens. Inert macromolecules of defined hydrodynamic radius and charge were added into the reaction media while quantitative tests are conducted to determine the effect of crowding on the rate and extent of reaction. The macromolecular crowders used were Dextran Sulphate, Ficol170, Ficol1400 and Polyvinylpyrrolidone360. Results were than analysed with the Metamorph 7 imaging system to determine signal strength under crowded and non-crowded conditions. At high primary antibody dilutions, skin samples incubated with a primary antibody solution crowded with a combination of inert macromolecules showed improve signal strength. This could be because crowding tends to shift reaction equilibria such as to minimise the amount of excluded volume, leading to association of the antigen and its specific antibody. This is known as the excluded volume effect. In addition, crowding the primary antibody solution helped increase the rate of reaction. The results also suggest that the addition of crowders may reduce the amount of antibodies needed per sample and decrease the necessary incubation time. Therefore, antigen-antibody reactions can be done more cost-effectively, However, the presence of charged macromolecules such as Dextran Sulphate has an inhibitory effect. Further work is also needed to establish whether macromolecular crowding enhances specificity and thereby reduce autoflourescence of the skin sample.

The Latin American and Caribbean regions have one of the biggest cultivable areas in the world, calculated at around 576 million hectares. Nevertheless, 16% of this land its affected by a kind of soil degradation. Previous studies have shown that the vesicular- arbuscular mycorrhizae (VAM) can fix phosphorus and other elements to plants, is an important micronutrients transporter, increases the water potential of plants, can bioremediate the lands affected and prevents lixivation. These effects are very convenient and can replace the chemical fertilizers which produce collateral damage to the environment. For the reasons mentioned above, this prototype for the production of vesiculararbuscular mycorrhizae, denominated as a biofertilizer, is presented. The prototype consists of an aeroponic system which disperses, in aerial form, nutrients to the host/trap plant roots where the fungus produces its mycelium web. The fungus propagation consists of an artificial union of isolated and identified spores of the mycobiont ,which we want to propagate, with the trap plant roots. The specimen identification consisted in a staining and clarification method (Phillips- Hayman), and a taxonomical identification. In order to prove the (VAM) benefits, two experiments using Sorghum spp. were carried out. One consisted of a comparison between the plants with VAM and a control without VAM. The second one consisted of a chemical comparison between control/fertilizer/ VAM plants. These two experiments were subjected to a water stress test for 10 days. The prototype achieved a production of roots mycorrhizae between 50-65% of colonization. The taxonomic identification corroborated that the mycobionts propagated and the controls were the same species. Experiment One demonstrated that the mycorrhizae treatment has more height, stem diameter, fresh/dry weight than the control which doesn't have VAM. We also conducted the Student’s t Test to check the previously mentioned hypothesis. In Experiment Two, the control and fertilizer treatments had a similar percentage of Nitrogen and Potassium, and the mycorrhizae treatment significantly increased these two elements; nevertheless, the fertilizer and mycorrhizae treatment obtained a similar percentage of Phosphorous. The water stress test was for 10 days - one month after planting. The results were: the fertilizer and mycorrhizae treatment had the same resistance to the stress, the mycorrhizae recovered faster from the stress and the control specimens presented a lower shrivel percentage than the other plants. One of the principal gains which this prototype has is that the trap plant doesn't die after collection, and the plant only needs to be inoculated once in the plant’s life because we only prune the roots. In nature 90% of plant species present some type of mycorrhizae association, hence the feasibility of this prototype for introduction, use and application of the fungus as a biofertilizer. 1 Centro de Bachillerato Tecnologico Industrial y de servicios N°24 2 Instituto Tecnologico de Ciudad Victoria

如果n-1 個正四面體在n 個面的多面體棋盤中翻轉(如：正八面體、正二十面體、五邊雙菱錐及三角錐台棋盤)，則正四面體相互之間會受到很多限制。本研究為探討n-1 個正四面體在n 個面的多面體棋盤中，利用其翻轉的特性，翻轉至相鄰空格，進而完成：1. 每個正四面體的底面及側面均能「同色共面」。2. 在滿足以上的兩個條件下做「數字排序」。3. 探討每個遊戲在各階段是否有解的情形。最後，將遊戲推廣至正八面體在多面體棋盤中的翻轉。If n-1 pyramids can turn in the chessboard of n planes pyramids (ex: 8 planes pyramid, 20 planes pyramid, 5 sides bi rhombus pyramid and triangular pyramid chessboard) there are lost of restrictions between these pyramids. This research is discussing about the n-1 pyramids in the chessboard of n planes multi pyramid, which have the peculiarity of turn that can turn to nearby space. We found that: 1. Each pyramid’s button plane and aide plane can be “same plane same color”. 2. Matching about 2 conditions then can do “order by numbers”. 3. Search for answers of each levels in each game. Finally, the game will be propagated to eight planes pyramid can turn on the chessboard of multi pyramid.

Based on environmental protection and new energy development, the energy containing low pollution and having high efficiency becomes more popular. The fuel cell is an emerging technology, which is the reason why it is considered as Green energy. However, high-price membrane electrode assembly (MEA) inhabits the development of fuel cell. Among these components in MEA, platinum-based electrode leads the most cost. We select the multi-walled carbon nanotubes (MWNTs) as catalyst support to improve electrode material performance and reduce Pt utilization. But the particle size and dispersion of platinum as well as character of catalyst support may significantly affect the efficiency of electrode.燃料電池因為具備低排放污染、低噪音及高轉換效率等環保特性，故被稱為綠色能源，可取代內燃機作為發電之能源系統。然而燃料電池中觸媒粒徑大小、分散情形以及使用不同的觸媒載體，均有可能會影響其電觸媒的催化效能，所以我們以「觸媒粒徑大小」、「觸媒分散情形」為主軸，著手以下的研究。本實驗在不同的酸鹼值下製備燃料電池的電極材料，並以電子顯微鏡等器材，觀察已披覆在電極上白金觸媒的特性，探討不同酸鹼環境對白金觸媒粒徑大小的影響，並探究白金觸媒粒徑大小與其發電效率的關係。

台灣通史「婆娑之洋，美麗之島」，身為台灣人，應知台灣事，本研究本維 基百科（Wikipedia）所揭示的「海納百川，有容乃大」精神，以Apache 網頁伺 服器為平台，MySQL 為資料庫，利用AJAX、PHP 網頁技術，並使用Google Maps 提供之服務，建立符合Web 2.0 精神的的b04地理資訊共享系統。在此系統下， 我們允許教師(編輯者)以合作方式上網提供與課程相關的地理資訊，並可進行資 訊修改或刪除，希望集合所有教師之力，提供給學生最豐富、最正確的b04地理 資訊系統。 In this paper, we propose a new map-based collaboration environment “MyGIS” for geographical information distribution. In MyGIS, the publishers/teachers can easily collaborate to annotate a spot with contents such as messages and links. The annotations of a spot are updated on the map in real-time which enables the receivers/students to correctly study at anywhere and anytime. To ensure the trustfulness of the content, the publishers are authenticated using login mechanism. In this study, several technologies inc lu ding Apache, PHP, MySQL, Ajax are involved. Finally, We implemented MyGIS on Google Maps and evaluated its feasibility and usefulness.

車輛遭竊以及酒醉駕車對社會安全造成的危害普遍存在於世界各先進國家。以目前看來，此問題並非嚴刑峻罰就能解決的。若本研究計畫將來普及應用於大眾，必定可以同時減少車輛遭竊之機會和預先防範酒醉者開車駕駛。 生物特徵泛指指紋、虹膜、臉型、聲紋甚至DNA 等等，它的特性即每個人均具有此獨一無二的性徵，加以應用就如同無法被仿造或偷竊的身分證明。若車主能利用自己的生物特徵為車輛上鎖，即可防止車輛遭竊。 視覺密碼學是屬於資訊隱藏技術的一種，藉由兩張以上的影像疊合後呈現其中隱藏之機密資料。雖然視覺密碼學原先純粹是為了加密資料，但我們將之加以修改，設計出複雜且無意義的偽裝影像，使其不易被酒醉後神智不清的人疊合成功而讀出其中的加密資料。 結合生物特徵及視覺密碼學，即可設計出一套可安裝於車上的系統，不僅防止車輛失竊又能防止酒醉駕車事故的發生。 ;The damage which is caused to social security by car stealing and drunk driving universally exist in others developed countries. As things are, these problems can not be solved by severe law. If our project can be applied in the masses extensively, it must be able to prevent both car stealing and drunk driving. Roughly speaking, biological characteristics mean fingerprint, iris, face, pronunciation and DNA. Their uniqueness is a special feature that everyone has. If we can apply it more, the characteristics are identification that can not be imitated or stolen. If the car owner can use his or her biological characteristics to lock the car, the car stealing could be prevented. Visual cryptography belongs to a kind of information hiding. By stacking two or more images, user can recognize the secret information hiding in the stacking. Although visual cryptography is merely created for encryption at first, we modify it and design a complex as well as meaningless stego-image. That makes the unconscious driver who is drunk fail to both stack the images and recognize the secret information hiding inside. We combine biological characteristics with visual cryptography and design a suit of system that is able to install in the car. This system can prevent not only car stealing but also drunk driving.