水生食蟲植物絲葉狸藻 (Utricularia gibba) 是非常獨特的，它不但跟其他植物一樣能行光合作用，且具備捕蟲囊捕捉水中小生物，補充生長所必需的營養元素。捕蟲囊的構造精密卻不複雜，消化吸收主要靠囊內壁上的四爪腺毛，目前尚未有文獻實際以追蹤物質描述出整個共質體輸送路徑。我們是最先以螢光染劑 (carboxyfluorescein) 及共軛焦雷射掃描顯微鏡(confocal laser scanning microscope) 成功地描繪出捕蟲囊共質體運輸路徑。同時我們也以對細胞無害的食用色素，進行相同的實驗觀察。發現食用色素不但成本低，且較螢光染劑有更多的優點，如觀察時間較不受限制等，非常適合用來研究捕蟲囊吸收物質的路徑，因此，本實驗的模式可以應用在其他水生植物運輸路徑的研究。;The aquatic carnivorous plant Utricularia gibba is very unique. It has not only the ability to undertake photosynthesis just like other plants, but also can trap and obtain the nutrients from the freshwater zooplankton. Its trapping organ is very sophisticate but not complicate. The digestion and absorption process inside the trap are mainly accomplished by the quadrifids structure. According to our knowledge, we are the first to introduce the phloem-mobile, fluorescent probe carboxyfluorescein (CF) and confocal laser scanning microscope (CLSM) to the study of the symplastic transport in the Utricularia trap. In addition, we use edible food colorings as tracers for this transport study. Both approaches turn out to be very successful in delineating the symplastic transport of the trap. But CF quenches rapidly so the observation time is restricted. On the contrary, food colorings don’t have these disadvantages; it is inexpensive, easy to perform, and the transport process is not fast. As a result, the study is easily to be completed. These methods will be very helpful in the studies of symplastic transport in other plants.

“熱巧克力效應”是當你將巧克力粉加入裝有熱水之馬克杯後用湯匙輕敲杯壁，可以聽出攪拌前後敲擊所得聲音頻率明顯不同，攪拌後巧克力粉溶解頻率會較高。一般論文的解答是「當粉末溶解的時候，藏在粉末裡的空氣就會跑出來，在空氣與水混合的環境裡，音速比在水裡低。當水裡不斷有空氣與水混進去時，這個容器的共振頻率和它裡面的音速有關，所以也會降低。因此你會聽到較低之音調，直到空氣全跑光。」但根據我的實驗我覺得這樣的解釋並不對。我們提出新的模型來解釋“熱巧克力效應”。When we put an ounce of dry chocolate powder into a mug filled with hot water, then tapping the side with your spoon will generate a sound. The pitch of this sound will rise after stirring. This is called “the hot chocolate effect”. According to a published paper, the explanation is “The air trapped in the powder is released as the powder dissolves. Since the speed of sound is lower in air than that in water, the speed of sound in the air-water mixture is lower than that in pure water. During that period while the air escapes the container, the resonant frequencies of the water, which depend directly on the speed of sound, will also be lower. Hence, you hear a lower tone until the air escapes”. However, our experiments clearly demonstrated that the explanation is plainly wrong. A new theoretical model is also proposed for the observed phenomenon.

大氣圈、水圈與土壤圈是構成自然環境的三大領域，三者之間相互的影響，原本這些空間都具有極大的包容力，亦所謂「自淨能力」，許多的物質進入其中皆會被氧化分解或稀釋而消失於無形。近年來由於工業發展、人口增加，產生大量的廢棄物，長期、密集且迅速的堆積於環境中，使得天然的自淨能力無法應付而失去功能，造成嚴重的後果。就土壤而言，雖有較佳的自淨能力，但是一受污染，除了嚴重破壞土壤品質之外，同時也會直接或間接污染水源 (如:地下水)及空氣，對動植物造成危害，並且難以回復，實不容忽視。本實驗探討:一、土壤淨化能力是否受到不同地區、不同土壤性質的影響。二、同樣的土壤，對不同的污染物(色素、肥料、重金屬)淨化能力強弱的影響。實驗結果顯示大肚山土壤過濾溶液中的色素、磷、及重金屬中的鉻、鎳、銅的能力較中寮及大甲土壤強，只有氮與鉀的過濾能力較大甲土壤差，所以這次實驗中大肚山土壤有最好的污染淨化能力。實驗結果將來也許可以應用於土壤處理場址之適宜性評估。The air, water and soil are three parts of the nature. They affect each other. In fact, they have the "self-purity ability" - they can disassemble many polluted thing by this kind of self-purity ability. These years, because the industry development and the population explosion make lots of waste, the self-purity ability cannot do its best. The soil has better self-purity ability, but if it is polluted, it will not only pollute the water (ex: underground water) and the air, but also damage the animals and plants. We cannot ignore the serious result. This experiment will discuss:1. If the soil self-purity ability is affected by different area and different soil specificity? 2. The different purified results according to the different pollutant (ex: color, fertilizer and heavy metal) in the same soil. Results showed that Da-Du-San soil had strong ability then Chung-Liao and Da-Cha soil in filtrated color, phosphor, chromium, nickel and copper in the solution, but had weak ability then Da-Cha soil in filtrated nitrogen and potassium. Therefore, Da-Du-San soil had the beast pollution-purity ability in this experiment. In the future, results may be applied to the suitability evoluation of the soil treatment place.

當我們在開車享受冷氣同時，此時冷氣水正一滴一滴的滴水，造成水資源的浪費，在環保意識抬頭的今天，我們即針對此一問題進行研究，主要將冷氣水回收起來，並運用在補充雨刷水箱或者提供引擎水箱或冷凝器降溫作用，是否達到提高引擎工作性能及降低冷氣冷房效果，進而達到「資源回收」的。實驗結果證明在補充雨刷水系統最符合環保概念；另在引擎水箱噴水作用時，可縮短風扇運時間並增加停止運轉時間，可增長風扇使用壽命，對下水管溫度亦可降低，可防引擎過熱；在冷凝器噴水作用中亦能明顯提升汽車冷房效果。When we enjoyed driving with cool air from air-conditioning, the condensed water from air-conditioning system is dripping from the system drop by drop. It caused the issues of the waste of water resource. Facing the greater public awareness of environmental protection issues in Taiwan, we are focusing on this issue to have further research. The idea is to re-cycle the air-conditioning condensed water and re-fill it in the water tank of wipers, the water cooling tank of engine or the cooling system of condenser. The purpose is to improve the performance of engine and enhance the cooling efficiency of air-conditioning system. It is helpful to meet the objective of water resource recycling. The result of experiment has shown that re-filling water in the water tank of wipers meet the goal of environmental protection well. Also, the water injection in the water cooling tank of engine could reduce each operation time of cooling fan and increase the idle time of cooling fan as well. It prolong the equipment life of cooling fan and lower the operation temperature of Low water pipe which prevent the engine overheating. Meanwhile, It is proved that the water injection in the cooling system of condenser can enhance the cooling efficiency of air-conditioning system.

Purpose of Research\r Colour blind individuals make up a large part of the population but are not usually considered. I aim to design an interactive guide that can be used when designing websites, social networking pages and various digital presentations to ensure that they are legible to colour blind individuals, by determining which colours generally confuse both red-green and blue-yellow colour blind people.\r Procedures\r I e-mailed my test, which consisted of 240 slides depicting every possible combination of the 16 basic web colours in the form of a frame, a heading-appropriate sized text and a paragraph-appropriate sized text, to a group of red-green and blue-yellow colour blind individuals, as well as an equally sized control group. I asked them to flip through the slides at a comfortable pace and note the slides that were difficult to read offhand, thus getting an idea of which colour combinations are not suitable for colour blind individuals, as well as the combinations that are not visible to those with normal colour vision.\r Data\r I wrote up my results using tables and stacked column graphs to determine which colour combinations were not visible to an unusually great number of test subjects. I then represented the information I collected in the form of a flash guide. This interactive guide allows one to choose a background colour from a wheel of 16 colours, and then be given a choice of appropriate text colours. The user is also presented with an example of the combination that was chosen, showing how the text appears on the background. The guide is easy to navigate and understand, can be posted on the internet or e-mailed, and is not overly technical so it can be used by designers as well as ordinary internet users.\r Conclusion\r Colour blind people are easily confused by colour combinations which might seem clear to us. My hypothesis was proved as I was able to set up a guide that would make it possible for both colour blind individuals and those with normal colour vision to read the text on websites, social networking pages and digital presentations.

因國際原油價格上漲，因此對研究及發展具潛力的生質柴油引起高度興趣。傳統以加熱方式在適當觸媒下將低碳數醇類與油脂轉酯化生成生質柴油。但以強鹼為觸媒使用過量會讓部分反應轉為皂化。而利用微波，使微波腔在極短的時間內產生電場，使具有電偶極的分子朝向電場方向迅速排列而產生轉動，快速地增加分子間的碰撞頻率而加速反應，但不破壞分子結構。也嘗試利用超音波來生產生質柴油。試驗方法為利用SD-22 柴油引擎測試所生產的生質柴油，並收集廢氣檢驗，評估其污染性。實驗後發現，微波能將一般加熱所需的60 分鐘大幅縮短至4 分鐘，甲醇溶液能生產較多的生質柴油。其中1.40%氫氧化鉀甲醇溶液且油與醇莫耳比1：9 效果較佳。氫氧化鉀甲醇溶液0.80%且油與醇莫耳比1：5 效果亦佳。製成的生質柴油能讓引擎發動，廢氣處理後經分光光度計檢驗氮氧化物，生質柴油確實能有效減少氮氧化物污染。 Due to the fact that the price of international crude oil raises , people look for alternative energy resources actively. Therefore, I have great interests in doing researching and developing the biodiesel through many ways. The traditional way is to transesterify the low carbon alcohols and fats with the suitable catalysts by means of generally heating. However, the overuse of strong bases as catalysts may turn the experiment result into saponification reaction. This study made good use of the microwave to generate electric fields in the microwave cavity in an extremely short time. Owing to the effect electric fields exerted, the molecules with electric dipole momentum rearranged to rotate rapidly .Consequently, the frequency of collision among the molecules increased without destroying the molecule structures. Also, I applied SD-22 diesel engine to test the produced biodiesel and examined the collected exhaust to evaluate its pollutant. According to the results of these experiments, the conclusion was as following: the microwave could shorten the Transesterification reaction time from 60 minutes to 4 minutes. The methanol solution could produce more biodiesel than the ethanol solution. Besides, the 1.40 wt ％ Potassium hydroxide solution with the molar ratio of oil and methanol 1:9 had the best efficiency in producing biodiesel. On the other hand , the 0.80 wt ％ Sodium hydroxide solution with the molar ratio of oil and methanol 1:5 also had the great efficiency. All the biodiesel we produced enabled the diesel engine to run smoothly. The exhaust inspected from the examination of Spectrophotometer resulted that the biodiesel did less NOx pollution to the environment indeed.

1. 碘酒的配製 碘是非金屬元素，符號為I、而碘分子為I2，具有消毒殺菌作用。碘(I2)不溶於水，在酒精中的溶解速率慢，但非常容易溶於含有碘離子(I－)的水溶液，因I2容易與I－構成錯離子 ，易溶於水。因此要配製碘酒時，要先將1.0克的碘化鉀溶於10毫升的蒸餾水後，加入碘2.5克與酒精50毫升，攪拌使碘完全融解，再加蒸餾水配成100毫升，即成為常用的皮膚消毒劑。 2.碘酒不適於消毒傷口 碘酒與雙氧水一樣，是醫師用來消毒無傷口的皮膚以便進行手術的，因為傷口經不起任何刺激，過於刺激會抑制傷口癒合，有時還會造成疤痕。傷口的消毒最好使用無菌的生理食鹽水沖洗。 3.碘酒與紅藥水不能混用 因為碘酒含有碘(I2)，而紅藥水含有汞(Hg)，兩者相遇會產生碘化汞(HgI2)，碘化汞為劇毒物質，對皮膚黏膜有強烈的刺激作用，可能會引起黏膜潰瘍，所以碘酒和紅藥水不能混用或先後使用。 4.碘酒可用於檢驗澱粉 碘與澱粉的反應非常靈敏，一般稱為碘－澱粉反應。衛生單位曾利用碘－澱粉反應以檢驗餐館碗盤的清潔。碘－澱粉反應的呈色可能是藍、藍紫、棕、甚至粉紅，太濃時可能成黑色。澱粉的組成大約可分為兩類，直鏈澱粉與支鏈澱粉。例如：可溶性澱粉含直鏈澱粉的成分較多，可溶於熱水形成膠體溶液，與碘酒作用則呈現藍色。澱粉遇碘酒會呈現什麼顏色，取決於澱粉中的直鏈澱粉與支鏈澱粉的比例。有的豆類所含的澱粉，幾乎是直鏈的，遇碘酒呈藍色，而糯米中的澱粉幾乎是支鏈的，遇碘酒則呈現紫色。 【動手做】 1.取急救箱的碘酒5滴，加水20滴後攪拌均勻，得黃褐色的碘酒稀溶液，作為檢驗澱粉之用。 2.取一粒生米，搗成粉末後，滴碘酒稀溶液於粉末上，觀察有何變化？ 3.另取一粒飯(熟米)後滴一滴碘酒稀溶液於飯粒上，比較與生米有何不同？ 4.以同樣的方式檢驗家裡的食物，例如：稀飯汁、餅乾、玉米等仔細觀察其顏色的異同。

Aims of the project Prevent youth from the -reckless use of the road .shoulder .Reduce the congested roads - Reduce accidents resulting - from the wrong use of the road .shoulder Idea of the Project The control system based road shoulder constantly and actors vis notice of the existence of any prejudice to the safety of road users to take the necessary action against them The beneficiaries of the System With health-Bouts With emergency situations The way the system works When any vehicle parked on the road shoulder remote sensors sensing the vehicle body and the issuance of the initial reference point against the vehicle on the shoulder of a road..And after a period of time known by the concerned and at the same time the system to identify the site and send information to the authority concerned in turn, take the necessary

安德遜蠅虎的卵期和幼蛛期共約需 22~26 天，需經歷 6 齡（6 次脫皮）而成年，每齡需要時間為23~32 天，從卵期到成蛛約3~4 個月左右，成蛛約可再活 3~5 個月，為不完全變態。安德遜蠅虎喜歡在食物充足和陰暗的草叢和牆壁底層活動，能適應的溫度範圍在 15~33℃之間。安德遜蠅虎的行為項目目前已知 14 種，其中 4 種與捕食行為有關（例如：頭胸部升降、潛行、撲擊、迂迴），3 種與爭鬥行為有關（例如：Z形舞蹈1、前足斜舉、奔逃）；5 種與求偶行為有關（例如：觸肢擺動、前足斜舉、繞行、Z 形舞蹈 2、交尾） 。但牠不獵捕比自己體型過大的獵物，主要以小型蠅類或彈尾蟲為食，其捕食獵物主要靠視覺或感覺毛，中前眼最遠可看到超過12cm距離的東西，跳躍力最遠可跳出15cm 的距離。對於蠅虎的分佈區域就觀察提出【蠅虎集中發散推論】 ：一區域內的各種蠅虎，必有其集中地。各種蠅虎由其集中地向外發散、擴張形成發散地，並與其他種蠅虎勢力範圍重合，形成多種蠅虎共存的共存區。Hasarius adansoni’s egg development and postembryonic development require 22~26 days. It takes 6 stadiums to become adults. Every stadium needs 23~32 days . The adults can live to about 3~5 months . There are 3~4 months from egg development to adult. Hasarius adansoni like to move in grass and at the bottom of wall where there is plenty of food and no light.The scope of temperature is 15℃ to 33℃.There are 14 elements of behaviors known for Hasarius adansoni, including 4 predatory elements（such as Raise andlowered cephalothorax、Stalk、Leap and bit、Detour） , 3 agonistic behavior elements （such as Zigzag dance 1、First leg erect、Suddenly run and leap）, and 5 courtship behavior elements（such as Palp waving、First leg erect、Circular detour、Zigzag dance 2、Copulation）.But they don’t catch captures which are bigger than themselves；their main captures are little flies and Homidia sp. Relying on their vision and sensation hair，Hasarius adansoni’s Anterior Median eyes can identify for up to 12cm；long jump can for up to 12cm. The inference of jumping spider’s distribution could be that different species of jumping spiders in an area are sure to have their respective center habitats. They spread from the center habitats to disseminated areas. Then they overlap with those of their species and form a shared section of multiple species.

此研究之重點在於如何建構一套人工智慧方法，人工智慧含多種進行方式，例如以類神經網路訓練近似於人腦之結構，而專家系統係於不同的科學領域內以自己之所知判斷。我們先以 John Holland 的 Genetic Algorithm （暫譯基因演算法，以下暫稱GA）的理論來實作出一套人工智慧系統之方法。Genetic Programming 係以ＧＡ為基礎之實作方法，主要的內容不出基本的演化定義；在這次的試驗中，首要為先定出程式欲演化成何種類型，在此我們先定義為排序型的演算法，經過分析實際程式之結構後定出適合基因元件的資料結構，分化為兩個部份進行，一為產生器，亦為突變，交配器，一為評審程式，亦為執行器，兩者使用相同之基本元件，再以不斷的交配和突變以達到全域最佳化。我們將兩種部份完成後，加上現有的PVM 分散運算函式庫來增加演算的速度。 Abstract The research mainly discusses AI. AI, involves in several types, for example, neural network(NN),which adopts human-like training method; Expert systems determine and make decision by what it has known. We will use John Koza's Genetic Programming theory to implement an AI system.Genetic Programming theory is based on GA. In our experiments, we have to define what kind of applications we want : a sorting application, which is divided into two parts - a producer (along with crossover and mutation operations) and an executor (along with judging operations), is an easyimplemented algorithm. Our program, also with PVM, will approaches global optimal after evolutions.

The purpose of this study is to set up a quick, easy and economical way to evaluate the ability of different concentration of various antibiotics to penetrate biofilm and establish the antimicrobial susceptibility patterns of various antibiotics. The susceptibility of five antibiotics upon sessile cultures of Bacillus subtlis ATCC 6633, Escherichia coli XL, Pseudomonas aeruginosa ATCC 27853, Staphylococcus aureus ATCC 29213 and Sarcina lutea ATCC 9341 were measured ATCC 27853, and S. aureus ATCC 29213 proved to be very difficult to eradicate, with only Gentamicin proving to effective at achievable drug concentrations, but the S. lutea ATCC 9341 biofilm was the most susceptible to the Penicillin. The results demonstrated that for biofilms of the same organisms, several hundred to thousand times the concentration of a certain antibiotic were often required for the antibiotic to be effect, while other antibiotics were found to be effective at the MICs. The concentration of antibiotic to penetrate the biofilm is proportional to the thickness of biofilm. Indeed, our research have already indicated that the use of MIC values to indicate antibiotics effectiveness is misleading, because MIC values can not represent the actual effect of anticbiotics on microbiologicals that have developed biofilm. The antimicrobial susceptibility patterns of antibiotics to various bacterial biofilm are different. The susceptibility of the mixed biofilm depends on the physical and biological change of biofilm. Our biofilm device offers a new technology for the rational evaluation of antibiotics effective against microbial biofilms and for the screening of new effective antibiotic drugs.此研究之目的是要建立一套操作簡便、快速且費用低廉之生物膜厚度產生方法，藉此探討不同生物膜厚度對抗生素抗菌之影響，進而完成抗生素對生物模之抗菌圖譜。本實驗將測試五種抗生素Bacillus subtilis ATCC6633，Escherichia coli XL，Pseudomonas aeruginosa ATCC27853，Staphylococcus aureus ATCC29213和Sarcina lutea ATCC9341之抗菌圖譜。試驗結果顯示Gentamicin對前四株試菌有較佳之穿透力，對S.lutea ATCC 9341則是Penicillin。實驗結果證明最小抑制濃度值確實無法實際有效地表達對已形成生物膜菌體之抗菌效果，要完全去除生物膜之抗生素濃度是為最小抑制濃度之數百倍到數千倍，而且抗生素用藥濃度隨著生物膜厚度增加而成比例增加。不同抗生素對不同菌株生物膜有不同之抗菌圖譜，混合菌株生物膜是否會促進或抑制抗生素之穿透力，端視其生物膜結構有無改變。本實驗方法可以做為一種快速檢定抗生素對細菌生物膜敏感性之新技術，同時亦可有效地篩選新的抗生素藥物對生物膜的抗菌效應。

原產於西非的「神秘果」，嘗了之後，30~200 分內，所有酸苦的東西嘗起來都是甜的。在深入蒐集相關資料後，我們發現神秘果有多種特殊效果，僅擷取以下幾種感興趣之方向來研究。〈1〉使酸苦的感覺變甜〈2〉解酒〈3〉消除蚊蟲叮咬之腫、癢〈4〉抗氧化能力極強。用食鹽水可萃取出miraculin 這種醣蛋白，經由生化實驗，推測使酸味變甜為其cover 舌尖甜味味蕾之結果，分子量約為40000 左右；但在檢測過程中，發現對咖啡、黃連和肉桂，都沒有太顯著的效果，只有酸味有顯著的改變，和以往所閱讀的研究報告有出入，因此懷 疑有氧化還原等其他化學效果，將再做深一層研究。消除蚊蟲叮咬之腫癢的成分確定為小分子所致。經由Prolox 當量測定法檢測神秘果抗氧化能力數值高達4974g/nmol，比一般中草藥及蔬菜多3000 左右。使酸變甜的原因若深入研究對糖尿病患者和減肥者都是一大福音，塗抹蚊蟲叮咬藥膏也可用天然物質製作，而抗氧化能力高更對人體健康有所幫助。當台灣已大量栽植，相對於日本及美國因地寒而無法培育成功，神秘果研究可成為另一項產業發展契機。 "Miracle fruit” is a fruit from West Africa. Though it's not sweet itself, if you eat anything that is sour or bitter after eating miracle fruit, the taste will turn sweet. After researching further material, we discovered that there are many amazing functions in miracle fruit, and decided to pick up some of which to study. (1) Turning the sour and bitter tastes into sweetness (2) Relieving alcohol (3) Relieving the hurt from mosquitoes and bugs (4) An excellent antioxidant. We can extract the miraculin that changes the taste from NaCl (aq), and through the biological experiment, we guess that's because miraculin covers the sweet sensor. The molecular weight of miraculin is about 40000.According to the experiment, we found out that miraculin doesn't have a great effect on the taste other than sourness, such as the bitterness of black coffee, Coptis chinensis, and cinnamon. . It is much more different from the former report we read. So we doubt that there are some other reactions. The thing, which relieves the hurt from mosquitoes and bugs, are sure to be a simple molecule, not a protein. By the Prolox equivalent weight experiment, we found that the ability of antioxidation got to 4974g/nmol, which is much higher than the normal vegetables and fruits. The effect of taste changing is really good news for diabetics and weight reducers. And the medicine can also be made by natural material. The excellent antioxidation is helpful for our health, too. Since Japan and America cannot grow the miracle fruit because of the cold weather, developing the functions of miracle fruit seems to be another chance for Taiwan to stand out in the world.