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外來植物的入侵-非洲大鳳仙的生態探討
Generally speaking in order for a exotic plant life to dominate over the local ecology, the following factors must hold true: 1. A close proximity of the environment (including geographical and climatic factors) 2. A lack of nature enemy and competitor 3. A strong reproductive and adaptability capacity. Base on the above factors, We will be using Field Observation record, Sample placement, virtual environment factor experiment and other methods to conducing our investigation. This study shown the African Touch-Me-Not(Impatiens wallerana) has a strong reproductive and adaptability capacity. Even under adverse condition the African Touch-Me-Not survive for a period of time. It can produce flowers then fruits all year round, within each fruit there is on average 45 seeds with, under the right condition, near 100% germination rate. It can also reproduce asexually via cutting. Taiwan’s warm and humid geographical condition is similar to its origin of Africa. And so far haven’t found any obvious natural enemy. In the future this plant will be likely to spread on the low to mid. level mountain region. It also can be seen that the divergence within its habitat is low. Therefore it will decrease the variety, the density and the diversification of the local eco-system. Here to advise the government’s forestry and agriculture department, to study the relevant ecological information on this species, when they are considering popularization this plant. Using the information to evaluate the impact of this plant may have on the environment, and use it as a guideline for their plant conservation policy. 一般而言,外來植物入侵本地原生植群成功的條件有:一、相似的環境(包括地理、氣候等因素)。二、缺乏天敵及競爭對手。三、旺盛的繁殖力和適應力。針對上述條件,利用野外觀察記錄、樣區設置及模擬環境因子實驗等方法,來探討非洲大鳳仙未來是否會在臺灣蔓延、擴展進而影響本土的生態系。結果顯示非洲大鳳仙有旺盛的繁殖力和適應力,?使在不利的環境下,短時間不會死亡。一年四季皆可開花結果,每粒果實平均有四十五粒種子。環境適宜時,有接近百分之百的萌發率,亦可用莖進行無性繁殖。另外,臺灣溫暖多雨的地理環境亦與其原產地非洲相似,且未發現明顯天敵,未來極有可能在中低海拔山區蔓延。調查也發現它生長的地區岐異度明顯偏低,使其他植物種類、密度減少,生物多樣性降低。因此建議政府、森林、園藝和農業工作人員在推廣時,多吸取相關物種的生態學資訊,評估其對環境可能造成之影響,以作為植物保育之重要參考。
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Equtatetor-新一代智慧型數學處理器
此研究的目的是要設計出一套完整編輯顯現數學式、加以計算,並求出解的一套方法與成品。而這項工作的執行者,在此稱之Equatetor 。一般的數學式子,若要計算的話,普通的計算機是不足夠的。原因是它們沒有辦法表現出數學式的「原貌」,例如分號、指數、函數、根號等數學符號混在一起時的情況。於是,我便擬定了一個研究,希望設計出一套更方便且實用的方法。換句話說,我要設計出一個功能強大的工程計算機程式。其中,自然牽扯到數學式子的顯現方式(以MathML 實現),以及計算機科學的演算法及資料結構。我主要的目的有四:(1) 顯示數學式(2) 方便編輯數學式(3) 計算數學式(4) 處理可以以不同形式輸出解答的計算(如輸出分數、根號、函數解等)。研究結果中,成功地運用XML 中的MathML 與二分逼近分數等演算法及若干資料結構,達到了以下實用的幾點:(1) 結構化的數學式編輯(2) 完整地顯示數學式(3) 正確運算並輸出運算式的答案(4) 提供一般數學形式之解(非小數之解);The object of this study is to design a method and processor which is able to edit, display a mathematical expression representing a number, calculate and output the answer. The executor of this task is called Equatetor. Normal calculators are not adequate for this kind of task. The main reason is that they can’t reveal the original expression, such as fractions, radicals, exponents or mathematic functions. Therefore, a simple and convenient method is needed. To perform the possible way of handling those tasks, a computer program has been written. Several techniques were used, such as MathML, computing algorithms, data structures, and so on. Following are main purposes: (1) Displaying mathematical expressions. (2) Editing mathematical expressions simply. (3) Calculating mathematical expressions. (4) Outputting the answers(in different expressions). And the achievements:(1) Structured methods of editing of mathematical expressions. (2) Displaying mathematical expressions completely. (3) Calculating mathematical expressions precisely. (4) Offering answers in different expressions.
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抑制水果黑色素形成之新理論及研發美白保養品之新概念
PPO 是一種含銅的多酚氧化? (E. C. 1. 14. 18. 1),主要是將酚類 (phenol) 氧化成二酚類(diphenol),更近一步的變成quinones,後者是一種不溶於水的褐色聚合物,在植物中造成褐化最主要原因,然而在人類皮膚則產生黑色素 (melanin)。我們提出有一種揮發性的抑制劑存在動植物體內,此種抑制劑會抑制生物體內PPO 的活性。植物在收成後,揮發性的抑制劑逐漸消失,導致內生性的PPO 活性逐漸上升,因此植物便會產生深褐色的斑點。在本研究中,我們以玉蘭花作為例子,以生化酵素動力實驗、部分純化黑色素抑制劑來解釋揮發性抑制劑在植物體內的存在及其作用,更進一步對水果快速褐化提出一種新的理論。研究發現PPO 的褐化反應就像是人體內酪氨酸? (tyrosinase) 的催化反應,酪氨酸?可以使人體產生黑色素而累積在皮膚上形成黑斑,利用〝人工皮膚〞模擬揮發性黑色素抑制物的作用,證明,防止PPO 抑制劑之揮發可能在未來美白保養品工業裡扮演一極具潛力的角色。Polyphenol oxidase (PPO) or tyrosinase (E.C. 1.14.18.1) is an important and ubiquitous enzyme responsible for browning in plants and melanization in animals. PPO is a copper-containing enzyme that catalyzes the chain-oxidation from monophenol or polyphenols to o-diphenols and subsequent o-quinones. The resulting quinones are large wate-insoluble polymers with dark brown color. We proposed that volatile inhibitors are associated with the plant PPO and block the PPO activity in vivo. While post-harvesting the volatile inhibitors evaporate, the endogenous PPO is then activated and therefore instantly produces dark quinone pigment. In the present study using magnolia flowers as an example, we show the presence of a potent volatile inhibitor(s) for PPO in plant. The novel finding clarifies the mechanism involved in the browning phenomenon of post-harvesting for most fruits. Since the PPO is also know present in human as tyrosinase responsible for the formation of “darkening spots” on skin, the finding of evaporation of potent PPO inhibitor may be potentially used as a strategy in developing a novel cosmetic product.
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Investigation of phytoremediative ability of macrophytes and a design of a phytofiltration system for Singapore’s waterways
"Nitrates and phosphates cause eutrophication when present in high concentrations.\r This project aims to employ macrophytes to reduce such macronutrients in water bodies via growth and kinetic studies, which is a unique fusion of methodologies. It also involves a novel design and analysis of several enclosure prototypes to introduce macrophytes into waterways and their effects on the waterway’s ability to convey storm water rapidly away from flood-prone areas.\r Tropical macrophytes (emergent macrophytes Typha angustifolia and Cyperus haspan, submerged macrophytes Hydrilla verticillata and Cabomba aquatica, floating macrophytes Lemna minor) were grown in simulated wastewater with high nitrate and phosphate concentrations. Analysis of the growth and uptake kinetics of the macrophytes showed a correlation between high growth rate and high nitrates and phosphates uptake. C. aquatica had the highest uptake rate for nitrates and phosphates as well as the highest growth rate of 6.11 ± 1.2 % day-1 . The remaining macrophytes were proven to exhibit good phytoremediative properties, with emergent macrophytes C. haspan and T. angustifolia having great affinity (as indicated by a low Km value) for phosphate and nitrate respectively. An analysis of the phytoremediative abilities of each macrophyte was done to provide recommendations for growth in different aquatic areas.\r A total of eight nettings, made from different materials-linen and plastic, and pore sizes were used to design the enclosure prototypes. These enclosures possess a metal skeletal structure for greater stability. Results show that a combination of 2 different nettings provided the best trade-off between ensuring that the macrophytes were contained within the enclosures and minimising the enclosure’s impact on the flow velocity of the waterways. Enclosures could then be attached to existing infrastructure like the float booms as a platform for large scale phytoremediation locally."
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近年來重金屬污染問題日益嚴重,因此檢測地下水、工業廢水中的重金屬含量為一重要課題。本實驗即是利用錄音機中的零件-石英振盪器作為電化學分析偵測的工作電極,在0.1 M KNO3(pH = 3)溶液中利用循環伏安法以50 mV/s 的掃描速度從 – 100 到 – 700 mV 持續掃描 10 圈,來穩定電極表面。
以方波陽極剝除伏安法(SWASV)模式設定預濃縮電位– 700 mV、預濃縮時間60 秒偵測重金屬的鉛離子。掃描電位範圍由 – 700 到 – 100 mV vs. Ag/AgCl 往氧化方向掃描。偵測鉛離子濃度範圍為200-600 ppb,之後可針對各種條件作最佳化的條件探討可降低偵測濃度及實際運用於地下水及工業廢水的檢測。
Heavy metal pollution becomes very serious. Lead (Pb) determination is a continuous research interest in clinical, environmental, and industrial application. The main causes for living biological media are mutagenic, carcinogenic, and teratogenic activity. The determination of trace metal in the ground and industrial waste water will be a important project. Here, we report a simple system which using quartz crystal microbalance (QCM) (use in radio machine) for working electrode. The QCM was first equilibrated in 0.1 M KNO3(pH = 3)base electrolyte for about 1 min before electrochemical experiments. It was then pretreated by continuous scans in the window from – 100 to – 700 mV at a scan rate of 50 mV/s until a stable background current obtained.
The amount of Pb2+ was detected quantitatively using square-wave anodic stripping voltammetry(SWASV). The potential range was set from – 100 to – 700 mV in the cathodic direction for most cases. The standard addition method concentration range is from 200 to 600 ppb. Analytical conditions were systematically optimized and extended to practical applications aslo.
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以阻抗匹配調整太陽能最大功率輸出之研究
In recent years, the price of the oil keeps rising continuously. As a result, the prices of the commodities are rising, too. But what does this mean? This situation stands for the resources on are becoming more and more valuable. A few months ago, I read a Weekly Business Magazine and a Scientist Magazine. They both pointed out that the resources such as oil would disappear after fifty years, and that was a horrible phenomenon. Since almost everything in our modern lives are related to oil, like automobile, motorcycle and air plane. They all need oil for its ingredient. Even plastic bag plays a part in the products of it. I really cannot imagine what it would be like if we don’t have oil after fifty years. Owing to the green house effect is becoming more severe, there are many substitution resources found, such as hydraulic power, wind power and solar energy…etc. What we’re discussing in our topic is how to enhance the power of solar energy, because for now, we all know that the solar module is very expensive and it cost a great deal of money just only one square meter, but the price of module converted from sunlight or heat energy can’t be higher. Therefore, the work is mainly to design and carry out a solar max power point track. 近年來,油價不斷的上漲,連帶著民生的物價漲幅也是越來越可觀,但…這意味著什麼 呢??這所代表的是地球上的資源可以說是越來越珍貴了,前陣子曾經看過商業週刊、科學人 雜誌…他們紛紛所指石油這種能源可能在五十年後就消失殆盡,這個可是非常可怕的結果。 鑑於溫室效應愈趨嚴重,許多的替代能源紛紛出籠,像是風力、水力和太陽能等等。我們這 次的主題是在討論如何提高太陽能效率,現在的太陽能模組我們都曉得非常的昂貴,但是由 太陽轉化成電能的效率卻是低的可以。所以本作品主要目的在於設計與實現一個太陽能最大 功率追蹤器。
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口蹄疫病毒鞘蛋白rVP1 誘發Prohibitin 之遷移
細胞凋亡具有控制生物體細胞數目之功能,能讓特定的細胞走向死亡,因此若能掌握其作用機制,便可能藉由調控細胞凋亡的發生,進而應用於癌症治療。前人研究(2.)指出,經基因重組技術純化之口蹄疫病毒鞘蛋白rVP1,會導致BHK-21的Akt 蛋白質去活化,引起細胞凋亡的現象。然而在其後續的研究中,卻發現到在BHK-21中大量表現磷酸化的Akt 蛋白質,並無法反轉由rVP1 所誘發之細胞凋亡。因此本實驗利用二維蛋白質電泳,尋找Akt pathway 以外之細胞凋亡相關蛋白質。目前已證明Prohibitin 此一蛋白質,在由rVP1 所引起之細胞凋亡中,有自細胞核移動至細胞質的現象。此外,亦經由實驗排除Prohibitin 位於Akt pathway 的可能性。Western Blot 之結果更顯示,經rVP1 處理後,Prohibitin 在很短的時間內便出現遷移的現象,故推測其具有調控細胞凋亡上游反應的功能。Apoptosis can lead some specific cells to programmed death, thus, it is a major way for creatures to control their cells amounts. If we can command the mechanism of apoptosis, we may use it as a therapy for cancer by artificial regulation of apoptosis. VP1 is one of the capsid proteins of Foot and Mouth Disease Virus (FMDV). A research (2.) has indicated that the recombinant VP1 (rVP1) can result in dephosphorylation of Akt in BHK-21, and then lead the cells to apoptosis. However, in their follow-up experiments, they discovered that even if they expressed great amount of phospho-Akt in BHK-21, it still couldn’t reverse the apoptosis induced by rVP1. Therefore, this experiment takes the advantage of two-dimension protein electrophoresis (2D) in order to find apoptotic proteins excluded from the Akt pathway. I have found that Prohibitin exports from nucleus to cytosol after rVP1 treatment. Furthermore, I eliminate the possibility that Prohibitin’s may be located in Akt pathway. The results of Western Blot also shows that protein amount of Prohibitin in BHK-21 increase after rVP1 treatment, hence the purpose of nuclear export of Prohibitin might not be to degrade it. It might have some much more important function in the process of exportation. Besides, Prohibitin exports to cytosol in quite a short time after rVP1 treatment. According to this phenomenon, I suppose that Prohibitin has a role as a regulator of apoptotic up-stream reactions.
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「渦」藏「聖」機--以渦流脫離重新詮釋聖嬰發生的原因
If we place a block in continuous, steadily flowing water, some periodic eddy currents will appear behind it. In my report, I would like to introduce a new way to explain how the periodical movement of eddy currents would help to triggering El Nino. According to my experiment, these eddy currents have a certain life cycle, and the eddy current give a force, which drag water form the area they just went by. It will cause water level became lowest for a period of time. These phenomena are also shown in the real data of “Sea Surface Height Anomaly”, it is a quiescence period, and then changes into the highest one it is interesting that highest one always follows by the starting of El Nino. Another important result from my experiment is that, in the same fluid, the frequency of eddy currents is controlled by 2 variables: Size of the block and velocity of the flow, From this result, then get the frequency of eddy current-occur every 3.2 years. It is just correspond with frequency of historical data the El Nino from the 70’s to the present.
本報告嘗試以南極繞極環流在南美洲南端產生週期性渦流脫離的現象來解釋聖嬰的發生根據水槽實驗的結果,水流在通過障礙物後渦流脫離瞬間,會形成一水位低的空區,之後在周圍的水大量湧入,水位突然升高,並有一段時間的振盪。另外,實驗結果也顯示,渦流形成和脫離頻率大致上和注水流速成正比,且和障礙物的大小成反比。根據此結果,對照實驗結果,我認為1993 年3-4 月,1996 年1-6 月及2001 年7-10 月三個「海平面高度異常」的最低值密集帶,此極可能是渦流脫離的時段。且對照「海平面高度異常」實際資料後,發現「海平面高度異常」的「最低值密集帶」後均伴隨水面「寧靜期」,接著就出現周圍暖水流大量湧入的「最高值密集帶」,此時南美洲東南側海水溫度驟增,接著馬上接續著聖嬰的到來。「海平面高度異常」分析的結果,指示渦流脫離的「最低值密集帶」和顯示著聖嬰到來的「最高密值密集帶」間約間隔半年左右。我們推算水的史托爾數約為1,以這個值代入實際南極繞極環流通過南美洲南端,得到渦流脫離的頻率約每3.2 年一次,和長期聖嬰發生的平均頻率非常接近。
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湖光山色下的哀愁--由漂浮的琵琶鼠魚探討外來入侵種與放生行為
本研究自2005 年12 月開始,以臺北市內湖區大湖自公園死亡的漂浮琵琶鼠魚(Many-rayed Sailfin Sucker Catfish )為主要研究對象,探討外來物種與放生行為對大湖生態系的影響。 研究期間共觀察到死亡的琵琶鼠魚隻數計有1085 隻,可記錄到個體形質資料的隻數為910 隻,背鰭棘數則有11 棘、12 棘、13 棘與14 棘四種,分析四種不同棘數的琵琶鼠魚後發現:在體長、頭寬及吻到背鰭長度均無差異(p>0.05),因此判斷所記錄的個體應是棘甲鯰科(Loricariidae) Pterygoplichthys 屬中的同一種魚種。由檢視魚體並分析記錄數量與環境因子相關性後推測:琵琶鼠魚死亡主因是劇烈起伏的溫度差異,其次是人為因素的刻意傷害。 所記錄到大湖池塘水棲動物有:環節動物、軟體動物、節肢動物、魚類、兩棲動物及爬行動物等六大類共34 種,其中外來物種有16 種,本地入侵物種有3 種,而這些外來動物進入大湖的主要管道應是由個人的棄養或放生行為所造成。琵琶鼠魚因繁殖能力強、具攻擊性、吞食其他魚種卵塊、可適應高污染水體及垂釣客對魚種的篩選等因素,而成為最具生存競爭能力的優勢魚種。不但造成大湖池塘呈現嚴重魚種單一化,更可由靜止水域大量擴散至流動水域。未來如何將政府相關部門、學術研究單位與民間社團等力量結合,共同防範大湖琵琶鼠魚的持續蔓延、宣導民眾正確的放生觀念、積極改善大湖水體污染狀況,並訂定完整之外來物種移除計畫,以期恢復大湖池塘物種歧異度,都將是刻不容緩的重要生態課題。;This research began in December of 2005. Focusing on dead floating Many-rayed Sailfin Sucker Catfish in Dahu Park, Nei-hu Distrct, Taipei city. We discuss the impacts of Alien and of behaviors of the release of captured animals on Dahu Park’s ecosystem. During observation period, there were totally 1085 dead Many-rayed Sailfin Sucker Catfish, of which 910 bodies were found. The number of the thorn bushes on dorsal fins includes 11, 12, 13,and 14. After analyzing four kinds of different thorn bushes, we find that no differences exist in Total length, Head width, Predorsal length (p>0.05). We hence conclude that the recorded object should be species with identical with Loricariidae Pterygoplichthys. With the inspection of the fish’s body and the analysis of the relevance of the recorded quantity and the environmental factor, we infer that the main reason of the Many-rayed Sailfin Sucker Catfish’s death is violent temperature differences and the second is attributed to human’s intentional abuse. The aquatic animals of Dahu Park amount to 34 kinds and six classes such as Annelida, Mollusca, Arthropoda, Pisces, Amphobian, and Reptilia. Among them, 16 kinds that are Alien and 3 kinds are Native Invasive Species. Intentional abandon and release behavior channel of these Alien to Dahu Park. Many-rayed Sailfin Sucker Catfish are highly productive, aggressive, and adaptable to highly polluted water. Besides, they eat ovums from other fishes. Moreover, they are not the fisher’s preference and hence often thrown back into pond once hooked . Thus, they become the most competitive survival fish species in Dahu Park. The high competitive ability of Many-rayed Sailfin Sucker Catfish causes Dahu Park to present a serious unification of fish species. This serious unification of fish species could also be spread from static water areas of Dahu Park to flowing water areas. According to our research, certain urgent ecological issues in Dahu Park are to take precautions against the spread of Many-rayed Sailfin Sucker Catfish, to promote the correct idea of releasing captured animals, to improve Dahu Park’s water pollution, and to stipulate a complete plan about eliminating Alien. Our research suggest that government’s relevant departments, academic research units, and folk corporations should be cooperated to achieve the above four goals. Once the four goals are achieved, we believe that the fish species of Dahu Park will be full of varieties again.
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高分子發光二極體乃是利用電子和電洞在發光工作層結合而形成激子,激子在形成後隨即以光的形式衰退,而發出光來。一般而言,高分子發光二極體是一種三明治的形式,電子注入層/發光工作層(高分子)/電動注入層,而通常我們會選擇加入電子傳遞層和電動傳遞層,來增加其發光效果。本研究係針對聚苯胺做為電洞傳遞層對發光二極體之影響,分別對有電洞傳遞曾和無電洞傳遞層之發光二極體作導電度測量、循環伏安法、電位-電流測量、亮度-電位測量。發光二極體中所須之高分子層,須利用旋轉塗佈機,將其均勻的披覆在載體上,由於本校並無此儀器,所以將離心機改造成簡易旋轉塗佈機,並觀察其披附之效果。另外,在封裝元件時,係因學校缺乏真空蒸鍍的儀器,所以將利用電鍍法把鋅鍍在ITO玻璃上,或將鋁片和ITO玻璃緊靠在一起,針對此二替代方法,本研究將探討其所製出原件成效。Polymer light emitting diode (PLED) utilizes the energy gap between the layer of electron and electric hole that emits the light due to the decay of solitron. PLED is always in the sandwich from, that means the conducting polymer is contained between metal as an cathode and indium tin oxide as an anode. It is used the addition of another electric transfer layer and electric hole transfer layer to improve the lighting efficiency of PLED. The purposes of this study are to discuss the effect of polyaniline as the electric hole transfer layer. The study methods are conductivity measure, the cyclic voltametry, the luminance-voltage curve. The simplified spin coating machine was designed to coat the polymer onto the ITO. The zinc was electroplated onto the graphite as an cathode.
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Extreme Mouse Mobile
Objective To design build and run 5 Mouse mobiles – vehicles which use mousetraps as their only source of energy. Targets- • Go up a ramp • Raise a flag • Hit something and set it free • Deliver something • Catapult Details These mouse mobiles are original creations which include some commercially produced parts. They are powered by single spring driven standard wooden mousetraps. They are 5 in number each being used for the respective tasks. Basic structure Each mousetrap consists of a 2 parallel wooden planks which has 2 holes drilled on either sides for the rear and front axles. A rod is used as the axle and CD disks are used as wheels. The mousetrap is attached to the planks. Movement and Locomotion To move the mouse mobile a string is connected to the rear axle. The string runs through 2 pulleys which change the direction of the applied force and are attached to the hammer of the spring. When the vehicle is pulled backwards on the floor tension is created in the string (as the hammer is stretched) which gets stored in the form of spring energy. When the wheels are released on the floor the rear axle starts rotating hence propelling the mouse mobile forward. Completion of objectives 1) To go up a ramp For the required task a high amount of torque would be needed .That will be sufficiently provided by the spring energy. 2) Raise a flag For the required task a rod is placed on the wooden platform. The flag is placed at the bottom of the rod; it is connected to the hammer of the mousetrap using a string. The spring and the rod are placed in the opposite directions. When the hammer is released after being stretched it pulls the string and raises the flag. 3) Deliver something For the required task 2 mouse mobiles are used. A platform of any hard and light material is attached to the rear of a mouse mobile. After reaching the desired location, when the hammer of the primary spring is fully relaxed it hits the catch of another stationary mouse mobile. The hammer of the respective catch is connected to the platform using a string. Upon activation the hammer is released, the platform is pulled with extremely high speed and the materials on the platform, due to their moment of inertia fall down. 4) Hit something and set it free. For the required task 2 mouse mobiles are used. A small mousetrap is attached to the front of the mouse mobile which is in contact to the required object. The other mouse mobile on reaching the above vehicle hits its catch using its hammer. The hammer of the stationary mouse mobile is released and the object is hit and set free. 5) Catapult For the required task 2 mouse mobiles are used. A mousetrap is attached to the front of stationary mouse mobile which is attached to a strainer and the object to be catapulted is placed inside it .Another mouse mobile approaches is and hits the catch hence activating the hammer and the object is catapulted.
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Biosolids : Bio-transfer Factors of Trace Metals
With an increase in the use of biosolids as a soil replacement and as a fertilizer for crop production, consumers continue to question the safety of crops grown in biosolids.Although past research has shown that there are no adverse affectsin the nutritional value of plants grown in biosolids, I furtherresearched the safety levels of trace metals in crops fertilized using biosolids.
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