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「蓮」「環」密碼--環境因子對蓮花效應的影響
蓮花效應是指蓮葉表面具有奈米纖毛結構,因此只要葉面稍微傾斜,水珠就會滾離葉面,在我們生活週遭,許多植物具有蓮花效應。本實驗選擇彩葉山漆莖作為研究材料,因為我們發現在同一植株上,嫩葉的蓮花效應最佳,而老葉幾乎無蓮花效應。當彩葉山漆莖的新葉轉為老葉,蓮花效應會減弱,甚至消失。我們以不同水量、土壤酸鹼值及光照作為變因,來探討蓮花效應改變的原因,結果發現水量並非主要影響蓮花效應改變的變因;土壤過酸或過鹼,會減弱新葉及嫩葉的蓮花效應;置於暗室則使整株彩葉山漆莖所有葉面皆無蓮花效應。許多植物的性狀,在老化或面臨環境改變時,會將控制性狀的基因開啟或關閉。因此,我們推論,當環境因子改變時,植物的蓮花效應可能是經由基因層次的調控,藉以增強或減弱此性狀的表現。如果不是基因的開啟或關閉,則有可能僅是葉表面的結構發生些微的改變,真正詳細的機制仍有待進一步的確認。;We choose Breynia nivosa (Bull ex W. G. Smith) Small as a model plant to study the lotus effect on the leaves for the reason that on the same chosen plant the new-born leaves have the best lotus effect while the elder ones have little lotus effect. When the new leaves turns into elder ones, the lotus effect also turns weaker or even vanishes. To explore the exact mechanisms, we take water quantity、soil pH、and light density as the experimental factors. The results show that water quantity cannot affect the lotus effect on all leaves, change in soil pH can decrease the lotus effect on the new and new-born leaves, and dark treatments can eliminate the lotus effect on all leaves. When the environments change, the phenotypes of plants could also be changed to adapt to the new conditions by turning on or off genes. Therefore, we suggest that the lotus effect on the leaves is also controlled by genes to increase or decrease its phentype so as to adapt to the changing environments. If not, it may simply be a little change of the surface structure of the leaves. The detailed mechanism remains to be confirmed further.
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Detection Device for Alcoholic Drunk Persons
The purpose of this project was to create a detection device for alcoholic drunk condition in human by using the principle of vapor pressure difference between breath samplings from normal and alcoholic-drunk men. The work comprises of three major steps. The first task was an experiment to determine an average air volume that can be fully blown out from the lungs of non-drunk people as a control. Twenty adult Thai volunteers weighing between 50 - 80 kg (average 59 kg) were used. The average blown out air volume was found to be 369.9 mL, with the range in values from 340-400 mL (sample size N = 20, SD = 15.47). The second task was an experiment to measure relationship between the blown air volume (100-700 mL, both from alcoholic-drunk and control groups of people) and the resulted vapor pressure by using manometer. The vapor pressure of normal breath increased from 400 to 1,600 newton/m2 with increasing blown air volume, whereas that of the alcoholic-drunk was found to be 600 to 1,800 newton/m2. The last task was to create a detection device prototype to gauge the alcoholic content in the human body from the breath. Air volume of 300 mL was arbitrary chosen to trigger lighting up of indicator lamps. The breath samples of low vapor pressure (low amount of alcohol, 21.12-44.00 mL, equivalent to 14.00-29.17 mg%) would trigger a green lamp to light up. A moderate vapor pressure range (medium amount of alcohol, 88-132 mL, equivalent to 58.33-87.57 mg%) would trigger green and yellow lamps to light up while a high vapor pressure (high amount of alcohol, 250 mL, equivalent to 165.72 mg%) would trigger green, yellow and red lamps to all light up. None of the three lamps would light up from (non-alcoholic) breath of control people. This device has also been tested to external group of volunteers. The work in this project has successfully demonstrated a useful application of simple principle in chemistry on partial vapor pressure.
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吸〝氣〞大法-QCM 對有機氣體之吸附與偵測
中文摘要:\r 化學實驗中,常常會使用到各種具有揮發性的有機溶劑,如醇類、酸類、醛類及酮類等。\r 當吸入過量的揮發性有機溶劑時,將會對人體造成嚴重的傷害,故使用時格外需要留意。有\r 鑑於此,我們開始著手研究如何偵測氣相中的有機氣體分子。\r 本研究中,我們利用聚苯胺薄膜吸附氣體分子的特性並結合對質量變化極靈敏的石英晶\r 體微天平,自行設計了一套簡單、藥品用量少且不需昂貴儀器的實驗系統。藉由此系統,我\r 們有效的針對各種揮發性有機溶劑進行偵測,並進一步地探討不同氣體分子對於聚苯胺薄膜\r 的吸附現象。\r \r 英文摘要:\r Students usually use volatile organic solvent in laboratorys such as alcohols, carboxylic acids,\r aldehydes, ketones, etc. It will injure our body when we breathe in too much volatile organic\r solvent. For this purpose, we begin to study how to detect volatile organic compound in air.\r In this work, we combine polyaniline membrane which can adsorb gas molecules and quartz\r crystal microbalance which is highly sensitive to mass changes to design a simple and inexpensive\r system which needs only little chemicals. With this system, we can effectively detect different\r volatile organic compounds. Furthermore, we can also study the adsorption of polyaniline\r membrance for different volatile organic compounds.
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Quantitative Analysis of Organism Growth Using Fractal Dimension Statistics
Cultures of bacteria were analyzed using fractal geometry and statistics to provide a method for predicting organism growth, paving the way for a better design of treatment drugs. Images of three cultures of isolated Bacillus subtilis were taken at time intervals of two to three hours for eight days. The images were processed using the IDOLON program and quantitatively described using three statistical formulas: fractal dimension D, Renyi dimension and Hausdorff-Besicovitch dimension. The three variables were integrated to compute the maximum of the distribution and were used as coordinates for a 3-dimensional graph f. A 2-dimensional graph g containing the maximum of a distribution under time analysis was also constructed. Topological properties of the graphs, including slope, direction and area were used to determine the interrelationship of the three fractal values. The two graphs, described as φ - : X -? P1 where X is the smooth algebraic assimilation of the four variables under time analysis, was extended using Java. A computer-aided prediction model of the graphs f and g were made which combined the topology of f and g at infinity. The computed fractal values showed the existence of a fractal pattern in the growth of Bacillus subtilis with fractal dimension ranging from 0.900 to 4.000, indicating a linear iteration. This was supported by the values of the Renyi dimension, which showed a horizontal growth pattern of the bacterial cultures, establishing the growth of the bacteria to be inclined to go towards the North East direction. There was consistency in the computed fractal values, maximum of distribution and topographical computations of all three cultures which also indicated the existence of a pattern of growth which could be extended to tinfinity, thereby allowing prediction of the direction and rate of growth of the bacterial colonies. The fractal patterns in the growth of bacteria, in this case Bacillus subtilis, yielded the direction and rate of growth of the bacteria as shown by the analysis of the fractal patterns and statistical values, showing that the growth of harmful organisms can therefore be predicted, making it possible to improve on the design of drugs for the control of perilous cells. By preventing the growth of insidious cells, the potential effects of virulent organisms may be avoided, and treatment may be made more possible.
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本研究主要的目的是在開發二氧化碳製造、過濾與循環的整合型水族養殖系統。二氧化碳的產製是以基於發酵原理,並搭配具有純化二氧化碳作用的水濾裝置,發酵環境溫度以水族箱內的水來維持,循環過程中並搭配外部過濾器,所有容器均由保特瓶改裝完成,整合成一套價廉、環保且具有高效率的水族養殖系統。由實驗結果可得知,二砂糖發酵對於二氧化碳產生率而言,其最佳發酵環境溫度範圍25℃~35℃,一般水族箱的水溫即可保持在此一溫度範圍。過濾系統則使用光電比色計與一般市售過濾器相比較,亦有令人滿意的效果。本實驗除了研究以二砂糖發酵之外,還希望更進一步地朝向廚餘減量方面開發其他發酵材料,希望能為環保盡一份心力。;The purpose of this study is to develop an integrated system of aquatic breeding with the functions of producing carbon dioxide, filtering and circulating, where carbon dioxide is produced based on fermentative theorem and purified by the water filtering; the environmental temperature of fermentation is maintained by the water in the aquarium and circulating is through an external filter. All the containers are made of PET bottles and thus integrated a cheap, environmentally friendly and high efficient system of aquatic breeding. According to the experimental result, for the producing rate of carbon dioxide, the best fermentative temperature of NO.2 granulated sugar is 25℃~35℃. With a photocell colorimeter to compare the function of the self-made filter with that of the filters on the market, one can find it’s comparable. In addition to the fermentation of NO.2 granulated sugar, the study is also expected to develop other fermentative materials to reduce kitchen waste for environmental protection.
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在網際網路蓬勃發展的時代,電腦病毒也更加的猖獗。雖說防毒軟體百家爭鳴,但對於未知病毒的防治技術仍然束手無策。本研究的目的在追溯出電腦惡意程式運行模式,利用IDA靜態反編譯軟體與病毒原始碼尋找出病毒共同的特徵,阻斷其滋生途徑。 本研究在實驗中,研究者利用Borland C++ Builder 6作為應用程式開發環境,配合WinAPI函數與TRegistry類別設計出參數監控系統。研究顯示不論是修改於Window 9x架構下的win.ini, system.ini,或是Run與Run Service之下的資料項,本系統都能有效運作。此系統能成功的攔截到惡意程式對Registry鍵值的讀寫,亦即當使用者否決其修改鍵值時,惡意程式無法在重新開機後運行;易言之,惡意程式失去執行效力。 實驗結果發現,參數監控系統可攔截惡意程式之修改命令,防止未知病毒與惡意程式開機自動運行,以此達到未知病毒的攔截基礎技術。本研究之結果可補強市售防毒系統,對於未知病毒的攔截,能達到完整的防護效果。 Computer viruses become more rampant in the rapidly-developing era of Internet. With the various kinds of Anti-Virus software, people still can do nothing about detecting the unknown viruses. This research aims to trace back the computer virus function mode. By using IDA Pro Disassembler and Virus Source, we can find out the common characteristics of viruses. Thus, we can stop viruses from thriving. The experiment uses Borland C++ Builder 6 as the Registry Detection System by coordinating Win API and TRegistry. It works no matter by modifying win.ini and system.ini under the structure of Window 9x or by modifying Run Value and RunService Value. Moreover, it can intercept the viruses from reading and writing of the ValueKey. As the users deny the modified ValueKey, the viruses cannot run after resetting. In other words, the viruses lose their effects. The results present that Registry Detection System can intercept the modifying commands and prevent the automatic running from unknown viruses. By doing so, we can acquire the basic technology of intercepting the unknown viruses. The application of this study can improve the functions of Anti-Virus. In this way, through protection from unknown viruses can be obtained.
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A Novel Procedure to Identify Genes involved in Electron Transfer of Exoelectrogens
Purpose of research. Microbial fuel cells (MFCs) are bioelectrochemical systems that generate electrical energy by exploiting the extracellular electron transport (EET) capabilities of electrochemically active bacteria (EAB) (Logan 2009). This investigation aims to identify genes involved in driving bacterial EET with a new procedure that enables rapid screening of a side array of genes. These insights may lead to improved MFC performance through enhancing reactor design or genetic engineering EABs (Alfonta 2009). Procedures. MFC metagenomic analysis. Twelve MFCs incubated with four different bacterial samples were operated for approximately one year. The bacterial DNA from before and after incubation was extracted and the 16S rRNA regions were PCR amplified and sequenced. The bacterial community changes were analyzed using the QIIME program to identify bacteria that were being selected. Fosmid Clone Isolation. An E. coli fosmid library (Mewis et al. 2013) that contained genes from EAB inferred in the previous step was incubated in three MFCs. After a 48 hour enrichment period, biofilm samples from the MFCs were extracted and individual clones were isolated and screened in the MFCs individually. An E. coli DH5α strain with no insert DNA was incubated separately as the control. DNA sequencing. Fosmid insert DNA from high-performing clones were extracted, purified using gel electrophoresis, constructed into sequencing libraries and sequenced. Bioinformatics Analysis. The sequences were constructed into larger contigs using the Velvet algorithm package. The open reading frames (ORFs) were inferred and translated into amino acid sequences and annotated with proteins identified from the KEGG, and SEEDs databases using Metapathways 2.5. Results. The changes in bacterial communities from the metagenomic analysis revealed increases in relative abundance in numerous genera from Firmicutes and Bacteroidetes. The MFCs incubated with the fosmid clones generated about 4 times more peak power than the MFCs incubated with the E. coli DH5α. Polarization curves generated for the MFCs demonstrated that the fosmid clones were able to sustain a higher current. Incubation of pure cultures of individual clones yielded four clones with significant performance improvements over the control strain. Protein data from Metapathways outputs reveled both novel and previously reported EET genes encoding for Type IV pilus structures, c-type cytochromes, soluble cytochromes, flavoproteins, and porins. Taxonomy inferences of the gene inserts by the Green Genes database reveal the genes most likely came from the same EABs that were inferred from the metagenomic analysis. Conclusions. The increased performance of the fosmid clone-powered MFCs suggest that the clones carried genes that enhanced their performance in the MFCs. This is further confirmed by polarization curves generated for the MFCs. The results of the taxonomy inferences suggest that the bacteria being selected for in the environmental samples carried genes that enhanced their performance in the MFCs, and that these genes were successfully identified in the subsequent steps. The results of this study demonstrate that using a gain of function approach to rapidly screen a wide array of genes in a gene library may be an efficient method to identify genes that enhance power generation of EABs in MFCs.
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Viable Energy From Ocean Waves
(a) Purpose of Research To investigate the wave conditions offshore along the South African coast to determine wave heights, intervals, and patterns. These results have demonstrated the power potential of ocean waves and identified sites for offshore power stations. The waves off of the South African coast are the most viable, as they have wave heights of between 2.7m (9ft) and 14.6m (48ft). It is also to assist in the development of my power station design, through research into offshore wave composition, principles, periods and characteristics. (b) Procedures I have used various calculations including the surface pressure of salt water per square meter to calculate the potential power produced by a buoy. These figures have been expressed in Kilowatt Hours, and are then able to be divided by the known consumption of a single USA household in 1 year. A figure of the amount of households that can be supplied by a single generator will then be reached. An approximate power output for a single buoy is between 200 and 300 homes per year (Dependant on wave height) A concept for a maintenance free electrical generator suitable for marine use has been investigated. The design will be made as: ● A working demonstration model capable of producing electricity, which consists of a Rotary Induction Generator, ● a scale model to show the appearance of one such generator & ● A large scale model to show how generators can be congregated to form a power station offshore. (c) Data Utilising the calculations of potential power output and the wave data, the financial viability of the generator has been calculated, in relation to current fossil fuel power stations, down to a cost per Kilowatt. Wave data from international marine monitoring websites that provide real time wave condition graphs, have been tracked by myself daily for over 1 month and recorded to provide a large data resource. This provides wave heights of multiple weather systems as well as averages. Costs have been investigated from Internet sources for electrical integration to the national power grid, as well as the generator manufacture. These are estimates, as the exact specifications of my device cannot be finalised without further prototypical research. (d) Conclusion With conclusions reached by thorough research into wave dynamics, weather patterns and their effects on wave heights, Rotary induction power generation and costs related to multiple power systems, I intend to demonstrate fully to the International Electrical Producers, that coal fired power plants are more costly and environmentally damaging than my revolutionary concept for a truly economically viable, ocean based generator system.
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這個專題研究的目的是要發展出一套簡單可靠的方法和廉價自製的器材,在地面上即能有效估測大氣層的厚度。我們小組研究光學中雷氏(Rayleigh)散射的原理,針對空氣分子對光線散射作用和特定方向之偏極效應,利用一已知散射長度之路徑,測量其偏極光的強度,同時比對由大氣層散射而來,在同一偏極面上的散射光強度,即能估算大氣層的厚度,方法簡單新穎,自製器材經實際測量和改進,有發展和推廣的價值。\r The main idea of the experiment is to set a system in order to effectively estimate the thickness of the atmosphere. On the theory of “Rayleigh Scattering” (small air molecules sizing about 10-4μm), we developed an equipment that has two tubes. The tubes lead the scattered lights from two paths. One is called “air light” scattered in the ground air, and the other “sky light” is scattered in the sky and reflected by a beam splitter. The two paths are on the same plane; the scattered lights are perpendicular to the direction of sunlight and 100% polarized. We could adjust and measure the distance “d” of the air light path. We simultaneously observe and compare the intensity of the lights from the two paths with the electronic instrument made by ourselves. By using the known distance “d” and the reflection “x” of the beam splitter, we can calculate the thickness of the atmosphere. The experiment is simple, novel and easy to do in an extensive field at school. Researchers don’t have to use a bloom, radar or satellite to discover the atmosphere, but you could use a simple equipment to observe the features of it.
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The purpose of this project is to create a project that can improve the quality and the production of the merchandise and also to prevent a 100% of mistakes in the electronic components which are complicated to detect or not detectable for us when we are making a circuit. Other of the purpose of this project is to prevent the bad production in the products which are elaborated in a company and then to check if the employee is working well, in that way the company will be able to avoid the bad production and it will be more profit for the company thanks to the products.
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Equtatetor-新一代智慧型數學處理器
此研究的目的是要設計出一套完整編輯顯現數學式、加以計算,並求出解的一套方法與成品。而這項工作的執行者,在此稱之Equatetor 。一般的數學式子,若要計算的話,普通的計算機是不足夠的。原因是它們沒有辦法表現出數學式的「原貌」,例如分號、指數、函數、根號等數學符號混在一起時的情況。於是,我便擬定了一個研究,希望設計出一套更方便且實用的方法。換句話說,我要設計出一個功能強大的工程計算機程式。其中,自然牽扯到數學式子的顯現方式(以MathML 實現),以及計算機科學的演算法及資料結構。我主要的目的有四:(1) 顯示數學式(2) 方便編輯數學式(3) 計算數學式(4) 處理可以以不同形式輸出解答的計算(如輸出分數、根號、函數解等)。研究結果中,成功地運用XML 中的MathML 與二分逼近分數等演算法及若干資料結構,達到了以下實用的幾點:(1) 結構化的數學式編輯(2) 完整地顯示數學式(3) 正確運算並輸出運算式的答案(4) 提供一般數學形式之解(非小數之解);The object of this study is to design a method and processor which is able to edit, display a mathematical expression representing a number, calculate and output the answer. The executor of this task is called Equatetor. Normal calculators are not adequate for this kind of task. The main reason is that they can’t reveal the original expression, such as fractions, radicals, exponents or mathematic functions. Therefore, a simple and convenient method is needed. To perform the possible way of handling those tasks, a computer program has been written. Several techniques were used, such as MathML, computing algorithms, data structures, and so on. Following are main purposes: (1) Displaying mathematical expressions. (2) Editing mathematical expressions simply. (3) Calculating mathematical expressions. (4) Outputting the answers(in different expressions). And the achievements:(1) Structured methods of editing of mathematical expressions. (2) Displaying mathematical expressions completely. (3) Calculating mathematical expressions precisely. (4) Offering answers in different expressions.
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聚對位苯基乙烯高分子發光二極體性能研究
高分子發光二極體為導電高分子之熱門研究課題,因其有低成本、製程簡易、可大面積化之優點為無機高分子發光二極體所缺乏,所以受到學術界與工業界之注目。由於高分子發光二極體其有廣視角、高答應速度、低驅動電壓、可製成撓曲式元件等特質,極具成為下一世代顯示器之潛力。高分子聚苯胺為高分子發光材料中,具有低驅動電壓與高發光效率之優點,故本研究將之選為電洞傳遞層材料。但唯一缺點就是不易溶於溶劑中,所以在實驗驗中有安排質子酸摻雜的步驟,就是為了要改變高分子的電性、磁性、光學性質或結構,同時導電度也隨之增加。本研究以ITO基板改質及無電極電鍍為研究主軸,以此來增加其之性能。改質ITO基板目的,是希望使得ITO陽極表面平坦化且可促使電洞注入,進而增加發光層材料附著力與阻隔電子直接導入陽極之特性,故可增加元件之發光亮度。而使用無電極電鍍法,因為其不須通電就能進行電鍍的反應,行成的薄膜也很均勻。所以採用無電極電鍍法,來完成電鍍的步驟。The polymer light emitting diodes is one of the most important study projects in the field of the conducting polymer due to the low production costs、 simple production process、great surface products that the organic light emitting diodes can not achieved. The purpose of this study is try to utilize the polyaniline as the electric hole transfer layer, and the MEH-PPV as the light emitting layer. The disadvantages of the polyaniline are the low solubility in solvents that was improved by doping proton acids. The performance of the indium-tin oxide was improved by the self-assembling method that made the surface of the ITO became more smooth to help the electric hole injection of electrions. And the aluminum cathode was produced by the electrode less plating.
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