超音波應用於臨床已行之有年，而以穿透式超音波打入腦部的想法也已提出，本實驗目的即為測試低強度間歇性超音波達到腦部受傷後治療的可能。本實驗採用星狀細胞培養模式來探討，首先以腫瘤壞死因子α(Tumor Necrosis Factor-α, TNF-α)模擬中風缺氧的狀態，並利用低強度間歇性超音波刺激星狀細胞，然後檢測其培養液中一氧化氮(nitric oxide, NO)的濃度變化，以及大腦衍生神經營養因子(Brain-derived Neurotrophic Factor, BDNF)、神經膠質衍生神經營養因子(Glial-derived Neurotrophic Factor, GDNF)之mRNA 表現的情形。由實驗結果顯示，在TNF-α存在狀況下則能使NO 濃度上升，施打低強度間歇性超音波可促使NO 增加，並提升上述BDNF、GDNF 兩種蛋白質之mRNA 表現，BDNF 可幫助支持神經元生存，促使新神經元和突觸的發展及分化；GDNF 可有效提昇許多類型神經元生存率；而適當之NO 濃度可以調節神經傳遞物質釋放和腦血流，乃至於影響到記憶的產生。我們初步的結果也顯示在TNF-α存在下，超音波可以增加BDNF 及GDNF 蛋白之產生，因此本研究成果顯示低強度間歇性超音波可提供有利於神經元的環境。The ultrasound technology has been widely applied in the clinical practice for many years, such as monitoring of fetus, lithotripsy, rehabilitation after fracture, as well as the ultrasound stimulation of brain tissue, etc. The purpose of this study is to investigate the therapeutic effect of low intensity pulsed ultrasound (LIPUS) on the injured brain. In this study, we adopted the in vitro model of astrocyte cultures. The tumor necrosis factor- α (TNF-α) was used to simulate the condition of ischemic stroke. We used LIPUS to stimulate the cultured astrocytes, then we ssessed the concentration of nitric oride (NO) in the culture medium. We also assessed the expression of messenger RNA (mRNA) of both brain-derived neurotrophic factor (BDNF) and glial cell-derived neurotrophic factor (GDNF). The results showed that NO increased after the addition of TNF- α. In addition, treatment of LIPUS increased the expression of NO, as well as the mRNA of both BDNF and GDNF. The BDNF is able to support the survival of neuron, as well as stimulate differentiation of neurons and synapses. GDNF can also increase the survival of various kinds of neurons. Furthermore, NO may regulate the secretion of neurotransmitters, increase the blood flow in the brain, as well as enhance the processing of memory. Our preliminary results also demonstrated that both BDNF and GDNF protein increased in response to LIPUS in the presence of TNF-α. Therefore, treatment with LIPUS implicated an environment favoring the protection of neurons.

1. Purpose of research \r To investigate the feasibility of using municipal cellulosic wastes as feedstock for production of biochar in pyrolysis, the effects of metal catalysts in pyrolysis, and the applicability of the produced biochar as a fertilizer\r 2. Procedures \r A. Investigation into the characteristics of (metal catalyzed) pyrolysis of various cellulosic wastes \r 1. The cellulosic waste (and catalyst) was weighed and put into a boiling tube. The tube was stopped with a plastic bung with holes. A plastic tube and a thermocouple were inserted through the holes. The other end of the plastic tube was submerged. \r 2. A Bunsen flame was used to pyrolyse cellulosic waste. Temperature and time of reaction were recorded. Gas produced was collected underwater. Biochar and bio-oil were obtained and weighed. \r B. Evaluation of adsorptive capabilities of different materials \r 1. Blue light absorbances of KH2PO4 solutions (mixed with vanadate-molybdate reagent to form yellow solutions) at different concentrations were found and an absorbance-concentration curve was established. \r 2. 5g of each material being evaluated was sandwiched between two pieces of filter paper before being put into a suction funnel. KH2PO4 solution was poured into the funnels. The setups were left overnight and filtrates were collected. \r 3. Collected filtrates were mixed with vanadate-molybdate reagent. Concentration of phosphates in each filtrate was found by the curve.\r 3. Data \r I. Highest percentage conversion from waste to biochar: 94.1% (paper towel, iron wool) \r II. Highest sequestration rate of carbon: 98.6% (paper towel, zinc) \r III. Lowest pyrolysis temperature: 162°C (paper towel, copper) \r IV. Best catalyst in terms of speed of biochar production: copper (+47.7%) \r V. Highest speeds of biochar production (w/ and w/o catalyst): 46.4g/hr (paper towel, copper) and 27.7g/hr (sawdust) \r VI. Adsorptions of KH2PO4: 14.4% (biochar from sawdust)/ 9.02% (sawdust)\r 4. Conclusions \r The pyrolysis of cellulosic waste to biochar was achievable at school laboratory conditions, with satisfactory results in carbon sequestration, production speed and percentage conversion. \r Under catalysis by various metals, the production of either biochar or pyrolytic gas and oil can be optimized, providing a low-cost way to derive fuel and sequestration-ready carbon, both crucial as answers to looming crises. The use of copper greatly speeds up pyrolysis and lowers the pyrolysis temperature, further increasing the economic potential of the process. \r Biochar is also an effective means to soil management, as shown in field and laboratory experiments. Its adsorption capability far exceeds that of untreated cellulosic waste, retaining nutrients to be taken by plants instead of leaching away. It was also shown to improve fruit yield and induce ripeness in tomato, making it obvious that biochar is also a viable fertilizer. \r All in all, metal-catalyzed biochar production from municipal cellulosic waste and the subsequent use of biochar as fertilizer have the benefits of: low feedstock cost, low energy cost, fast production, carbon sequestration, soil management and waste recycling. It is a remedy to some of the most persistent and serious global problems: food and energy crisis, water pollution, excessive greenhouse effect alongside waste treatment.

PPO 是一種含銅的多酚氧化? (E. C. 1. 14. 18. 1)，主要是將酚類 (phenol) 氧化成二酚類(diphenol)，更近一步的變成quinones，後者是一種不溶於水的褐色聚合物，在植物中造成褐化最主要原因，然而在人類皮膚則產生黑色素 (melanin)。我們提出有一種揮發性的抑制劑存在動植物體內，此種抑制劑會抑制生物體內PPO 的活性。植物在收成後，揮發性的抑制劑逐漸消失，導致內生性的PPO 活性逐漸上升，因此植物便會產生深褐色的斑點。在本研究中，我們以玉蘭花作為例子，以生化酵素動力實驗、部分純化黑色素抑制劑來解釋揮發性抑制劑在植物體內的存在及其作用，更進一步對水果快速褐化提出一種新的理論。研究發現PPO 的褐化反應就像是人體內酪氨酸? (tyrosinase) 的催化反應，酪氨酸?可以使人體產生黑色素而累積在皮膚上形成黑斑，利用〝人工皮膚〞模擬揮發性黑色素抑制物的作用，證明，防止PPO 抑制劑之揮發可能在未來美白保養品工業裡扮演一極具潛力的角色。Polyphenol oxidase (PPO) or tyrosinase (E.C. 1.14.18.1) is an important and ubiquitous enzyme responsible for browning in plants and melanization in animals. PPO is a copper-containing enzyme that catalyzes the chain-oxidation from monophenol or polyphenols to o-diphenols and subsequent o-quinones. The resulting quinones are large wate-insoluble polymers with dark brown color. We proposed that volatile inhibitors are associated with the plant PPO and block the PPO activity in vivo. While post-harvesting the volatile inhibitors evaporate, the endogenous PPO is then activated and therefore instantly produces dark quinone pigment. In the present study using magnolia flowers as an example, we show the presence of a potent volatile inhibitor(s) for PPO in plant. The novel finding clarifies the mechanism involved in the browning phenomenon of post-harvesting for most fruits. Since the PPO is also know present in human as tyrosinase responsible for the formation of “darkening spots” on skin, the finding of evaporation of potent PPO inhibitor may be potentially used as a strategy in developing a novel cosmetic product.

In this study, we try to know how ectopic eye genes: eyeless(ey), eye gone(eyg), twin of eyeless(toy), twin of eye gone(toe) act cooperatively, and look for some unknown genes which affect the function of eyg. First, through human trans gene screening, we find two human genes change the phenotype of ey>eyg into dorsal out-growth when they co-express with eyg (ey>eyg+X). It means the two genes may relate to cell proliferation. Second, by sequencing the insert genes of mutant fly which was found by EP screening, the result shows the site of the insertion is the same as effete (eff) gene. eff translated wrong proteins which differ from functional ubiquitin-conjugating enzyme may be the major cause of the mutant eye . 本研究係探討果蠅複眼異位基因eyeless(ey)、eye gone(eyg)、twin of eyeless(toy)、 twin of eye gone(toe)間的協同作用，並尋找與eyg 有交互作用的基因、突變株。藉由人類基因轉殖篩選，找到兩株人類基因轉殖株，當其與eyg 共同表現時(ey>eyg+X)，會改變ey>eyg 的複眼性狀，造成dorsal out-growth，顯示這兩個基因可能與細胞增生有關，此外，藉由EP screening 複眼發生突變的果蠅之UAS 下游基因經比對後，位置與effete（eff）部分契合，推測複眼發生突變的原因是eff 的功能發生異常，致使細胞內蛋白質代謝失常所致。

南投溪頭米堤飯店在2001 年因土石流受創，產險公司以「土石流即是山崩」為由拒絕理賠。歷經5 年的訴訟後，2007 年1 月台灣高等法院認定，土石流屬保險契約中所規範的「洪水」，並非「山崩」，判決產險公司應給付賠償金。土石流究竟是洪水？是山崩？主要因素便是土石流發生的水文因子。本文藉由博愛村的現場調查及文獻探討先作初步資料分析（preliminary analysis），了解到地文因子(physiographical factor)是土石流發生的充分條件，非必要條件；一般僅考慮雨量因子，把地文因子看作常數（忽略地文因子受水文因子歷程(course)影響），簡單易懂，但在安全與經濟考量上有待討論。使用類神經網路對已發生過的土石流事件計算土石流發生臨界曲線，並使用模糊理論計算松鶴地區其受水文因子影響的土石流發生臨界曲線，這樣的模式，考慮似乎比較周到。由「米堤飯店」的例證，更說明土石流發生的水文因子的必要性。Lemidi hotel, Xitou, Nantou because mudflows and landslides and was wounded in 2001. The Insurance Company refused to settle a claim on the position on “A landside is a debris flow”. After the lawsuit which was going through 5 years. In January, 2007, the High Court of Taiwan asserted that the adversity in the 2001 belongs to “the flood” in the norm of the insurance agreement. As the norm of the insurance agreement said, the debris flows in the land is the flood, not the “landslide”. The High Court of Taiwan judged that the Insurance Company should compensate the Lemidi Hotel. Is debris low a landslide or a flood? The main cause is the Hydrology in the happening of a debris flow. We did preliminary analysis of forms by on-the-spot investigation on Song-Ho Village and reference discussing. We realized that physiographical factor is an abundant condition for a happening of a debris flow, not the essential condition. Generally, people only consider rainfall factor. And they consider physiographical factor as not as constant (neglect the influence of hydrology factor to physiographical factor). We can understand easily in that way. Therefore, in the aspect of security and economy, there is much doubt that is needed to be discussed. People who use neural network method to calculate the curve of the debris flow happening , and used fuzzy theory to calculate the curve of the debris flow happening which is influenced by the hydrology in Song-Ho Village. In that way, we may consider more thoughtful. From the example of the Lemidi Hotel in Nantou, we can prove that how necessary the hydrology factor in the debris flow is.

The purpose of the research was to find the relationship between the fruit fly’s attraction to light (or heliotaxis) and their genes. Fruit flies, because of their short life span of approximately two weeks, and their simple requirements for survival, are ideal candidates for the study. With a specially designed device "Mi Gong" for the experiments, fruit flies could be separated and cultivated in accordance to their preference to specific wavelengths of light. They received cross-breeding and were studied to learn the ratio of the resulting offspring in their preference to the light. It was observed that the majority of the fruit flies were more attracted to violet light. It was also found that the next generation of the fruit fly inherited higher sensitivity to the light than their parents. Knowing that there could be potential margin of errors in the experiments, the results of the study demonstrated that light, as an external source, had limited impact to heliotaxis of fruit fly. The study suggested that insects with heliotaxis, including fruit fly, inherited heliotaxis in their genes. The study also pointed out the potential benefits of employing heliotaxis in many areas especially in the agricultural development. Furthermore, heliotaxis can be utilized to study the difference in the behavior of nightlife insects before and after the invention of manmade light source.這項研究，主要是在探討果蠅的趨光性和遺傳的關係。很多種昆蟲都具備趨光性，我們之所以以果蠅作實驗，主要是因為其生活週期短，大約兩週，且易於培養。實驗內容是以「迷宮器材」來鑑別果蠅對某一特殊色光的偏好，將該群果蠅分離出來培養，令其繁衍後代，觀察其後代對此一色光偏好的比率，我們所用的果蠅大致上對綠色光較敏感，而其子代的敏感度較親代高。因此，我們推論趨光性會遺傳，且受光影響，但並非全由光影響，故這可能是實驗中的誤差成因。雖然此實驗是以果蠅為對象，但亦可假設其它具趨光性的昆蟲，也可能是因遺傳而導致其子代有趨光性，而我們可利用其趨光的一些特性，減少農作物因害蟲類的果蠅而損失，並且希望可以得知,在人造光源還未出現在世上時，夜行性昆蟲是否有差異。

Mathematics and music are two poles of human culture. Listening to music we get into the magic world of sounds. Solving problems we are immersed in strict space of numbers and we do not reflect that the world of sounds and space of numbers have been adjoining with each other for a long time. Interrelation of mathematics and music is one of the vital topics. It hasn’t been completely opened and investigated up to now. This is the point why it draws attention of a lot of scientists and mathematicians to itself. This is the point why it draws attention of a lot of scientists and mathematicians to itself. Having considered the value of these two sciences, it seems to us that they are completely non-comparable. In fact can there be a similarity between mathematics – the queen of all sciences, a symbol of wisdom and music – the most abstract kind of art? But if you peer deeply into it you can notice that the worlds of sounds and space of numbers have been adjoining with each other for a long time. In the work I will try to establish the connection between mathematics and music and to find their common elements, to analyze pieces of music with the help of laws and concepts of mathematics to find a secret of mastery of musicians using mathematics and also to investigate the connection of music with mathematics with the “research part”. They are my own calculations and researches which are an integral part of the work. The connection of mathematic and music is caused both historically and internally in spite of the fact that mathematics is the most abstract of sciences and music is the most abstract kind of art. V. Shafutinskiy, I. Matvienko, m. Fadeev, K. Miladze, Dominik the Joker – modern composers of the XXI century – have used the golden proportion only in 4% of their pieces of music and more often in romances or children’s songs. I have revealed this fact after investigating their pieces of music of different genres. However there is a question: why does modern music attracts all of us more but the classics is being forgotten? Investigating connection between mathematics and music I had come to the conclusion that the more deeply the piece of music gives in to the mathematical analysis, to research and submits to any mathematical laws, the more harmonious and fine its sounding is, the more it excites human soul. Besides I am convinced that many important, interesting and entertaining things have not been opened in this field. We can safely continue our research of these things. I think that I have managed to lift a veil over mathematics in music, to find something common for apparently incompatible science and art. In due time English mathematician D. Silvestre called music as mathematics of feelings, and mathematics – as music of intellect. He expressed hope that each of them should receive the end from the part of the other one. In the future he expected the occurrence of a person in which Beethoven and Gauss’ greatness would unite. Terms ‘science’ and ‘art’ practically didn’t differ during far times of antiquity. And though roads of mathematics and music have gone away since then music is penetrated with mathematics and mathematics is full of poetry and music!

本實驗的觀測乃著重於觀測各定點之臭氧濃度與該地天氣因子；如溫度、相對溼度、氣壓、雲量、風速、日照強度等與之比較並控制所有可能的變因，來推測一地空氣污染的程度，並從中思考影響一地臭氧濃度變化的要素。 利用自製的熊本試紙來測量在對流層中臭氧的濃度，進而來推論出我們所設的測站附近的空氣污染程度。 由實驗了解臭氧濃度和其他天氣因子如溫度、相對溼度、風向、風速、日照強度、紫外線強度、工廠作息或交通流量等因素有著很微妙的關係。 最後，我們歸納出在做此實驗時所遇到的相關問題與解決方法。 This experimentation is about the ozone of troposphere. We try to find out how the weather elements affect the ozone consistency (for example: air temperature, relative humidity, air pressure, cloudage, wind speed, solar insolation), and to discover the relation between the ozone consistency and the air pollution. We use the test paper which is made by ourselves to measure the ozone consistency of troposphere, so that we con use the date to infer the air pollution level at the area where we conduct our tests. According to our experiment, we find out the ozone consistency and other weather elements (ex: air temperature, relative humidity, air pressure, cloudage, wind speed, solar insolation or traffic), have some delicate relations with each other. Finally, we conclude all the relative problems we face in this experiment and their solutions.

My school requires year 13 students to complete a year long project of a topic of their choice, culminating in the presentation of a thesis, a display and speech to a public audience. Many different topics appealed, but in the end I decided to build a micro wind-turbine. I have always been fascinated with mechanics, mathematics, engineering, aerodynamics and electricity. A wind turbine is a mixture of these technologies, with the overall goal of electricity production. In a world that is starting to see the true costs of fossil fuels, renewable energy seems to be increasingly popular and the demand for electricity is always growing.\r I was aware that building a wind-turbine from the foundations up wouldn’t be easy. Many of the experts I contacted in the early days cautioned me against trying such a complex thing in one year, at the same time as completing a full Year 13 course. There were, however, people prepared to support me. Michael Lawley, who builds micro wind turbines in New Plymouth was very helpful, just full of priceless knowledge and gave me a few basic parts to start with. The knowledge gained from Doug Clark, who also builds his own 11 kW wind turbines, was such an inspiration. Later I had practical help from Wilson Springford and Darron Matthews.\r I investigated and documented the history of and current state of wind technology, as well as my own experience and learning in the design, construction and testing process. I thought it would be interesting to find out how the electrical and mechanical side works.\r The generator, a washing machine motor, needed to be completely rewired, and converted to DC (direct current) from AC (alternating current). I built my own 3-phase AC to DC converter.\r Other parts like the disc brakes and bearings had to be found. The rest was hand-made and every part, to a certain extent, had to be modified. Probably more than twenty braking system design attempts led to the final decision to incorporate the wind-activated hydraulic disc brake where the wind paddle starts to ease the brake on over a certain wind speed.\r I studied the dynamics of wind turbine blades, their shape, the material they were made from and how this affected their performance. The decision to make my own blades helped me gain a great sense of achievement and knowledge of blade design. I found some New Zealand made 100% recycled plastic pipe, an added bonus because I wanted to have minimal environmental impact. I designed the turbine with three blades to give better starting torque along with a lower top speed, perfect for how I wired the generator.\r I designed the swivel, the part of the wind turbine that enables the power cables to get from the turbine down the tower without twisting up and has the job of carrying the whole turbine, which is mostly made from recycled aluminium. The steel and bearings used to create the swivel were all second-hand parts and materials. The power from the turbine passes through the swivel into the cables and down the tower. The main mast of the tower is a little over 4.7 metres and pivots on two shorter supporting poles which go down around 2.6 metres to the bottom of the reinforced concrete foundation.\r I managed to, design and construct an operational prototype micro wind-turbine, incorporating recycled and recyclable materials as much as possible, with the end result surpassing all expectations.

中文摘要 本實驗先尋求將廢棄保麗龍磺酸化為陽離子交換樹脂(本實驗稱”保麗龍膠”)的方法。將保 麗龍依：丙酮溶解→硬化→打碎→與濃硫酸共煮三小時→浸於50%硫酸溶液中→沖洗→以水 浸泡的流程，即可達再造的目的；我們測得其磺酸化比例為62.5%。再利用「碘滴定法」(浸 泡式)與「相對電壓檢測法」(流動式)，依次尋求保麗龍膠吸附金屬離子的最佳條件。其中「碘 滴定法」可有效測出銅離子濃度，但手續繁瑣；「相對電壓檢測法」最大的好處是知道保麗龍 膠何時吸附達飽和必須再生。 目前我們所知，要保麗龍膠達到吸附陽離子的最佳效能，其條件依次為：使用細粒的保 麗龍膠；低濃度的金屬離子溶液；質量愈大的保麗龍膠；低溫下較慢的金屬廢水流速及pH 值約為4.30 的銅離子廢水；鈉型的保麗龍膠吸附效能優於氫型。保麗龍膠對不同金屬離子亦 有吸附力，單位體積所含離子數愈少，初始的相對電壓會愈高；在相同莫耳濃度下，不同離 子的吸附力依次為Cr3＋＞Fe3＋＞Ni2＋＞Cu2＋＞Co2＋；分次吸附確可將金屬離子完全去除；由 吸附等溫線觀察得知，可能保麗龍膠為多孔物質，導致500ppm 以下的吸附模式無法明確判 斷，1000ppm 以上則為物理吸附模式；保麗龍膠可以再生也可被覆在砂粒上達到不錯的吸附 效能；最後，我們將吸附過金屬離子的保麗龍廢膠與硫酸鈣、紙漿及些許的石灰(質量依序為 13 克、13 克、7 克、0.04 克)混合，可製成類似紙黏土，做成造型磁鐵，廢物利用十分有趣。 Abstract The Experiment will, first of all, explore the ways to sulfonate expandable polystyrene into cation ion exchange resin (called “polystyrene rubber” hereafter in the experiment). The procedures of treating expandable polystyrene are as follows: acetone dissolve→hardening→smashing→ boiling with sulfuric acid for three hours→immersing in 50% sulfuric acid solution→washing→ immersing in water so that we may reach the goal of reconstruction. We calculate the sulfonated rate to be 62.5%. Then we make use of “Iodine Titration”(immersion method) and “Opposite Voltage”(floating method) to seek for the best conditions of adsorption the metallic ion through polystyrene rubber. The former can effectively calculate the concentration of copper ion, but the procedures are quite complex. The greatest advantage of the “Opposite Voltage” method is that we may know when the adsorption of polystyrene rubber is saturated and should be regenerated. As far as we know at present, the conditions of obtaining the best effect that polystyrene may adsorb the cation ion are as follows: fine particles of polystyrene rubber; low concentration metallic solution; polystyrene rubber of which the mass is greater; at lower temperature, slower waste water flow speed and the copper ion waste water with pH 4.30; the adsorption effect of sodium type polystyrene rubber is better than the hydrogen type. Polystyrene rubber also has adsorption effect toward different metallic ion. The less ion per cubic contains, the higher the original opposite voltage. With the same mole concentration, different ion adsorption effects may range as follows: Cr3＋＞Fe3＋＞Ni2＋＞Cu2＋＞Co2＋. The batch adsorption definitely may erase metallic ion completely. By observing the adsorption isotherm, possibly because the polystyrene rubber is a multi-apertured matter, we find that it is impossible to judge exactly the adsorption model of those metallic ion solutions of which the concentrations are below 500ppm. Those which are over 1,000ppm belong to physical adsorption models. Polystyrene may be regenerated and get an adsorption effect by coating sand particals. In the last analysis, we may make paper clay and magnets of different styles by mixing the adsorbed metallic ion polystyrene rubber with calcium sulfate, paper pulp and a little lime(the mass are respectively 13g, 13g, 7g, and 0.04g). The reuse of waste is really very interesting.

1.Purpose of the research We observed frequent application of chemical pesticides and herbicides at the pear orchards near our school. Our concerning questions are the influences of agricultural chemicals on soil ecosystem which may be slowly deteriorating. There are many other orchards where the farmers are practicing environmentally-friendly agriculture using organic fertilizers such as composed manure instead of sycnthetic fertilizers. The purpose of this research is to examine chemical and mocrobiological quality of the orchard soil. We report biological control of pear disease using antagonistic microorganisms and suggest improvement of soil quality as a result of nature friendly agricultural practice. 2.Procedures and Data Two orchards were selected: Conventional fertilizers and chemical pesticide application farm; Environmentally friendly farm using organic plant manure and biological control of disease. Several different aspects of soil micro-flora, indicator microorganisms, physiological characteristics of pear fruit, residues of synthetic chemicals in the soil, soil chemical properties were examined. We could observe that use of organic manure and biological control agents for disease control by indigenous microorganisms improved biological and chemical quality of the orchard soil. Results are summarized as follow: A. Microbial diversity could be observed in organic farming orchard soil. Among 42 fungal genus, 7 species of penicillium including P. decumns, 3 Alternaria species, 4 Fusarium species including Fusarium graminearum, 6 Aspergillus species including Aspergillus niger were identified. In case of bacteria, 59 samples were isolated and 17 genus including Cystophaga johsonae were identified. B. Beneficial indigenous bacteria having several biological functions were isolated: Enterobacter intermedium produces organic acid therefore this bacterium can solubilize insoluble phosphate. Several Pseudomonas species were isolated. These bacteria produce iron chelating compound under iron limited environments and can control plant disease. Interestingly, we could find indigenous biocontrol agent Hypocrea vinosa which can effectively control black star disease on pear surface. C. We have formulated ten different microbial-pesticides using several combinations of indigeous microorganisms such as Hypocrea vinosa, Trichoderma atroviride, Rhizopus microsporus etc. These microbial-pesticides could inhibit 100% of black star disease on pear surface which showed similar inhibition effect as commercial pesticides indicate that these microbial-pesticides may replace synthetic chemicals in the near furture. 3.Conclusions Our research demonstrates the eco-friendly organic farming system could improve soil eco-system of pear orchard, where no synthetic fertilizers and pesticides are applied. As a result of manure application, the soil chemical properties are more stable and microbiological flora was more diverse. Use of microbial and bio-pesticide resulted in strong inhibition of black-star disease incidence on pear surface. We suggest that eco-friendly farming systems are needed to diminish environmental pollution in soil and water caused by frequent and abundant use of agricultural chemicals.

台灣溫泉資源豐富，不同的溫泉泉質適合不同的溫泉藻類生長。溫泉藻屬於極端生物，其應用資源極待研究開發。本研究根據細胞型態、藻膽蛋白種類分析以及rbcL 基因定序等結果推測北投溫泉藻屬於一種溫泉紅藻，其種類近似於Galdieria sp. (Rhodophyta)。此外，本研究利用葉綠素螢光分析儀於野外實測北投溫泉紅藻於不同光強度下，光合作用能力的差異，發現適應於不同光強度的溫泉紅藻，其電子傳遞速率並無顯著的差異。推測其原因可能與北投溫泉紅藻的phycocyanin 含量會隨著光量有所調整，藉此達到最佳的光合作用能力有關。另外，本研究發現隨著光強度的上升，溫泉藻的NPQ 值有上升的趨勢，然而低光區的溫泉藻有較高的NPQ 值，此與一般理論不符。利用HPLC 分析北投溫泉紅藻的類胡蘿蔔素種類組成，其種類分別為Lutein、α-carotein、β-carotein 與Zeaxanthin，未發現Violanxanthin 與Antheraxanthin，北投溫泉紅藻並不俱有葉黃素迴圈。因此根據研究結果，我們認為NPQ 值與色素的關係仍有討論的空間Taiwan is resourceful in hot springs. Various hot spring algae are adapted to different types of hot springs. Hot spring algae applications are still under developing. In this work, based upon the morphology, analysis of phycobiliproteins, and rbcL sequences, the hot spring algae of Peitou is found to be Galdieria maxima (Rhodophyta). In addition, the dependence of the photosynthesis of Galdieria maxima on the light intensity was measured by Diving-PAM. The results show that the light energy availability efficiency of Galdieria maxima adapting to different light intensity exhibited different, although the electron circulate rate differed insignificantly. This suggests that the concentration of phycocyanin in Galdieria maxima may vary to attain optimal photosynthesis. Furthermore, the NPQ of Galdieria maxima increase with the light intensity. However, contradict to the theory; even under the same light intensity, the NPQ of Galdieria maxima was higher at low light zone. The carotene composition of Galdieria maxima was analyzed using HPLC and found lutein, α-carotene, β-carotene, and zeaxanthin. Violaxanthin and antheraxanthin were not present. Therefore, Galdieria maxima do not exhibit xanthophyll. Based on the results of this study, the correlation between NPQ and pigment still needs to be investigated.