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In silico identification and physicochemical analysis of potential novel antimicrobial peptides from Momordica charantia L.
The emergence of antibacterial resistance has necessitated the development of alternative treatments, such as antimicrobial peptides (AMPs). AMPs are part of the innate immune systems of various organisms such as Momordica charantia L., a known medicinal plant in Southeast Asia. In this study, potential novel AMPs from M. charantia were derived in silico to provide prospective antibiotic alternatives using promising plant-based peptides. M. charantia protein sequences that were 500 amino acids long were digested using proteolytic enzymes, resulting in 3,621 peptides. Each resulting sequence was characterized as either AMP or Non-AMP using four statistical analysis tools, and those identified as AMPs were analyzed. This led to 102 AMPs, 53 of which were unregistered on the Data Repository for Antimicrobial Peptides, indicating that they have yet to be derived from other species. Six of the eight studied physicochemical properties show strong correlations with each other, suggesting that subsequent AMP design studies may focus on these six properties. As such, M. charantia may be a rich source of potential AMPs and, thereby, alternative antibiotics. The in vitro examination of these novel AMPs is also recommended to further understand their potential as alternative antibiotics sourced from locally available plants.
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Study of regenerative and ontogenetic processes under the influence of EHF EMR.
The increased sensitivity of aquatic organisms to the effects of EMF has been proven by numerous experimental studies. It has been repeatedly noted that exposure to EMF of certain frequencies and intensities leads to disruption of physiological functions, orientation in time and space, changes in the behavior of organisms, suppression of motor activity. Other ranges of electromagnetic radiation, on the contrary, can cause the effects of increased regeneration, growth rate and survival. In connection with these trends, the purpose of our research is to analyze the effects of the influence of electromagnetic radiation of extremely high frequency on the development of the Xenopus laevis and the regeneration of newts and planarians
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由誘導性多功能幹細胞篩選新生抗原用於大腸直腸癌之抗癌應用
癌症治癒方式成效有限,在iPSC中找出具有潛力的蛋白製成大腸直腸癌疫苗是本研究目的。 實驗先以C57BL/6小鼠之股骨及脛骨骨髓中造血幹細胞分化成為未活化的樹突細胞。放入安慰劑、CpG佐劑、CpG+MC38、CpG+iPSC等抗原物來進行免疫細胞活化測試,流式細胞儀分析結果驗證能否減緩小鼠大腸直腸癌的腫瘤生成。接著,利用質譜儀及大數據分析從iPSC中找出促使樹突細胞活化的抗原物蛋白成分,選出3個候選,透過西方墨點法確認其專一性。查詢候選蛋白TTW1、2、3在The Human Protein Atlas 網站對大腸直腸癌之表現數據。 結果 iPSC的抗原物促使樹突細胞的MHC1標記物活化,助於毒殺性 CD8+T細胞的產生進而透過免疫系統預防大腸直腸癌的產生。TTW1只有在iPSC細胞株表現出專一性,同時資料庫顯示TTW1在大腸直腸癌中具有高表現量。 目前未有文獻探討抗原疫苗的應用,團隊未來專注合成iPSC中TTW1蛋白,進一步開發成大腸直腸癌疫苗。
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surgical masks and microplastics in our airways
The surgical mask has been our daily companion since the outbreak of the Corona pandemic. The nonwovens (outer layers, not the filter membrane) from which the surgical mask is constructed consist of very long and thin polypropylene fibers. This leads to the question of whether microplastics are released during breathing through the surgical mask, which could enter the respiratory tract or the lungs. This would have a negative impact on our health, depending on the size of the detached fiber fragments - the smaller the worse because they can enter much deeper in our respiratory tract. In order to investigate the question of whether fiber fragments are released during breathing through a surgical mask, a filtration device was built. The filters were examined under an optical microscope after filtration. If fiber fragments would detach from the surgical mask, they would be found on the filter. Different surgical masks were tested, those that were not worn at all to surgical masks that were worn all day. It was found that fiber fragments were coming off the surgical masks. There were different fiber fragment types. Some fiber fragments were still undamaged (exhibited nice fractures), while others were frayed. Clump-like fragments occurred, but also smaller fine fiber fragments. All these different fiber fragments had a certain size, so that they could be called microplastics. The remarkable result of the whole study is that there is a direct correlation between the wearing time of the surgical mask and the number of detaching fiber fragments. In the case of the unworn surgical masks, 10 times fewer fiber fragments occurred during filtration than in the case of the surgical masks that were worn all day.
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Utilization of Escherichia coli in Contiminated Water in the Citarum River as a Dual Chamber Baed On Microbial Fuel Cell (MFC) Substrat
Citarum River is the longest and largest river in West Java. The upstream of the Citarum River starts from Mount Wayang, Bandung Regency and ends at the mouth of the Java Sea which is located in Muara Gembong, Bekasi Regency. The Citarum River plays an important role as raw water for PDAM drinking water, supplies electricity in Java-Bali and provides water for rice field irrigation in West Java. Citarum watershed is dominated by the manufacturing industry sector such as chemicals, textiles, leather, paper, pharmaceuticals, metals, food and beverage products, and others. Based on data from the World Bank, every day, the Citarum River is polluted by approximately 20,000 tons of waste and 340,000 tons of waste water with the majority of the waste contributors coming from 2,000 textile industries. By looking at these events, there is no doubt that the sustainability of the ecosystem and the environment in the Citarum River is damaged and polluted. (Zahra Fani Robyanti; 2020). The West Java Regional Environmental Management Agency stated that the content of E. coli bacteria in the Citarum River had increased. The bacteria that cause diarrhea come from industrial and household waste. In addition to E. coli bacteria, other pollutants in the Citarum River that have increased are biological oxygen demand (BOD), chemical oxygen demand (COD) and Suspended Solids. One of the efforts that can be done regarding E. coli bacteria that pollute the Citarum river is to make it as a substrate for Microbial Fuel Cell (MFC). Bacteria present in organic media convert organic matter into electrical energy. The nature of bacteria that can degrade organic media (enrichment media) in MFC produces electron and proton ions. It is these ions that produce an electric potential difference so that energy can be generated. Generally in conventional systems, MFC consists of two chambers consisting of anode and cathode chambers. The two spaces are separated by a membrane where proton exchange occurs. This system has not fully worked with bacteria because only the anode side contains bacteria, while on the cathode side it still works using chemical compounds such as Polyaluminum Chloride (PAC). However, recently MFC has been developed using bacteria at the cathode, or better known as biocathode. Bacteria in the cathode space have the same function as electron mediators that were previously carried out by chemical compounds. In many studies on MFCs, acetate is commonly used as a substrate for bacteria to generate electricity. These chemical compounds are easier for bacteria to process than wastewater. Acetate is a simple chemical compound that serves as a carbon source for bacteria. Another advantage of acetate is that this compound does not cause other reactions to bacteria such as fermentation and methanogenesis at room temperature. Based on this thought, the author will design a study entitled Utilization of Escherichia coli Bacteria in Contaminated Water in the Citarum River as a Dual Chamber Based Microbial Fuel Cell (MFC) Substrate.
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Silver Moringa Cloth: Silver Nanoparticle Fabric Based on Moringa Extract (Moringa oleifera) as Antibacterial Against Methicilin Resistant Staphylococcus aureus
Staphylococcus aureus is addressed as one of the most common pathogens in hospital settings and in the community. This pathogen causes invasive infections, sepsis, and death. The emergence of antibiotic-resistant bacteria is due to bacterial mutations and the use of antibiotic drugs that are not by procedures. Resistance makes MRSA infections difficult to treat, resulting in high healthcare costs. These problems lead to an urgent need to find alternative drugs to control MRSA infection. Therefore, developing new drugs and procedures such as antibacterial nanoparticles, are particularly promising. Indonesia has many medicinal plants with antibiotic activity, including Moringa oleifera. Moringa oleifera contains several active compounds such as alkaloids, flavonoids, and saponins which are known to have antibiotic activity. Silver nanoparticles or AgNPs are currently used as antimicrobial agents because they are toxic to prokaryotic cells (bacteria) but relatively safe for eukaryotic cells. AgNP synthesis mediated by M. oleifera extract has the advantages of being non-toxic, pollution-free, and environmentally friendly. Sisal is a potential source of naturally derived fabric and a prospective source of multifunctional textiles. Recent studies have utilized and functionalized sisal to develop composite materials. However, functionalizing of sisal using nanosilver-based materials has not been studied yet. Bioactive chemicals from plant-extracted nanoparticles also provide additional antimicrobial properties. This study aims to produce AgNPs mediated by M. oleifera leaf extract and to analyze its antimicrobial effect on MRSA growth. The powdered Moringa (4g) was boiled with 100 ml of distilled water (550 C) for 15 minutes. The mixture was filtered through Whatman No 1 filter paper and store refrigerated. The nanoparticle was synthesized by rinsing sisal fabric cloth to several concentrations of AgNO3 (1mM, 10mM, and 20mM) with Moringa extract. Nanoparticle synthesis from AgNO3 done with the help of Moringa oleifera extract. The resulting AgNPs have MIC values (Minimum Inhibitory Concentration) and MBC (Minimum Bacteriocidal Concentration) of 1.25 mg/ml. The resulting silver nanoparticles showed antibiotic activity against MRSA with an average inhibition zone diameter of 15.677 mm. XRD and SEM studies are going to be held to support the data.
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Overcoming Motion Disorders
Helping in overcoming the motion disorders is an important thing which deserve striving for. Every year number of people who have MS or paresis (paraparesis) is increasing. The device has effective results for the patients and it backs to finding the conditions of natural treatments in the device. Obviously, this device helps them to keep standing and achieve the required exercises the movement like normal people by using their hands. The degree of improvement differs from person to another as depends on the hardness of the disease and injury kind.
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DECREASING CANSAT ANGULAR VELOCITY USING DEPLOYABLE FINS
CanSat (a can-size satellite) flight data revealed the occurrence of high spin angular velocities along the vertical axis of a CanSat during a parachute descent phase. A novel aerodynamic stabilization system of deployable fins was designed to decrease angular velocity. Deployable fins were attached to servomotors (rotary actuators) to provide control authority during the CanSat descent phase. Deployable fins positions were calculated based on an onboard gyroscope data using a PID (proportional-integral-derivative controller) regulator and a moving-average filter. After the assembly and the initial testing, the system was flight-proven by dropping it from a drone with and without enabling the stabilization system
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本作啟發自線上的影片「Can you solve the giant iron riddle?」,內容是在有外觀無法辨認的4顆有電與4個沒電的電池中,在一次只能取2顆電池測試的限制下,以最少的次數找到2顆有電電池的方法,結論是以分組的方式進行測試會有較佳的結果。本作先驗證影片的方法有效後再進行推廣,得到找出2顆有電電池較佳的方法為分組法或直接取法。再嘗試推廣至取3顆電池的情形,發現除了分組法與直接取法外,還可使用「X2Y1循環檢查法」。最後改良「X2Y1循環檢查法」後,不但可以有系統地測試所有可能的情形以外,能更有效地減少測試次數,提高尋找有電電池組的效率。
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全等三角形與四邊形構造的衍伸圖形之性質研究
本研究源於 2016 年數學雜誌 Crux Mathematicorum 的三角形定性問題[1],我們將這個問題由四個方向進行推廣——多邊形邊數、頂點連線方式、等腰三角形角度、全等多邊形的夾角,再創新探討其定量與定性性質。首先,我們將任意全等的三角形與任意四邊形夾角為任意實數下的衍伸圖形之定量與定性性質進行完整刻劃,利用測量師公式分別針對不同連線情形下的兩個衍伸圖形的有向面積之和與有向面積之差進行完整討論,再巧妙利用平移不變性處理行列式級數和而給出面積不變量關係式。第二,透過向量、矩陣運算與純幾何方式探討面積不變量的幾何意義,並給出衍伸圖形之間的全等、相似、透視、對稱、共線、平行、退化等優美的定性性質。最後,我們系統性推廣到平面上任意封閉多邊形,嘗試以較高的觀點切入,透過矩陣變換給出其幾何結構,並且發現許多實質有趣的定性與定量統一結果。
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Carbone monoxide filtre
Carbon Monoxide is a very toxic and dangerous that threatens our life and can cause sudden illness and death. It is the most abundant, by mass, pollutant gas generated by the engine due to the lack of oxygen and thus presented in our lives. It is true that the oxidation catalyst absorbs carbon monoxide from the exhaust of cars during combustion. But that is not enough, the catalyst is only effective when the exhaust temperature is high (more than 400°C) which is not available in a short path. To protect ourselves from this toxic gas, we must find solutions and innovative ideas to fulfill this objective. And this is how our project was created. Our focus in this project is to create a filter that can absorb carbon monoxide using the minimum of energy possible. It will be more efficient unlike the traditional method that not only needs high range of temperature (higher than 400°C) but also takes a long period for the reaction to occur.
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PBC~ A home-use detection device for brain tumors that compress the brain stem and optic nerve
腦部腫瘤是沉默殺手, 藏匿在腦中數十年。 一旦發作,通常都會造成巨大的影響。雖有數種高階儀器,可以檢測。但檢測過程繁複、 等待時間以及價格, 對於民眾都是不小的負擔。 腦部腫瘤的診斷方式為: 神經內、 外醫生會用筆燈對患者做初步的瞳孔光反應檢查。 藉由患者瞳孔縮小的速率,判斷是否要安排進階的檢查。但此行為仰賴醫生的經驗,沒有統一的方法及數據可供判斷。 本研究設計一款成本約$1,300 元, 重量僅 159 g,圓柱直徑與柱高皆約為 7cm 的隨身裝置。 藉由 MCU 控制攝影機, 頂部 1.44 吋 TFT 螢幕可即時顯示患者眼部狀態,檢查結果計算後, 也會立即顯示在螢幕上。除提供醫護人員即時數據化的解讀患者狀態。更協助醫護人員在做瞳孔光刺激檢查時,有科學化的標準。
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