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氧化壓力影響基因轉換表現對脂肪分化之作用
細胞脂肪分化是造成肥胖、骨質疏鬆、和糖尿病的重要前置因素。我們若要維持良好身材又想省去減肥藥的問題,那麼我們必須了解造成脂肪化的原因,才可能擁有好的預防之道。利用人類骨髓間質細胞可以分化成骨質與脂肪等細胞的特性,我們研究氧化壓力對間質細胞\r Ras基因轉換表現後骨質與脂肪分化的影響。結果發現以添加超氧根 (O2-,l5nM)形成氧化壓力,可促進正常Ras基因表現的間質細胞朝骨質分化;相反地,超氧根會促進\r Ras基因突變而不表現的細胞,朝向脂肪分化的現象。進一步研究其作可原理,發現氧化壓力可促進 RaS蛋白質啟動細胞外訊息活化酵素(ERK),接著驅動骨質轉錄因子(CBFA1)表現,再到骨鈣蛋白質與骨結節形成。而抗氧化酵素(超氧根轉化酵素;SOD,5OOU/ml)的作用,可以抑制正常Ras基因細胞氧化壓力下骨質分化的進行;但不能防止氧化壓力促進Ras基因突變細胞,朝向脂肪分化的作用。總結而言:Ras基因的表現與否,是決定脂肪分化的關鍵切換點;也是影響氧化壓力對間質細胞朝骨質分化的樞紐。這種基因與氧化壓力互動影響骨質與脂肪分化的剖析,將有助於提醒人們:使用抗氧化劑來調節抗衰老、肥胖、和美容時,必須是在不同情況和不同基因體質的人,有所不同。
\r Human\r mesenchymal stem cells are able to differentiate into bone, muscle, cartilage or\r fat tissues. Our preliminary study with human mesenchymal cell line (HS-5) showed\r that HS-5 cells could differentiate to bone, cartilage and muscle but not fat cells\r as determined by histochemical staining of phenotypes. We have further studied the\r influence of oxidative stress on the switch between bone and fat cell differentiation.\r Results showed that oxidative stress started with exogenous superoxide, produced\r by the interaction of xanthine oxidase and hypoxanthine, promoted the differentiation\r of osteogenic lineage showing expression of osteocalcin and bone nodule formations.\r The mechanism was investigated and superoxide was found to induce ERK (extracellular\r regulated signal kinase) activation; and then the expression of osteogenic specific\r transcriptional factor (CBFA1). A plasmid containing ras-mutant (Ser 17 Asn) which\r can inactivate the expression of ERK was transfected into the HS-5 cells for studying\r the influence of oxidative stress on ras-mutated mesenchymal cells. Surprisingly,\r it was found that oxidative stress did not promote osteogenesis but it enhanced\r adipogenesis from the ras-mutated HS-5 cells. Further studies indicated that superoxide\r neither induced ERK activation nor CBFA1 expression, but it did enhance expression\r of adipogenic specific transcriptional factor (C/EBPα) and lipoprotein lipase in\r the ras-mutated mesenchvmal cells. Taken together, the study model to induce the\r bone cell differentiation from human mesenchymal stem cells may be employed to make\r bone cells for tissue engineering.
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Fenorhythmes Of Yakut Taiga. The Calendar Of The Nature Of Olekminsk Reserve.
“Natural phenological per iodization of a year is called nature calendar…”\r The role of rivers in nature and man’s life is great. They connect people and cultures, form climate, give life to the thousands of living beings. \r In the tasks of researches there were:\r 1. To determine the peculiarities of seasonal dynamics of alive and inanimate nature of Olekminsk reserve;\r 2. To find out (to reveal) the peculiarities of phenological seasons of the territory of Olekminsk reserve. \r _ To determine of phenoindicators which are separate from phenological seasons \r _ To establish the middle dates of the advance of phenological phenomena \r _ To determine the duration of phenological seasons of a year.\r According to the general nature of plants the territory Olekma-Amga interriver refers to the provincial of pine-needles taiga zone, to Verkhne-Lensky flora area. Predominating part of the plants of the reserve relates to boreal types. The flora of the superior plants of Olekminsk reserve includes 654 types. The fauna of area is presented by 40 types of mammals from 45 living in the south of Yakutia, 187 types of birds, 2 types of amphibians and 2 types of reptiles.\r Besides, phenological researches were conducted on the testing area of school ecological control and during arranging of field ecological schools since 2000 till 2010. \r In all 169 phenological phenomena were analyzed from different phenological seasons of year for the last seven years. The gross amount of analyzed information composed 4000 information units.\r Results and conclusions:\r 1. The peculiarities of the seasonal changes of natural complexes were determined for the first time for south of Yakutia on the example of the territory of Olekminsk reserve. The calendar of the nature of Olekminsk reserve was composed. The analysis of phenological observations allowed establishing the row of phenoindicators, with the help of which we can determine advance of that or either phenological stage, as well as the disposition of current vegetative period can be forecasted. The information maybe used in organizing of the measures of nature protection, struggle with pests and the diseases of useful plants, parasite and tranmissive diseases of man and home cattle.\r 2. Geographic position and climatic peculiarities of territory form the peculiarity of seasonal rhythms of Olekminsk’ reserve’s nature.\r 3. All components of landscape in its seasonal changes tightly are connected with each other with causative-investigation ties having formed the definite complex of characteristic phenomena per the stage of seasonal development. \r 4. The carried out analysis of the primary materials of the Annals of the nature of the reserve and information from the field of ecological schools for seven years since 2001 till 2007 allowed detecting the peculiarities of phenoclimatic seasons of the territory of Olekminsk reserve.\r 5. Phenological and temperature outsets of phenological stages were determined.\r 6. Intervals and average of many years dates which are characteristic for phenological phenomena are determined.\r 7. The calendar of the nature of Olekminsk reserve was made on the basis of the processed information.
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Gene Targeting Using Chimeric RNA-DNA Oligonucleotide for Capase-10 in Various Cell Types
The process of gene targeting via Chimeraplasty is achieved by using a RNA-DNA oligonucleotide homologous to a gene of interest ,to introduce a single base pair or frameshift mutation in genomic DNA .The extensive use of chimeraplasty is currently limited by wide variation in its gene conversion rates(.01-40%) and its mechanism of action remains to be fully understood. For cell studies, chimeraplasty is an alternative strategy to homeologus recombination in generating gene knockout models.
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聚苯胺導電高分子在鐵系金屬防蝕上的應用與研究
聚苯胺導電高分子的發展蓬勃迅速,其應用也愈趨廣泛,金屬防蝕為其可能應用之一。台灣四面環海,工業發達,造成金屬腐蝕損害嚴重。本文為探討聚苯胺導電高分子在鐵系金屬防蝕上之應用,針對以下各點進行研究: ( 1)探討導電高分子聚苯胺性質與合成方法(2)探討聚苯胺導電原理(3)測試不同腐蝕條件下聚苯胺的防蝕效果(4)探討聚苯胺防蝕原理(5)各種氧化還原態聚苯胺防蝕效果之比較。實驗結果顯示塗布聚苯胺與未塗布聚苯胺之鐵系金屬(生鐵、不鏽鋼、鍍鋅鐵)在 3 %氯化鈉、 0 . 5M 鹽酸、1M 鹽酸溶液中之腐蝕狀況,以鹼式中問氧化態聚苯胺( Emeraldine base 。)最具防蝕效果。聚苯胺防蝕機構包含阻隔防護、腐蝕抑制劑、陽極保護、惰性化、形成保護性氧化物薄膜、阻礙離子擴散速率、電化學介面遷移等機構 ·The development and application of the conducting polymer polyaniline is getting prosperous and popular. One of the applications is corrosion protection. Because Taiwan is surrounded by sea and the pollution of industry is more and more serious than before the damage of corrosion is greater and should be properly controlled. In this paper, the authors attempted to study the effect of the corrosion protection of polyaniline in ferrous metal. This study could be divided into four parts: (1) the study of the property and synthesis methods for the conducting polymer polyaniline, (2) the conduction theory of the polymer polyaniline, (3) The corrosion protection effects of the polymer polyaniline under different corrosion situations, (4) the corrosion protection theory of the polymer polyaniline, and (5) the comparison of the corrosion protection effects of various polymer polyanilines. According to the experimental results the Erneraldine-based polyaniline gets the best corrosion protection effect. The mechanism of corrosion protection may be the result of the following ones: barrier protection, corrosion inhibitor, anode protection, innobeling, chemical active layer. inhibition of diffusion rate, and the shift of electrochemical interface.
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Molecular and Cellular Responses under Hypoxic Stress among Rice Cultivars with Different Flooding T
全球暖化造成水災頻繁,嚴重威脅植物生存。看似耐淹水的水稻,在完全淹水下亦有其生存危機。水稻FR13A 因耐水性極佳而常用於分子育種,IR64 產量高卻不耐水。是那些特質使稻種間有不同耐水機制?我們觀察其幼苗淹水24 小時後生長情形、通氣組織 (aerenchyma) 變化及應用即時反轉錄聚合?鏈反應 (real time RT-PCR),研究酒精醱酵的主要蛋白質:乙醇脫氫? (alcohol dehydrogenase, ADH1, 2) 及丙酮酸脫羧? (pyruvate decarboxylase, PDC2) 基因之表現量。兩種水稻的胚鞘及根都因淹水減緩生長,以FR13A 減緩最明顯。通氣組織在淹水期間都有增加,FR13A 中的形成近似於對照組,IR64 則明顯較差。FR13A 中ADH1 及ADH2 在淹水一小時後迅速增加60 至100 倍,IR64 僅增加10 至20 倍。PDC2 在IR64 中表現量的增加幅度較大,但最大值仍小於FR13A 之基礎表現量。由此可知,FR13A 在完全淹水時成長減緩而原有通氣組織則持續生長,且酒精醱酵中的基因有獨特誘導反應,因此耐水性較佳。藉由此研究揭開水稻細胞及分子生物學上的耐水反應策略,將可更精準地改良稻作使其對抗淹水逆境,解決未來因環境造成的糧食危機。Global warming increases the frequency of flooding, which drastically reduces the growth and survival of plants. Although rice (Oryza sativa) appears well-adapted to flooding of roots as it is often farmed in paddies, problems arise when the whole plant is submerged in water. I am interested in the structural and molecular responses that result in different submergence tolerances in rice cultivars. Indica rice FR13A is submergence-tolerant and frequently used in molecular breeding for this trait, while IR64 is a high-yield but submergence-intolerant cultivar. In this study, I monitored the growth rate, aerenchyma formation, and gene expressions of the carbohydrate metabolism in FR13A and IR64 seedlings subjected to submergence for 24 hours, by means of real time RT-PCR and microarray. FR13A had prominently inhibited coleoptile growth and sustained levels of aerenchyma formation whereas IR64 did not. The mRNA levels of alcohol dehydrogenase 1 (ADH1) in FR13A was induced prominently, while ADH2 was induced in IR64 during early hours of submergence. The induction of pyruvate decarboxylase 2 in FR13A was stronger than IR64. The expression of sucrose synthase was similar in both strains. Expressions of the genes involved in anaerobic carbohydrate metabolism were also studied by analyses of microarray data. My findings demonstrate that elongation quiescence, persistent aerenchyma formation and shifts in anaerobic carbohydrate metabolism gene expressions are beneficial strategies of FR13A towards submergence. Through elucidating the molecular basis of coordinating submergence tolerance genes as this study provided, it will be possible to discover multiple traits associated; hence crop improvement for flooding tolerance could be achieved.
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臭氧事件日-氣象與地形對臭氧於近地大氣層之生成與傳輸影響
2001、2002 年監測資料被用以探討風場、時間、地形對臭氧傳輸影響。完成沿海地區與盆地內各二次採樣分析,探討各污染物與風場之垂直變化,及地面臭氧分布與風場變化。結果顯示各月份與全年之日間,其相對濕度與O3 相關度最高,日照次之。提高濕度,最能抑制[O3]。夜間NO 與O3 的相關度最高,濕度次之。臭氧事件日時:(1).11:00 即可產生高臭氧,(2).沿海地區在臭氧事件日仍保持低[O3], (3).盆地效應改變風場,使近山地區[O3]居高不下,(4).因處O3 不斷被吹入,沿海地區傍晚時之[O3]下降速度減緩。臭氧事件日之O3 生成速率R 與消失速率常數L 被求出,其中14:00 後之R 與L 值均由正值轉負值,顯示大氣反應型式明顯轉變。R 與L 值在14:00∼15:00 間最小,係因O3 反應生成光化學煙霧所致。16:00 後另一低R 與L 值,則可能導因O3 與微粒或水份反應。分析結果顯示:(1).污染物會隨高程而略增,最高濃度在300∼500 m 處,(2).各高程大氣均可分析出73 種主要HC,其隨高度之分布被繪出與探討,(3).低層大氣會有較多低分子量HC,而高層大氣則有較多高分子量HC。結果亦顯示: (1).盆地內[O3]、[NO]與[NO2]不僅較沿海地區高,且於各高程之變動亦較大,(2).沿海地區THC、烷、烯類均較高,且隨高程增加而增高,但在盆地內則相反,(3).二地區各高程之鹵化物、芳香族、氧化物與其他有機物之平均濃度相近,但沿海地區之濃度變動較大。探討O3 之二傳輸現象獲知:(1).風吹向盆地內時,[O3]隨風向遞增,R 與L 會由12:00 之正值,轉為14:00 之負值;(2).風由盆地內外吹時,[O3] 隨風向遞減,R 與L 值提前於12:00 即為–202.561 與–1.621,但14:00 時R 與L 值增大為–76.411 與0.244, (3).風向並非決定[O3]的主要因素,地形與環境因素才是。實驗結果證實:(1).不同HC 會影響O3 之生成與消失,(2).改變[NO]o 對[O3]影響不大,但高[NO]會使得[O3]下降略緩,(3).降低HC 與[NOx]雖可使 [O3]略降,但提高濕度最能抑制[O3],(4).在O3 的衰減量上,[O3]隨濕度增加而快速降低,但衰減率則隨[O3]o 的增加而降低。一個臭氧之統計模式被建立,臭氧與水反應速率常數與速率式也被求出。
The monitoring data were used to investigate the effect of surface wind, time, and terrain on the transformation of ozone. The sampling and the analysis in the coastal and in Taichung basin were completed. The vertical distribution of O3, NOX and HC and the different altitude wind were investigated. The contour of O3 and surface wind with 3D map were plotted. The results show that the correlation behaves relativity of relative humidity with ozone is the best, and solar radiation is the next. Enhancing environmental moisture can efficiently decrease ozone concentration. In each ozone event day are: (1) the high [O3] always starts from 11:00, (2) the ozone concentration on the coastal is always low due to the high humidity, (3) the high [O3] in the east of the basin is due to the basin effect which causes changes the surface wind, and (4) in the evening, the descend rate of [O3] in the coastal area is lower because ozone blows into the coastal area. The formation rate (R) and disappear rate constant (L) of the ozone event day were obtained. The values of R and L change from plus to minus before 14:00. The values of R and L are lower at 14:00∼15:00 due to the photochemical smog formation. And another lower R and L value before 16:00 may be due to ozone react with particle or water. The results of analysis indicate that: (1) the concentration increases with increasing altitude, and the maximum is at 300∼500 m height, (2) 73 kinds of hydrocarbons were identified, and the concentration variation with altitude was also investigated, and (3) most of low molecular weight HC are at lower altitude, otherwise high molecular weight HC are at higher altitude. The results also show that: (1) [O3], [NO], and [NO2] on the basin are not only lower than on the coastal, but also their variability of concentration are big, (2) THC, paraffins, and olefins on the coastal are higher than on the basin, and the concentrations increase with increasing altitude on the coastal, but on the basin is decreasing, (3) the average concentrations of halides, aromatics, oxides, and others are similar on both area, but concentration variability on the coastal is obvious. Two types of O3 transformation was investigated, the phenomenon indicts that: (1) when wind blew into the basin, [O3] increased with wind direction. The value of R and L change from positive (12:00) to negative (14:00); (2) when wind blew out of the basin, [O3] decreased with wind direction. The more small value of R (−202.561) and L (−1.621) appeared at 12:00 earlier. But the value of R and L will become bigger to –76.411 and 0.244; (3) ozone concentration does not just dependent on wind direction., topography and surrounding conditionsa are more important effect. The experimental results show that: (1) ozone formation or disappearance depends on different HC, (2) the effect 2 of [NO]o is small for ozone photochemical reaction, but [O3] decreases with increasing [NO], (3) the descend rate of O3 depends on high humidity more than different kinds of HC or [NO], and (4) the descend amount of ozone increases with increasing humidity strongly, and the descend rate of ozone decreases with enhancing [O3]o. A statistical model was developed. The reaction rate and rate constant of ozone reaction with water were also obtained.
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PURPOSE: The purpose of making an Inverter which gets charged with the help of sound energy, produced by speakers for instance, and regenerative shock absorbers which are used in cars so that we can easily charge the inverter with the help of sources which we use in daily life. PROCEDURE: The regenerative shock absorbers are capable of generating electricity when a car moves over bumps. It works by hydraulic fluid passing through a turbine. When the fluid passes through turbine, the turbine turns a small generator and more power is created. A piston is disposed for reciprocating motion within a cylinder as a vehicle’s suspension system deflects. Hydraulic fluid passes through a hydraulic motor to turn its shaft. The hydraulic motor shaft is connected to an electric generator to generate electricity. The second source of energy to charge the inverter is by the help of sound produced in day to day life. Some piezoelectric sensors attached to the board as soon there is a tap or any kind of vibration on the board these and convert them to electrical signals. This means that parasitic energy of busy roads, railroads, footpaths and runways near population centers can be converted into electrical energy that can run public lighting, or fed back into the grid. DATA: The data which have been collected with some experiments is that on an average piezoelectric can produce 330W of power. • When sound pressure is around 62 dB, the frequency is of 102 Hz. • Sound pressure is of 65 dB the frequency is of 500 Hz. Another case when the regenerative shock absorbers come in contact with the piston it produces an average power of 340W-350W. CONCLUSION: With the use age of piezoelectric sensors and regenerative shock absorbers we can produce electricity at a very low cost for inverters; these inverters can provide electrical supplies to the house. The most important reason to make such kind of innovation is one because it is money efficient, second this can come in handy for those who can’t afford to buy inverters at a very high cost and then when charging these inverter these people have to use their electrical supply!
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關於平面上若干點的集中度之定義,一直很少有人予以仔細的探討,因為判別的方法有很多種。本研究是以平面上三點所構成的三角形之三頂點作討論,分別以三角形的面積、周長,內心、外心和重心至三頂點距離,三點的標準差及平均差的概念,作為不同的判別方式,並以動態幾何化版GSP模擬不同的判斷方法做為研究,在利用統計學上的方法,找出哪一種定義方式最為適合、誤差最少的判斷方法。There are many ways to determine the centralness of three dots on a plane; however, no definition study has been applied. In this study, we focus our interests on the entralness of three vertices of a triangle formed from three dots on a plane. Various methods such as the relationship of area, and the distance of the triangle to the centralness of three dots, the distance from the interior point, exterior point, the barycenter to the vertices of triangle, and the standard error and differences average obtained from three dots were determined with a dynamic geometry software GSP, and a statistic method was used to find a least error way to determine the centralness of three dots on a plane.
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聚球藻 RF 一 1 品系生物時鐘之特性
我們成功的用溶氣計偵測到了聚球藻 RF-1 在 28℃下光合作用的概日韻律。和傳統的研究方法比起來,這個方法具有連續偵測的優點,減少因不斷取樣所造成的影響,此實驗可觀察到聚球藻 RF-1 溶氣量之變化圖與一般藻類(如單殼縫藻)不同.在光 /暗條件下 RF-1 之溶氣量的增加與減少均呈週期性變化,而且此變化現象在進入連續照光後仍然可以維持兩個循環以上,這些結果顯示以溶氣計連續偵測聚球藻 RF -1之概日韻律是可行的,而且所得到的變化圖形遠比傳統方法(於不同時間取樣)所得者自然。本實驗同時發現含聚球藻之培養液的溫度,在進入黑暗週期時會有明顯的上升,由於其變化程度比其他藻類明顯,如加以探討應有助於對此藻以及其韻律特性之瞭解。We successfully detected the photosynthesis circadian rhythm of the prokaryote Synechococcus RF-1 under 28℃ by a DO (Dissolved-Oxygen) meter. The advantage of this method, comparing with the traditional methods, is that it can detect signals continuously, reduce the influence of discrete sampling. The DO curves of the Synechococcus RF-1 are different from that of other algae. Under Light/Dark conditions, the DO values of RF-i increased and decreased periodically. The periodic phenomena progressed over two cycles under constant lighting conditions. These results revealed the feasibility of using DO meter to continuous detect the circadian rhythm of the Synechococcus RF-1 The detected DO curves looked more natural than those obtained in the traditional discrete-sampling method. We also found that the temperature of the culture increased in dark cycle. Since the variation is clearer than that of other algae, further investigation will benefit the understanding of the Synechcoccus RF-i and its circadian rhythm.
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一些Moire patterns 的數學性質研究
Moire 為法文,其英譯為watered, 是古代織布技術的一種應用;將印有規律條?的透明薄片重疊時,稍微移動或轉動其中的一片,會形成極大的圖形變化,稱為moire pattern本作品針對三個moire pattern 的數學式加以推導:(一)、兩張透明片各印有等間隔平行線,轉動其中一片使兩線的夾角θ,亮紋垂直距離和暗?垂直距離的比值為tanθ/2tanθ 。(二)、兩張透明片各印有輻射線,重疊後行成圓系,可由代數或幾何加以證明,利用三角函數可推導出此圓系方程式為:x2+{y-rtan[π/2-(θ-?)]}2)]}={rsec[π/2-(θ-?)]}2)]}\r \r (三)、透明片A 印有等間隔平行線,B 印有符合高斯曲線的平行線,AB 重疊時,形成一系列的高斯曲線,AB 的夾角減少時,會增大曲線的曲率,我們進一步討論曲線的曲率和平行線斜率的關係。Moire is the French word “watered” and refers to an ancient technique employed in cloth making. The moire occurs whenever two or more transparent sheets with periodic strips on them are superposed. The characteristic of moire patterns is the fact that a slight shift of sheets will create dramatic alternations in the observed patterns. In the present report, We derive the equations of three different moire patterns. First of all, take a sheet with equal spaced straight lines and placed it on top of another identical sheet. They are made to intersect and form an angle of θ. As the angle changes slightly, it produces huge changes in the spacing of moire fringes. We can derive a formula related to the interfringe distance. The ratio of bright fringes and dark fringes is tanθ/2tanθ.Secondly, two transparent sheets with radial lines on them are overlapped, forming a pattern similar to the lines of force between point charges. We can find that the pattern is a series of circle by means of algebraic and geometric proofs. And proven by trigonometric functions, we canconclude that they satisfy the equation :x2+{y-rtan[π/2-(θ-?)]}2)]}={rsec[π/2-(θ-?)]}2)]}\r Thirdly, a set of lines of equal spacing is overlapped with a second set of lines whose spacing are derived from a Gaussian curve. A series of Gaussian curves is reproduced in a moire pattern. Reducing the angle of intersection between the two figures steepen the curvature. We discussed the relation between the curvature and the slope of inclined lines.
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以數位像素分析法探討兩棲類變色行為之研究
本研究是以數位科技改良生物實驗上的傳統比色法,並探討中國樹蟾及台北樹蛙的變色行為。有些兩棲類有變色行為,這是眾所皆知的現象,過去一向採用比色法判斷生物體的體色變化;然而,以人眼去測定色差,會有相當大的主觀誤差。本研究及是探討以數位科技量化並比較實驗對象的變色情形。在研究過程中,用電腦量化數位相機所拍攝的影像,以像素的方式呈現出來,得以取的準確且客觀的實驗數據,並加以解析,也經由此一實驗方式,交叉比較中國樹蟾及台北樹蛙在不同環境和不同時間下的變色情形。結果顯示:樹蛙變色和環境顏色及時間有密切關係,且在短時間內以數位量化的方式解析其變色行為的方法極為可行。The purpose of this study is to investigate the color variation behavior of tree frogs using an image processing technology. It is known that some of the amphibians have the ability to change their skin color under different circumstances. In the past, a conventional “color comparison” method was frequently used whenever it comes to analyzing the color variation of organisms. However, it is realized that evaluation of color difference by human eyes is extremely unreliable. An advanced image processing technology is thus suggested to quantify the variation of skin color of tree frogs under different conditions in this experiment. Accurate quantification can be determined from the pixel number of exhibited digital images during the experiment. The color variation of different species of frogs (Hyla chinensis & Rhacophorus taipeianus) is also compared under different parameters. It is found that the color variation of tree frogs is closely related to the color of back ground as well as time. The proposed digital quantification technique has been successfully demonstrated to be an effective method for analyzing the color variation of frogs within a very short time.
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本次研究利用Web Sevrice軟體系統和Microsoft SQL Server的全文搜尋(Full-Text search)並藉由使用者選擇的圖片組合排列從系統資料庫中檢索比對,篩檢出正確或者最相似的餐點。Web Service 是建立於網際網路上之系統架構,且為一個具開放性、分散式的軟體元件,其基礎建立在HTTP、XML、SOAP、WSDL和UDDI等標準協定上,此研究即是透過Web Service系統與資料庫伺服器的連結進行。很多數位化資訊是以沒有結構化的文字資料,存成純文字檔及格式化的文件。這一類的文字資料儲存在檔案系統,而許多組織現在將這一類資料儲存到關聯式資料庫。這樣做就可以廣泛利用資料庫的好處-包括高效能存取、查詢能力、對使用者而言簡單的應用程式使用者介面,以及安全的遠端存取、查詢能力、對使用者而言簡單的應用程式使用者介面,以及安全的遠端存取。運用此功能即可在配對完成時搜尋最佳的結果並加以排名。「打破語言隔閡-以圖點餐之數位餐廳 」不只消除語言隔閡,更讓點餐手續方便許多,並讓錯誤的可能性降低。另外圖片資料庫的增減簡單快速、介面明瞭易懂。希望這些應用及創新能讓人們用餐方便及餐廳事業蓬勃發展。This research uses Web Service software system and Microsoft SQL Server. To choose the correct or the most similar meals by checking the combination and arrangement of the pictures chosen by users form the system data banks. Web-Service is a system structure establishes at the Internet. And its an open, dispersed software part. Its foundation is established at the standard agreement of HTTP,XML,SOAP,WSDL, and UDDI. The research proceeds by linking Web Service system and data-bank server lots of digital data are stored as words files and stylization documents without any structural words data. This kid of ward data are stored at file system, and many organized systems now store them at connected data banks. By doing this, people can make the most advantage of data banks- including efficient access, inquiry abilities, simple applies user’ interface, and safe remote access. People can find the best results and place them when this function finishes matching. It can not only eliminate language barriers but also make order procedure more convenient. It can also lower the mistake possibilities. In addition, we can increase or decrease pictures of data banks easily and fast. We can make the interface understood easily. We hope the applications and innovations can make it more convenient for people to order meals. And the business of restaurant can be developed successfully.
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